BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	4,479,395	T→G	14.9%	*198G (TGA→GGA)	Y75_RS22170 →	TetR/AcrR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,479,395	0	T	G	14.9%	227.0 / 2.9	127	*198G (TGA→GGA)	Y75_RS22170	TetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (38/63); new base G (22/2); total (62/65)
Fisher's exact test for biased strand distribution p-value = 1.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

ATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGA  >  NC_007779/4479162‑4479633

aTAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGc                                                                                                                                                                                                                                                     <  1:532974/239‑1 (MQ=255)

     cATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGcc                                                                                                                                                                                                                                               <  1:1040794/239‑1 (MQ=255)

      aTGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGGGAGCACAAATATTTTTTA‑GGGGGTATGC‑ggg                                                                                                                                                                                                                                                 >  1:2039910/1‑236 (MQ=255)

         aTCCACAAGCGCGTGATATTGCCGTTCAATATTATTACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTATGTGCAACAGCTGCAATGGC‑GG‑GGGAGGCGGAAAACACTATTATTGT‑GGGGAGGAGC‑GGGGCTTg                                                                                                                                                                                                                                            >  2:517644/1‑238 (MQ=255)

              caAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGGTAAAAAAAAATCTTTA‑GGGGTGAGGC‑GGGCCTTGGcc‑cca                                                                                                                                                                                                                                     >  2:304300/1‑237 (MQ=255)

                    cgTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAAATATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCg                                                                                                                                                                                                                               >  1:225919/1‑239 (MQ=255)

                    cgTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCAATTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGAAAAAAACTATTCTTTA‑GGAGGGATGC‑GGGCCTTGGCC‑CTGAAgggg                                                                                                                                                                                                                               >  1:1682975/1‑237 (MQ=255)

                    cgTGATATGGCCGTTCAATATTATCACGCCGCTGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GGAGGGAACGAATAAAAACTATTCTGAG‑GGGGTAATGC‑GGGCCTCAACC‑CGagcacc                                                                                                                                                                                                                                >  2:1918994/1‑234 (MQ=255)

                             ccctttaaaTTTTATCCCGCGACCGAAACGCCCATTTGTGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTAGGAGTCCCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGGGGGGCACTCT‑AAAGAACAACTCTGTGAAACAGCGGCAATGGG‑GG‑GGGAAGCGATAAAATCTATTCTTGG‑GGAGTGAGGG‑GGGCCTTGACC‑CGAAGGGCGGG‑AAAgcgc                                                                                                                                                                                                                     <  2:1192286/232‑1 (MQ=255)

                             cccatacAATATTACCACGCCGCAAAAACCACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGGGTCCCGTGATGTCTGGGCTTTCTGCGAAGGCAGGAGGGGGGCACTCT‑ATAGAACAACTTTGGGCAACAGCGGCAAGGGC‑GG‑GGGAAGCAAAAAAAACTATTTTTGA‑GGAGTGATGC‑GGGCCTTGCCC‑CGAAAGGCGGG‑AAAgcgc                                                                                                                                                                                                                     <  2:1561804/233‑1 (MQ=255)

                                 ttCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGCGGG‑GGGAGGGGGAAAAAAATATT‑TTGAGGGAGGGAGGA‑GGGCTTTGACCGAGAAAGGGGGA‑AAAgcacgt                                                                                                                                                                                                                   >  2:1014063/1‑235 (MQ=255)

                                 ttCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGGTAAAAAATATTCTTGA‑GGAGGGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑GAAACGGCTg                                                                                                                                                                                                                  >  1:1047180/1‑238 (MQ=255)

                                 ttCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑GAAGCGCCTg                                                                                                                                                                                                                  >  1:1930524/1‑238 (MQ=255)

                                   cAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAATATTTTTGA‑GGGGTGATGC‑GGGCCTTGACC‑CGGAAGGCGGG‑AAAGACCccgcc‑g                                                                                                                                                                                                              >  1:2070872/1‑239 (MQ=255)

                                     atatTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GAt                                                                                                                                                                                                            <  1:576473/239‑1 (MQ=255)

                                         tctCACGCCGCGAAAACGACCATTTATGACTACATCGCCGGGCGGCATCCACAACGCGCACAAGGTGTGACTGATTTTATGAGAACCGTGATGTCTGGGCTTTCTGCGAGGGCACGAGAGGGGCACTAT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGt                                                                                                                                                                                                        <  2:1973054/237‑1 (MQ=255)

