BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	667,452	G→T	6.8%	A396A (GCC→GCA)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	667,452	0	G	T	6.8%	502.6 / 12.4	163	A396A (GCC→GCA)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand): ref base G (82/68); new base T (10/2); total (93/70)
Fisher's exact test for biased strand distribution p-value = 7.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 167 total aligned reads displayed.

GTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACC  >  NC_007779/667215‑667689

gTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg                                                                                                                                                                                                                                                 <  1:1674195/239‑1 (MQ=255)

 tCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg                                                                                                                                                                                                                                                 >  2:155647/1‑238 (MQ=255)

 tCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg                                                                                                                                                                                                                                                 >  1:836701/1‑238 (MQ=255)

    ttAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc                                                                                                                                                                                                                                             <  1:1556743/239‑1 (MQ=255)

       aTCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg                                                                                                                                                                                                                                          <  1:155647/239‑1 (MQ=255)

        tCAGGATCATCAGTGCCTTCCTCAACTGGATTGGGGTCCCTGTCCAGTACCCCTGACCGATACCAACCGGAATGGTTTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa                                                                                                                                                                                                                                         <  2:964014/239‑1 (MQ=255)

        tCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc                                                                                                                                                                                                                                             >  1:1071667/1‑235 (MQ=255)

          aGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATa                                                                                                                                                                                                                                       >  1:1694619/1‑239 (MQ=255)

             atcatcAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag                                                                                                                                                                                                                                      <  2:1057830/237‑1 (MQ=255)

              tcatcaGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaag                                                                                                                                                                                                                                   <  2:905150/239‑1 (MQ=255)

                               aTCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GAt                                                                                                                                                                                                                   >  1:1948594/1‑237 (MQ=255)

                                 cTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGACCACCTGATAGCAGAAGGTATCCGCA‑GCttct                                                                                                                                                                                                                >  2:78317/1‑238 (MQ=255)

                                  tGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGCCTTATAGATGAAGAAATCAGCA‑GAttctt                                                                                                                                                                                                               >  1:1049779/1‑238 (MQ=255)

                                     aTTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCa                                                                                                                                                                                                            <  2:1515535/238‑1 (MQ=255)

                                      ttGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGGATGGTGTCCCCCTGATACCACGTTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCTCCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAg                                                                                                                                                                                                           <  2:2161874/238‑1 (MQ=255)

                                      ttGGGGTCGCTGTCCAGTAACCCTGACCGAGACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCACTCGCGGGTTGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAAGTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCTTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGc                                                                                                                                                                                                          <  2:1880584/239‑1 (MQ=255)

                                         gggTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑a                                                                                                                                                                                                       >  1:1453857/1‑238 (MQ=255)

                                         gggTCACTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGCATTTACCCCTCCATTCGCCGAGGCGGTCGATCCCCATTCCGTAGGCCGCCTGTTAGGAGCCGGTATCCGAA‑GCTTCTCCCACCG‑a                                                                                                                                                                                                       >  2:451027/1‑238 (MQ=255)

                                             ccgtGTCCAGTACCCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCGTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTtg                                                                                                                                                                                                  <  2:834174/236‑1 (MQ=255)

                                              gCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTtgt                                                                                                                                                                                                 >  2:177941/1‑239 (MQ=255)

                                                tGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATGCCACGGTTTTTTAAAGCGTTTCTGTTGCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTtgtg                                                                                                                                                                                                <  2:1265749/238‑1 (MQ=255)

                                                   ccAGTAACCCTGACCGATACCAACCGGAATGGTGCCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCAAGCG‑ATCTTGTGACAt                                                                                                                                                                                            >  1:2173929/1‑239 (MQ=255)

                                                          cccTGACCGATACCAACCGGAATGGTGTCACCCTGATACAACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGAATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGAc                                                                                                                                                                                      >  1:1351271/1‑238 (MQ=255)

                                                            cTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATCTCGTATGCCACCTTCTACCTGAAGGAATCAGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGActa                                                                                                                                                                                    >  1:1155940/1‑236 (MQ=255)

                                                            cTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGc                                                                                                                                                                                    >  1:1756481/1‑238 (MQ=255)

                                                            cTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGc                                                                                                                                                                                    >  1:1034231/1‑238 (MQ=255)

                                                              gACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCg                                                                                                                                                                                 >  1:2144294/1‑239 (MQ=255)

                                                               aCCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaa                                                                                                                                                                                                                                    >  2:1489013/1‑189 (MQ=255)

                                                                   aTACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGAt                                                                                                                                                                                                                                        >  2:1604517/1‑181 (MQ=255)

