BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	564,475	A→T	7.4%	S243T (TCA→ACA)	Y75_RS02750 ←	site‑specific integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	564,475	0	A	T	7.4%	428.1 / 6.1	152	S243T (TCA→ACA)	Y75_RS02750	site‑specific integrase
Reads supporting (aligned to +/- strand): ref base A (67/68); new base T (10/2); total (81/71)
Fisher's exact test for biased strand distribution p-value = 3.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGCCACTTC  >  NC_007779/564238‑564710

ttGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACTACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGAt                                                                                                                                                                                                                                              <  2:326357/239‑1 (MQ=255)

ttGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGAt                                                                                                                                                                                                                                              <  1:2113857/239‑1 (MQ=255)

ttGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGAt                                                                                                                                                                                                                                              <  1:921296/239‑1 (MQ=255)

     cAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGct                                                                                                                                                                                                                                         <  1:121009/239‑1 (MQ=255)

     cAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGct                                                                                                                                                                                                                                         <  1:1984579/239‑1 (MQ=255)

     cAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGct                                                                                                                                                                                                                                         <  1:775529/239‑1 (MQ=255)

        gtgtgtCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCAGTCAGCGCC‑ACACCCATGGCCGGGTTTTGTTTtctc                                                                                                                                                                                                                                        >  2:15678/1‑237 (MQ=255)

               tGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCTGTTTGATTTGCTCTCTTCTGGa                                                                                                                                                                                                                               <  1:877342/239‑1 (MQ=255)

               tGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGa                                                                                                                                                                                                                               <  2:1166998/239‑1 (MQ=255)

                  ggTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTCTCTTTCTCCGCC‑ACACCAAACGCGCGGGTTGTTTTTCTCtcttcctggtt                                                                                                                                                                                                                             >  2:249458/1‑232 (MQ=255)

                   gTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGAATc                                                                                                                                                                                                                            >  1:1390950/1‑238 (MQ=255)

                       tGGAAACGGTAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACACACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTACTATTCAGCCCT‑ACGCTAATGCCACGGTTTTATTTGCTGTCCTCTTGATTCACCCa                                                                                                                                                                                                                       >  2:1406905/1‑239 (MQ=255)

                           aaCGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTGTGATTTGCGCTCTTCTGGATTCACCCAGGc                                                                                                                                                                                                                    <  1:1419509/238‑1 (MQ=255)

                                          ttCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCTGTCAGCGCC‑ACACCAATTGCGCGGTTTGATTTGCCCTCTTCTCGATTCACCCAGGCAACCCGCCGCCGCGTg                                                                                                                                                                                                    >  2:633098/1‑239 (MQ=255)

                                           gccTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGt                                                                                                                                                                                                   <  2:1405452/238‑1 (MQ=255)

                                                          aGCTGATAGCCATGATGTCTTGCTGTCGATGCACATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGGGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGTTTCTCCCACGCAACCCCTCGCTGCACTTCGGTTTGT‑TTCCAtt                                                                                                                                                                                   >  1:1892773/1‑239 (MQ=255)

                                                           tcTGATAGCCATGATTTCTTGCTGTCGCTGTCCATCTTCCTTACCGCAGGCGTTGATTTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACCCCCATTTGTGATGCTTGCCTATTTGATCGCGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTAGATTCACCCAAGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTc                                                                                                                                                                                  <  2:480623/238‑1 (MQ=255)

                                                            cTGATAGCCATGATGTCCTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTAGTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGGAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTc                                                                                                                                                                                  <  1:1062869/238‑1 (MQ=255)

                                                             tgttAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTc                                                                                                                                                                                  <  2:1107920/234‑1 (MQ=255)

                                                               aTAGCCCTGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTTTTTTTCAGCGCC‑CACCCAATTGGGCGGTTTGTTTTTCTCTCTTTTGGCTTCACCCAGGCACCTCGTCGCCGCATGTCGCTTTGC‑TTCCCTTcatta                                                                                                                                                                              >  2:1218165/1‑235 (MQ=255)

                                                               aTAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTCTTGTTCAACGCC‑ACACCCAAGGCGCCGTTTTTGTTTCCCTCTTTCGGAATCACCCCAGCAACATGTCCCCGTCTGTCGATTTTT‑TTCCCtttccta                                                                                                                                                                              >  2:1486161/1‑234 (MQ=255)

                                                                      tgatgTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTCTCGTTCAGCGCC‑ACACCCAAGGCGCGGTTTGATTTGGTCTCTTCTGGATTTGCCCACGCAAATCGTCTCTGCAGCTCGCTTTTT‑TGCCATTTCCTATTTatt                                                                                                                                                                        >  2:1062869/1‑237 (MQ=255)