                                         tagcACGCAGCAGAAACGACCATTTATGACTACTTCGCCAGGCGGCATCCACAACGCGCACAAGGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTGTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGt                                                                                                                                                                                                        <  1:1158660/236‑1 (MQ=255)

                                         taccACGCGGAAAAAACGACCTTTTATTACTACATCGCCGGGCGGCATCCACAAGGCGCACAATGTGTGCCTGATTTTATGAGTACCGGAATGTCTGGGCTTTCGGCGAAGGCACGAGGGGGGCACTCT‑ATAAAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGCCC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGt                                                                                                                                                                                                        <  2:2127605/236‑1 (MQ=255)

                                           tCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAATATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGAGGG‑GAAACGCCTGGC‑CATAAGTtg                                                                                                                                                                                                      >  1:793700/1‑239 (MQ=255)

                                           tCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAGGCGGTAAAAACCAATCTTGC‑GGAGTTCTGCGGGGCTTTGCGC‑CAAACGGGGGG‑AAAG‑GCCTGGC‑GAGAAgacg                                                                                                                                                                                                      >  2:147258/1‑236 (MQ=255)

                                              cgccgcAGAAACGACAATTTATGCCTACATCGCAAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTCCCGTGATGTCTGGGGTTTCTGCGAAGGCACGGGGGGGGCACTCT‑ATAAAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGa                                                                                                                                                                                                   <  2:2039910/239‑1 (MQ=255)

                                                ccgcAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAACATTTTTGT‑GGGGTGATGC‑GGGGCTTTAGC‑CGAAAGGCGGG‑GAAGAGCCCGCC‑TAGAAATTGTGTTa                                                                                                                                                                                                 >  2:944032/1‑239 (MQ=255)

                                                ccgcAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGGGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAACGCCTGCC‑GATAAGTTGTGATa                                                                                                                                                                                                 >  1:1546110/1‑239 (MQ=255)

                                                ccgcAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGAAAAAAACTATTCTTGA‑GGGGTGATGC‑GGGCCCTGACC‑CGAAAGGCGGG‑AAAGAGCCTGCC‑GATAAGTTGTGATa                                                                                                                                                                                                 >  1:938068/1‑239 (MQ=255)

                                                 cgcAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATaa                                                                                                                                                                                                <  1:2046451/239‑1 (MQ=255)

                                                             cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAAAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGG‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAACGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCa                                                                                                                                                                                    <  2:512583/239‑1 (MQ=255)

                                                              aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCTTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCAc                                                                                                                                                                                   <  1:832307/239‑1 (MQ=255)

                                                              aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCAc                                                                                                                                                                                   <  1:1907175/239‑1 (MQ=255)

                                                               tttATGATTACATCGCCAGGGGGCATCCACAACGCGCACAAGGGGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATaa                                                                                                                                                                                                <  2:1556179/225‑1 (MQ=255)

                                                                    gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAAATACTATTGA‑GGAGGGGTGC‑GGGGCTTGACC‑CGAAAGGAGGG‑GAAGCGACCTCC‑GATAAGGTGTGATGAGACAAAAAATCaagtcgtt                                                                                                                                                                             >  2:1350918/1‑232 (MQ=255)

                                                                     aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑CATAAGTTTGGATAAGACAAAAATTTACGC‑GTT‑aa                                                                                                                                                                          >  1:274021/1‑239 (MQ=255)

                                                                        aCATCGCCAGGCGGCATCCACAACGCGCACAATGTGGGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGc                                                                                                                                                                       <  1:73230/239‑1 (MQ=255)

                                                                         cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGGTGTGGTAAGACAATAAtt                                                                                                                                                                                      >  1:1556179/1‑225 (MQ=255)

                                                                         cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGGGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCCGCC‑GATAAGTTTTGATAAGACAATAATTCAAGC‑GGT‑AAGGCt                                                                                                                                                                      >  1:1384478/1‑239 (MQ=255)

                                                                          aTCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGGTAAAAAATATTCTTGA‑GGAGGGAGTA‑GGGCCTTGGCC‑CCGAAGGCGGG‑GAAAGGCCCGGC‑GCGAAGGTGGGGTTAGAAAAAAATTAACGC‑GGT‑agagcat                                                                                                                                                                     >  2:2119019/1‑233 (MQ=255)

