BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	4,631,348	A→C	9.5%	T253P (ACC→CCC)	radA →	DNA repair protein RadA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,631,348	0	A	C	9.5%	587.2 / 3.3	214	T253P (ACC→CCC)	radA	DNA repair protein RadA
Reads supporting (aligned to +/- strand): ref base A (116/66); new base C (5/22); total (121/93)
Fisher's exact test for biased strand distribution p-value = 1.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

GGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGT  >  NC_007779/4631113‑4631583

ggTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGGTGCCGAC‑TCCCGTTTTCGCACCt                                                                                                                                                                                                                                           >  1:18500/1‑239 (MQ=255)

ggTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCGTATTTGACACGCTTCGCCACAACGCGCGGTGGGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCCCAAAGGCTTGGACAC‑TCTATAGCCTGGTCGGTGCTTTTGGAGGGGCGTGCCGGC‑CCCCGTTTTCCCCCCt                                                                                                                                                                                                                                           >  2:703343/1‑239 (MQ=255)

     ttGACGCGATCCGGGGGAGGCATATGGGGGATGTACAGTCGTCGCCTGTTAGCGGGGCGCAGGGGCGTGAAACGGCGGCTTATTTGACCCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTgcgc                                                                                                                                                                                                                                      <  2:458122/239‑1 (MQ=255)

      tGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCa                                                                                                                                                                                                                                     <  1:1583667/239‑1 (MQ=255)

             aTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTTACACGCTTCGCCAAAACGCGCGGTGTGGCGGTTGTCATGGTGGGGCCCCTAACCCAAAAAGGGTCGCCGGGT‑GGCCCCAAAGGGCTTGGACAC‑CGGATTTACCGTTTGGGGCTCTTGGGTGGCGATTCCGGCACCCCTTTTTGGCACCttcgccgctctaa                                                                                                                                                                                                                               >  2:918132/1‑228 (MQ=255)

                cAGGTGATGCAAATGGCGGTGGTACAGTCACCGCCTGGTGGCGTGGCGCGGGTGCGTAAAACGGCGGCTTATTTGACACGTTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGCCTGTTCGGTGCTTTTGGAGGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATaaaaa                                                                                                                                                                                                                           <  2:1977107/239‑1 (MQ=255)

                       tGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTc                                                                                                                                                                                                                    >  1:1920011/1‑239 (MQ=255)

                       tGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAA‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTc                                                                                                                                                                                                                    >  1:13320/1‑239 (MQ=255)

                       tGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGGGTGGCGATTGTCATGGGGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTc                                                                                                                                                                                                                    >  1:1341436/1‑239 (MQ=255)

                        gCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCCAAACGCGCGGTGTGGCGTTTGTCATGGTGGGGCCCGCAACCCAAAAAGGGTCCCTGGCT‑GGCCCGAAAGTGCTGGAACCC‑AGTATCGACCGTTCGGGGCTTTTTGATGGCGATGCCCGC‑TCCCGTTTTCGCCCCCTGCGCCGCCCTAAAAAACCCTTcc                                                                                                                                                                                                                   >  2:2107588/1‑238 (MQ=255)

                        gCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCCGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGAAGGTTCGCTGGCT‑GGCCCGAAAACGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCCTCg                                                                                                                                                                                                                   >  1:1810034/1‑239 (MQ=255)

                           tatGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGACGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGgcg                                                                                                                                                                                                                <  1:654297/239‑1 (MQ=255)

                             tGGCGGATGTACAGTCAGCGCCTGGTAGCGGGGCGCGGGTGCGTGAAACGGCGGCGTATTTGACACGCTGCGCCAAAACGGGCGGTGTGGAGATTGTCATGGTGGGGCACGTACCCAAAGATGGTTCGCGGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCCCCTGTTCGGTGCTTTTGGATGGCGATGCCGCC‑TCCCTTTTTCGCACTTTGCGCAGCCATAAAAACCGCTTCGGcgcg                                                                                                                                                                                                              <  2:1515635/239‑1 (MQ=255)

                                  gATGTAAAGTCATGGCCTGGTGGCGGGGCGCGGGTGCGTAAAACGGCGGCTTATTTGACACCCTTCGCCAAAACGCGGGGTGTGGGGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGTT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGa                                                                                                                                                                                                          <  2:845193/238‑1 (MQ=255)

                                        cAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGGGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCCGGCATAAAAACCGCTTCGGCGCGGTGAATGAGcc                                                                                                                                                                                                   >  1:438643/1‑238 (MQ=255)

