| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_007779 | 990,324 | C→T | 17.9% | S152N (AGC→AAC) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 990,324 | 0 | C | T | 17.9% | 145.7 / 9.9 | 75 | S152N (AGC→AAC) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (19/40); new base T (5/8); total (27/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCA > NC_007779/990088‑990562 | ggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAAAGCCGCGAAGTCGACG‑AATTTTC‑tt > 1:1240822/1‑239 (MQ=255) tGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTcc < 2:1161684/239‑1 (MQ=255) tttGCTGATGTGCGTGGAACCGTTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGGCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATGCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGATTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑c < 2:555733/239‑1 (MQ=255) ttGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGATAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑Cg < 2:546321/239‑1 (MQ=255) gATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCg < 2:1252126/239‑1 (MQ=255) gCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGc < 1:87023/239‑1 (MQ=255) ccTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAAAGCCGAGAAAACGAATAAATTTGT‑TTTCCA‑‑CGTGTCGgggggt > 1:1351703/1‑237 (MQ=255) attctggtgCCTGGGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACg < 2:825836/233‑1 (MQ=255) aTTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGGGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGCTAACGGCGGGAAGACGTCT‑AATTTTT‑TTTCCAG‑CGGGTCGGCGGGGGGC‑GC‑AACg > 1:441559/1‑239 (MQ=255) ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCCGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTGAACACCCGGAAATAGACC‑AATATTC‑TTTCCCT‑AGTGTGGAGGGCGTTC‑GC‑aaac > 2:304979/1‑236 (MQ=255) ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCCCGGAGGCGAAT‑AATTTGG‑TTTCCAG‑CGTTTTGGGGGGGTGG‑GC‑AACGt > 1:2098918/1‑239 (MQ=255) ttCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCCAGCAGAGCGCC‑AAATTGC‑TTTCCCG‑CGCGTTGGGGGGCTGC‑GC‑aaag > 1:1089889/1‑236 (MQ=255) gtgCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGc < 1:952121/238‑1 (MQ=255) gtgCCTGTGTTCCCATCGGCGTGAGGGAAAGACGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGc < 2:2030530/238‑1 (MQ=255) tgtgTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCGAGAAGTCGGCG‑AATTTGT‑TTTTCAC‑CGTGTCGGGGGCTTGC‑GC‑GACGT‑CCGCCTCCgg > 1:1222456/1‑239 (MQ=255) gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTg < 1:1059553/238‑1 (MQ=255) gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTg < 1:1387386/238‑1 (MQ=255) tCCCCTCGGCGTGAAGGAAAGCGGGCGCGCCGGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTGGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGTCCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGag < 2:1227458/238‑1 (MQ=255) tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGag < 1:948646/238‑1 (MQ=255) aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCGCGAAAGCGACT‑AATTTTT‑TTTTCAG‑CGTGGCGGGGGCGTGC‑GC‑CACGT‑CGGCCTGCGGGGagggata > 1:514735/1‑239 (MQ=255) tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatag < 1:967367/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCTAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 1:1867622/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 