                                                                             gCCAGGCGGCATCCAGATCGCGCACAATGTGGGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCg                                                                                                                                                                  <  1:69499/239‑1 (MQ=255)

                                                                                   cggcATCTACAACGCGCACAATGTGTGATTGATTTTATGAGTACCGTGATGTCTGTGCTTTCGGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTGTGTGCAACAGCTGCAAGGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGATTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGaa                                                                                                                                                               <  1:1175636/236‑1 (MQ=255)

                                                                                      cATCCACAACGCGCACAATGTGTGACTGATTTTATTAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGAt                                                                                                                                                          <  1:1685268/238‑1 (MQ=255)

                                                                                                  gcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑tat                                                                                                                                            <  1:147258/239‑1 (MQ=255)

                                                                                                  gcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑tat                                                                                                                                            <  1:1696515/239‑1 (MQ=255)

                                                                                                      aaTGGGGGACTGATTTTATGAGTACCGGGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCa                                                                                                                                                                                    <  1:946912/198‑1 (MQ=255)

                                                                                                       aTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAAATATTCTTTA‑GGGGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAAACGCTTCC‑GAAAAGATGTGGTAAGAAAAAAAATCCCGC‑GGT‑AAGGCTAACGGGAATGGTTATTTTTTCG‑Taaaaata                                                                                                                                       >  2:1356643/1‑236 (MQ=255)

                                                                                                               cTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATg                                                                                                                               <  1:892798/239‑1 (MQ=255)

                                                                                                                  aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGGGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGAGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACCATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATagaaaagaaa                                                                                                                            >  1:1792265/1‑239 (MQ=255)

                                                                                                                  aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGGTAAAAAATATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGGGGG‑AAAGCGCCCGCC‑GATAAGTTGTGATAAGACAAAAATTCACGC‑GTT‑AAAGCTAGCGGAAATGATTATCTTTTCG‑TATAACGATagaaaagaaa                                                                                                                            >  1:2122791/1‑239 (MQ=255)

                                                                                                                  aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCa                                                                                                                                                                                    <  1:1776535/186‑1 (MQ=255)

                                                                                                                    tttttggttACCGTGATGTCTGGGTTTTCTGCGAGGGCACGAGGGGGGAACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAGGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCTTTGACC‑AGAAAGGCGGG‑AAAGCGCCTGCC‑GATTAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACg                                                                                                                          <  2:1792265/231‑1 (MQ=255)

                                                                                                                    tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACg                                                                                                                          <  1:1588531/239‑1 (MQ=255)

                                                                                                                    tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACg                                                                                                                          <  1:930242/239‑1 (MQ=255)

                                                                                                                        tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAAGAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACgttgt                                                                                                                      <  1:1014063/239‑1 (MQ=255)

                                                                                                                        tGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGAACTCT‑ATAGAACAACGCTGTGCAACAGCTGCAAGGGC‑GG‑GGGAAGGGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTGGCCC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACgttgt                                                                                                                      <  1:805299/239‑1 (MQ=255)

                                                                                                                        agAGTACCGTAATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACgttgt                                                                                                                      <  1:944032/238‑1 (MQ=255)

                                                                                                                           gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGAACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGttt                                                                                                                    <  1:304300/238‑1 (MQ=255)

                                                                                                                                 tGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGG‑AGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCCTGACC‑CGAAAGGAGGG‑GAAACGACCGCC‑GATAAGATGTGGTAAGACAAAAAATCACGC‑GTT‑AAGGCTAGCGGGATTGATTTTCTTTTCG‑TATAACGAGAAAAATGAAAAGTTGTGttattttat                                                                                                            >  2:1727544/1‑236 (MQ=255)

                                                                                                                                  gATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAg                                                                                                            <  1:1355832/239‑1 (MQ=255)

                                                                                                                                   atttttGGGCTTTCTGGGGAGGCCCGAGAGGGGTACTCT‑AGAGAACAATTCTGGGCAGCAGCTGCAGTGGC‑GG‑GGGAAGGGAAAAAATGTATCCTATA‑GGAGTGATGC‑GGCTGTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAg                                                                                                            <  1:1248649/233‑1 (MQ=255)

