BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	1,077,794	T→G	9.6%	Y504S (TAC→TCC)	putA ←	trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,077,794	0	T	G	9.6%	483.9 / 3.1	210	Y504S (TAC→TCC)	putA	trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (95/83); new base G (25/5); total (121/89)
Fisher's exact test for biased strand distribution p-value = 2.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

TCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGA  >  NC_007779/1077557‑1078030

tCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGgt                                                                                                                                                                                                                                                <  1:836362/238‑1 (MQ=255)

    cGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CAGTGGAAATCT‑TAATGACCCGGGtag‑cag                                                                                                                                                                                                                                          >  1:477870/1‑239 (MQ=255)

    cGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑ACATGCAGG‑CACTGGAAACCG‑TACTGACCCGGggagggg                                                                                                                                                                                                                                           >  1:1701724/1‑236 (MQ=255)

                ccAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGCTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGAACTGC‑TCATACAGTGGCTCGCCCATA‑ACATTCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGcc                                                                                                                                                                                                                              >  1:1082848/1‑239 (MQ=255)

                    tGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCCTGACGG‑CCCTGGAACTCG‑TACTGTCCCGGGGCG‑TAGTCTTTCCCCGCCac                                                                                                                                                                                                                            >  2:444391/1‑236 (MQ=255)

                              gtgtCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGACGACGCAACAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑CAGTTCTGCCCCCCCAGTTGATAAATcg                                                                                                                                                                                                                 >  1:738536/1‑238 (MQ=255)

                                 tCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATcgccg                                                                                                                                                                                                              >  1:1503709/1‑238 (MQ=255)

                                  cGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGCAG‑TAGTTCTGCCCCGCCAGTTGATAAATcgccgc                                                                                                                                                                                                             <  1:1496685/238‑1 (MQ=255)

                                   ggCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCa                                                                                                                                                                                                           <  1:444391/239‑1 (MQ=255)

                                   ggCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCa                                                                                                                                                                                                           <  1:1334008/239‑1 (MQ=255)

                                      aaTACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCCTA‑CCATGCAGG‑CCCTGGAACTCG‑TACTGCCCCGGGGTG‑TCGATCCTCCCCCCCCGTCGAGAAATCGCCCCCacc                                                                                                                                                                                                         >  2:2127355/1‑236 (MQ=255)

                                         aCGGTTAACAACCGGGGGGTGAGCACCGTTTTCCAGCAGGCGACGCCCCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGCGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGGCAGTTGATAAATCGCCGCCAGCGTAt                                                                                                                                                                                                     <  2:1638328/239‑1 (MQ=255)

                                         aCGGTTAACAAACTAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATCCGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGGTCGCCCATA‑CCATGCAGG‑CCCTGGAACTCG‑TACTGTCCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTAt                                                                                                                                                                                                     <  2:910539/239‑1 (MQ=255)

                                         aCGGTTAACAAACGAGTTGTTGGCACCGTTTTCCAGCAGGGGACGCACCAGATGCGCCAACGGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGGC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTAACCTGC‑TCATACGGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TCCTGCCCCGGGTAG‑TAGTACTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTAt                                                                                                                                                                                                     <  2:536214/239‑1 (MQ=255)

                                         aCGGTTAACAAACGAGTTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCGAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCACCTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGGCCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTAt                                                                                                                                                                                                     <  1:2061169/239‑1 (MQ=255)

                                            tgtagcAAACGAGGTGTTGGAACCGGTTACCAGCGGGTAACGCACCGGATACGCCGACGGCTGTTCATGTGT‑GCACACGGGGGCATAAATACGAGCCGGAG‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACTTGC‑TCATCCAGGGGCTCGCCCATA‑CCACGCAGG‑CACTGGAACTTG‑TCCTGACCCGGCTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATgg                                                                                                                                                                                                   <  2:1160593/233‑1 (MQ=255)

                                              tAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCCGGTAG‑TAGTTCTGCCCCGCCAGCTTATAAATTGCCGCCAGCGCATGGGCg                                                                                                                                                                                                >  1:1126142/1‑239 (MQ=255)

                                               aaCAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGgtag                                                                                                                                                                                                                                              >  1:256477/1‑193 (MQ=255)

                                               aaCAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGgtag                                                                                                                                                                                                                                              <  2:256477/193‑1 (MQ=255)

                                               aaCAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGt                                                                                                                                                                                               >  1:1138053/1‑239 (MQ=255)