                                                                   aTACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGAt                                                                                                                                                                                                                                        <  1:1604517/181‑1 (MQ=255)

                                                                    tACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGcc                                                                                                                                                                            >  1:756208/1‑238 (MQ=255)

                                                                       ctgccGGAATGGTGGCACCCTGATACCCCGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAAGCATGTTTCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCAt                                                                                                                                                                        <  2:28992/236‑1 (MQ=255)

                                                                         aCCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTTCTACAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGGGACATTCAGACGCCCGTGGCCCCAttt                                                                                                                                                                      >  1:1148988/1‑239 (MQ=255)

                                                                               aTGGTGTCCCCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCa                                                                                                                                                                <  1:377842/239‑1 (MQ=255)

                                                                                   agtCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGTCTTATAATAGAAAGAATCAGCA‑AAGTCTTCCAGCA‑CTCTTGCGGCATTCAGACGCCCACAG‑CCCATTTTGTTCAGTc                                                                                                                                                             >  1:2214832/2‑237 (MQ=255)

                                                                                      cACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGa                                                                                                                                                         <  2:756208/239‑1 (MQ=255)

                                                                                       aCCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGAt                                                                                                                                                        >  1:246088/1‑239 (MQ=255)

                                                                                             aTACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACACA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACgct                                                                                                                                                  >  1:2083969/1‑237 (MQ=255)

                                                                                             aTACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGTCTGCTGCTTGAAGAAAAACGAA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGTCCGTGGCCCCATTTTTTCCAGTCACGATAACGtt                                                                                                                                                  >  1:141983/1‑239 (MQ=255)

                                                                                             aTACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa                                                                                                                                                                                                                                         <  1:2050684/154‑1 (MQ=255)

                                                                                             aTACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGCTAGATGAAGGAATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGtt                                                                                                                                                  >  1:1097727/1‑239 (MQ=255)

                                                                                             aTACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGtt                                                                                                                                                  >  1:1492589/1‑239 (MQ=255)

                                                                                                 cACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGttttt                                                                                                                                               >  1:1574685/1‑238 (MQ=255)

                                                                                                   cGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGAAGGCCACCTGATAGAAGAAGGTATCCGCA‑GCTTCTTCCACCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAAGGTTTTTcct                                                                                                                                            >  1:656872/1‑238 (MQ=255)

                                                                                                       tttttAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGaa                                                                                                                                          >  1:128405/1‑237 (MQ=255)

                                                                                                        ttttAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGaa                                                                                                                                          >  1:1239175/1‑236 (MQ=255)

                                                                                                          ttAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTgg                                                                                                                                     <  2:1185502/239‑1 (MQ=255)

                                                                                                              atcGTTTTTGTTTCCATTCGCGAGTAGGCATGTTGCCGGAACTTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGt                                                                                                                                 <  2:1512295/237‑1 (MQ=255)

                                                                                                                  tttCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGcc                                                                                                                             >  1:872840/1‑239 (MQ=255)

                                                                                                                        tttCCATTCGCGGTTAGTCATGTTCCCGGATGTTGCGGCCGCCAGGTCGATACCGGGGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCCGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGGTTTTCCGAACCTGGCAGTTGGCACCAGc                                                                                                                       <  2:211547/239‑1 (MQ=255)

                                                                                                                         ttCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCGGGTCGATCCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGc                                                                                                                       <  1:97013/238‑1 (MQ=255)

                                                                                                                             aTTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTggg                                                                                                                  >  1:908916/1‑239 (MQ=255)

                                                                                                                                   gggTAGGCAGGTTTCCGGAACGTTCTTCCGCCAGGTCCGTACCGGTGGAATGATCATAGCCGAATTTACCCAGCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTATGACATTCAGACGCGCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCaaa                                                                                                             <  2:2144294/238‑1 (MQ=255)

                                                                                                                                     gttggCATGTTGCCCGAACGTTTTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTGCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACa                                                                                                           <  2:656872/235‑1 (MQ=255)

                                                                                                                                     cgagtatcgTTACCGGAACGTTCTTCCGCTAGGTCGATACCGGTGTAATGACCATAGCGGAATTTGCCCAGCCAGTCGGAGAGGCGATCGATCCCCATATCGTAGTCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCATCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCGCGATAACGTTTTTCCCAACCTGGCAGTTGCCACCAGCCTGGGTCAAACa                                                                                                           <  2:462763/230‑1 (MQ=255)

                                                                                                                                          cATGTTGCCGGAACGTTCTGCCGCCATGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGcgt                                                                                                       <  2:571401/237‑1 (MQ=255)

                                                                                                                                             gTTGGCGGAACGTTCTTCCGCCAGGTCGAGACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCTCCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTa                                                                                                   <  1:451027/238‑1 (MQ=255)

