BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,809	G→T	14.0%	A27E (GCA→GAA)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,809	0	G	T	14.0%	215.7 / 4.1	105	A27E (GCA→GAA)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base G (64/24); new base T (2/15); total (66/39)
Fisher's exact test for biased strand distribution p-value = 3.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

Only 100 of 105 total aligned reads displayed.

GATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGA  >  NC_007779/954574‑955027

 aTGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGCAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTAT‑CGTTGAttt                                                                                                                                                                                                                        >  1:1807138/1‑239 (MQ=255)

   gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                                      >  1:1929962/1‑239 (MQ=255)

   gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTtat                                                                                                                                                                                                                      >  1:740222/1‑239 (MQ=255)

      cccACTCGCCTTAGCAACAACAATCAACACGGCGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTT‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAg                                                                                                                                                                                                                    >  1:1654719/1‑238 (MQ=255)

                 tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CTCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAAACAACCGCctct                                                                                                                                                                                                        >  1:62808/1‑239 (MQ=255)

                 tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑CT‑AA‑GCGGGTGGTT‑CGTTGCTTTATAGACACCCGCctct                                                                                                                                                                                                        >  1:415760/1‑239 (MQ=255)

                 tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑TAT‑CGCCAAATAGAAAAGC‑TT‑AA‑GAGGATGTTT‑TGGTGCTTTTTAGCAACCCCCccct                                                                                                                                                                                                        >  2:703498/1‑239 (MQ=255)

                   gCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCtt                                                                                                                                                                                                       >  1:1651132/1‑238 (MQ=255)

                     aacaacaaTCAACACGGTGGAAGTAAAGAGATCGGTTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCg                                                                                                                                                                                                     >  2:501548/1‑238 (MQ=255)

                       caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑C‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCCGATGTTT‑CGTTGCTCTATAGACACCCCCccctcccgc                                                                                                                                                                                                   >  1:226257/1‑233 (MQ=255)

                             tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACtttt                                                                                                                                                                                            >  1:1778340/1‑238 (MQ=255)

                                  acGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCCGAAAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACAACCGCCTCTTCGGCCACTTTGGCCAt                                                                                                                                                                                       >  1:1550347/1‑239 (MQ=255)

                                    ggTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTTT‑CGTTGGTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCAtt                                                                                                                                                                                      >  1:921189/1‑238 (MQ=255)

                                      tGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGAAGTTT‑TGTTGCTTTATAGACAACCCCCCCTTCGGGCAACTTGGCCCtttg                                                                                                                                                                                    >  2:230955/1‑236 (MQ=255)

                                         aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCCGATAGAAAATC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                                >  1:2139549/1‑239 (MQ=255)

                                         aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GAGGAAGGTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCCCTTTGGCCATTgcaaca                                                                                                                                                                                >  1:225662/1‑239 (MQ=255)

                                                  gcgcGGCCCCGCACAAAAAACAAAAAACCGGCCCCAGAGAAAAG‑AAAGGCCCCCAACCCGGTTTTCCAAGGCCGAGGGCATTT‑GTG‑CCTG‑GGCCA‑GGGGTTGTTGGA‑AAATAAAAAACG‑AATGTGATT‑GAAAAGAAAACCCC‑GG‑C‑G‑T‑AAC‑CCCCAGAAAAAAAATCTTT‑GA‑GGGGATTTTT‑CGTTGCTATAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                                        <  2:1551781/235‑1 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑TGCCAGATAGAACGTC‑TT‑AA‑GAGGATGTTT‑CGTTGTTTTTTAGACACCCGCCCCCTCGGCCAACTTGGCCATTGCAGCAGGGAGTaaaa                                                                                                                                                                      >  1:342084/1‑239 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                                      >  1:1771233/1‑239 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                                      >  1:1354176/1‑239 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTTATAAAAACCCACCACTTCTGCCACTTTTGCCCTTGCAGCCGGAAGGaaac                                                                                                                                                                      >  2:2195213/1‑238 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑AA‑GCGGAGGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                                      >  1:1086069/1‑239 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑AGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCCCTTCGGCCACTTTGGCCATTGCATCAGGAAGTaaaa                                                                                                                                                                      >  2:1605443/1‑239 (MQ=255)