                                                     cGAGGTGTTAACACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGGCCCTA‑CCACGCAGG‑CACCGGAACTCG‑TACTTAACCGGGTAG‑TAGTTCTGCCCCGCCCGTTGCTAAATTGCCGCCCGCGGAAGGGGGtttgtgc                                                                                                                                                                                         >  2:230959/1‑234 (MQ=255)

                                                       aGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACCGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGCTGATAAATCGCCGCCAGCGCATGGGCGCTGTGCgg                                                                                                                                                                                        >  2:2186986/1‑237 (MQ=255)

                                                       aGGTGCTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACCGGAACTCC‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCCGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTc                                                                                                                                                                                       >  1:971086/1‑239 (MQ=255)

                                                          tgtTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATT‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTcg                                                                                                                                                                                      <  1:393493/237‑1 (MQ=255)

                                                          tgtTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑CAGTTCTGCCCCGCCAGTTGATAAAACGCCGCCAGCGTATGGGCGTTGTGCg                                                                                                                                                                                         >  1:992683/1‑234 (MQ=255)

                                                           gtTGGCACCGTTTTCCAGACGGCGACGCACCAGATACGCAACCAGCGTTTCATGGGT‑GCCAACCGGGGCTTAAATACGCCACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGGGACCTGC‑TCATCCAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TCCTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGa                                                                                                                                                                                   <  2:425403/239‑1 (MQ=255)

                                                              cgCACCGTTTTTCCGCAGGCGACGCACCAAGCACCCCAACAGCTTTTCAT‑TGTGGCCAACCGGAGCAAAAATACGAACCCGAG‑CGTTAAGTTTTGCGTCGGCAAGCTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCAGGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACt                                                                                                                                                                                <  2:47757/238‑1 (MQ=255)

                                                              aGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCCTA‑CCATGCAGG‑CAATGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGa                                                                                                                                                                                   >  1:271567/1‑236 (MQ=255)

                                                                    gTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGGGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGt                                                                                                                                                                          >  1:2084380/1‑239 (MQ=255)

                                                                    gTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGgta                                                                                                                                                                                                                                               <  1:810966/171‑1 (MQ=255)

                                                                                gCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGAGAAATCcccgcc                                                                                                                                                                                                            >  2:13861/1‑193 (MQ=255)

                                                                                   aCGCGCCAGATCCGCCACAGGCGTTCCATGTGC‑GCCAACCGGGGCAGAACACCGCGCCGGAT‑GTTTAAGTGTGCCGTCGGCAACTTCCCG‑GGTGTCCGGC‑TCATCCAGGTGCTCGCCCGTA‑CCAGGCGGG‑CCTTCGAGCTGT‑TCCTGACCTGGGTAG‑TGTTTCGGCCCCGCCAGTTGATAAATCGCACCGAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTGGATTCGGc                                                                                                                                                            <  2:1619838/238‑1 (MQ=255)

                                                                                              aCGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGGAGATTAGATTCGGCACCGCcagca                                                                                                                                                  >  1:1260798/1‑237 (MQ=255)

                                                                                                        cGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCACCGTATGGGCGTTGGGCGTCGCGCACTGCGGGGAGCTTAGCTTCGGCACCGCCAGCGGCTTTTTCGcg                                                                                                                                       >  1:1539973/1‑237 (MQ=255)

                                                                                                          tttCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCCCAg                                                                                                                                    >  1:1433041/1‑239 (MQ=255)

                                                                                                          tttCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAg                                                                                                                                    >  1:1319622/1‑239 (MQ=255)

                                                                                                          tttCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAg                                                                                                                                    >  1:404095/1‑239 (MQ=255)

                                                                                                           ttCATGGGT‑GCCAACGGGACCATAAATGCGCACCGGAC‑GTTTATGTTGCCCGTCGGAAACTTTCCG‑GGTGACCGTC‑TCATACGGTGGCTCGCCCTAC‑CCATGCGGG‑CACTGGAACTCT‑TACTGACCGGGGTGG‑TGTTTCGGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAgg                                                                                                                                   <  2:1260798/239‑1 (MQ=255)

                                                                                                           gtCATGTGG‑CCCCACCGGAGCAACAATACGACCCGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATCAAGTGGCGCGCCCATA‑CCAGGCAGG‑CACTGAAACCCG‑TCCTGCCCGGGGTGG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACGGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAgg                                                                                                                                   <  2:2077013/238‑1 (MQ=255)

                                                                                                                         aCCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGAAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCCCCAGGGTATGGGCGTTGTGCGTCGCGAACCGCGGGTAGATTAGATTCGACACCGCCAGCAGGTTTTTCGCACAGGCGAGATAAGaaa                                                                                                                       >  1:1627463/1‑238 (MQ=255)

