BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,782	T→A	7.2%	Y36F (TAT→TTT)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,782	0	T	A	7.2%	359.3 / 2.0	137	Y36F (TAT→TTT)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base T (74/50); new base A (3/8); total (78/59)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TAGCCAGTTTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954544‑955017

   ccAGTTTGTCGCCAACTGACCCCGGGTGATGCGCCCACTCGCCGTGGCAACAACAGTCAACACGGTGGAAGTAAAGGGATCGGCTCCGCAGACAAGACAAAGAATCAGCCCCGGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CATG‑TGCCA‑GTGGGTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGa                                                                                                                                                                                                                                              <  2:970735/239‑1 (MQ=255)

    cAGTTTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGa                                                                                                                                                                                                                                              <  2:1813052/238‑1 (MQ=255)

     aGTTTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATTGaaa                                                                                                                                                                                                                                            >  1:520762/1‑239 (MQ=255)

       tttttCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATaagaaa                                                                                                                                                                                                                                           >  1:1061978/1‑233 (MQ=255)

           tCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑a                                                                                                                                                                                                                                    <  2:2004590/239‑1 (MQ=255)

                 aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCC‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCCGATAGAAAGTC‑TT‑AA‑ACGGa                                                                                                                                                                                                                             >  1:1933275/1‑239 (MQ=255)

                 aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑TA‑ggggg                                                                                                                                                                                                                             >  1:1520036/1‑238 (MQ=255)

                 aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGa                                                                                                                                                                                                                             >  1:534128/1‑239 (MQ=255)

                        ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:1268832/239‑1 (MQ=255)

                        ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:95162/239‑1 (MQ=255)

                        ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:1561252/239‑1 (MQ=255)

                        ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCAG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:1467958/239‑1 (MQ=255)

                        ccAGGTGATGAGCCCACTCGCCTTAGCAACAACAGTCAACACTGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTGTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:878198/239‑1 (MQ=255)

                        ccAGGAGATGCGCCCAGTCGGCTTAGCAACAACAAACAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCTAACCAGTTTTGCCAGGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGGCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:827076/239‑1 (MQ=255)

                         cAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGt                                                                                                                                                                                                                    <  1:1374754/239‑1 (MQ=255)

                            gTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGtt                                                                                                                                                                                                                   >  1:2182834/1‑237 (MQ=255)

                              gATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCt                                                                                                                                                                                                                <  2:2026022/238‑1 (MQ=255)

                               aTGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGCAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTAT‑CGTTGAttt                                                                                                                                                                                                              >  1:1807138/1‑239 (MQ=255)

                                 gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                            >  1:1929962/1‑239 (MQ=255)

                                 gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTtat                                                                                                                                                                                                            >  1:740222/1‑239 (MQ=255)

                                    cccACTCGCCTTAGCAACAACAATCAACACGGCGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTT‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAg                                                                                                                                                                                                          >  1:1654719/1‑238 (MQ=255)

                                               tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CTCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAAACAACCGCctct                                                                                                                                                                                              >  1:62808/1‑239 (MQ=255)

                                               tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑CT‑AA‑GCGGGTGGTT‑CGTTGCTTTATAGACACCCGCctct                                                                                                                                                                                              >  1:415760/1‑239 (MQ=255)

                                               tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑TAT‑CGCCAAATAGAAAAGC‑TT‑AA‑GAGGATGTTT‑TGGTGCTTTTTAGCAACCCCCccct                                                                                                                                                                                              >  2:703498/1‑239 (MQ=255)

                                                 gCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCtt                                                                                                                                                                                             >  1:1651132/1‑238 (MQ=255)

                                                   aacaacaaTCAACACGGTGGAAGTAAAGAGATCGGTTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCg                                                                                                                                                                                           >  2:501548/1‑238 (MQ=255)

                                                     caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCgg                                                                                                                                                                                                                              >  1:186472/1‑202 (MQ=255)