                                                                                                                                    tGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGGGATAAAAAATATTCTTGG‑AGGGGGAAGCGGGGGCTGTGCC‑CCAAAGG‑GGG‑AAAGCGGCTGCCAGACAGGTTG‑GGGAAGACAACAATTCCCACGGTA‑AAGGCGA‑CGGGAATGAGTATGTTTTAT‑TATAAAGAAGAAAAATAAACAGTGTTTTAATTAAggg                                                                                                          >  2:310269/1‑238 (MQ=255)

                                                                                                                                      tCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGGGGTAAAAAATATTTTTGA‑GGGGGGAAGC‑GGGCCTTGGCC‑CCAAAAGCGGGAAAAGGGCTTGCG‑GA‑AAGTTGGGGTAAAAAAAAAAATCACCC‑GTTAAAAGCAGGCGGAA‑TGATTATTTTTTTG‑TATAAAAAAAGAAAAGAAAACTTGTTtttattaatgtag                                                                                                        >  2:1603163/1‑234 (MQ=255)

                                                                                                                                                      gggaaggagaGGGG‑ACTTTAATAAAACAACTCGGTGAAACAGCTGCAAGGGC‑GG‑GGGAGGCAAAAAAAACTATCTTTGA‑GGAGGGATGC‑GGGCTTTGACA‑AGAAAGGCGGG‑AAGGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTGTCG‑GATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑atcat                                                                                      <  2:1204236/232‑1 (MQ=255)

                                                                                                                                                          aCGAGAGGGGCACTCT‑ATAGAACAACTTTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCATTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGaa                                                                                 <  2:940659/239‑1 (MQ=255)

                                                                                                                                                               agGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCGGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGGCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAatat                                                                             <  1:2194196/238‑1 (MQ=255)

                                                                                                                                                               agGGGCACTCT‑AAAGAACACCTCTGTGCAACGGCTGCAAGGGC‑GG‑GGGAAGCGATAAAAACTATTCTGGA‑GGAGTGATGC‑GGGCCTTGACC‑CAAAAGGCGGA‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGAc                                                                                                                                                                                             <  2:295274/132‑1 (MQ=255)

                                                                                                                                                                   gCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAACAATTTTTTA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAACGCCTGCC‑CGTAAATTTGGGTAAAACAAAAATTCACGC‑GGT‑AAGGCCAGCGGGAATGAATTTCTTTTTG‑TATAACTGTAAAAATGAAACGGTGGTTTTAATAAAGAGT‑GGGAAAGAATG‑A‑CATCGGAAAAATTTAtt                                                                        >  2:1098431/1‑238 (MQ=255)

                                                                                                                                                                    cACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GAt                                                                                                                                                                                                            <  1:693761/112‑1 (MQ=255)

                                                                                                                                                                         t‑ataGAACAATTCTGTGCAGCAGCTGCAATGGC‑GG‑GGGAGGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCg                                                                                                                                                                  <  1:1364313/147‑1 (MQ=255)

                                                                                                                                                                         t‑ataGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑GAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCAc                                                                                                                                                                                   >  1:295274/1‑132 (MQ=255)

                                                                                                                                                                         t‑ataGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAgg                                                                                                                                                                        <  1:1491740/141‑1 (MQ=255)

                                                                                                                                                                         t‑ataGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACg                                                                                                                          <  1:1573795/186‑1 (MQ=255)

                                                                                                                                                                         t‑ataGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACg                                                                                                                          <  1:1479890/186‑1 (MQ=255)

                                                                                                                                                                             aGAACAACGCTGTGCAACAGCGGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGc                                                               <  1:517644/239‑1 (MQ=255)

                                                                                                                                                                               aacaacTCTGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAACTATTCTTGA‑GGAGGGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑GAAACGCCTGCC‑CATAAGGTGTGAt                                                                                                                                                                                                  >  2:693761/1‑112 (MQ=255)

                                                                                                                                                                               aacaacTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAAACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATTTTCAGCCg                                                             >  1:241323/1‑239 (MQ=255)

                                                                                                                                                                                acaacGCTGTGCAACGGCTGCAATGGC‑GG‑GGGAAGCGAGAAAAACTATTCTTGA‑GGAGTGATGC‑GGTCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTGAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGcc                                                              <  1:1045817/237‑1 (MQ=255)

                                                                                                                                                                                  aacTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTGGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCTGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATCCATAATCTTCAGCCGTgg                                                          <  1:230830/239‑1 (MQ=255)