                                                                              tGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGAc                                                                                                                                                               <  1:954189/239‑1 (MQ=255)

                                                                                    cGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTCACCTTTAGCGCC‑ACACCAAAGGCGCGGTTTTATTTGTTCTCTTTTGGATTCACCCAGGGAAATCGGCGCTGCATGTCGCTTTGTGTGCC‑TTCCAGTGTTATGATGTTCGCCttttt                                                                                                                                                          >  2:2100114/1‑236 (MQ=255)

                                                                                     gATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACTCTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCa                                                                                                                                                        <  2:1879802/239‑1 (MQ=255)

                                                                                     gATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGACCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑CCACCAATGGCGCGGTTTGTTTTGTTCTTTTCTGGGTTCCCCCAGTCAACTCCTCGCGCCATGTCGATTTTT‑TTCCCTTTCCGCTTTAAGGTCTTTGGCTTtctc                                                                                                                                                         >  2:2170968/1‑238 (MQ=255)

                                                                                     caTGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGACCCCGCAACACTTTACAGGCGGAATCGGTCACAGCC‑ACCCCAAACGCGCGCGTTGATTTTGTCTCTTCTGGCTTCCCCCCGGCCACCCGTCGCTGCCTCTCCATTTGT‑TTCCCTTCCAGATCTATTATGTTCGTCTTtccc                                                                                                                                                         >  2:1913072/2‑236 (MQ=255)

                                                                                             tCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATTGCCCGGGTTGCTTTGCTCTCTTCTGGATTCACCCCGGCAACTCGTCCCTGCATGTCGATTTTT‑TGCCATTCCAGATTTTTGATGTTCTACTTTTTCCGACCAGtt                                                                                                                                                >  1:347278/1‑239 (MQ=255)

                                                                                                      ccGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGACTTGGTATGTACAAACCCCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGAGTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCa                                                                                                                                       <  2:779697/239‑1 (MQ=255)

                                                                                                        gCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTCTCGTTCAGCGCC‑ACACCAATGGCGCGGGTTGATTTGCTCTCTTCTGGAATCACCCAAGCAACTCGTCGCTTCATGTCGATTTGT‑TGCCATTCCAGATTTATGAAGTTCGACTTTCTCAGACCAGTTGCCAGCGCaaa                                                                                                                                     >  1:2211688/1‑239 (MQ=255)

                                                                                                         cAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTTT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCaa                                                                                                                                      >  1:432482/1‑237 (MQ=255)

                                                                                                          aGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTTCTCTCTTCTTGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTTTGATGTTCGACTTTTTCAGACCAGTTGCCAGCGCAAACt                                                                                                                                   >  1:1402716/1‑239 (MQ=255)

                                                                                                          aGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGGATCGTTCAGCGCC‑CCACCAATGGCGCGGTTTTATTTGCTCTCTTCTGGATTCCCCCAGGCAACTCGTCGCCGCATGTCGATTTTT‑TGCCATTCCAGATTTCTGATGTTCGACTTTTTCAGACCAGTTGCCAGCGCAAACt                                                                                                                                   >  1:842438/1‑239 (MQ=255)

                                                                                                                   tGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACa                                                                                                                          <  1:435172/238‑1 (MQ=255)

                                                                                                                          tCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGTC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTCTGATGTTCGACTTTCTCAGACCAGTTGCCAGCCCAAACTTGACG‑ACAGAttttcg                                                                                                                  >  1:516839/1‑236 (MQ=255)

                                                                                                                          tCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTTGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAg                                                                                                                  >  1:67597/1‑239 (MQ=255)

                                                                                                                          tCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAg                                                                                                                  <  1:1242241/239‑1 (MQ=255)

                                                                                                                          tCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGACTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCCACCCC‑ACACCAATGGCCCGGTTTGCTTTGCTCTCTTCTGGATTCCCCCCAACAACACGCACCTGCCTGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTTGACTTTCTCAGTCCCAGTGCCAGCGCAACCTTGACC‑ACAGAttttcg                                                                                                                  >  2:1307157/1‑236 (MQ=255)

                                                                                                                             gCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCACGCAACTCCTCCCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTgg                                                                                                               >  2:613493/1‑239 (MQ=255)

                                                                                                                              cTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTTATTTGCTCTCTTCTGGCTTCACCCACGCCACTCGTCGCTGCATGTCGATTTGT‑TTTCCTTCCAGATTTATGATGTTCGCCTTTCTCAGACCAGTTGCCAGCGCAAAATTTATG‑ACCGATTTCTGTggg                                                                                                              >  1:1151803/1‑238 (MQ=255)