                                         aGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAA‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTg                                                                                                                                                                                                  >  1:1822111/1‑239 (MQ=255)

                                           tCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAg                                                                                                                                                                                                     >  1:501708/1‑234 (MQ=255)

                                            cATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTggg                                                                                                                                                                                                <  1:1686871/238‑1 (MQ=255)

                                            cATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAACATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGGGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGc                                                                                                                                                                                               >  1:2142229/1‑239 (MQ=255)

                                             aTCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGc                                                                                                                                                                                               >  1:865683/1‑238 (MQ=255)

                                               cGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCG‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCGGGGCg                                                                                                                                                                                              >  1:2227712/1‑237 (MQ=255)

                                                     gTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcgcg                                                                                                                                                                                      >  1:518734/1‑239 (MQ=255)

                                                       aGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGa                                                                                                                                                                                     <  1:1963727/238‑1 (MQ=255)

                                                         cGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGATACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGGC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGCGAATGAGCCGGGCGTCCTCGCGATg                                                                                                                                                                                   >  2:572296/1‑238 (MQ=255)

                                                          ggggCGAAGGTGGGTAAAACGGCGGTTTTTTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCCCGTACCCAAGGATGGTTCGCGGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTTTCGACTGTTCGGTGCTTTTGGATGGCGATGCCGCC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑a                                                                                                                                                                                 <  2:1075017/236‑1 (MQ=255)

                                                          gTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACCCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGACGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑Ac                                                                                                                                                                                <  1:1530291/239‑1 (MQ=255)

                                                           tGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACCC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCCCCTTGCGCCGCCATAAAAACCCCCTCGGCGCCGTGAATGGGCTGGGCGTCTTCGCGCTG‑Ac                                                                                                                                                                                >  2:1051969/1‑238 (MQ=255)

                                                           tGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑Ac                                                                                                                                                                                <  1:145268/238‑1 (MQ=255)

                                                           tGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGAGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑Acc                                                                                                                                                                               >  1:1985605/1‑239 (MQ=255)

                                                             gcgcAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGTC‑TCCCGTTTTCGCACCTTgcg                                                                                                                                                                                                                                       <  1:613893/182‑1 (MQ=255)

                                                                   gTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGAGGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCCGCCATAAAAACCGCTTCGGCGCGGGGAATGTGCTGGGCGTCTTCGCGATGTACCG‑ACAggg                                                                                                                                                                       >  2:610442/1‑239 (MQ=255)

                                                                    tGCGTGAAACGGCGGCTTATTTGACCCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGACCAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAggg                                                                                                                                                                       <  1:1678288/238‑1 (MQ=255)

                                                                    tGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTACCCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAggg                                                                                                                                                                       <  1:1050300/238‑1 (MQ=255)

                                                                     gggTGAAAGGGGGGTTTATTTGACACGCTTCGCAAAAGCGCGCGGTGTGGCGATTGTCATGGTGGGGCAGGTAACAAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCGGTTTTCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAgggg                                                                                                                                                                      <  2:1165026/236‑1 (MQ=255)

                                                                      cGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATGGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGc                                                                                                                                                                     <  1:2014358/238‑1 (MQ=255)

                                                                        tGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTc                                                                                                                                                                                            <  1:550763/214‑1 (MQ=255)

                                                                            aCGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACCC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGa                                                                                                                                                              <  1:1721840/239‑1 (MQ=255)

                                                                                cggcTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCCCTGGCC‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAA‑TCCCGTTTTCGCACCTTGCGCCGCCATAAAAACCCATTCGGAGCGGTGGAGGAGGTGGGTGTTTTCGCGATG‑ACCGAACCGGAGcgggctggagtt                                                                                                                                                          >  2:2174265/1‑228 (MQ=255)

                                                                                            cacGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGCACAGGGGCTGCGTGAAGTCAGCAAcccctg                                                                                                                                               >  2:20041/1‑235 (MQ=255)

                                                                                                cTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGAACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTAGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTgcg                                                                                                                                                                                                                                       <  2:519817/147‑1 (MQ=255)

                                                                                                 ttCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAAt                                                                                                                                         >  2:513698/1‑239 (MQ=255)

                                                                                                 ttCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAAt                                                                                                                                         >  1:1101967/1‑239 (MQ=255)

                                                                                                 ttCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAAt                                                                                                                                         >  2:154409/1‑239 (MQ=255)

                                                                                                              gcgGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGTTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTcg                                                                                                                             <  1:968823/238‑1 (MQ=255)