2:733671/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 1:156272/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCGGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 1:126229/239‑1 (MQ=255) ggCGTGAAGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 1:1120748/239‑1 (MQ=255) gCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGGCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTTTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTg < 1:1916812/228‑1 (MQ=255) gCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TGTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGagcgatagcg < 2:1232274/238‑1 (MQ=255) cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAAAGCCGCGAAGGAGACT‑CATTTGC‑TTTTCAGACGGGT‑GGGGGGGTGC‑GC‑CACGG‑CGGGCTGCGGGGggcggtagagat > 1:723279/1‑239 (MQ=255) gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACACACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATg < 1:966238/239‑1 (MQ=255) gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATg < 1:1048705/239‑1 (MQ=255) gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATg < 1:1392710/239‑1 (MQ=255) gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAAGCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATg < 1:127355/239‑1 (MQ=255) gAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTa < 1:168327/239‑1 (MQ=255) cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑CACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAt > 1:1992816/1‑239 (MQ=255) cAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTa < 1:1743788/221‑1 (MQ=255) aGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCCAAAATATGCAT‑AATTTTC‑CTTTCAG‑CGGGTCGAGGGCCGTC‑GC‑AACCT‑CGGCCTTGGGGTAGCGTGAGCGATGTACCATTTCACTGACaaact > 2:195094/1‑235 (MQ=255) acaAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGAGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAAAGCACAGAAGTCGACT‑AAATTGC‑TTTCCAG‑CGTGTCGAGGGCGTGC‑GC‑AACGT‑CGGCCTGCGGGGGGCGATAGCGATGTACCACTTCTCTGATAAACGCCCGCAAAGGAACCTCCCagag > 1:160297/1‑239 (MQ=255) aaaCCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAAAGCCGAGAAGTGGACT‑AATTTGG‑TTTTCAG‑CGTGGCGAGGGGTTGC‑GCGAACGT‑‑GGCCCGCGGTGAGGGAGAGAGCAGTACCACTTCTCTGAAAAACCCCAGCCAAGGAACCTTCCCGAGGa > 2:1387799/1‑239 (MQ=255) aTTCCTGTGGCGGCCGCTAAACGGTGGTTTCTTGGCCTCCGGGGAAAAACCGGCGCGGGGGGCAAAAACCCGTCGGATGGAGGCGCAAAAAATAA‑AGCCCGGCGGTTACCGCCGAGAAGGCGATT‑AATTGGC‑TTTCCGG‑GGGGTGGGGGGCTTGC‑GC‑AGCGT‑CGGCCTGGGGTGGGCGATAGAGGTGTCCCTTTCCACGGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTc < 2:1222456/238‑1 (MQ=255) cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGGCGACT‑AATTTTC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAAACGCCCGCAAAAGaa > 1:1317638/1‑215 (MQ=255) gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCCAGAAGTCGACT‑AATTTGC‑TTTTCAG‑CGGGTCGAGGGCTTTC‑GC‑AACGT‑CGGCCTGCGGGGAGCGATAGCGATGTACCCTTTTACTGATAACCGCCAGCCAAAGAACTTCCCCGAGGATGACTTTCCGccc > 1:1353043/1‑237 (MQ=255) gCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCtt > 1:88441/1‑239 (MQ=255) aaCGATGGTTTCTTGTGCTCCGGGA‑AAAAACGGGGGCGGGGGCCAAAAACCAGCGGAAGGGAGGGGCGCATGTCAGACACGGGCGTTTACCGCGGAGAGGGCGGCT‑AATTGGT‑TTCCCGG‑TGTG‑GGGGGGGTTGCGGC‑AGCGT‑CGGCCGGCGGGGAGGGAGAGCGTGCTCCGATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAAc < 2:277131/239‑1 (MQ=255) tGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGGCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGG‑CGGCCTGCGGGGAGCGATAGCGATGTACCCTTTCACTGATAACCGCCAGCAAAGGaa > 1:1764203/1‑195 (MQ=255) gTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑GGACCGGGGGTTAACGCCGAGAAGTCGCCT‑AATTTGC‑TTTCCAG‑CGTGTGGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCTTTTCACTGATAACCGCCAGCAAAgg < 1:385115/191‑1 (MQ=255) tttCTTGTACTTCGCGAGAAAAACGGCGACGGGTGTCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAAAGCCGAGAAAGCGACC‑AATTTGA‑TTTTCAG‑CGTGTCGGGGGGTTGC‑GC‑AACCG‑CGGCCTGCGGGGGGGGAGAGCGATGTACCATTTTACTGATAACCGCCCGCAAAAGGACATTCCCGAGGAGGGAATCTCCCCACGGGCCGCCTTAACAAatcgcc > 2:543117/1‑235 (MQ=255) tCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGGTAACGCCCAGAAGTCGACT‑CATTTGT‑TTTCCAG‑CGTGTCGAGGGGATGC‑CC‑AACAT‑CGCCCCCCGGCGGGCGATCGCGATGGATCGTTTCTCTTATAACCGCCCGCCAACGAAGTTCCCAGAGGGTGAATTCACACCCCTCACCGCCTTAACCAATATcac > 2:1777142/1‑236 (MQ=255) cTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCTTCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTTACGCCCAGCAATCGACT‑AAATTTC‑TTTCCAC‑CGTGTCGAGGGGCTGC‑GC‑CACGT‑CGGCCTGCGGGGAGCGCTAGCGATGTACCATTTCTCTCATAACCGCCAGCAAAGGGACATCCCCGAGGGTGACTTTACGCCCCAGGGCCCGTAAACGAAAATcccacctgcc > 2:554552/1‑230 (MQ=255) ttCGCGAGAAAACCGGCGACGGGTGCAAAAATCCTTCAGAGGGAAGCGCGAAATACCA‑AGCCCGTCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTCCCAG‑CGTGTCGAGGGTTTGC‑GA‑AACGT‑CGGCCTGCGGGGAGAGATAGCGATGTACCTTTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt < 2:1353043/239‑1 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCCAGAAGTCGAAT‑AATTTTC‑TTTTCCG‑CGCGGCGAGGGGCTGC‑GC‑CACGT‑CGGCCTGCGGGGGGGGGTAGCGGTGGTACATTTCACTGGTAACCGCCCGCAAAGGGACTTCCCCGAGGATGACTTCACCCCACGGGCCGCCTAAACAAAAATCCACCCTGCCATTa > 2:156904/1‑239 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCCAGAAATCGAAT‑AATTTTC‑TTTCCCA‑CCTGTCGAGGGGGTGC‑CC‑AACGT‑CGGCCTGCCGGGAGCGATAGCGATGTAACATTTCACTGATAACCGCCCGCAAAGGAACTCCCCCGAGGGTGACTTCACGCCCCGGGCCCCCTACACGAAAATACAACCTGcgcttt > 2:731884/1‑234 (MQ=255) gaAAAACGGCGAGGGGTGCCAAAGCCCATCAAAGGGAAGCGCTGAACAACA‑AGCCGGGGGTTTCTCGCCGGGAAGCCGCAT‑ATTTTGT‑TTGCCGG‑AGGGAGGGGGGTTTGC‑‑C‑ACTTTCCGGCCGGGGGGGGGCAATGCGGGTGAACCTTTTCATTGAAATCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCg < 2:1539586/237‑1 (MQ=255) cGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGCCCGGCGGTTACCGCCGAGAAGTCGACT‑ATTTTGC‑TTCCCAG‑CGTGTCGAGGGCTTGC‑GC‑ACCGT‑CGGCCGGCGGTGAGCGATAGCGATGTCCCATTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGAc < 1:156904/239‑1 (MQ=255) ccAAAATCCATGAGGTGGAACGGCGACATATCA‑GGCCCGGCGGTTCAGGCCGAGAAGTCGTCT‑AATTTGC‑TTTCGCG‑CGTGTCGAGGCCTGGC‑GC‑AACGG‑CGGCCTGGGGGGAGCGATAGAGATGTACGATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTc < 2:1850461/239‑1 (MQ=255) tCCCTCAGAGGGAAGCGCGAAATATCA‑AGACCGGCGGTTAACGCGGAGAAGTCGCCT‑AATTTGT‑TTTCCAG‑CGTGTGGAGGGCTTGC‑GC‑AACGT‑CGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGtt < 1:731884/239‑1 (MQ=255) ggcgcGAATTATCA‑AGCCCGGCGGTTAACGCGGAGAGGTCGACT‑AATTTGT‑TTTCCAG‑CGTGTCGAGGGTTGGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCTTTTCACGGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTc < 2:155283/156‑1 (MQ=255) gataaacga‑aGACCGGCGTTTACGGCGGAAGAGCCCATT‑ATTTTGT‑TTCCCGG‑CGTGTCGAGGGTTGGC‑GA‑AACGT‑CGGCCGGCGGTAGGCTATGGCGATGACCCATTTCACAGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 