                                                                                                                               tCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCa                                                                                                                   <  2:422799/233‑1 (MQ=255)

                                                                                                                               tCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGt                                                                                                              <  1:317895/238‑1 (MQ=255)

                                                                                                                                 gCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCg                                                                                                           <  1:477404/239‑1 (MQ=255)

                                                                                                                                    tggtctccTTGGTATGTACAAACCCCCATTTGTGGTGCTTGCCTATTTGATCACGCAACACTTTTCAGGCGTTATCGTTCAGCGCC‑ACGCCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCgggg                                                                                                        <  2:1390950/233‑1 (MQ=255)

                                                                                                                                    tAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACGCTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCgggg                                                                                                        <  1:890318/239‑1 (MQ=255)

                                                                                                                                    tAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTTGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCgggg                                                                                                        <  2:67597/239‑1 (MQ=255)

                                                                                                                                      gccgccTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGTCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCa                                                                                                      <  2:147218/239‑1 (MQ=255)

                                                                                                                                        cgccTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCa                                                                                                      <  1:241752/237‑1 (MQ=255)

                                                                                                                                           cTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATTTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACtca                                                                                                  >  1:733774/1‑238 (MQ=255)

                                                                                                                                           cTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAAAGGCGCGGGTTGATTTGCTCTCTTTTGGATTCACCCCGGCAACCCGTCGCTGCATGTCGATTTTT‑TGCCCTTTCAGATTTATGATTTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGGTTTTCGTGGTTCGGGGCACtcc                                                                                                  >  2:851454/1‑237 (MQ=255)

                                                                                                                                             tGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTTCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCCCtcatca                                                                                               >  1:1453167/1‑239 (MQ=255)

                                                                                                                                               gTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGt                                                                                                                                                                    <  1:1823549/169‑1 (MQ=255)

                                                                                                                                                tATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAAt                                                                                             <  1:947522/238‑1 (MQ=255)

                                                                                                                                                 aTGTACAAACACCCATTTGTTATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGCCCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATa                                                                                            <  1:73346/238‑1 (MQ=255)

                                                                                                                                                       aaaCACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCCGCGCC‑ACACCAATGGCGCCGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACCCCTCGCTGCATGTTGATTTTT‑TTCCATTTCAGATTTATGTTGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTTAAG‑ACAGATTTTCGTGGTTCGGGGCACTCCTCAATAAGGTGtt                                                                                     >  1:1158306/1‑239 (MQ=255)

                                                                                                                                                         acacCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATAGTTTAGGGCC‑AAACCCATGGCGCGGTTTTATTTGCTCTCTTCTGGATTCACCCACGCAACTCGTCTCTTCATGTCGATTTGT‑TGCCCTTCTCGCATTATGATGTTCTACTTTTTCCGCCCCTTTGCCAGCGCCAACTTGACGCACAAATTT‑AATTGTTCGGGGCCCTCCTCAATTAGGCCtttt                                                                                   >  2:465695/1‑239 (MQ=255)

                                                                                                                                                              cATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATTGCGCGGTTTGATTTGCTCTCTTCCTGATTCACCCAGGCAACTCCTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTTTTAGACCAGCTGCCCTCGCCAACTTGTCG‑ACCGATTTCATTGGTTTGGGGCACTCCTTCATAAGGCGTTTTGCtt                                                                               >  1:1008614/1‑238 (MQ=255)

                                                                                                                                                              cATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGAATTGTTCAGCTCCAACACAAAT‑AAACGGTTTTATTTTCTCTTTTTTGGATTATCCCAAGCAACTCGGCCCGTCCCCTCGATTTTT‑TTTCACTACTAATTTTTGGTTGTCCACTTTTCCAGGTCCGTTTACCGGCCTAACTTTAAT‑ACACATTTGCGTCGTTCGGGGCACTCCTCAATAAAGCGgttttttt                                                                               >  2:1197009/1‑234 (MQ=255)

                                                                                                                                                               aTTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACATATTTCAGTGGTTCGGGGCACTCATCAATa                                                                                            <  1:31999/224‑1 (MQ=255)

                                                                                                                                                               aTTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTtcc                                                                             <  1:249458/239‑1 (MQ=255)

                                                                                                                                                                tttGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACtc                                                                                                   <  2:390826/216‑1 (MQ=255)