                                                   aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑G‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                                      >  1:475238/1‑239 (MQ=255)

                                                     cGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAAGC‑TT‑AA‑GCGGATGGTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGa                                                                                                                                                                    >  1:953187/1‑239 (MQ=255)

                                                     cGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCAGGAAGAAAATC‑CT‑TA‑GCGGGTTTTT‑CCTTGCTTTATAGGCACCCGTCCCTTCGCTCACTTTGTCCATTTCCACATGAAGGaaaaaa                                                                                                                                                                    >  2:618718/1‑237 (MQ=255)

                                                      ggCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAGAGAAAATC‑TT‑AA‑GCGGATGTTT‑TGTTGCTTTATATACACCCCCCTCTCCCGGCACTTTGGCCCTTGCCGCCGGAAGTaaaaaa                                                                                                                                                                    >  2:816349/1‑236 (MQ=255)

                                                             cAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAACTCAAAAgg                                                                                                                                                            >  1:1002559/1‑239 (MQ=255)

                                                                  aacaCAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGGTGTTT‑CGCTGCTTTATAAAAACCCGCCCCTTCGGCCACCTTGGCCATTGCCGCAGGAAGGAAAAGATCAAAAGGGTTg                                                                                                                                                        >  2:1633250/1‑238 (MQ=255)

                                                                       aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTtat                                                                                                                                                                                                                      <  2:283159/171‑1 (MQ=255)

                                                                           aaTCAGCCCAAGAGAGAAGAAAATGCCGCCAACCATTTTTGCCATGCCGAGGTGTG‑TT‑GTG‑CCTG‑TGCCA‑TTGGTTGCTGTA‑ATATAGAAGACG‑AAGGCGATT‑GAAATAAAACCCCC‑GG‑CGG‑T‑AAT‑CGCAAAATAAAAAGTT‑TA‑AG‑GCGGTTGTTT‑CTTTGCTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTca                                                                                                                                              <  1:230955/239‑1 (MQ=255)

                                                                            aTCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcac                                                                                                                                             >  1:401259/1‑239 (MQ=255)

                                                                              cAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑ACGGATGTTT‑CCTTGCCTTATAGACACCCCCCCCTTCCGACAACTTGGCCAATGCCGCAGGAAGGTAAAGACCAAAAGGGTTGGCAGCTTTcaca                                                                                                                                            >  2:375210/1‑238 (MQ=255)

                                                                                gCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑AA‑GCGGGAGATT‑CGTTGCTTTATAGACAACCGCCTCTTCGGCCACTTTGGGCATTGCAGCCGGAAGTGAAAAATCAAAAAGGGTGTCAGCTTTCACACTa                                                                                                                                         >  1:2163977/1‑239 (MQ=255)

                                                                                     agagagAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAActc                                                                                                                                     >  1:1469938/1‑238 (MQ=255)

                                                                                     agagagAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTA‑TT‑AA‑GCGGATGTTT‑AGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTACAGCAGGAAGAAAAAGATCAAAAGGGTTGTAAGCTTTCACAATAActc                                                                                                                                     >  1:1270059/1‑238 (MQ=255)

                                                                                       ggagaAACAACTCCCGCCACCCATTTTGGCCATGCGGAGGGGCT‑TT‑GTG‑CCGG‑TGCCG‑GTGGTTGTTGTG‑TTAAAGAAGACG‑AAGGCATTG‑GAGATAAAACCACG‑GG‑CGG‑T‑ACC‑CGCAAGATAGAAAGTT‑TA‑AA‑GGGGGTTTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                                   <  2:614333/237‑1 (MQ=255)

                                                                                       agagaAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                                   >  1:1315914/1‑238 (MQ=255)

                                                                                        gagaAGCAAATGCCCCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTA‑ATATAGAAGACG‑AATGCGATT‑GAGATAAAACCCCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                                   <  1:961928/237‑1 (MQ=255)

                                                                                         agaAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTTACACTAActctctct                                                                                                                                >  1:1487393/1‑239 (MQ=255)

