Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,153,722	C→T	23.3%	P134L (CCT→CTT)	Y75_RS10870 →	YegJ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,153,722	0	C	T	23.3%	106.4 / 9.0	46	P134L (CCT→CTT)	Y75_RS10870	YegJ family protein
Reads supporting (aligned to +/- strand):  ref base C (3/32);  new base T (3/8);  total (6/40)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTT  >  NC_007779/2153486‑2153957                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             aaaaGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCtt                                                                                                                                                                                                                                            <  1:1077761/239‑1 (MQ=255)      aTGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGGTT‑TTACAATTGTTCTtttttt                                                                                                                                                                                                                                        >  1:982301/1‑239 (MQ=255)        gCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGt                                                                                                                                                                                                                                     <  2:977511/239‑1 (MQ=255)        gCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGGT‑ATACCATTTATCTttttttttt                                                                                                                                                                                                                                     >  1:737408/1‑236 (MQ=255)           gATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCAc                                                                                                                                                                                                                                  <  1:257302/239‑1 (MQ=255)           gATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCAc                                                                                                                                                                                                                                  <  1:181991/239‑1 (MQ=255)                                 cGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCcgac                                                                                                                                                                                                            <  2:1211343/239‑1 (MQ=255)                                      aCCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCt                                                                                                                                                                                                       <  1:1486578/239‑1 (MQ=255)                                              cGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCaa                                                                                                                                                                                               <  1:1300492/239‑1 (MQ=255)                                                     aCCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTa                                                                                                                                                                                          <  1:86481/237‑1 (MQ=255)                                                        gAGCATTTCTGGCTTACGGATGTCACTTATTCTAACTGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTAATCCTTTATTGTTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCg                                                                                                                                                                                     <  2:1181390/239‑1 (MQ=255)                                                         aGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑TAATCATTTTTCTTTTTTTTGTGACCCATTCCAAAAAAAAAAACGGCGAGGCAAGAGCaaaaaaatatgg                                                                                                                                                                                     >  1:1208123/1‑230 (MQ=255)                                                             tttCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAt                                                                                                                                                                                <  1:521755/239‑1 (MQ=255)                                                             tttCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAt                                                                                                                                                                                <  2:101123/239‑1 (MQ=255)                                                             tttCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAt                                                                                                                                                                                <  1:940837/239‑1 (MQ=255)                                                             tttCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAt                                                                                                                                                                                <  1:1251828/239‑1 (MQ=255)                                                                cTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGTCCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAg                                                                                                                                                                             <  1:270516/239‑1 (MQ=255)                                                                cTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAg                                                                                                                                                                             <  2:1331615/239‑1 (MQ=255)                                                                cTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAg                                                                                                                                                                             <  1:853485/239‑1 (MQ=255)                                                                cTGGCTTACGGAATTCACTTATTCTAACGGCAGGTTTATTGGGGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAg                                                                                                                                                                             <  2:710469/239‑1 (MQ=255)                                                                    cTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCg                                                                                                                                                                          <  2:1366524/238‑1 (MQ=255)                                                                     ttACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGt                                                                                                                                                                         <  1:1691419/238‑1 (MQ=255)                                                                       aCGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTtat                                                                                                                                                                      <  1:756490/239‑1 (MQ=255)                                                                            tGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattta                                                                                                                                                                 <  1:664439/239‑1 (MQ=255)                                                                               cACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtatttatt                                                                                                                                                               <  1:1794452/238‑1 (MQ=255)                                                                               cACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTg                                                                                                                                                              <  2:271895/239‑1 (MQ=255)                                                                                         aaCGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTgg                                                                                                                                                    <  1:629702/239‑1 (MQ=255)                                                                                               aTGTTTATTGGGGTAATCAACAATGGCACTGGTAATATTACAAACGTAGAATATGGCCAGGAGTGGAGAATCAAGAAAGGAGATATTTCAGTCGGGATGTATCCGCGCCGGGATAAAATTTAGGTG‑GGTT‑GTTCCATTGATCCTGCATTGGTCGCCAATCCAAAAGAATAAGCCGACGTTCTAAGAACTCAATTAGTTCGCTATTCAGCCGTTATATTTCTTGTAGAAAATGGCGTTTg                                                                                                                                              <  2:1805281/239‑1 (MQ=255)                                                                                               aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                                                                                                               <  2:648949/238‑1 (MQ=255)                                                                                                 gTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGaa                                                                                                                                            <  1:993444/239‑1 (MQ=255)                                                                                                                         cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCg                                                                                                                                                                                     <  1:34524/174‑1 (MQ=255)                                                                                                                          ccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCAc                                                                                                                                                                                                                                  <  1:1948857/128‑1 (MQ=255)                                                                                                                               tATTTTTAAAAACGTAGAATATGGCAGGGAGGGAAAAAAAAAAAAAGAAGATATTTAAGACTGGATGTATACGCGGGGGAAAAAAATTTAGGGG‑GGTT‑ATACCATTGTTCTTTTATGGGTCACCTATCAAAAAAAAGAAGCCGACGAGAAAAGAGAAAAATTAGTTCGCTAACAAGCCGTTATATTTTTTGTTAAACCGGGGGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAactc                                                                                                              <  1:658465/238‑1 (MQ=255)                                                                                                                                  tGTTACACACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGTATGTATACGCGCGGGGATAAAGTTGACGGG‑GTTT‑ATACCATTGATCCTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGGCGATCTAAGAGAAAATTTATTTCTCTAATCATCCGTTATTTTTATTGTTTAAACGGGCGTTTGAA‑TTGTTTTCTTCAACCGCCATGAGAAactcactc                                                                                                          <  1:5023/239‑1 (MQ=255)                                                                                                                                    ttACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCaa                                                                                                                                                                                               <  1:634200/153‑1 (MQ=255)                                                                                                                                                                  aaaaaaaaaaagaaGATTTTCCAGACTGGATGTATACGGGCGGGGATAAAATTT‑CGGG‑GGTTAATACCATTGATCTTTTATTGGCCCCCTACCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGTTAACCAGCCGTTATTTTTTTTGTTTAAACTGGCGGTTAAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                          <  1:1317861/231‑1 (MQ=255)                                                                                                                                                                     taaagaaaaaAGATATTTCAGACTGGATTTATCCGCGGGGGAAAAAAATTTACGGC‑GGTT‑ATCCCATTGATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGTTAACCAGCCGTTATATTTATTGTTAAACCGGGGGTTTGAA‑TTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                       <  1:1212624/237‑1 (MQ=255)                                                                                                                                                                        aaaaagaaGATATTCCAGACTGGATGTATACGCGCGGGGAAAAAATTTACGGG‑GGTT‑ATACCATTGATCTTTTATTGGTCACCTATCAAAAAAAAGAAGCCGACGATCTAAGAGCAAAATTAGTTCGCTAATCAGCTGTTATTTTTATTGTTTAAGCGGGGGGTTAAT‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                    <  1:132532/237‑1 (MQ=255)                                                                                                                                                                                              gATGTATCCGGGGGGGAAAAAATTTTAGGGG‑GTTT‑ATCATTTTGTTCTTTTATGGGTCCCTTACCAAAAAAAAAAACCCGAGGAGCAAAGAAAAAATTTATTTCGCAAACCAGCCTTTATTTTTTTTGTTAAAACGGGGGTTTAAA‑TTGTTTTCTTAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                               <  1:1404532/238‑1 (MQ=255)                                                                                                                                                                                              gATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAg                                                                                                                                                                             <  1:181237/113‑1 (MQ=255)                                                                                                                                                                                                                      ttccggc‑gtTT‑ATACATTTGTTCTTTTTTTGGTCACTTACAAAAAAGAAGACGCCGACGAGCAAAGAGAAAATTTTTTTCGCTAACCAGCTGTTATATTTTTTTTTAAACCTGGCGTTTGAT‑TTTTTGTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                      <  1:623048/236‑1 (MQ=255)                                                                                                                                                                                                                      ttccggc‑ggTT‑ATACCATTAATCTTTTTTTGTTCCCCTACCAAAAAGAAAAACCCGAGGAGTAAAGAAAAAAATTATTCCGTAAACCAGCTGTTATTTTTATTTTTTAACCGGGGGGTTAAT‑TTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                      <  1:33036/236‑1 (MQ=255)                                                                                                                                                                                                                                       cATTGTTCTTTTATTGTTCCCCTACCAAAAAAAAAAGGCCGACGAGCAAAGAAAAAAATGATTTCGCTAACCAGCCTTTATATTT‑TTTTTTAAACCGGGGGTTTAATTTGTTTTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg       <  1:990623/239‑1 (MQ=255)                                                                                                                                                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAAACGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGCACGCCCGGTTACCGCCGGGCTGGACAAAAACCGCTTTAACAATTCAGGGAATAAATccaagtg    >  1:1594775/1‑233 (MQ=255)                                                                                                                                                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTTTTCTACCGCCCTGAGAAAATCACTCTGCCCAAAGCGCCAGTGACAAAGATTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCCGGAAAAAAAAAACTTTAAAAAATAAGGTTATAAATAGAgtttt   >  1:1108360/1‑237 (MQ=255)                                                                                                                                                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt   >  1:1502539/1‑239 (MQ=255)                                                                                                                                                                                                                                              ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAACCGCCATGAGAAAATCAACCTGCCCACAGACCCAGTGCCAAAGTTTTATTATTATCCCTTCCCTCGTTTACCCCCGCGGTGGCCCAAAACCGCTTTAACAACTCTGGGACTAAATAGAgttgtt  >  1:1929764/1‑237 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             AAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGC‑GGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTT  >  NC_007779/2153486‑2153957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