                                                                                                                                             gTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTAt                                                                                                  >  1:1885827/1‑239 (MQ=255)

                                                                                                                                               tGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCt                                                                                                                     >  1:1263783/1‑218 (MQ=255)

                                                                                                                                                gCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgc                                                                                               >  1:1794579/1‑239 (MQ=255)

                                                                                                                                                  cGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTgcgc                                                                                             <  1:709195/239‑1 (MQ=255)

                                                                                                                                                    gAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGt                                                                                           >  1:1844732/1‑239 (MQ=255)

                                                                                                                                                       cGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGcc                                                                                                                                                                            <  2:2616/155‑1 (MQ=255)

                                                                                                                                                       cGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGcc                                                                                                                                                                            >  1:2616/1‑155 (MQ=255)

                                                                                                                                                                      tCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCt                                                                           <  1:2227051/236‑1 (MQ=255)

                                                                                                                                                                               gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGAATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGCGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACAAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCACGGCGCTAAATGCCGaac                                                                >  2:500765/1‑237 (MQ=255)

                                                                                                                                                                               gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGAACGCCACCTGATAGACGAAGGCATCAGCA‑GAATCATCCAGCG‑ATCTGATGACATTCAGACGCCCGAGACACCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGACAGTTACCACGAGCCTGTGACAGACATCGTCGGATTGCGCGTGAT‑CCACCTGGTCATCAATGcctatc                                                                >  2:355095/1‑234 (MQ=255)

                                                                                                                                                                                 ttAATGACCATACCCGATTTAACCAATCAATTCGGAGAGGCTATCGTTCCCCATGTCTTAGGCCCCCTGATAGAGACAGTTATCCGCT‑GTTTCTTCCAGCT‑TTTTTTTCTCATTCAGACGCCCGTGGCCCCTTTTTTTCCAGTCACGATAACGTTTTTCCGATCCGGGCAGTTGCCACCAGCCGGGGCAAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCATTGCCGAAAcc                                                              <  2:1718202/237‑1 (MQ=255)

                                                                                                                                                                                  tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGTCTTCTGCTTGAAAAAATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGaaagag                                                             >  1:686337/1‑235 (MQ=255)

                                                                                                                                                                                    aTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGcc                                                           >  1:1597341/1‑238 (MQ=255)

                                                                                                                                                                                    aTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGACTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGcc                                                           >  1:195518/1‑238 (MQ=255)

                                                                                                                                                                                      gACCATTACCGAATTTCCCCCTCCATTCGGAGGGTCGATCGTCCCCCATTTCGGAGCCCTCCTTTAAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCCCGATACCGTTTTTCCGACCCTGGCAGTTGCCCCCAGCCTGGGTCAAACAGCGCCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCcaca                                                        <  2:2118274/239‑1 (MQ=255)

                                                                                                                                                                                       aCCATAACCGAATTTACCCATCCATTCGGAAGGGCGATCGTTCCCCATATCGTAGGCCACCTGATAGAGGAGGGTATCCGCA‑GATTCTTCCAGCG‑ATCTGGTGACATTCAGACGCCGGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGACCCTGGCAGTGGCCCCCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CCCCCCGGCGCTCAATGCCGAAACCGCcaca                                                        <  2:1434770/238‑1 (MQ=255)

                                                                                                                                                                                         cATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAg                                                     >  1:2118274/1‑239 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGcca                                                                                                                            <  2:927301/168‑1 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAgg                                                    >  1:1175134/1‑239 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAgg                                                    >  1:936528/1‑239 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAgg                                                    >  1:589342/1‑239 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAgg                                                    >  1:2002420/1‑239 (MQ=255)

                                                                                                                                                                                          aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTAAT‑CACCCCGGCGCTCAATGCCGAAACCg                                                             <  1:171681/230‑1 (MQ=255)

                                                                                                                                                                                                   tttACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTa                                               >  2:1221040/1‑235 (MQ=255)

                                                                                                                                                                                                           tccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGAcgcg                                   >  2:1854194/1‑239 (MQ=255)

                                                                                                                                                                                                             catTCGGTGGGGGGGTCGATCCCATTTCCGAAGGCCACCTGTTA‑AAGAAGGGGACCGGATGATTTTTCCACCT‑AGCTTGTGCCATTCAGACGCCCGGGGCCCCATTTTTTCCATCCACGATAACGTTTTCCCAACCCGGGGATTGGCCCCCAGCCTGGTACAAACAGGGTGGTATTGCGCGTGAC‑CCCCCCGGCGCTAAAGGCCAAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                 <  2:2081952/239‑1 (MQ=255)

