BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,788	G→A	8.9%	T34I (ACT→ATT)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,788	0	G	A	8.9%	278.8 / 2.1	129	T34I (ACT→ATT)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base G (63/42); new base A (10/2); total (73/56)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954552‑955017

ttttCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTc                                                                                                                                                                                                                                         <  1:1268364/239‑1 (MQ=255)

   tCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑a                                                                                                                                                                                                                                    <  2:2004590/239‑1 (MQ=255)

         aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCC‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCCGATAGAAAGTC‑TT‑AA‑ACGGa                                                                                                                                                                                                                             >  1:1933275/1‑239 (MQ=255)

         aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑TA‑ggggg                                                                                                                                                                                                                             >  1:1520036/1‑238 (MQ=255)

         aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGa                                                                                                                                                                                                                             >  1:534128/1‑239 (MQ=255)

         aCTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGGTAAAAAGTA‑TT‑AA‑GCGGa                                                                                                                                                                                                                             >  1:2128652/1‑239 (MQ=255)

              ccccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGttt                                                                                                                                                                                                                        <  2:375253/239‑1 (MQ=255)

                ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:1561252/239‑1 (MQ=255)

                ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:95162/239‑1 (MQ=255)

                ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:1268832/239‑1 (MQ=255)

                ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCAG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  1:1467958/239‑1 (MQ=255)

                ccAGGTGATGAGCCCACTCGCCTTAGCAACAACAGTCAACACTGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTGTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:878198/239‑1 (MQ=255)

                ccAGGAGATGCGCCCAGTCGGCTTAGCAACAACAAACAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCTAACCAGTTTTGCCAGGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGGCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:827076/239‑1 (MQ=255)

                 cAGGTGATGCGCCGACTCGCCATCGCAACAACAATCAACATGGTGGAAGTAAAGAGATCGGCTCCGCAGACAGCACAAAGAATCAGCGCAAGAGAGAAGCAAAGGCCGCCAACCAGTTTTGGCATGCTGGAGGACA‑TA‑TTG‑CCTG‑TGCCA‑GTGGTTGATGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATTAAAACACC‑GG‑CCG‑T‑AAT‑CGGCAGATTGAAAGTC‑TT‑AA‑GCGTAAGTTA‑CGt                                                                                                                                                                                                                    <  2:1787879/239‑1 (MQ=255)

                 cAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGt                                                                                                                                                                                                                    <  1:531359/239‑1 (MQ=255)

                    gTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGtt                                                                                                                                                                                                                   >  1:2182834/1‑237 (MQ=255)

                      gATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCt                                                                                                                                                                                                                <  2:2026022/238‑1 (MQ=255)

                        tGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTta                                                                                                                                                                                                             >  1:1545200/1‑239 (MQ=255)

                         gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTtat                                                                                                                                                                                                            >  1:740222/1‑239 (MQ=255)

                                       tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CTCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAAACAACCGCctct                                                                                                                                                                                              >  1:62808/1‑239 (MQ=255)

                                       tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑CT‑AA‑GCGGGTGGTT‑CGTTGCTTTATAGACACCCGCctct                                                                                                                                                                                              >  1:415760/1‑239 (MQ=255)

                                       tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑TAT‑CGCCAAATAGAAAAGC‑TT‑AA‑GAGGATGTTT‑TGGTGCTTTTTAGCAACCCCCccct                                                                                                                                                                                              >  2:703498/1‑239 (MQ=255)

                                         gCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCtt                                                                                                                                                                                             >  1:1651132/1‑238 (MQ=255)

                                           aacaacaaTCAACACGGTGGAAGTAAAGAGATCGGTTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCg                                                                                                                                                                                           >  2:501548/1‑238 (MQ=255)

                                             caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCgg                                                                                                                                                                                                                              >  1:186472/1‑202 (MQ=255)

                                             caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑C‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCCGATGTTT‑CGTTGCTCTATAGACACCCCCccctcccgc                                                                                                                                                                                         >  1:226257/1‑233 (MQ=255)

                                                   tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:253164/204‑1 (MQ=255)

                                                   tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     >  1:253164/1‑204 (MQ=255)

                                                   tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACtttt                                                                                                                                                                                  >  1:1778340/1‑238 (MQ=255)

                                                       cacGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:339800/200‑1 (MQ=255)

                                                       cacGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     >  1:339800/1‑200 (MQ=255)

                                                               aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCCGATAGAAAATC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      >  1:2139549/1‑239 (MQ=255)

                                                               aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GAGGAAGGTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCCCTTTGGCCATTgcaaca                                                                                                                                                                      >  1:225662/1‑239 (MQ=255)

                                                                        gcgcGGCCCCGCACAAAAAACAAAAAACCGGCCCCAGAGAAAAG‑AAAGGCCCCCAACCCGGTTTTCCAAGGCCGAGGGCATTT‑GTG‑CCTG‑GGCCA‑GGGGTTGTTGGA‑AAATAAAAAACG‑AATGTGATT‑GAAAAGAAAACCCC‑GG‑C‑G‑T‑AAC‑CCCCAGAAAAAAAATCTTT‑GA‑GGGGATTTTT‑CGTTGCTATAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                              <  2:1551781/235‑1 (MQ=255)

                                                                         aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCAGATAGAACGTC‑TT‑AA‑GAGGATGTTT‑CGTTGTTTTTTAGACACCCGCCCCCTCGGCCAACTTGGCCATTGCAGCAGGGAGTaaaa                                                                                                                                                            >  1:342084/1‑239 (MQ=255)

                                                                         aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:1771233/1‑239 (MQ=255)

                                                                         aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  1:1354176/1‑239 (MQ=255)

                                                                         aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTTATAAAAACCCACCACTTCTGCCACTTTTGCCCTTGCAGCCGGAAGGaaac                                                                                                                                                            >  2:2195213/1‑238 (MQ=255)

                                                                           cGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAAGC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGa                                                                                                                                                          >  1:953187/1‑239 (MQ=255)

                                                                                   cAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAACTCAAAAgg                                                                                                                                                  >  1:1002559/1‑239 (MQ=255)

                                                                                        aacaCAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGGTGTTT‑CGCTGCTTTATAAAAACCCGCCCCTTCGGCCACCTTGGCCATTGCCGCAGGAAGGAAAAGATCAAAAGGGTTg                                                                                                                                              >  2:1633250/1‑238 (MQ=255)

                                                                                             aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                            >  1:283159/1‑171 (MQ=255)

                                                                                             aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                            <  2:283159/171‑1 (MQ=255)

                                                                                                  aTCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     >  1:569787/1‑157 (MQ=255)

                                                                                                  aTCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcac                                                                                                                                   >  1:401259/1‑239 (MQ=255)

                                                                                                  aTCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑A‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑Cg                                                                                                                                                                                                                     <  2:569787/157‑1 (MQ=255)

                                                                                                           agagagAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAActc                                                                                                                           >  1:1469938/1‑238 (MQ=255)

                                                                                                             ggagaAACAACTCCCGCCACCCATTTTGGCCATGCGGAGGGGCT‑TT‑GTG‑CCGG‑TGCCG‑GTGGTTGTTGTG‑TTAAAGAAGACG‑AAGGCATTG‑GAGATAAAACCACG‑GG‑CGG‑T‑ACC‑CGCAAGATAGAAAGTT‑TA‑AA‑GGGGGTTTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                         <  2:614333/237‑1 (MQ=255)

                                                                                                             agagaAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                         >  1:1315914/1‑238 (MQ=255)

                                                                                                                gaAGCAAAGGCCCCCAACCAGTTTTGCCAGGCCGAAGGGAA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGTTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACCCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGGTTGTTT‑CGTTGCTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTgcag                                                                                                                                                                        <  1:1225478/188‑1 (MQ=255)

                                                                                                                 aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTTCTTTTTAGAAACCCCCCCCTTCGGCCCCTTTGGCCATTTCAGCAGGAAGTAAAAAAACAAAAAGGTTTGCCACTTTCACACCAACCCCCTCttt                                                                                                                    >  2:1576396/1‑239 (MQ=255)

                                                                                                                 aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAACATCAAAAGGGTTGTCAGCTTTCACACTAACTCCATCttt                                                                                                                    >  1:1126407/1‑239 (MQ=255)

                                                                                                                 aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑TGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACAATAACCTTCTCttt                                                                                                                    >  1:123795/1‑239 (MQ=255)

                                                                                                                 aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACCTTGGGCATTGCAGCAGGAAGTAAAAGAACAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCttt                                                                                                                    >  1:1077850/1‑239 (MQ=255)

                                                                                                                 aaGAAAATGCCGCAAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑GGCCA‑GTGGTTGCTGTG‑ATATAGAAGACA‑AATGCGATT‑GAGATAAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAAAGAAAGTT‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      <  1:453257/189‑1 (MQ=255)

                                                                                                                    cAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAGACACCCGCCCCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGTAAAAAAACAAAAGGGTTGTCAGCTTTCAACCTTACCCTCTCTttatt                                                                                                                 >  1:581271/1‑239 (MQ=255)

                                                                                                                     aaaGGCCACCAACCAGTTTTGCCATGCGGAAGGTCA‑TT‑GTG‑CCTG‑TGCCG‑GTGGTTGGTTTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAGGTC‑TT‑AA‑GCGGTTGTTT‑CTTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTttatta                                                                                                                <  1:816349/239‑1 (MQ=255)

                                                                                                                       cttcccccAACAGGTTTGGCCGTGCCGAGGGTTA‑GT‑GAG‑CCTG‑TGCTG‑GGTGTGGCGTTT‑ATATAGAAGATG‑AATGAGGAT‑GAGA‑GAAAACCCC‑GGTCGG‑A‑AAT‑CACCATGTAGAATCTT‑GA‑AA‑GGTTTTTTTT‑TGTTTTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTACCTCTCTCTTTATTAAg                                                                                                              <  2:2059780/233‑1 (MQ=255)

                                                                                                                       aTGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGGAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAg                                                                                                              >  1:464319/1‑239 (MQ=255)

                                                                                                                         gccgccAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGACATTGCAGCCGGAAGGAAAAGATCAAAAGGGATGTCAGCTTTCACCCTAACTCCCTCTCTATTAAGTc                                                                                                            >  1:1882603/1‑239 (MQ=255)

                                                                                                                               accattttttGCGATGCCG‑AGGGGA‑TT‑GTG‑CCTG‑GGCCAGGTGGTTGGTGTA‑ATATAGAAGAGA‑AGTGCGATT‑GAGATAAAACCACC‑GG‑GGT‑T‑ACT‑CGCAAGATAAAAAGTT‑AT‑AA‑GCGTATTTTT‑TGTTTTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  2:2182834/233‑1 (MQ=255)

                                                                                                                                          ccATGCCGAAGGGTA‑‑T‑GTG‑GCTGCTGCGT‑GGGGTTGGTGTG‑ATATAGAAGACA‑AATGCGATT‑GAGAAGAAACCACC‑GG‑CGG‑T‑ACT‑CGCAAAATAGAAGGTC‑TA‑AG‑GCGGATGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            <  2:1061978/238‑1 (MQ=255)

                                                                                                                                           aaTGCCGAAGGGCA‑TG‑GTG‑CCTG‑GGCCA‑GTGTTTGCTGTA‑ATATAAAAGACA‑AATGGAATT‑GAGAAAAAAACCCC‑GG‑CGT‑T‑AAT‑CGCCAGAAAGAAAGTC‑TA‑AA‑GGGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1605443/238‑1 (MQ=255)

                                                                                                                                            caggcggaGGGCA‑TTGGTG‑CTGG‑TG‑CA‑GGGGTTGCTGGGAATAAAGAAGAGA‑ATTGGGTTG‑GTGATAAAACCGCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTT‑TT‑AG‑GGGGTTTTTT‑TTTTGTTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  2:740222/232‑1 (MQ=255)

                                                                                                                                                tgaagggggttt‑GGG‑CCGG‑TGCAG‑GGGGTTGTTGTA‑ATAAAAAAGAGG‑AATG‑GATTAGAAAT‑AAAACCCC‑GG‑GGGAT‑AAC‑CGCAAAAAAAAAAGTT‑TA‑AA‑GGGGTTTTTT‑TGTTTTTTAAAGGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCACTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:534128/228‑1 (MQ=255)

                                                                                                                                                cGAAGGGGA‑TT‑TGGCCCTG‑TGCGG‑GGGGTTGTGGAG‑ATAAAAAACACG‑AATGCGTTA‑GAGATAAAAAC‑CC‑GG‑GGG‑TAAAC‑CGCAAGATAGAAATTT‑TA‑AA‑GCGTTTTTTT‑CTTTTTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:1112368/238‑1 (MQ=255)

                                                                                                                                                        a‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGGTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATAAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCCTTATTAAGTCGGCGGCGAGAAACTAACAAAACATTATAGAt                                                                             >  2:852943/1‑239 (MQ=255)

                                                                                                                                                               g‑cctg‑tgcaA‑GTGGTTGCTGTG‑AAAGAGAAGACG‑AATGCGTTG‑GAGATAAAAGCCCC‑GG‑CGG‑T‑AAT‑CGCAAGATAGAAAGTT‑TT‑AG‑GGGGATGTTT‑GGTTTCTTTAAAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATgaga                                                                         <  1:501548/238‑1 (MQ=255)

                                                                                                                                                                  ctg‑tgcaA‑GTGTTTGCTGGG‑ATAAAGAAGACG‑AATGCTATT‑GAGATGAAACCCCC‑GG‑CGG‑T‑AAT‑CCCCAGAAAGAATGTC‑TT‑AG‑GCGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  1:1576396/238‑1 (MQ=255)

                                                                                                                                                                   tg‑tggcA‑GTGGTGTCTGTG‑AAATAAAAGGAC‑AATGCGATT‑GAATGGAAACCACC‑GG‑AGT‑A‑ATT‑CGCTAGAAAGACAGTG‑AT‑AA‑GCGATTGTTT‑TGTCGCTATACAGCCCTCCGCCTCTTCGGCCACTTTGGCAATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAt                                                                      <  2:441526/238‑1 (MQ=255)

                                                                                                                                                                    tggtgcggggggTTTCTGAG‑AAAAAGAAGGCG‑AATGCGATT‑GGGAGAAATACCCC‑GG‑TGA‑T‑AAC‑CGCAAAATACAGAGTC‑TG‑AA‑GGGTGTGTTT‑CTTGTTTTTAGAGCCTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  2:1917039/228‑1 (MQ=255)

                                                                                                                                                                    g‑tgccA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAAAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTTCAGCAGGAAGGAAAAAATAAACAAGGTTGTCAGCTTTCACACTAACTCTCTCCTTATTAAGTCGGCGACGCGATACTAACAAAACATTATACATGAGAAATTg                                                                    >  1:1905052/1‑239 (MQ=255)

                                                                                                                                                                        ccA‑GTGTTTGGTGTG‑ATATA‑AAAACGAAAGGAGTTT‑GAAATAAAAACCCC‑GG‑CGG‑T‑AAC‑CGCCAGATA‑AAAGTT‑TT‑AAGGGGGTTGTTT‑GTTTGCTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGat                                                                  <  1:306231/238‑1 (MQ=255)

                                                                                                                                                                          gtgTGGTTGCTGTA‑AAATAGGAGAGT‑AATGTGATT‑GAGA‑ACAACCACC‑GG‑ACG‑T‑AATCCCCCAGAT‑TAAAGTT‑TTGAA‑GCTGTTTTTT‑TGTTTCTTTAAAGACCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:415760/236‑1 (MQ=255)

                                                                                                                                                                          cattggttggtGGA‑ATAAAAAAGACA‑AGTGAGA‑T‑GAGAAGAAAACCCCGGG‑GGG‑T‑AAT‑CCCAAAATAAAAAGTT‑TA‑AG‑GCGGTTTTTT‑GTTATTTTTAAACAACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:2139549/235‑1 (MQ=255)

                                                                                                                                                                          a‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                               >  1:1917039/1‑239 (MQ=255)

                                                                                                                                                                             tGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGa                                                             >  1:263906/1‑239 (MQ=255)

                                                                                                                                                                              ggTTGCGGTG‑ATAAAGAAGACA‑AAGGGGTTT‑GAGATAAAAACACC‑GG‑CGG‑T‑AAT‑CGCAAAATAGAAAGTC‑TT‑AA‑GCGGTTTTTT‑CTTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                            <  1:1633250/239‑1 (MQ=255)

                                                                                                                                                                                 tGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCCGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCat                                                         >  1:1085517/1‑239 (MQ=255)

                                                                                                                                                                                 tGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGTAAAAGATCAAAAAGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       >  1:32523/1‑209 (MQ=255)

                                                                                                                                                                                         atatAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGATTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATTAAAAAATGATATAGATCATAtctc                                                    >  1:1095547/1‑237 (MQ=255)