                                                                                                                                                                tttGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACAACAATGGCGCGGTTTGATTTGCTCTCTTCTGGGTTCACCCAGGCAACTCGTCGCTGCATGTCGATTTTT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACtc                                                                                                   >  1:390826/1‑216 (MQ=255)

                                                                                                                                                                       tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATTATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCtttt                                                                      >  1:1224770/1‑238 (MQ=255)

                                                                                                                                                                       tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTc                                                                     <  2:219524/239‑1 (MQ=255)

                                                                                                                                                                       tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACAACAATGGCGCGGGTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTTCCGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAAATTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCtttt                                                                      >  1:1612095/1‑238 (MQ=255)

                                                                                                                                                                       tGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGAGCC‑ACACCAATGGCCCGGTTTGCATTGCTCCCTTCTTGCTTCACCCAAGCAAATCGCAGCTGCATGTCGCTTTTT‑TGCCATTCCAGATTTATTATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACATTCAG‑ACAGATTTCCGTGGTTCGGGGCACCCCTCAATAAGGCGGTTTGCTTCCTCCtttt                                                                      >  1:656859/1‑238 (MQ=255)

                                                                                                                                                                         cTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCCATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCCGGCAACTCGTCGCTGCATGTCGATTTGG‑TTCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGGTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCa                                                                   >  1:1418482/1‑239 (MQ=255)

                                                                                                                                                                         cTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCTACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCa                                                                   >  1:330966/1‑239 (MQ=255)

                                                                                                                                                                         cTTGCCTATTTGATCACGCAACACTTTACAGGCGGAATCGTTCAGCGCC‑ACACCAATTGCGCGGGTTGATTTGCTCTCTTCTGGATTCCCCCCGGCAACTCGTCGCCGCCTTGCGTTTTGT‑TGCCCTTCCACATTTATTATGTTCGACTTTCTCAGACCCGTTTGCCGCGCCAACTTGACG‑ACAGATTTCAGTGGTTCGGGGACCTCCTCAATAAGGCGTTTTGCTTCCTCCTTTTcc                                                                    >  2:1620466/1‑238 (MQ=255)

                                                                                                                                                                          ttGCCTATTTGATCTCGCAACGCTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGATTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCa                                                                   <  1:1981776/238‑1 (MQ=255)

                                                                                                                                                                           tGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGt                                                                                                                                                                    >  1:114721/1‑141 (MQ=255)

                                                                                                                                                                           tGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGt                                                                                                                                                                    <  2:114721/141‑1 (MQ=255)

                                                                                                                                                                              cTATTTGATCACGCAACACTTTACAGGCGGGATCGTTTTGCGCC‑CCACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGTTTCACCCACGCAACCCGCCGCCGCATTTCGTTTTGT‑TGCCATTCCAGATTTATGATGTTCGACCTTCTCACACTAGTTCTCCGCGCCAACATCACT‑ACAGATTTTCGTGGTTTGGGGCTCTCATTAATAAAGCGTTTTGTTTCCTCCTTTTTCCGccc                                                               >  2:1981776/1‑237 (MQ=255)

                                                                                                                                                                               tctttGGTCCCGCAACACTGTCCAGGGGGTATCATTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTAGATTCACCCAGGCAACTCGTCAGTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGGCCAGTTGCCAGCGCAGACTTGTCT‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGTCGTTTTGCTTCCTCCTTTTCCAGCCATc                                                             <  2:569025/237‑1 (MQ=255)

                                                                                                                                                                                 tttGATCACGCAACACTTTACAGGCGGTATCGTTCCGCGCC‑ACACCAATGGCGCGGGTTTATTTTCTCTCTTCTTGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTTT‑TGCCCTTCCAGATTTATGAATTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTTACG‑ACAGATTTCAGTGGTTCGGGGCCCTCATCAATAAGGCGTTTTGCTTCCTCCTTTTTCAGCCacttg                                                           >  2:340867/1‑235 (MQ=255)

                                                                                                                                                                                      tCACGCAACACTTTACAGGCGGTATCGTTCCGCGCC‑ACACCAATGGCGCGGTTTTTTTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTTACG‑ACAGATTTCATTGGTTCGGGGGACTCCTCAATAAGGCGCTTTGCTTCCTCCTTTTCCAGCCATCTGAccc                                                       >  2:191408/1‑236 (MQ=255)

                                                                                                                                                                                      tCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGGTTTATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCCGCATGTCGATTTTT‑TGCCATTTCAGATTTATGATTTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCTGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTc                                                       >  2:1170438/1‑238 (MQ=255)

                                                                                                                                                                                         cGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCCTTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTTACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCtt                                                   >  1:383443/1‑239 (MQ=255)

