BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	1,748,986	G→A	9.4%	E48K (GAA→AAA)	Y75_RS08785 →	FAD‑NAD(P)‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,748,986	0	G	A	9.4%	474.6 / 7.5	155	E48K (GAA→AAA)	Y75_RS08785	FAD‑NAD(P)‑binding protein
Reads supporting (aligned to +/- strand): ref base G (93/46); new base A (2/14); total (95/60)
Fisher's exact test for biased strand distribution p-value = 3.47e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTGGCTGTTTA  >  NC_007779/1748749‑1749224

 tttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaa                                                                                                                                                                                                                                               <  1:272974/239‑1 (MQ=255)

   tCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGAGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                                                                                                                                                                                                                                             <  2:467730/239‑1 (MQ=255)

     aTCCCTGTCTGAACTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAAc                                                                                                                                                                                                                                            >  2:1752092/1‑238 (MQ=255)

          tgtgtgAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGTTAAGTAACAATGAAAAAAATTGCTATTGAGGGTGCCGTGCCTACGGGCATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACATTGATGAGGAAAACTCaaaa                                                                                                                                                                                                                                      >  2:1982959/1‑239 (MQ=255)

          tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaa                                                                                                                                                                                                                                      >  1:1384344/1‑239 (MQ=255)

          tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaa                                                                                                                                                                                                                                      >  2:1823473/1‑239 (MQ=255)

           gTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaaa                                                                                                                                                                                                                                     <  2:2153636/239‑1 (MQ=255)

            tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAat                                                                                                                                                                                                                                    >  1:1077547/1‑239 (MQ=255)

            tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAat                                                                                                                                                                                                                                    >  2:186427/1‑239 (MQ=255)

                aaaTTTCTAAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatgatg                                                                                                                                                                                                                                <  2:2139856/239‑1 (MQ=255)

                 aaTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatgatg                                                                                                                                                                                                                                <  1:1644334/238‑1 (MQ=255)

                     tctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGc                                                                                                                                                                                                                           <  1:1115645/239‑1 (MQ=255)

                      ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCa                                                                                                                                                                                                                          >  2:743026/1‑239 (MQ=255)

                              cTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGGGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  <  2:347676/239‑1 (MQ=255)

                              cTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  <  1:95062/239‑1 (MQ=255)

                              cTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  <  1:1823473/239‑1 (MQ=255)

                                         aaCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat                                                                                                                                                                                                                       <  1:1763210/223‑1 (MQ=255)

                                              aaataaTCTGAAAACACTAAATTCAAAAGAAGGCGTACAGGAAAGTAACAATTAAAAAAATTTCTATTGTGGTTGCCGGGCCTACGGGGATCTACCCTTTATTCTCGCTTCTACAGAAACAAACTCCACTTTCTATTCCTATCTTCGAGAAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAACCTCAAAAATGATGCGGGCAAATATTGC‑CAGTATTGAAATAccg                                                                                                                                                                                                 <  2:1408644/234‑1 (MQ=255)

                                              aaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCAACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAAGGATGCTGGCAAATATTGC‑CAGTATTGAAATAccg                                                                                                                                                                                                 <  1:383072/239‑1 (MQ=255)

                                                   aTCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGAt                                                                                                                                                                                            <  2:1077547/239‑1 (MQ=255)

                                                   aTCTGATAACACTAAATGCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATACTCGCTTCAACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGAt                                                                                                                                                                                            >  2:754334/1‑239 (MQ=255)

                                                      tgttaaacttAAATTCAAATGAATGCGTCAGGGTAAGTAACAATTAAAAAATTTTTTTTGGTGGGTACCGCGCCTACGGGTATCTACCCCTTATTCTCGCTTCTACAGCAACAAACTCCCTTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCGGGTGTCGGGATGCCATACAGTGATGAGAAAAACTCAAAAATGATGCTGGAAAATATTGC‑CAGTATTGAAATACCGCCGATTTa                                                                                                                                                                                         <  2:2156224/230‑1 (MQ=255)

                                                        aaaaCACAAAATTCAAATGAATGCTTACAGGGAATAAACAATAAAAATATTTATTAGTGTGGGTGCCGGTCCACCGGATACCTACTCTTTTTTCCCGCTTCTACAGCAACAAACACCTCTTTTTTTTTCAATCTGCGAGCAGGCGGACGCGGCCGTTGGCGTTATGCCTTACAGTGATTAAGAAAACTAAAAAGTGTTGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTa                                                                                                                                                                                         <  2:1173920/235‑1 (MQ=255)