                                                     caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCgg                                                                                                                                                                                                                              <  2:186472/202‑1 (MQ=255)

                                                     caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑C‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCCGATGTTT‑CGTTGCTCTATAGACACCCCCccctcccgc                                                                                                                                                                                         >  1:226257/1‑233 (MQ=255)

                                                          aTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGa                                                                                                                                                                                                                                              >  2:2137945/1‑184 (MQ=255)

                                                          aTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGa                                                                                                                                                                                                                                              <  1:2137945/184‑1 (MQ=255)

                                                           tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     >  1:253164/1‑204 (MQ=255)

                                                           tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:253164/204‑1 (MQ=255)

                                                           tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACtttt                                                                                                                                                                                  >  1:1778340/1‑238 (MQ=255)

                                                               cacGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:339800/200‑1 (MQ=255)

                                                               cacGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     >  1:339800/1‑200 (MQ=255)

                                                                acGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCCGAAAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACAACCGCCTCTTCGGCCACTTTGGCCAt                                                                                                                                                                             >  1:1550347/1‑239 (MQ=255)

                                                                  ggTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTTT‑CGTTGGTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCAtt                                                                                                                                                                            >  1:921189/1‑238 (MQ=255)

                                                                    tGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGAAGTTT‑TGTTGCTTTATAGACAACCCCCCCTTCGGGCAACTTGGCCCtttg                                                                                                                                                                          >  2:230955/1‑236 (MQ=255)

                                                                       aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCCGATAGAAAATC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      >  1:2139549/1‑239 (MQ=255)

                                                                       aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GAGGAAGGTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCCCTTTGGCCATTgcaaca                                                                                                                                                                      >  1:225662/1‑239 (MQ=255)

                                                                                gcgcGGCCCCGCACAAAAAACAAAAAACCGGCCCCAGAGAAAAG‑AAAGGCCCCCAACCCGGTTTTCCAAGGCCGAGGGCATTTGTG‑CCTG‑GGCCA‑GGGGTTGTTGGAAAATAAAAAACG‑AATGTGATT‑GAAAAGAAAACCCC‑GG‑C‑G‑T‑AAC‑CCCCAGAAAAAAAATCTTT‑GA‑GGGGATTTTT‑CGTTGCTATAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                              <  2:1551781/235‑1 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCAGATAGAACGTC‑TT‑AA‑GAGGATGTTT‑CGTTGTTTTTTAGACACCCGCCCCCTCGGCCAACTTGGCCATTGCAGCAGGGAGTaaaa                                                                                                                                                            >  1:342084/1‑239 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:1771233/1‑239 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:1354176/1‑239 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑AA‑GCGGAGGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:1086069/1‑239 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑AGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCGGCCACTTTGGCCATTGCATCAGGAAGTaaaa                                                                                                                                                            >  2:1605443/1‑239 (MQ=255)

                                                                                 aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:475238/1‑239 (MQ=255)

                                                                                   cGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCAGGAAGAAAATC‑CT‑TA‑GCGGGTTTTT‑CCTTGCTTTATAGGCACCCGTCCCTTCGCTCACTTTGTCCATTTCCACATGAAGGaaaaaa                                                                                                                                                          >  2:618718/1‑237 (MQ=255)

                                                                                    ggCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAGAGAAAATC‑TT‑AA‑GCGGATGTTT‑TGTTGCTTTATATACACCCCCCTCTCCCGGCACTTTGGCCCTTGCCGCCGGAAGTaaaaaa                                                                                                                                                          >  2:816349/1‑236 (MQ=255)

                                                                                           cAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAACTCAAAAgg                                                                                                                                                  >  1:1002559/1‑239 (MQ=255)

                                                                                                aacaCAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGGTGTTT‑CGCTGCTTTATAAAAACCCGCCCCTTCGGCCACCTTGGCCATTGCCGCAGGAAGGAAAAGATCAAAAGGGTTg                                                                                                                                              >  2:1633250/1‑238 (MQ=255)

                                                                                                     aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                            >  1:283159/1‑171 (MQ=255)