                                                                                                                                                                                   attctGGGAAACGGCGGCAAGGGC‑GG‑GGAAGGCGAAAAAAACTATTCTTGA‑GGAGTGAGGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGc                                                         <  2:274021/237‑1 (MQ=255)

                                                                                                                                                                                   acTCTGTGCAAAAGCTGCAATGGC‑GG‑GGGAGGCAATAAAAACTATCCTTGA‑GGAGTGATGG‑GGGCCTTGACC‑CAAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGc                                                         <  2:793700/239‑1 (MQ=255)

                                                                                                                                                                                   acTCGGTGCAACAGCGGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGc                                                         <  2:1825570/239‑1 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAATATTTTTTA‑GGAGTGATGC‑GGGGCCTGTAC‑CGAAAGGGCGG‑AAAACGGCTGCC‑GAGAAGGTTTGATAAGACAAAAAATTACGC‑GGT‑AAGGGCAACGGGAATGGTTATTTTTTTGTTTTAACATTAAAAATAAAAGGTTGTTTTATTTAA‑GAGTGGGAAAAC‑ATG‑AT‑AACGGAAATAATAATAAtttttcatcctggga                                                        >  2:1045817/1‑228 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAATATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAACGCCTGCC‑GATAAGTTGTGATAAGACAATAAATTACGC‑GTT‑AAGGCTAGCGGGATTGATTTTTTTTTTG‑TCTAAAGATAGAAATGAAACGTTGTTTTTATTAAGGAGT‑GGAAAAA‑AAT‑ATCATCGGAAAAATTTATAATCTTCAACCGttgtc                                                        >  2:1922812/1‑235 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAATATTCTTGA‑GGAGGGATGC‑GGGCCCTGACC‑CGAAAGGGGGG‑AAAAAGCCCGGC‑GATAAGTTGTGAGAAGACAATAAATTACGC‑GGT‑AAAGCTAGCGGGAATGATTATTTTTTTG‑GATAAAGAGAGAAATAAAAAGTTGTTTTAATTAAGGAGG‑GGAAAAA‑ATG‑AACATAGGAAAAATTTCTAATCTTCaaaccgggc                                                         >  2:1754931/1‑230 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAAATCTTTTTA‑GGAGGGATGC‑GGGTCTTGACT‑CGAAAGGGGGG‑GAAAGGCCTGCC‑GATAAGGGGTGATAAGATAATAATTACAGC‑GCT‑AAAGCTTGCGGGACTAATTTACTTTTCT‑TTTTATACGAGAAAGAAAACGTTGGTTTTATTATGGTGT‑GGATTAT‑ATA‑GACAGAGGAAATATTCATTATCtttacaccttgct                                                        >  2:819453/1‑228 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAAAAATTCTTTA‑GGGGGGATGC‑GGGGCGTGAGC‑CGAAAAGCGGG‑AAAAAGCCTGCC‑GCTAAGATGTGATAAGACAAAAATTCACGG‑GTT‑AAAGCTAGCGGAAATTATTATCTTTTTG‑TATAACAATAGAAATGAAAAGGTGTTTTTATTAAGGAGT‑GGAAAAG‑ATG‑AAACTCGGAAAAATTTATAATATTCAACCGTgggt                                                        >  2:2046540/1‑237 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATTAAAACAATTCTTGT‑GGCGTGATGC‑GGGCCTTGAAC‑CGAAAGGCGGG‑AAAACGCATGCC‑GAAAAGTTGTGATAAAACAATAATTCACGC‑GTT‑AAGGCTAGCGGGAATGTATATCTTTTTG‑TATAACGATAGAAATGAAAAGTTGTTTTAATTAAGGAGT‑GGAAAAA‑ATT‑ATCATAGGAAATATTAATAATATTCAGCCggtgt                                                         >  2:587034/1‑234 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTTA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAAAGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATATTCAGCCGTGGCt                                                        >  1:2006154/1‑239 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTTA‑GGAGGGATGC‑GGGCCTTGACC‑CCAAAGGCGGG‑AAAACGCCCGCC‑GATAAGGTGTGATAAGACAATAAATTACGC‑GTT‑AAGGCTAGCGGAAATGATTTTTTTTTCT‑TATAACAATAGAAATGAAACGTTGTTTTAATTTAAGAGT‑GGGAAAA‑AGA‑ATCATCGGAAAAATTATTAAACTTTACACGTggg                                                         >  2:2141896/1‑237 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCgg                                                                                                                                                                 <  2:1735963/138‑1 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCgg                                                                                                                                                                 >  1:1735963/1‑138 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTttaatta                                                                                                              >  1:59717/1‑188 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGGAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCt                                                        >  1:1661046/1‑239 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAAATATTCTTGA‑AGAGTGATGC‑GGGCCTTGACC‑CGAAAAGCGGG‑GAAGCGCATGCC‑GATAAGGTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGGAATGGTTATTTTTTCG‑TATAAAGATAGAAATGAAACGGTGGTTTAATTTATGAGT‑GGAAAAG‑ATG‑ATCATCGGAAAAATTAAAAATTTTCAACCGTTGCt                                                        >  1:957163/1‑239 (MQ=255)