                                                                 aaTTCGAAAGAATGCGTACAGTTAAGTAACAATGAAAAATATTGCTATTGTGCGTGCCGTTCCTACGGGGATCTACGCCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATGTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGATGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  <  2:103053/204‑1 (MQ=255)

                                                                       aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGAATTGAAATACCGCCGATTTATTGTACGTATCTCGAAt                                                                                                                                                                        >  1:1414778/1‑239 (MQ=255)

                                                                           aaTGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGaa                                                                                                                                                                                                       >  1:103053/1‑204 (MQ=255)

                                                                             tGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  <  1:1715705/192‑1 (MQ=255)

                                                                             tGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                                                                                  >  2:1715705/1‑192 (MQ=255)

                                                                             tGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTAc                                                                                                                                                                  >  2:761679/1‑239 (MQ=255)

                                                                                gTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACCCCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGAAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACaaa                                                                                                                                                               <  1:1756283/239‑1 (MQ=255)

                                                                                     ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaa                                                                                                                                                          >  1:2113976/1‑239 (MQ=255)

                                                                                         agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaagaag                                                                                                                                                      >  1:412581/1‑239 (MQ=255)

                                                                                         agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaagaag                                                                                                                                                      >  2:843910/1‑239 (MQ=255)

                                                                                         agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTAC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaagaag                                                                                                                                                      >  2:654817/1‑239 (MQ=255)

                                                                                          ctaaAAATGAAAAAAATTGCTATTGGGGGTGCCGGTCCTACGGGTATATCCACCTTATTCACGCTTCTACAGCAACAATCTCCACTTGCTATTTCTACCTTGGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATTCAGTGATGAGAAAAACTCAAAGATGATGCTGGCAAATATTGC‑CAGGATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAagaaga                                                                                                                                                     <  1:2010482/238‑1 (MQ=255)

                                                                                           taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGAc                                                                                                                                                    >  1:1086615/1‑239 (MQ=255)

                                                                                            aaCAATGAAAAAATTTGCTTTTGGGGGTGCCGGGCCTCCGGGGATCTACACTTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCGGGTGTCGGGATGCCATACAGTAATGAGAAAAACTCAAAAATGATGCTGGAAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAagaaga                                                                                                                                                     <  2:1384344/237‑1 (MQ=255)

                                                                                            aaCAAGGAAAAAAATTGCTAGTGTGGGGGCCGGACCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAACCTCCACTTTCTATTTCAATCGTCGAGCAGGCTGACGAGGCGGGTGTCGGGATGCCATGCAGTGACGAGGAAAACTCAAAATTGATGCTGGCAAATATTGC‑CGGTATGGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAagaaga                                                                                                                                                     <  1:2123522/237‑1 (MQ=255)

                                                                                                aTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc‑c                                                                                                                                                                                                                >  2:1136057/1‑174 (MQ=255)

                                                                                                aTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc‑c                                                                                                                                                                                                                <  1:1136057/174‑1 (MQ=255)

                                                                                                     aaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGc                                                                                                                                                 <  1:1662840/232‑1 (MQ=255)

                                                                                                     aaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGc                                                                                                                                                 >  2:1662840/1‑232 (MQ=255)

                                                                                                     aaaaaTGCCTATTTTGGTGGCCGGCCCTACGGGGACCTACACCTAATTCTCACTTCAACAGCAACAAACTCCACTTTCTATTTCTATCTTCGACAAGGCTGACGAGGCCGGTGTCGGGTTGCCATACAGTGATGAGAAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGAATTAAAATACCGCCGATTTATTGAACTAATCTC‑AATGGACAAAAAAACAACAAAACAACCaatccca                                                                                                                                         >  2:214390/1‑233 (MQ=255)

                                                                                                           tGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGtt                                                                                                                                    >  1:1511286/1‑239 (MQ=255)

                                                                                                                  gtgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGt                                                                                                                             >  1:481113/1‑239 (MQ=255)

                                                                                                                     ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAATACAGCCATCTCCAGCGTTATGGCGTTaa                                                                                                                          >  2:550565/1‑239 (MQ=255)

                                                                                                                     ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTaa                                                                                                                          >  1:456732/1‑239 (MQ=255)