                                                                                                     aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                            <  2:283159/171‑1 (MQ=255)

                                                                                                          aTCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑A‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:569787/157‑1 (MQ=255)

                                                                                                            cAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑ACGGATGTTT‑CCTTGCCTTATAGACACCCCCCCCTTCCGACAACTTGGCCAATGCCGCAGGAAGGTAAAGACCAAAAGGGTTGGCAGCTTTcaca                                                                                                                                  >  2:375210/1‑238 (MQ=255)

                                                                                                                   agagagAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTA‑TT‑AA‑GCGGATGTTT‑AGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTACAGCAGGAAGAAAAAGATCAAAAGGGTTGTAAGCTTTCACAATAActc                                                                                                                           >  1:1270059/1‑238 (MQ=255)

                                                                                                                     agagaAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                         >  1:1315914/1‑238 (MQ=255)

                                                                                                                      gagaAGCAAATGCCCCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTAATATAGAAGACG‑AATGCGATT‑GAGATAAAACCCCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                         <  1:961928/237‑1 (MQ=255)

                                                                                                                       agaAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTTACACTAActctctct                                                                                                                      >  1:1487393/1‑239 (MQ=255)

                                                                                                                        gaAGCAAAGGCCCCCAACCAGTTTTGCCAGGCCGAAGGGAA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGTTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACCCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGGTTGTTT‑CGTTGCTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTgcag                                                                                                                                                                        <  1:1225478/188‑1 (MQ=255)

                                                                                                                         aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAACATCAAAAGGGTTGTCAGCTTTCACACTAACTCCATCttt                                                                                                                    >  1:1126407/1‑239 (MQ=255)

                                                                                                                         aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑TGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACAATAACCTTCTCttt                                                                                                                    >  1:123795/1‑239 (MQ=255)

                                                                                                                         aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACCTTGGGCATTGCAGCAGGAAGTAAAAGAACAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCttt                                                                                                                    >  1:1077850/1‑239 (MQ=255)

                                                                                                                         aaGAAAATGCCGCAAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑GGCCA‑GTGGTTGCTGTGATATAGAAGACA‑AATGCGATT‑GAGATAAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAAAGAAAGTT‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      <  1:453257/189‑1 (MQ=255)

                                                                                                                            cAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAGACACCCGCCCCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGTAAAAAAACAAAAGGGTTGTCAGCTTTCAACCTTACCCTCTCTttatt                                                                                                                 >  1:581271/1‑239 (MQ=255)

                                                                                                                             aaaGGCCACCAACCAGTTTTGCCATGCGGAAGGTCA‑TTGTG‑CCTG‑TGCCG‑GTGGTTGGTTTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAGGTC‑TT‑AA‑GCGGTTGTTT‑CTTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTttatta                                                                                                                <  1:816349/239‑1 (MQ=255)

                                                                                                                               cttcccccAACAGGTTTGGCCGTGCCGAGGGTTA‑GTGAG‑CCTG‑TGCTG‑GGTGTGGCGTTTATATAGAAGATG‑AATGAGGAT‑GAGA‑GAAAACCCC‑GGTCGG‑A‑AAT‑CACCATGTAGAATCTT‑GA‑AA‑GGTTTTTTTT‑TGTTTTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTACCTCTCTCTTTATTAAg                                                                                                              <  2:2059780/233‑1 (MQ=255)

                                                                                                                                 gccgccAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTc                                                                                                            >  1:542654/1‑239 (MQ=255)

                                                                                                                                 gccgccAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGACATTGCAGCCGGAAGGAAAAGATCAAAAGGGATGTCAGCTTTCACCCTAACTCCCTCTCTATTAAGTc                                                                                                            >  1:1882603/1‑239 (MQ=255)