                                                                                                                            gTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                                                                               >  1:173188/1‑238 (MQ=255)

                                                                                                                            gTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGCGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGa                                                                                                               >  1:1393730/1‑238 (MQ=255)

                                                                                                                            gTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGGGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGGGAATGAGCCGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGGCAGCCACCCCTCGGCAATTTTCTTACATCccggcgcggcaatgc                                                                                                               >  2:2130790/1‑229 (MQ=255)

                                                                                                                              cATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGAcc                                                                                                             <  1:154409/238‑1 (MQ=255)

                                                                                                                                     gggCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGCGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCCCACCTTGCGCAGCCATAAAAACCGCTTCGGCCCGGTGAATGAACTGGGCGTCTTCGCGATG‑ACCGAACCGGGGCTGCGTGAAGCTAGCAACCCTTTGGCAATTTTCTTAAGTCGCGGCGTTGAGATGGCCTCCGGtt                                                                                                      >  2:1509499/1‑237 (MQ=255)

                                                                                                                                      ggCACGTCACCAAAGAGGGTTCGGTGGGT‑GGCCCGAAAGTGCTGGAACAC‑TTTATCGACTGTTCGGTGCTTTGGGATGGCGAGGCCGAC‑TCCCGTTTTCGCACTTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAg                                                                                                     <  2:1777743/238‑1 (MQ=255)

                                                                                                                                       gCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGc                                                                                                    >  1:1300950/1‑238 (MQ=255)

                                                                                                                                       gCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGc                                                                                                    >  1:645080/1‑238 (MQ=255)

                                                                                                                                       gCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGc                                                                                                    >  1:1712334/1‑238 (MQ=255)

                                                                                                                                         acttaccaaaaGATGGTTGGTGGGCG‑GGCCGGAAAGTGCTGAAACAT‑TGTATCGACTGTTCGGTGTTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTc                                                                                                  <  2:2214164/230‑1 (MQ=255)

                                                                                                                                         acttAACCAAAGATGGTGCGCTGGCT‑GGCACGAAAGTGCTGGAACAT‑GTTAGCGCCGTTTGGATGCTTTTGGATGGCGTGGCTGAC‑TCCCGTTTCCGCCCCTTTGGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCATCCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTc                                                                                                  <  2:1624366/235‑1 (MQ=255)

                                                                                                                                         aCGTACCCAAAGATGGTTCGCGGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑GCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTc                                                                                                  <  1:2115382/238‑1 (MQ=255)

                                                                                                                                             aaCCAAGGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGa                                                                                             <  1:985199/239‑1 (MQ=255)

                                                                                                                                               ccAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAtg                                                                                           <  1:1250193/239‑1 (MQ=255)

                                                                                                                                               accaaGATTGTGCGCTGGCTGGGCCGGAAAGTCCT‑GAACGCTTGTTTCGGTTGTTAGGTGTTTGTGGATGGGGAGGCCGAC‑TCCGGTTTCCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAt                                                                                            <  2:1901161/236‑1 (MQ=255)

                                                                                                                                                  aaGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGCCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAtggtg                                                                                        >  1:99091/1‑239 (MQ=255)

                                                                                                                                                      tGGTTCGCTGTCG‑GGCCCGAAAGTGCTGAACCAC‑GGTTTCGACTGTTGGGGGCTTTTGGAGGGCGAGGCCGAC‑GCCCGTTTTCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTAt                                                                                    <  2:1712334/239‑1 (MQ=255)

                                                                                                                                                       ggTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACCC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGGTGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTAt                                                                                    <  1:1512848/238‑1 (MQ=255)

                                                                                                                                                       ggTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCaa                                                                                                                                          <  1:415553/184‑1 (MQ=255)

                                                                                                                                                        gTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATgg                                                                                  >  1:193537/1‑239 (MQ=255)

                                                                                                                                                         ttCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATACAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATggg                                                                                 >  1:1069739/1‑239 (MQ=255)

                                                                                                                                                         ttCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATggg                                                                                 >  1:331679/1‑239 (MQ=255)

                                                                                                                                                          tCGCTGGTT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGCC‑TCCCGTTTTCCCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATggg                                                                                 <  1:1486406/238‑1 (MQ=255)

                                                                                                                                                             ctggct‑ggCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTc                                                                                                  <  1:694282/218‑1 (MQ=255)

                                                                                                                                                               gggtgggcCCGAAAGTGCGGTACCCC‑TGTATCGCCTTTTCGTTGCTTTTGGAGGGCGTTGCCGCC‑TCCGGTTTTGCCCCGTGGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGggaagg                                                                             <  2:886901/233‑1 (MQ=255)