                                                                                                                                                                                          taaagaagcCA‑AATGCAATT‑GATA‑GAAAACCCC‑GGTCTT‑T‑AAC‑CCCAAAATAAAAAGTC‑TA‑AA‑GCGGTTTTTT‑TTTTTTTTAAAACAACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:852943/236‑1 (MQ=255)

                                                                                                                                                                                               aagaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAAATAGAAAGTC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACCCTAACTCTCTCTTTATTAAGTCGGCGACGAGAAACTAACAAAGCATTATAGATGAGAAATTGCTATAGATCATTTTCCGCCTGGCt                                            >  2:746082/1‑239 (MQ=255)

                                                                                                                                                                                                 gaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGAGTTT‑TGTTGCCTTATAAACACCCGCCCCTTCGGCCAATTTTGCCATTGCAGCAGGAAGTAAAAAATCAAAAAGGGTGTCAGCTTTCACACTAACTCTCTCTTTTTTAAGTCCGCGACGAGATACTAACAAAACATTATAAATGAGAAATTTATATAGAACATTTCTCTCCTGGGTta                                          >  2:1388847/1‑239 (MQ=255)

                                                                                                                                                                                                 gaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAATTTGatat                                                                >  1:1127563/1‑217 (MQ=255)

                                                                                                                                                                                                    g‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctctct                                                                                                                      >  1:832973/1‑160 (MQ=255)

                                                                                                                                                                                                    aaaacgctttt‑GAGAAGAAACCCCC‑GG‑CGG‑T‑AAG‑CCCAATTTAGAACTTC‑TA‑AA‑GCGGTTGTCT‑CTTTTCTTTAAAGACTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTtata                                        <  1:108216/230‑1 (MQ=255)

                                                                                                                                                                                                      aaTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCCCCTCTTCGGCCACTTTTGCCATTTCAGCAGGAAGTAAAAAATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACCAGACACTAACAAAACATTAAAGATGAGAAAATGATAAAGATCATAACTCGCCTTGCCTTTAgg                                      >  2:1690827/1‑239 (MQ=255)

                                                                                                                                                                                                       aTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAATCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAACATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAg                                       >  1:1300261/1‑237 (MQ=255)

                                                                                                                                                                                                       aTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑TA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCCTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGAAGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                  >  1:834429/1‑226 (MQ=255)

                                                                                                                                                                                                                 aaaaaaaaccccc‑GG‑CGA‑T‑AAT‑CGCAAGATAGAAATTC‑TG‑AG‑GTGTTTTTTT‑CTTGGCTTAAAAACCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACt                             <  2:263906/227‑1 (MQ=255)

                                                                                                                                                                                                                        aaCACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGAAGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGAAAAAGAt                                                                                                                                                         >  1:1518326/1‑107 (MQ=255)

                                                                                                                                                                                                                                         aT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGTTTTTTT‑CGTTGTTTAAAAGACAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:736017/239‑1 (MQ=255)

                                                                                                                                                                                                                                         aT‑CGCACGATAAAAATTC‑TC‑AT‑GCGGTTTTTT‑TGTATCTTTAGATACACCCGCCTCTTCGGCCTCTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:947249/239‑1 (MQ=255)

                                                                                                                                                                                                                                          agcGCCAGATA‑AAAATG‑TT‑AA‑GCGGGTGTTTCCTTTGCTTTAGAGTCAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:1421079/237‑1 (MQ=255)

                                                                                                                                                                                                                                            cGCCAGATAGAATGTC‑TT‑AA‑GCGGTTTTTT‑TTTTTTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGACACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACg      <  1:1690827/239‑1 (MQ=255)

                                                                                                                                                                                                                                               cAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAg   >  1:979501/1‑239 (MQ=255)

                                                                                                                                                                                                                                                agaaagaaCGTC‑TA‑AA‑GCGTATGTTT‑GGTTTCTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:618718/239‑1 (MQ=255)

                                                                                                                                                                                                                                                aGATAAAAATTT‑TT‑AC‑GCGGTTTTTT‑CGTTTATCTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:1388847/239‑1 (MQ=255)

                                                                                                                                                                                                                                                   gaGAAATTC‑TT‑GA‑GGGGTTTTTT‑CTTGGTTTTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:714882/235‑1 (MQ=255)

TTTTCGCCAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954552‑955017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`