                                                                                                                                       accattttttGCGATGCCG‑AGGGGA‑TTGTG‑CCTG‑GGCCAGGTGGTTGGTGTAATATAGAAGAGA‑AGTGCGATT‑GAGATAAAACCACC‑GG‑GGT‑T‑ACT‑CGCAAGATAAAAAGTT‑AT‑AA‑GCGTATTTTT‑TGTTTTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  2:2182834/233‑1 (MQ=255)

                                                                                                                                                  ccATGCCGAAGGGTA‑‑TGTG‑GCTGCTGCGT‑GGGGTTGGTGTGATATAGAAGACA‑AATGCGATT‑GAGAAGAAACCACC‑GG‑CGG‑T‑ACT‑CGCAAAATAGAAGGTC‑TA‑AG‑GCGGATGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            <  2:1061978/238‑1 (MQ=255)

                                                                                                                                                   aaTGCCGAAGGGCA‑TGGTG‑CCTG‑GGCCA‑GTGTTTGCTGTAATATAAAAGACA‑AATGGAATT‑GAGAAAAAAACCCC‑GG‑CGT‑T‑AAT‑CGCCAGAAAGAAAGTC‑TA‑AA‑GGGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1605443/238‑1 (MQ=255)

                                                                                                                                                        tgaagggggtttGGG‑CCGG‑TGCAG‑GGGGTTGTTGTAATAAAAAAGAGG‑AATG‑GATTAGAAAT‑AAAACCCC‑GG‑GGGAT‑AAC‑CGCAAAAAAAAAAGTT‑TA‑AA‑GGGGTTTTTT‑TGTTTTTTAAAGGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCACTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:534128/228‑1 (MQ=255)

                                                                                                                                                        cGAAGGGGA‑TTTGGCCCTG‑TGCGG‑GGGGTTGTGGAGATAAAAAACACG‑AATGCGTTA‑GAGATAAAAAC‑CC‑GG‑GGG‑TAAAC‑CGCAAGATAGAAATTT‑TA‑AA‑GCGTTTTTTT‑CTTTTTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:1112368/238‑1 (MQ=255)

                                                                                                                                                                      g‑cctg‑tgcaA‑GTGGTTGCTGTGAAAGAGAAGACG‑AATGCGTTG‑GAGATAAAAGCCCC‑GG‑CGG‑T‑AAT‑CGCAAGATAGAAAGTT‑TT‑AG‑GGGGATGTTT‑GGTTTCTTTAAAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATgaga                                                                         <  1:501548/238‑1 (MQ=255)

                                                                                                                                                                         ctg‑tgcaA‑GTGTTTGCTGGGATAAAGAAGACG‑AATGCTATT‑GAGATGAAACCCCC‑GG‑CGG‑T‑AAT‑CCCCAGAAAGAATGTC‑TT‑AG‑GCGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  1:1576396/238‑1 (MQ=255)

                                                                                                                                                                          tg‑tggcA‑GTGGTGTCTGTGAAATAAAAGGAC‑AATGCGATT‑GAATGGAAACCACC‑GG‑AGT‑A‑ATT‑CGCTAGAAAGACAGTG‑AT‑AA‑GCGATTGTTT‑TGTCGCTATACAGCCCTCCGCCTCTTCGGCCACTTTGGCAATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAt                                                                      <  2:441526/238‑1 (MQ=255)

                                                                                                                                                                             tgccA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCCGGGAGTAAAAGATCCAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTTATTCGGCGACGTGATACTAACAACACATTATAGCTGAGCAATTGa                                                                   >  1:1156136/1‑239 (MQ=255)

                                                                                                                                                                               ccA‑GTGTTTGGTGTGATATA‑AAAACGAAAGGAGTTT‑GAAATAAAAACCCC‑GG‑CGG‑T‑AAC‑CGCCAGATA‑AAAGTT‑TT‑AAGGGGGTTGTTT‑GTTTGCTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGat                                                                  <  1:306231/238‑1 (MQ=255)

                                                                                                                                                                                 gtgTGGTTGCTGTAAAATAGGAGAGT‑AATGTGATT‑GAGA‑ACAACCACC‑GG‑ACG‑T‑AATCCCCCAGAT‑TAAAGTT‑TTGAA‑GCTGTTTTTT‑TGTTTCTTTAAAGACCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:415760/236‑1 (MQ=255)

                                                                                                                                                                                 cattggttggtGGAATAAAAAAGACA‑AGTGAGA‑T‑GAGAAGAAAACCCCGGG‑GGG‑T‑AAT‑CCCAAAATAAAAAGTT‑TA‑AG‑GCGGTTTTTT‑GTTATTTTTAAACAACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:2139549/235‑1 (MQ=255)

                                                                                                                                                                                 a‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                               >  1:1917039/1‑239 (MQ=255)

                                                                                                                                                                                    tGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGa                                                             >  1:263906/1‑239 (MQ=255)

                                                                                                                                                                                      ttggttgaaataaAGAAAACG‑AATGATATT‑GAGATAAAACCCCC‑GG‑GGT‑A‑AAC‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGTTTGTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                            <  1:306429/229‑1 (MQ=255)

                                                                                                                                                                                        tGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCCGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCat                                                         >  1:1085517/1‑239 (MQ=255)

                                                                                                                                                                                               atatAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGATTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATTAAAAAATGATATAGATCATAtctc                                                    >  1:1095547/1‑237 (MQ=255)

                                                                                                                                                                                                     aagaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAAATAGAAAGTC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACCCTAACTCTCTCTTTATTAAGTCGGCGACGAGAAACTAACAAAGCATTATAGATGAGAAATTGCTATAGATCATTTTCCGCCTGGCt                                            >  2:746082/1‑239 (MQ=255)

                                                                                                                                                                                                       gaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAATTTGatat                                                                >  1:1127563/1‑217 (MQ=255)

                                                                                                                                                                                                          g‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctctct                                                                                                                      >  1:832973/1‑160 (MQ=255)

                                                                                                                                                                                                            aaTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCCCCTCTTCGGCCACTTTTGCCATTTCAGCAGGAAGTAAAAAATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACCAGACACTAACAAAACATTAAAGATGAGAAAATGATAAAGATCATAACTCGCCTTGCCTTTAgg                                      >  2:1690827/1‑239 (MQ=255)

                                                                                                                                                                                                             aTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑TA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCCTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGAAGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                  >  1:834429/1‑226 (MQ=255)

                                                                                                                                                                                                                  aTT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCgg                                                                                                                                                                                          >  1:370741/1‑85 (MQ=255)

                                                                                                                                                                                                                              aaCACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGAAGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGAAAAAGAt                                                                                                                                                         >  1:1518326/1‑107 (MQ=255)

                                                                                                                                                                                                                                              caT‑CGCAGGATAGAATTTC‑AG‑AA‑GCGTTTGTTT‑AGTTTCTTTACGGCCCCCCGCCTCTTCGGCCACTTTTGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGATAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAAt         <  2:1151984/238‑1 (MQ=255)

                                                                                                                                                                                                                                               aT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGTTTTTTT‑CGTTGTTTAAAAGACAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:736017/239‑1 (MQ=255)

                                                                                                                                                                                                                                               aT‑CGCACGATAAAAATTC‑TC‑AT‑GCGGTTTTTT‑TGTATCTTTAGATACACCCGCCTCTTCGGCCTCTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:947249/239‑1 (MQ=255)

                                                                                                                                                                                                                                                agcGCCAGATA‑AAAATG‑TT‑AA‑GCGGGTGTTTCCTTTGCTTTAGAGTCAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:1421079/237‑1 (MQ=255)

                                                                                                                                                                                                                                                     cAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAg   >  1:979501/1‑239 (MQ=255)

                                                                                                                                                                                                                                                      agaaagaaCGTC‑TA‑AA‑GCGTATGTTT‑GGTTTCTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:618718/239‑1 (MQ=255)

TAGCCAGTTTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TTGTG‑CCTG‑TGCCA‑GTGGTTGCTGTGATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954544‑955017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`