                                                                                                                                                                                                               ttCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGCTAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTaa                                              >  1:1837496/1‑224 (MQ=255)

                                                                                                                                                                                                                tCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGACCCTGGCAGTTGCCACCAGc                                                                                                                       <  2:1351366/151‑1 (MQ=255)

                                                                                                                                                                                                                tCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGc                                                                                                                       >  1:1351366/1‑151 (MQ=255)

                                                                                                                                                                                                                    agagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAGaa                          >  1:1305564/1‑239 (MQ=255)

                                                                                                                                                                                                                     ggggCGATCGTTCCCCATTGCGTAGCCCCCCAGATAAAAGAAGTTACCCGCA‑GTTTCTTCCAGCG‑TTTTTGTGACATTCAGACGCCGGGGCCCCCTTTTTTTCCATTCACGATAACGTTTTTCCGACCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAC‑CCCCCCGGCGCTCAAGGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaa                          <  2:1597341/236‑1 (MQ=255)

                                                                                                                                                                                                                        gCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcc                       >  2:30899/1‑238 (MQ=255)

                                                                                                                                                                                                                           atcgatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCggg                                 >  2:676719/1‑225 (MQ=255)

                                                                                                                                                                                                                                tcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg              >  1:522710/1‑239 (MQ=255)

                                                                                                                                                                                                                                 ccccATATTGTGTGCCCCCTACGAAAAGAGGCTGTCCGCG‑AATTCTTCC‑GCGAACCTGGTTACATTCAGACCCCCGTGGCCCCATTTTTCCCAGTAACGATAACGTTTTCCCGAACCTGGCAGTTGCCACAAGCCTGGGCCAAACAGCGGCGTTTTGCGCGTGAT‑CCCCCCGGCGTCAAATGCGGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTgggg             <  2:1151944/239‑1 (MQ=255)

                                                                                                                                                                                                                                   ccATATCGTATGCCACCTGATAGAAGAAAGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGCTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGAGAAACCCCCtgtgtgca            >  1:1127314/1‑236 (MQ=255)

                                                                                                                                                                                                                                    atgatgggcggCCACATATTAGAGGAAGGTTTCGGCG‑TATTCTTCAGGCT‑ATCTTGTAGGTTTCGGCACCCGGTGGCACCTTTTTCCCCAGCGACGATAACGTTTTTCCGAGGCGGTCGGTGGCCACAGGCGGGGGTAAAGCAGGGTGTTTTTGGGCG‑GATGCCCCCGGGCGTGCAATGCCGAAGCCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGgcgc          <  2:1815869/230‑1 (MQ=255)

                                                                                                                                                                                                                                      atggtgaGGCCTCGTGATAGAAGAAGGTATCCGCG‑GATTCTTCCAGCG‑GTTTTGTGACTTTCAGCCGCCCGTGTCCCCATTTTTCCCTGGCACGATACCTTTTTTCCGAACCGGGCATTTGCCCCCAGCTTGGGTCAAACAGCGTCGTATTGCGGGTGAT‑CCCCCCGGCGCTAAATGCCAAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCgg        <  2:1841058/233‑1 (MQ=255)

                                                                                                                                                                                                                                       agcGTAGGCCCCCTGATGGAGGAAGGTATCCGCG‑AGTTCTTCCAGCG‑CTCGTCGGACATCCACAGCCCCGTGTCCCCATTTTTTCCAGTCACCAAACCTTTTTTCCAACCCGGGCAGTTCCCCCCCGCCGGGGTCAAACAGCGTCGTTTTGGGCGTAAC‑CACCCCGGCGCCCAATCCCGAAACCGCCCCATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCgg        <  2:2147475/236‑1 (MQ=255)

                                                                                                                                                                                                                                          gTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTc     >  1:303038/1‑238 (MQ=255)

                                                                                                                                                                                                                                           tAGGCCACCTGCTAGAAGAAGGTATCCGCA‑GATTCCTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGAAGACGCGGGAGGATAAACCCCCCTGGTGGCGCGGTTcc    >  2:566304/1‑237 (MQ=255)

                                                                                                                                                                                                                                           tAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACa                                                                                                           >  1:450604/1‑136 (MQ=255)

                                                                                                                                                                                                                                           tAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCa    >  1:1453390/1‑238 (MQ=255)

GTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGATTGGGGTCGCTGTCCAGTAACCCTGACCGATACCAACCGGAATGGTGTCACCCTGATACCACGGTTTTTTAAAGCGTTTCTGTTTCCATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCA‑GATTCTTCCAGCG‑ATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGAT‑CACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACC  >  NC_007779/667215‑667689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`