                                                                                                                       tGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTATATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGCCGGGATGCCATCCAGTGATGAGGAAAACTCAAAAATGATGATGGCAAATATTGC‑CAGTATTGAAACACCGCCGATTTATTGTCCGTCTCTGGAATGCCTACAAAAGAAAGAAGACAGCAATCTCCAGCCGTGTGGCGTTaaaa                                                                                                                        >  2:298283/1‑239 (MQ=255)

                                                                                                                          cGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAg                                                                                                                     <  1:827434/239‑1 (MQ=255)

                                                                                                                            ggCCTACGGGGATCTACGCCTTATTCTCGCTTCTACAGCAACAAACTCATCTTATTATTTCTATCTTAGAGCAGGCAGACGACGCCGGAGTTGGGATGCCATACAGTGAGGAGGTAAAATCAAAAGTGATGATGGGAAATATTGC‑CAGAATGGAAATACAGCCGATTTATGGTACGAATCGCGAATGGCTACAAAATCACGAAGACAGCCAGCTCAAGCTTAATGGCGTTAaaaaaaaa                                                                                                                   >  2:1677379/1‑239 (MQ=255)

                                                                                                                                     ggATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCa                                                                                                           <  1:761679/238‑1 (MQ=255)

                                                                                                                                       aTCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGa                                                                                                        >  1:625934/1‑239 (MQ=255)

                                                                                                                                       aTCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGa                                                                                                        >  2:1408831/1‑239 (MQ=255)

                                                                                                                                                 tATTCTCGCTTCTACAGCAACAAACTCCACTTTCTCTTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGCGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGCAATACCGCCGATTTATTGTACGTATCTCGAATGGCGACAAAAGCAAGAAGACCGCCATCACCAGCGTTATGGCGTTAAACACGAAACCTTGCACGATctgccattg                                                                                              >  2:2089781/1‑231 (MQ=255)

                                                                                                                                                 tATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGttt                                                                                              >  2:279441/1‑239 (MQ=255)

                                                                                                                                                  aTTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGtttt                                                                                             >  1:1086665/1‑239 (MQ=255)

                                                                                                                                                   ttCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGttttt                                                                                            >  1:2226800/1‑239 (MQ=255)

                                                                                                                                                        gCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACcgc                                                                                       >  1:387873/1‑239 (MQ=255)

                                                                                                                                                         cggcTACAGCAAAAAACTCCAATTTTAATTTCTATCTACGAGCCGGGTGACGAGGCCGGTGTCGGGATGCCATACAGTGAGAAGGAAACCTAAAAAATGATGCTGGAAAATATTGC‑CAGTATGGAAAAACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACcgc                                                                                       <  2:1303169/235‑1 (MQ=255)

                                                                                                                                                                   aacaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCGGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTAAAATGCCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTctgc                                                                              <  1:186427/237‑1 (MQ=255)

                                                                                                                                                                    acaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTgg                                                                           >  1:1120083/1‑239 (MQ=255)

                                                                                                                                                                    acaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTgg                                                                           >  1:747938/1‑239 (MQ=255)

                                                                                                                                                                    acaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTgg                                                                           >  1:1810567/1‑239 (MQ=255)

                                                                                                                                                                       aaCTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTctgct                                                                             >  1:1303169/1‑234 (MQ=255)

                                                                                                                                                                             aCTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGACAGTTTTTACCGCGAATTCTGCTGGGCGAATCtt                                                                  >  2:1677206/1‑239 (MQ=255)

                                                                                                                                                                              cTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATAtt                                                                  >  2:869035/1‑238 (MQ=255)

                                                                                                                                                                                 tctatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTcc                                                               >  2:1114182/1‑238 (MQ=255)

                                                                                                                                                                                 tctatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTcc                                                               >  1:1440600/1‑238 (MQ=255)

                                                                                                                                                                                 tctatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGAGGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAACTATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGAAAGAACATAGCCATCTCCAGCGTTACGGCGTTAAAAACGAAACCTTGCACGATAGACAGTTTTTACAGCGAATTCTGTCGGGCGAAGAttcacg                                                              >  2:741861/1‑235 (MQ=255)

                                                                                                                                                                                           tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAGGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGACTTCTGCTGGGCGAATATTTCCGCGATCACttt                                                    >  1:482861/1‑239 (MQ=255)

                                                                                                                                                                                           tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAAttt                                                    >  2:2034497/1‑239 (MQ=255)