                                                                                                                          ccGGAGCATAAATACGACACGGGC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  1:1202793/239‑1 (MQ=255)

                                                                                                                          ccGGAGCATAAATACGACACGGAC‑GTTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATC‑CCAGGCAGG‑CACTGGAACTCG‑TCCTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  1:182254/239‑1 (MQ=255)

                                                                                                                          ccGGAGCATAAATACGACACGGAC‑GGTTAATTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  1:521088/239‑1 (MQ=255)

                                                                                                                           ccgaccATATATACGACACGACA‑GTTTAATGTTGCGGGCCGCACTTTTCCC‑GGTGCCCTGC‑TCATACATGGGTCCGCCCAT‑‑CCATGCCGGTCTCGGAAACTCT‑CACGGTCCTGGGTGG‑TTGTTCTGCCCCGCCAGTTGGTAAATTGCCGCAGGCGTATGGGCGTTGTTCCTCGAGAACTGCGGGTAGATTATATTGGGCTCCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  2:467148/233‑1 (MQ=255)

                                                                                                                                taaaaagacaccacGGCG‑GGTAAAGTTTCCCGTCGGCACTTTCCCC‑GGTGACCTGC‑TCATACAGGGGCTCGCCCTTA‑CCAGGCAGC‑CACGGAACCCCG‑TACTGA‑CCGGGTAGTTATTTCTGCCCCGCCGGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGta                                                                                                              <  2:2151245/228‑1 (MQ=255)

                                                                                                                                         gACACGGCC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTg                                                                                                     <  1:722252/239‑1 (MQ=255)

                                                                                                                                          gcacGGACGGGTTAAGGTTGCCGCCGACACCTTTCCC‑GGGGACCTGC‑TCATACAGTGGGTCGCCCCTC‑CCAGGCAGG‑CACGGGAATGGG‑TACTGCCCGGGGTGG‑TGGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGGGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTg                                                                                                     <  2:2218381/238‑1 (MQ=255)

                                                                                                                                          acacGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCT‑GGTGACCTGC‑TCATACAGTGGCTCGCCCCTA‑CCATTCAGG‑CACTGGAAATCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCCCCCGCGTATAGGCGTTGTGCGTCGCGAACTGCGGGGAGATTACATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATGAGAAAACTC‑GGGATACACCCTGc                                                                                                    >  2:568011/1‑239 (MQ=255)

                                                                                                                                          acacGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGc                                                                                                    >  1:2222204/1‑239 (MQ=255)

                                                                                                                                          acacGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGGTGATAAATCTCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATTAGAAACGGC‑GGTATacacactgc                                                                                                    >  1:1929517/1‑234 (MQ=255)

                                                                                                                                          aaccgtaggggTTACGTTAGCCGTGGGCATCTTTCCC‑GTGTACTTCC‑TAATACGGGGGCTGCCCCTTC‑CCACACAGG‑CAGTGAAACTGT‑TACTGACCGGGGTAT‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCGGCGTATGGGCGTTGTGCTTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTg                                                                                                     <  2:971086/230‑1 (MQ=255)

                                                                                                                                                ac‑ggTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGcc                                                                                                                                                                                                                              >  1:1238071/1‑112 (MQ=255)

                                                                                                                                                ac‑ggTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCgag                                                                                                                               >  1:361522/1‑207 (MQ=255)

                                                                                                                                                ac‑ggTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAAACGCCGCCCGCGGATGGGCGGTGTGCGTCGCGAACTGCGGGTAGCTTAGATTCGGCACCGCCAGCAGCCTTTTCGCACCAGCGAGAAAAGAAAATTC‑TGAATACCACTTGCGGGtgt                                                                                              >  2:655463/1‑237 (MQ=255)

                                                                                                                                                   gacggaGTTTCCCCTCGGC‑ACTTTCCCGGGTAACCGGC‑ACTTCCGGGGGTGCCCCCATC‑CCATCCAGG‑CCTGGGACCTGG‑TACCGCCCGGGGTAG‑TATTCCTGCCCCGCCAGTTGATAAATCGCCGCCGGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGtata                                                                                             <  2:825075/233‑1 (MQ=255)

                                                                                                                                                     ttATGTTTGCGGCCGGCAACTGTCCC‑GGTGACCTGC‑TCATCCAGGGGCCCGCCCATC‑CCATGCGGC‑CAGTGGAACGCG‑TACTGACCGGGGTAG‑TGGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATaaa                                                                                           <  2:1106252/238‑1 (MQ=255)

                                                                                                                                                     ttAAGTTTGCCGTCGGCAACTTTCCC‑GGTGCCCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TCCTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATaaa                                                                                           <  1:655463/238‑1 (MQ=255)