                                                                                          gaAGCAAAGGCCCCCAACCAGTTTTGCCAGGCCGAAGGGAA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGTTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACCCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGGTTGTTT‑CGTTGCTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTgcag                                                                                                                                                                                  <  1:1225478/188‑1 (MQ=255)

                                                                                           aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTTCTTTTTAGAAACCCCCCCCTTCGGCCCCTTTGGCCATTTCAGCAGGAAGTAAAAAAACAAAAAGGTTTGCCACTTTCACACCAACCCCCTCttt                                                                                                                              >  2:1576396/1‑239 (MQ=255)

                                                                                           aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAACATCAAAAGGGTTGTCAGCTTTCACACTAACTCCATCttt                                                                                                                              >  1:1126407/1‑239 (MQ=255)

                                                                                           aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑TGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACAATAACCTTCTCttt                                                                                                                              >  1:123795/1‑239 (MQ=255)

                                                                                           aaGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACCTTGGGCATTGCAGCAGGAAGTAAAAGAACAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCttt                                                                                                                              >  1:1077850/1‑239 (MQ=255)

                                                                                           aaGAAAATGCCGCAAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑GGCCA‑GTGGTTGCTGTG‑ATATAGAAGACA‑AATGCGATT‑GAGATAAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAAAGAAAGTT‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                                <  1:453257/189‑1 (MQ=255)

                                                                                              cAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTTTAGACACCCGCCCCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGTAAAAAAACAAAAGGGTTGTCAGCTTTCAACCTTACCCTCTCTttatt                                                                                                                           >  1:581271/1‑239 (MQ=255)

                                                                                                 cttcccccAACAGGTTTGGCCGTGCCGAGGGTTA‑GT‑GAG‑CCTG‑TGCTG‑GGTGTGGCGTTT‑ATATAGAAGATG‑AATGAGGAT‑GAGA‑GAAAACCCC‑GGTCGG‑A‑AAT‑CACCATGTAGAATCTT‑GA‑AA‑GGTTTTTTTT‑TGTTTTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTACCTCTCTCTTTATTAAg                                                                                                                        <  2:2059780/233‑1 (MQ=255)

                                                                                                 aTGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGGAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAg                                                                                                                        >  1:464319/1‑239 (MQ=255)

                                                                                                   gccgccAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTc                                                                                                                      >  1:542654/1‑239 (MQ=255)

                                                                                                   gccgccAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGACATTGCAGCCGGAAGGAAAAGATCAAAAGGGATGTCAGCTTTCACCCTAACTCCCTCTCTATTAAGTc                                                                                                                      >  1:1882603/1‑239 (MQ=255)

                                                                                                         accattttttGCGATGCCG‑AGGGGA‑TT‑GTG‑CCTG‑GGCCAGGTGGTTGGTGTA‑ATATAGAAGAGA‑AGTGCGATT‑GAGATAAAACCACC‑GG‑GGT‑T‑ACT‑CGCAAGATAAAAAGTT‑AT‑AA‑GCGTATTTTT‑TGTTTTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                                <  2:2182834/233‑1 (MQ=255)

                                                                                                                    ccATGCCGAAGGGTA‑‑T‑GTG‑GCTGCTGCGT‑GGGGTTGGTGTG‑ATATAGAAGACA‑AATGCGATT‑GAGAAGAAACCACC‑GG‑CGG‑T‑ACT‑CGCAAAATAGAAGGTC‑TA‑AG‑GCGGATGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                                      <  2:1061978/238‑1 (MQ=255)

                                                                                                                     aaTGCCGAAGGGCA‑TG‑GTG‑CCTG‑GGCCA‑GTGTTTGCTGTA‑ATATAAAAGACA‑AATGGAATT‑GAGAAAAAAACCCC‑GG‑CGT‑T‑AAT‑CGCCAGAAAGAAAGTC‑TA‑AA‑GGGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                                    <  1:1605443/238‑1 (MQ=255)

                                                                                                                      caggcggaGGGCA‑TTGGTG‑CTGG‑TG‑CA‑GGGGTTGCTGGGAATAAAGAAGAGA‑ATTGGGTTG‑GTGATAAAACCGCC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTT‑TT‑AG‑GGGGTTTTTT‑TTTTGTTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                                    <  2:740222/232‑1 (MQ=255)