                                                                                                                                                                                           tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAAttt                                                    >  1:1229190/1‑239 (MQ=255)

                                                                                                                                                                                           tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAAttt                                                    >  1:1712770/1‑239 (MQ=255)

                                                                                                                                                                                           tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTAACGCGAATTCTGCTGGGCGAATATTTCCGCGATCAAttt                                                    >  2:598273/1‑239 (MQ=255)

                                                                                                                                                                                           tCTTCCAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACg                                                                                                         <  1:1184302/186‑1 (MQ=255)

                                                                                                                                                                                             ttCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAAttttt                                                  >  1:909090/1‑239 (MQ=255)

                                                                                                                                                                                               cGAGCATGCTGACGAGGCCGGTGTCGGGATGCCATACTATGATGAGGAAACCCCAAAAATGATGCTGGCAAATATTGC‑CGGTATGGAAAAACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGTGCGAATATTTCCGCGATCAATTTTTAc                                                <  1:1982959/239‑1 (MQ=255)

                                                                                                                                                                                                gAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACg                                               <  1:843910/239‑1 (MQ=255)

                                                                                                                                                                                                     ggCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACtag                                          >  1:40473/1‑239 (MQ=255)

                                                                                                                                                                                                                ccGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGc                                >  2:1767244/1‑238 (MQ=255)

                                                                                                                                                                                                                   gtgtCGGGATGCCATACGGTAAGGGGAAAAACCCAAAAATTATCCTGGAAAGTATTGC‑AATTAATAAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACg                             <  2:1983855/238‑1 (MQ=255)

                                                                                                                                                                                                                    tgtCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACACCCATCTCCAGCGTTATCGCGTTAAACAAGCAACCTTGAACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAAACCAAGCATGaa                           >  2:43931/1‑238 (MQ=255)

                                                                                                                                                                                                                                  aCAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCa             <  1:347255/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAg                                                                                                                                                  >  1:746112/1‑104 (MQ=255)

                                                                                                                                                                                                                                       gatgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTgg         <  1:604087/238‑1 (MQ=255)

                                                                                                                                                                                                                                       gatgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTg          >  1:990196/1‑237 (MQ=255)

                                                                                                                                                                                                                                       gatgaGGAAAACTCAAAAATGATGCTGACAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGt           <  1:214390/236‑1 (MQ=255)

                                                                                                                                                                                                                                       gatgaGAAAAACTCAAAAATGTGGCTGACAAATATAGC‑AATTAAAGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCTCGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTgg         <  2:1712770/238‑1 (MQ=255)

                                                                                                                                                                                                                                       attgaGGAAACCTAAAAAATGGTG‑TGGGAAAGAATGCGCAGTATGGAAATGCCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTgg         <  2:625934/236‑1 (MQ=255)

                                                                                                                                                                                                                                        gtggggAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTTAAATCCCCCCGTGTTATTTTTCCTCTCCCGAATGGCCACAAAAGCAAGAAAACAACCATCTCCACCGCTATGGCGCTAACTAATAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGTCGAATATTTCAGCGATCAATTTTTACGACTCGTAGACCACGCATGCTAGCAAAAATTGGCAGTg          >  2:1630910/5‑236 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAACAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTctg                                                                               >  1:1691574/1‑167 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCGGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGAAGGGGcg       >  2:2105301/1‑238 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACCGCAAAAATTTGCAGTg          >  2:555024/1‑236 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTg          >  1:895509/1‑236 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTg          >  2:1065898/1‑236 (MQ=255)

                                                                                                                                                                                                                                        atgaGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTg          >  1:1967143/1‑236 (MQ=255)

                                                                                                                                                                                                                                        atgaGAAAACCTCAAAAAGGATGCTGGCAAATATGAC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTGGCt       <  1:2004795/239‑1 (MQ=255)

                                                                                                                                                                                                                                         tgaGAAAAACTCAAAAATGATACTGACAAATATTGC‑CATTATAGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTGGc        <  2:969313/237‑1 (MQ=255)

                                                                                                                                                                                                                                           aGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTGGCTGtt    >  2:1502306/1‑239 (MQ=255)

                                                                                                                                                                                                                                             gAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGc                                                                         >  2:826713/1‑168 (MQ=255)

TTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGC‑CAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTCCGCGATCAATTTTTACGACTAGTAGACCAGGCACGACAGCAAAAATTTGCAGTGGCTGTTTA  >  NC_007779/1748749‑1749224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`