                                                                                                                                                     tgaggTTGGCGGTGGGCACCTTCCCG‑GGTGCCCCGC‑CCACACAGGGGCGCGCCCTTA‑CCAGCCAGG‑GAGTGGAACCCG‑TGCTGGCCGGGGGGG‑TAGTTCTGCCCCGCCATTTGATAAATCGCCGCCAGCGTATGGGCGTTGGGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATaaa                                                                                           <  2:1309331/234‑1 (MQ=255)

                                                                                                                                                     tgaaGTTTGCCGTCGGCACCTTTCCC‑GG‑GATCTGCTTCACACGGTGGCTCGCCCATA‑CCTTGCAGG‑‑ACCTGAAATCGTTCCGGACCGGGTTGG‑TAGTTCCGCCCCGCCGGTTGATAAATCGCCGCCAGCGGATGGGCGTTGGGCGTCGCGACCTGCGGGTAGATTAGATTCGGCACCGCAAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAAc                                                                                          <  2:1929517/237‑1 (MQ=255)

                                                                                                                                                             gCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATaa                                                                                   >  1:1444118/1‑238 (MQ=255)

                                                                                                                                                                   ggCAACTTTCCC‑GGTGCCCTGC‑TCATACAGTGGCTCGCCCATC‑CCATGCAGG‑CACTGGAACTCG‑TACTGCCCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCaa                                                                            <  1:1408680/239‑1 (MQ=255)

                                                                                                                                                                   ggCAACTGTCCC‑GTTGACCGGC‑TCATCCAGTGGTTC‑CCCATACCCATCCGGG‑ACTTGGAACTCG‑TCCGGGCCGGGGGGG‑TGTTCCGGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTGGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCaa                                                                            <  2:1136637/239‑1 (MQ=255)

                                                                                                                                                                                     ccTGC‑TCATACAGTGGCTCGCCCATC‑CCATGCGGG‑CATTGGACCTCG‑TCCTGACCGGGTTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCTTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTgcgc                                                            <  1:577529/238‑1 (MQ=255)

                                                                                                                                                                                     ccTGC‑TCATACAGTGGCTCGCCCATC‑CCATGCAGG‑CACGGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTgcgc                                                            <  1:236743/238‑1 (MQ=255)

                                                                                                                                                                                         ctccATACCGGGGCTGCCCCATA‑CCAGGCAGG‑CAGGGGAACTGT‑GCCTGCCCCGGGGGG‑TAGTTGTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTCGGGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACg                                                         <  2:743111/236‑1 (MQ=255)

                                                                                                                                                                                         c‑TCATACGGGGGCTCCCCCATC‑CCATGCGGG‑CACTGGAACTCG‑TCCTGCCCCGGGTGG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  2:304339/178‑1 (MQ=255)

                                                                                                                                                                                               aCAGTGGCTCGCCCATC‑CCATGCAGG‑CACTGGAACTCG‑TACTGCCCCGGGTAT‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCAt                                                                  <  1:773304/223‑1 (MQ=255)

                                                                                                                                                                                                  gTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATa                                                                                    <  1:193496/202‑1 (MQ=255)

                                                                                                                                                                                                  gTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATa                                                                                    >  2:193496/1‑202 (MQ=255)

                                                                                                                                                                                                   tGGCTCGCCCATC‑CCATGAGGG‑CACGGGAACCCG‑CACTGACCGGGGTAG‑TAGTTTGGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCa                                               <  1:1728059/238‑1 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCCGTTGATAAATCGCCGCCAGCGGATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCTGCACCGCCAGCCGCTTTTTCGCACAGGCGAGATAAGAAAAGGC‑GGTATACACCCTTCGGGTATAAACCGGATAAACTTCAAAGCCGGCCCACCGCGCCCGCCTACTTTCACt                                             >  2:1728059/1‑239 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCCGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACCGGCGAGATAAGAAACGTC‑GGTATaca                                                                                                          >  1:304339/1‑178 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCCCCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATAAACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCAGCTGCGCACGCTTAATTTCACt                                             >  1:644640/1‑239 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAAACTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACt                                             >  1:1907043/1‑239 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGACAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACt                                             >  1:214379/1‑239 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCCGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAAAGTC‑GGTAAACACCTTGCGGGTATAAACCGGATAAACTTCAAGGCCGCCCATCTGCGCACGCTTTAATTCACt                                             >  2:1552454/1‑239 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCCCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGGAGATTAGATTCGGCACCGCCAGCAGCTTTTTTGCACAGGCGAGATAAGAAACGTC‑GGGATACACCTTGCGGGGATAAAACGGATAACCTTCAAAGCCGGCCATCTGCGCACGCTTAATTTctg                                              >  2:1761794/1‑236 (MQ=255)