                                                                                                                         cggAAGGGAA‑TT‑GTG‑CCTG‑TGCCA‑GGGGTTGCTGTG‑ATATAGAAGACA‑AATGCGATT‑GAGATAAAACCCCC‑GG‑CGT‑T‑AAC‑CGCAAGAAAGAAATTC‑TT‑AA‑GCGGTTGTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                                 <  1:703498/236‑1 (MQ=255)

                                                                                                                          tgaagggggttt‑GGG‑CCGG‑TGCAG‑GGGGTTGTTGTA‑ATAAAAAAGAGG‑AATG‑GATTAGAAAT‑AAAACCCC‑GG‑GGGAT‑AAC‑CGCAAAAAAAAAAGTT‑TA‑AA‑GGGGTTTTTT‑TGTTTTTTAAAGGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCACTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                                 <  2:534128/228‑1 (MQ=255)

                                                                                                                          cGAAGGGGA‑TT‑TGGCCCTG‑TGCGG‑GGGGTTGTGGAG‑ATAAAAAACACG‑AATGCGTTA‑GAGATAAAAAC‑CC‑GG‑GGG‑TAAAC‑CGCAAGATAGAAATTT‑TA‑AA‑GCGTTTTTTT‑CTTTTTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                                 <  2:1112368/238‑1 (MQ=255)

                                                                                                                                  a‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CCCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGGTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATAAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCCTTATTAAGTCGGCGGCGAGAAACTAACAAAACATTATAGAt                                                                                       >  2:852943/1‑239 (MQ=255)

                                                                                                                                         g‑cctg‑tgcaA‑GTGGTTGCTGTG‑AAAGAGAAGACG‑AATGCGTTG‑GAGATAAAAGCCCC‑GG‑CGG‑T‑AAT‑CGCAAGATAGAAAGTT‑TT‑AG‑GGGGATGTTT‑GGTTTCTTTAAAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATgaga                                                                                   <  1:501548/238‑1 (MQ=255)

                                                                                                                                            ctg‑tggcA‑GGTGTTGTTTGG‑ATAAAGAAGACG‑AATGATATT‑GAGATGAAACCCCC‑GG‑GGT‑T‑AAT‑CGCCAGAAAAAAATTC‑TT‑AA‑GTGTTTTTTT‑CGTTGCTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                                 <  2:464319/238‑1 (MQ=255)

                                                                                                                                            ctg‑tgcaA‑GTGTTTGCTGGG‑ATAAAGAAGACG‑AATGCTATT‑GAGATGAAACCCCC‑GG‑CGG‑T‑AAT‑CCCCAGAAAGAATGTC‑TT‑AG‑GCGGTTGTTT‑CGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                                 <  1:1576396/238‑1 (MQ=255)

                                                                                                                                             tg‑tggcA‑GTGGTGTCTGTG‑AAATAAAAGGAC‑AATGCGATT‑GAATGGAAACCACC‑GG‑AGT‑A‑ATT‑CGCTAGAAAGACAGTG‑AT‑AA‑GCGATTGTTT‑TGTCGCTATACAGCCCTCCGCCTCTTCGGCCACTTTGGCAATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAt                                                                                <  2:441526/238‑1 (MQ=255)

                                                                                                                                              tggtgcggggggTTTCTGAG‑AAAAAGAAGGCG‑AATGCGATT‑GGGAGAAATACCCC‑GG‑TGA‑T‑AAC‑CGCAAAATACAGAGTC‑TG‑AA‑GGGTGTGTTT‑CTTGTTTTTAGAGCCTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                                 <  2:1917039/228‑1 (MQ=255)

                                                                                                                                              g‑tgccA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGAAAGAAAGGC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTTCAGCAGGAAGGAAAAAATAAACAAGGTTGTCAGCTTTCACACTAACTCTCTCCTTATTAAGTCGGCGACGCGATACTAACAAAACATTATACATGAGAAATTg                                                                              >  1:1905052/1‑239 (MQ=255)

                                                                                                                                                tgccA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCCGGGAGTAAAAGATCCAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTTATTCGGCGACGTGATACTAACAACACATTATAGCTGAGCAATTGa                                                                             >  1:1156136/1‑239 (MQ=255)