                                                                                                                                                                                    ctctGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCCATAAAAAAAATTCTTGA‑GGAGGGATGC‑GGGGCTTGAGC‑CCAAAAGCGGG‑GAAAAGCCTGGC‑GGTAAATTGTGATAAAACAAAAAATCACAC‑GGT‑AAAGGCAGCGGGATTTATTTTTTTTTTG‑TATAAAAATAAAAAATAAAAGATTTTTTTAATAAAGAGT‑GGAAAAA‑AAGAATCATC‑GAAAAATTAATAAACtttatgc                                                              >  2:1430616/1‑228 (MQ=255)

                                                                                                                                                                                         tgCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GGT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcccc                                                   >  1:1187399/1‑239 (MQ=255)

                                                                                                                                                                                         tgCAACAGCTGCAATGGC‑GG‑GGGGAGCGATAAAAACTATTCTTGA‑GGAGTGAAGC‑GGGGCCTGGCC‑CGAAAGGCGGG‑AAAACGCCTGCC‑GGGGAGTTGTGGTAAGAAAAAAAATCAACC‑GGT‑AAGGGCAGGGGGAATGAATATTTTTTTT‑TATAACCGTAAAAATGAAAAGATGTTTTTATTTAGGGGT‑GGAAAAA‑ATT‑ATCATCGGGAATATTAATAATTTTCACCCgggggcaacc                                                    >  2:1619778/1‑232 (MQ=255)

                                                                                                                                                                                         tgCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAAAAATTTTTGA‑GGAAGGATGC‑GGGGCTTGGAC‑CGAAAGGGGGG‑AAAACGCCTGGC‑GGTAAGGTGTGATAAGAAAAAAAATCACGC‑GTT‑AAGGCTAGGGGGAATGGTTTTTTTTTTG‑TATAACAATAAAAAAAAAAAGGTGTTTTATTTAAGGAGT‑GGAGAAA‑AAG‑AACATATGAAAAATTTATAATCTTTAGCCGTGGcgaacc                                                    >  2:616722/1‑233 (MQ=255)

                                                                                                                                                                                                       agggggg‑gggAAGAGAAAAAAACTGTTCTTGG‑GGATTGATGG‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa                                      <  2:2056092/234‑1 (MQ=255)

                                                                                                                                                                                                            gg‑gggAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGa                                 <  1:2046540/239‑1 (MQ=255)

                                                                                                                                                                                                             g‑gggAGGCGATAAAAACTATTTTTGA‑GGGGTGAGGG‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGTGCCTGCC‑GATAAGTTGTGAGAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGa                                 <  2:938068/238‑1 (MQ=255)

                                                                                                                                                                                                             g‑gggAAGCAATAAAAACATTTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGa                                 <  1:2141896/238‑1 (MQ=255)

                                                                                                                                                                                                                                       a‑ggagTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACg       >  1:194955/1‑239 (MQ=255)

                                                                                                                                                                                                                                          gagTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGc     >  1:1929264/1‑239 (MQ=255)

ATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCACGCCGCAGAAACGACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCT‑ATAGAACAACTCTGTGCAACAGCTGCAATGGC‑GG‑GGGAAGCGATAAAAACTATTCTTGA‑GGAGTGATGC‑GGGCCTTGACC‑CGAAAGGCGGG‑AAAGCGCCTGCC‑GATAAGTTGTGATAAGACAATAATTCACGC‑GTT‑AAGGCTAGCGGAATTGATTATCTTTTCG‑TATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGT‑GGAAAAG‑ATG‑ATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGA  >  NC_007779/4479162‑4479633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`