                                                                                                                                                                                                    ggCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGGAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGGC‑GGTATACACCCTGCGGGTATAAACCGGAAAACCCTCAAGGCCGTCCATCCGCGCACGCTtcacttcact                                             >  1:1076695/1‑239 (MQ=255)

                                                                                                                                                                                                           ccATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAg                                      >  1:286006/1‑239 (MQ=255)

                                                                                                                                                                                                                 ccgcgcacg‑cacTGGAACTAG‑TACTGACGCGGGTTG‑TGGTTCGCCCCCGCAGGTGGATAAATCGCCGCGAGCGTAGGGGCGTTGGGCGTCGCGAACTGGGGGGAGATTAGATTCGGCGCCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATGTCAGTATCCCAGTAc                                   <  2:2034262/233‑1 (MQ=255)

                                                                                                                                                                                                                    tGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATaca                                                                                                          >  1:1737677/1‑163 (MQ=255)

                                                                                                                                                                                                                    tGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGTGGGtata                                                                                             <  1:332302/176‑1 (MQ=255)

                                                                                                                                                                                                                    tGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGTGGGtata                                                                                             >  2:332302/1‑176 (MQ=255)

                                                                                                                                                                                                                    tGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTAcg                                  >  2:845563/1‑235 (MQ=255)

                                                                                                                                                                                                                    tGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGAATaaa                                                                                           >  1:867370/1‑178 (MQ=255)

                                                                                                                                                                                                                      cAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCtt                            >  2:2142476/1‑239 (MQ=255)

                                                                                                                                                                                                                      cAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATGAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACCCCTTGCGGGTATAAACAGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTCTCCCAGTAcgccactt                            >  2:1906916/1‑234 (MQ=255)

                                                                                                                                                                                                                         g‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     <  1:228149/148‑1 (MQ=255)

                                                                                                                                                                                                                         g‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGAC‑GGTATACACCTTGCGGGTATAAACCGGATAACATTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCa                                       >  1:2172877/1‑225 (MQ=255)

                                                                                                                                                                                                                            aCTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCcag                                                                                                                                                    <  1:1359982/115‑1 (MQ=255)

                                                                                                                                                                                                                            aCTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCcag                                                                                                                                                    >  2:1359982/1‑115 (MQ=255)

                                                                                                                                                                                                                             cTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCa                      >  1:171843/1‑239 (MQ=255)

                                                                                                                                                                                                                             cTGAAACTCG‑TCCTGCCCCGGGTAG‑TATTTCCGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCa                      <  2:1139178/239‑1 (MQ=255)

                                                                                                                                                                                                                               ggAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAg                     >  1:158338/1‑238 (MQ=255)

                                                                                                                                                                                                                                      g‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAatca             >  2:793969/1‑239 (MQ=255)

                                                                                                                                                                                                                                                  ggTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACg                                                                                                                     >  1:1969676/1‑125 (MQ=255)

                                                                                                                                                                                                                                                  ggTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATa                                                                                    >  2:1019382/1‑157 (MQ=255)

                                                                                                                                                                                                                                                  ggTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATa                                                                                    <  1:1019382/157‑1 (MQ=255)

                                                                                                                                                                                                                                                  ggTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAgacggcg   >  1:453735/1‑238 (MQ=255)

                                                                                                                                                                                                                                                   gtag‑taGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAgacggcga  <  1:793969/238‑1 (MQ=255)

TCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCAGCAGGCGACGCACCAGATACGCCAACAGCGTTTCATGTGT‑GCCAACCGGAGCATAAATACGACACGGAC‑GGTTAAGTTTGCCGTCGGCAACTTTCCC‑GGTGACCTGC‑TCATACAGTGGCTCGCCCATA‑CCATGCAGG‑CACTGGAACTCG‑TACTGACCCGGGTAG‑TAGTTCTGCCCCGCCAGTTGATAAATCGCCGCCAGCGTATGGGCGTTGTGCGTCGCGAACTGCGGGTAGATTAGATTCGGCACCGCCAGCAGCTTTTTCGCACAGGCGAGATAAGAAACGTC‑GGTATACACCTTGCGGGTATAAACCGGATAACCTTCAAGGCCGTCCATCTGCGCACGCTTAATTTCACTATCCCAGTACGCGCCTTTCACCAGGCGAATCATCAGACGGCGA  >  NC_007779/1077557‑1078030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`