                                                                                                                                                  ccA‑GTGTTTGGTGTG‑ATATA‑AAAACGAAAGGAGTTT‑GAAATAAAAACCCC‑GG‑CGG‑T‑AAC‑CGCCAGATA‑AAAGTT‑TT‑AAGGGGGTTGTTT‑GTTTGCTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGat                                                                            <  1:306231/238‑1 (MQ=255)

                                                                                                                                                    gtgTGGTTGCTGTA‑AAATAGGAGAGT‑AATGTGATT‑GAGA‑ACAACCACC‑GG‑ACG‑T‑AATCCCCCAGAT‑TAAAGTT‑TTGAA‑GCTGTTTTTT‑TGTTTCTTTAAAGACCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                           <  2:415760/236‑1 (MQ=255)

                                                                                                                                                    cattggttggtGGA‑ATAAAAAAGACA‑AGTGAGA‑T‑GAGAAGAAAACCCCGGG‑GGG‑T‑AAT‑CCCAAAATAAAAAGTT‑TA‑AG‑GCGGTTTTTT‑GTTATTTTTAAACAACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                           <  2:2139549/235‑1 (MQ=255)

                                                                                                                                                    a‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                                         >  1:1917039/1‑239 (MQ=255)

                                                                                                                                                       tGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGa                                                                       >  1:263906/1‑239 (MQ=255)

                                                                                                                                                        ggTTGCGGTG‑ATAAAGAAGACA‑AAGGGGTTT‑GAGATAAAAACACC‑GG‑CGG‑T‑AAT‑CGCAAAATAGAAAGTC‑TT‑AA‑GCGGTTTTTT‑CTTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                                      <  1:1633250/239‑1 (MQ=255)

                                                                                                                                                          ttggttgaaataaAGAAAACG‑AATGATATT‑GAGATAAAACCCCC‑GG‑GGT‑A‑AAC‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGTTTGTTT‑CGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                                      <  1:306429/229‑1 (MQ=255)

                                                                                                                                                           tGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGGTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCCGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCat                                                                   >  1:1085517/1‑239 (MQ=255)

                                                                                                                                                           tGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGAC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGTAAAAGATCAAAAAGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                                 >  1:32523/1‑209 (MQ=255)

                                                                                                                                                                   atatAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAATC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGATTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATACTAACAAAGCATTATAGATTAAAAAATGATATAGATCATAtctc                                                              >  1:1095547/1‑237 (MQ=255)

                                                                                                                                                                    taaagaagcCA‑AATGCAATT‑GATA‑GAAAACCCC‑GGTCTT‑T‑AAC‑CCCAAAATAAAAAGTC‑TA‑AA‑GCGGTTTTTT‑TTTTTTTTAAAACAACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                           <  1:852943/236‑1 (MQ=255)

                                                                                                                                                                         aagaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAAATAGAAAGTC‑TT‑AA‑ACGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACCCTAACTCTCTCTTTATTAAGTCGGCGACGAGAAACTAACAAAGCATTATAGATGAGAAATTGCTATAGATCATTTTCCGCCTGGCt                                                      >  2:746082/1‑239 (MQ=255)

                                                                                                                                                                           gaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGAGTTT‑TGTTGCCTTATAAACACCCGCCCCTTCGGCCAATTTTGCCATTGCAGCAGGAAGTAAAAAATCAAAAAGGGTGTCAGCTTTCACACTAACTCTCTCTTTTTTAAGTCCGCGACGAGATACTAACAAAACATTATAAATGAGAAATTTATATAGAACATTTCTCTCCTGGGTta                                                    >  2:1388847/1‑239 (MQ=255)

                                                                                                                                                                           gaCG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAATTTGatat                                                                          >  1:1127563/1‑217 (MQ=255)

                                                                                                                                                                              g‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctctct                                                                                                                                >  1:832973/1‑160 (MQ=255)

                                                                                                                                                                              aaaacgctttt‑GAGAAGAAACCCCC‑GG‑CGG‑T‑AAG‑CCCAATTTAGAACTTC‑TA‑AA‑GCGGTTGTCT‑CTTTTCTTTAAAGACTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTtata                                                  <  1:108216/230‑1 (MQ=255)