                                                                                                                                                                                            aaCACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGGTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTTT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCCTAATCGCTTGtt                                                >  1:1692534/1‑239 (MQ=255)

                                                                                                                                                                                               acTTTACAGGCGGTATCGGTCAGCGAC‑ACACCAATAGCGCGGGTTTATTTGCTCTCTTCTTGGTTCACCCCGGCAAACCGTCGCTGCAAGTGGATTTGT‑TGCCATTCCCGATTTATGATGTTCGACTTTCTCAGGCCAGTTGCCAGCGCAAACCTTACC‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCAGTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTtttt                                                >  2:1361810/1‑236 (MQ=255)

                                                                                                                                                                                                 tttACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTg                                            >  1:1094477/1‑238 (MQ=255)

                                                                                                                                                                                                   tACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACt                                                                                                    <  2:179053/180‑1 (MQ=255)

                                                                                                                                                                                                        gCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTTTTTTTCTCTCTTCTTGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TTCCATTCCCGATTTTTTATGTTCGACTTTCTCAGACCCGGTGCCAGCCCAAACTTTACG‑AAAGATTTTCGTGGTTCGGGGCACTCCTCAATAAGGCGTTTTTCTTCCCCCTTTTTCAGCCCTCTTACCCGCTTTTTTTTGGCCCCTgg                                    >  2:1546157/1‑239 (MQ=255)

                                                                                                                                                                                                        gCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTgg                                    >  1:1731584/1‑239 (MQ=255)

                                                                                                                                                                                                        gCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGGTTTATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTgg                                    >  1:1616792/1‑239 (MQ=255)

                                                                                                                                                                                                        gCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGATTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATTTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTTACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTgg                                    >  1:1960562/1‑239 (MQ=255)

                                                                                                                                                                                                         cGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCt                                                    <  1:1892489/222‑1 (MQ=255)

                                                                                                                                                                                                                  tCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTtgatg                          >  2:778377/1‑239 (MQ=255)

                                                                                                                                                                                                                      cgcC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAg                      >  1:1078638/1‑239 (MQ=255)

                                                                                                                                                                                                                       gcC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCGATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAg                      <  1:1803942/238‑1 (MQ=255)

                                                                                                                                                                                                                            cacCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTc                                                                     <  2:1929417/187‑1 (MQ=255)

                                                                                                                                                                                                                            cacCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTc                                                                     >  1:1929417/1‑187 (MQ=255)

                                                                                                                                                                                                                                 aTGGCGCGGTTTGTTTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCttttt             >  2:1657232/1‑238 (MQ=255)

                                                                                                                                                                                                                                 aTGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCgggg                                                                                                        <  2:1846977/147‑1 (MQ=255)

                                                                                                                                                                                                                                      gcgGTTTGTTTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCCCTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGCCCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGc        <  2:1151803/238‑1 (MQ=255)

                                                                                                                                                                                                                                      gcgGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGcc       >  1:2060502/1‑239 (MQ=255)

                                                                                                                                                                                                                                       cgGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGCCCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGcc       >  2:1325374/1‑238 (MQ=255)

                                                                                                                                                                                                                                         gTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGCCACt    >  1:1894118/1‑239 (MQ=255)

                                                                                                                                                                                                                                         gTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGCCACt    >  1:2214067/1‑239 (MQ=255)

                                                                                                                                                                                                                                           ttGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGCCACTTc  <  1:1546157/239‑1 (MQ=255)

TTGCCCAGGTGTGTCTGAGGTCATGGAAACGGAAATCTTCAATTCCTGCACGACGACAAGCTGATAGCCATGATGTCTTGCTGTCGATGCGCATCTTCCTGACCGCAGGCGTTGATGTTCCATCTGCTCGCTTAGCCGCCTTGGTATGTACAAACACCCATTTGTGATGCTTGCCTATTTGATCACGCAACACTTTACAGGCGGTATCGTTCAGCGCC‑ACACCAATGGCGCGGTTTGATTTGCTCTCTTCTGGATTCACCCAGGCAACTCGTCGCTGCATGTCGATTTGT‑TGCCATTCCAGATTTATGATGTTCGACTTTCTCAGACCAGTTGCCAGCGCAAACTTGACG‑ACAGATTTCAGTGGTTCGGGGCACTCATCAATAAGGCGTTTTGCTTCCTCCTTTTCCAGCCATCTGACTCGCTTGTTTCTGACCGCTGGTATCTTGATGACAGGCGCTTTTTCCAGCCACTTC  >  NC_007779/564238‑564710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`