                                                                                                                                                                gct‑ggcCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGTACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCgg                                                                                                        >  2:1381150/1‑209 (MQ=255)

                                                                                                                                                                  tgggcCCGAACGGGCTTGAACAT‑TTTTGCGACTGTTGGGTGTTTTGGGATGGCGTTCCCGAC‑TCCGGTTTTCGCCTCGTGCCCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAGCCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAAc                                                                          <  2:1682400/236‑1 (MQ=255)

                                                                                                                                                                  t‑ggcCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAAcgc                                                                        >  1:1267097/1‑239 (MQ=255)

                                                                                                                                                                    ggcCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAAcgcg                                                                       >  1:1164926/1‑239 (MQ=255)

                                                                                                                                                                      cccGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGt                                                                      >  1:2172169/1‑238 (MQ=255)

                                                                                                                                                                         gAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCAc                                                                  <  1:113697/239‑1 (MQ=255)

                                                                                                                                                                                        c‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCtggt                                                           >  2:725863/1‑231 (MQ=255)

                                                                                                                                                                                        c‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTc                                                   >  1:1631412/1‑239 (MQ=255)

                                                                                                                                                                                          tGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGaa                                                                                                                                                             >  2:328531/1‑132 (MQ=255)

                                                                                                                                                                                           gTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAg                                                 >  1:2211997/1‑239 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAtgg                                                                                          <  1:676419/189‑1 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGAtgg                                                                                          >  2:676419/1‑189 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTc                                        >  2:141681/1‑239 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTc                                        >  1:1150168/1‑239 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTc                                        >  1:2066893/1‑239 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTc                                        >  1:1997264/1‑239 (MQ=255)

                                                                                                                                                                                                    gTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTc                                        >  2:262957/1‑239 (MQ=255)

                                                                                                                                                                                                      tCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATACAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGa                                      >  1:2217468/1‑239 (MQ=255)

                                                                                                                                                                                                      tCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGa                                      >  1:957131/1‑239 (MQ=255)

                                                                                                                                                                                                       cGGTGGTTTTGGATGCGGATGCCGCT‑TCCCGTTTGCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGAt                                     <  1:1819870/239‑1 (MQ=255)

                                                                                                                                                                                                       cGGTGCTTTTAGATTGCGATGCCCAC‑TCCGTTTTTCGCGCAGTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGAt                                     <  2:982052/239‑1 (MQ=255)

                                                                                                                                                                                                          tGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCa                                   >  2:1804695/1‑238 (MQ=255)

                                                                                                                                                                                                               ttGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATggtggt                                                                                      >  2:777540/1‑182 (MQ=255)

                                                                                                                                                                                                               ttGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATggtggt                                                                                      <  1:777540/182‑1 (MQ=255)

                                                                                                                                                                                                               ttGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCCGGTCGAACaaccgaa                             >  2:2205346/1‑233 (MQ=255)

                                                                                                                                                                                                                    tGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCgg                                                                                                        <  1:899291/159‑1 (MQ=255)

                                                                                                                                                                                                                      gCGATGCCGAC‑TCCCGCTTTCGCACCTTGCGCAGCCATAAAAACCGCTCCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCg                      >  1:724992/1‑239 (MQ=255)

                                                                                                                                                                                                                       ccaTGCCGAC‑TACCGTTTTCGCACCTTGCGCAGCCTTAAAAACCGCCTCGGCGCGGGGAATGAGCTGGGCGGCCTCGCGATG‑ACCGAACAGGTGCTGCGTGAAGTCAGCAACCCTTCGGCCATTTTCTTAAGTCGCGGCGATGAAGTGACCCCCGGTAGCTCAGTGATGGTGGTCTGGGAAGGAACGCGTCCACTGCTGGTCGAGATCGTGGAGCTGGTCGATCAGTCGatgctgccca                     >  2:489434/3‑235 (MQ=255)

                                                                                                                                                                                                                                  tCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCAcgcc            >  1:321652/1‑238 (MQ=255)

                                                                                                                                                                                                                                    ccGTTTTCGACCTTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCcgcg         <  1:120872/239‑1 (MQ=255)

                                                                                                                                                                                                                                           cGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGt  <  1:2201556/239‑1 (MQ=255)

GGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCT‑GGCCCGAAAGTGCTGGAACAC‑TGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAC‑TCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATG‑ACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGT  >  NC_007779/4631113‑4631583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`