                                                                                                                                                                                aaTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGGTGTTT‑CGTTGCTTTATAGACACCCCCCTCTTCGGCCACTTTTGCCATTTCAGCAGGAAGTAAAAAATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACCAGACACTAACAAAACATTAAAGATGAGAAAATGATAAAGATCATAACTCGCCTTGCCTTTAgg                                                >  2:1690827/1‑239 (MQ=255)

                                                                                                                                                                                 aTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAATCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAACATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAg                                                 >  1:1300261/1‑237 (MQ=255)

                                                                                                                                                                                 aTGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAAAAAGTC‑TT‑TA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCCTCCGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGAAGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                            >  1:834429/1‑226 (MQ=255)

                                                                                                                                                                                      aTT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCgg                                                                                                                                                                                                    >  1:370741/1‑85 (MQ=255)

                                                                                                                                                                                           aaaaaaaaccccc‑GG‑CGA‑T‑AAT‑CGCAAGATAGAAATTC‑TG‑AG‑GTGTTTTTTT‑CTTGGCTTAAAAACCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACt                                       <  2:263906/227‑1 (MQ=255)

                                                                                                                                                                                                  aaCACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGGC‑TT‑AA‑GCGGAAGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGAAAAAGAt                                                                                                                                                                   >  1:1518326/1‑107 (MQ=255)

                                                                                                                                                                                                                  caT‑CGCAGGATAGAATTTC‑AG‑AA‑GCGTTTGTTT‑AGTTTCTTTACGGCCCCCCGCCTCTTCGGCCACTTTTGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGATAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAAt                   <  2:1151984/238‑1 (MQ=255)

                                                                                                                                                                                                                   aT‑CGCCAGATAGAAATTC‑TT‑AA‑GCGTTTTTTT‑CGTTGTTTAAAAGACAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa                  <  1:736017/239‑1 (MQ=255)

                                                                                                                                                                                                                   aT‑CGCACGATAAAAATTC‑TC‑AT‑GCGGTTTTTT‑TGTATCTTTAGATACACCCGCCTCTTCGGCCTCTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa                  <  1:947249/239‑1 (MQ=255)

                                                                                                                                                                                                                    agcGCCAGATA‑AAAATG‑TT‑AA‑GCGGGTGTTTCCTTTGCTTTAGAGTCAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa                  <  1:1421079/237‑1 (MQ=255)

                                                                                                                                                                                                                      cGCCAGATAGAATGTC‑TT‑AA‑GCGGTTTTTT‑TTTTTTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGACACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACg                <  1:1690827/239‑1 (MQ=255)

                                                                                                                                                                                                                        ccAGAGAGAAAGTT‑TT‑AA‑GCGTTTGTTT‑TGTTGATTTAGAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGa              <  1:746082/239‑1 (MQ=255)

                                                                                                                                                                                                                         cAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAg             >  1:979501/1‑239 (MQ=255)

                                                                                                                                                                                                                          agaaagaaCGTC‑TA‑AA‑GCGTATGTTT‑GGTTTCTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt            <  1:618718/239‑1 (MQ=255)

                                                                                                                                                                                                                          aGATAAAAATTT‑TT‑AC‑GCGGTTTTTT‑CGTTTATCTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt            <  1:1388847/239‑1 (MQ=255)

                                                                                                                                                                                                                             gaGAAATTC‑TT‑GA‑GGGGTTTTTT‑CTTGGTTTTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt            <  1:714882/235‑1 (MQ=255)

                                                                                                                                                                                                                                        t‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGa  >  1:849456/1‑236 (MQ=255)

                                                                                                                                                                                                                                                 aTGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa                                                                                                                         >  1:915217/1‑110 (MQ=255)

GATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCA‑TT‑GTG‑CCTG‑TGCCA‑GTGGTTGCTGTG‑ATATAGAAGACG‑AATGCGATT‑GAGATGAAAACACC‑GG‑CGG‑T‑AAT‑CGCCAGATAGAAAGTC‑TT‑AA‑GCGGATGTTT‑CGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGA  >  NC_007779/954574‑955027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`