2:723279/231‑1 (MQ=255) gaaatatCA‑AGCCCGGGGGTGAACGCCAAGAGGTCGACT‑ATTTTGT‑TTCGCGG‑CGTGTGGAGGGCTTGC‑GC‑AACGT‑CGGCCGGCGGGGAGCGATAGCGATGTCCCATTCCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 2:1587940/236‑1 (MQ=255) gACATACCA‑GGACGGGCGGTTACGGCGGAGAAGCCGATT‑AATTTGC‑TTTCCAG‑GGTGTCGAGGGTTTGC‑GC‑AACGT‑CGGCCTGCGGTGGGGGATGGCGATGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:543117/239‑1 (MQ=255) ataatCG‑AGACCGGCGGGTAACGGCGAGAGTGCGACT‑ATTTGGT‑TTTCCAG‑CGTGTGGAGTTCTTGC‑GA‑GACGT‑CGGCCTGGGGTGAGAGATAGCGATGCACCATTTCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAcc < 2:160297/236‑1 (MQ=255) atCA‑AGACCGGCGGTTAACGCCGAGAAGTCGGCT‑AATTTTC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑CACGT‑CGGCCTGCGGGGAGCGATAGCGATGTAACCTTTCACTGATAACCCCCAGCCAAAGAACTTCCCCGAGGGTGACTTTACGcccccgg > 1:155283/1‑152 (MQ=255) atCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGTTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa < 1:1869731/187‑1 (MQ=255) ggcggTAAACGCCGAGAAGTCGACT‑ATTGTGC‑TTTCCGG‑GGTGTCGCGGGCTTGC‑GC‑AACGT‑GGCCCGGCGGGGAGCGATAGCGATGTACCATTTCACTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:1656320/239‑1 (MQ=255) ggggtaaccGCCGAGAGGGCGCTT‑ATTTGGC‑TTCCCAG‑CGTGTCGAGGGCTTGC‑GA‑AACGT‑CGGCCTGGGGTGAGCGATAGCGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:506449/230‑1 (MQ=255) gcgtTTAGCGCCGAGATGT‑GACC‑TATTTGCGTTTCCAG‑GGTGTCGGGGGCTTGC‑GC‑AACGT‑CGGCATGGGGGGGGCGGTAGCGATGTACCATTTCCCTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:1240822/238‑1 (MQ=255) gcggTTAACGCGGAGAAGTCGACT‑ATTTTGC‑TTCCCGG‑GGTGTCGAGGGTTTGC‑GC‑AACGT‑GGGCCTGGGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:1759609/238‑1 (MQ=255) gaccGCCGAGATCTGGACT‑AATTGGC‑TTCCCAG‑CGTGTCGGGGGCTTGC‑CC‑AACGT‑GGGCCTGCGGAGAGCAATAGCGATGCACCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt < 1:1732736/236‑1 (MQ=255) gaaCGCCGAAAAGTGGACT‑ATTTTTC‑TTTCCAG‑CGTGTGGAGGGTTTGC‑GC‑AACGT‑CGGCCGGCGGTGAGCGATAGCGGTGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt < 1:304979/238‑1 (MQ=255) cGAGATGTCGTGT‑ATTTTGC‑TCCCCAG‑CGTGTGGCGGGCTTGC‑GA‑AACGC‑CGGCCGGGGGTGAGCATTAGCGATGTACCTTTTCATTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGGACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag < 2:514735/238‑1 (MQ=255) agaAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa > 1:37923/1‑239 (MQ=255) tCGATT‑AATTTGT‑TTTCCGC‑GGTGTCGAGGGTTGGC‑GA‑AACCT‑CGGCCTGCGGTGAGGAATTGCGATGTCCCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca < 1:1387799/238‑1 (MQ=255) tCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag > 1:302642/1‑239 (MQ=255) cGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCGTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgccagg > 2:2055140/1‑239 (MQ=255) tGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg > 1:62272/1‑238 (MQ=255) c‑TTTCCAG‑CGTTTGGAGGGCTTGC‑GC‑AACGG‑CTGCCTGGGGTGGGCGATAGCGATGTCCCTTTTCCGTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa < 1:1620775/239‑1 (MQ=255) c‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc > 1:219619/1‑238 (MQ=255) | GGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCA‑AGACCGGCGGTTAACGCCGAGAAGTCGACT‑AATTTGC‑TTTCCAG‑CGTGTCGAGGGCTTGC‑GC‑AACGT‑CGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCA > NC_007779/990088‑990562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |