Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,153,826	G→T	28.7%	intergenic (+43/+22)	Y75_RS10870 → / ← pphC	YegJ family protein/protein‑serine/threonine phosphatase PphC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,153,826	0	G	T	28.7%	43.1 / 5.0	44	intergenic (+43/+22)	Y75_RS10870/pphC	YegJ family protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base G (22/8);  new base T (7/6);  total (30/14)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATC  >  NC_007779/2153612‑2154054                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       tATTTTTAAAAACGTAGAATATGGCAGGGAGGGAAAAAAAAAAAAAGAAGATATTTAAGACTGGATGTATACGCGGGGGAAAAAAATTTAGGGGGGTT‑ATACCATTGTTCTTTTATGGGTCACCTATCAAAAAAAAGAAGCCGACGAGAAAAGAGAAAAATTAGTTCGCTAACAAGCCGTTATATTTTTTGTTAAACCGGGGGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAactc                                                                                                                                                                                                                <  1:658465/238‑1 (MQ=255)    tGTTACACACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGTATGTATACGCGCGGGGATAAAGTTGACGGGGTTT‑ATACCATTGATCCTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGGCGATCTAAGAGAAAATTTATTTCTCTAATCATCCGTTATTTTTATTGTTTAAACGGGCGTTTG‑AA‑TTGTTTTCTTCAA‑CCGCCATGAGAAactcactc                                                                                                                                                                                                            <  1:5023/239‑1 (MQ=255)                                    aaaaaaaaaaagaaGATTTTCCAGACTGGATGTATACGGGCGGGGATAAAATTT‑CGGGGGTTAATACCATTGATCTTTTATTGGCCCCCTACCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGTTAACCAGCCGTTATTTTTTTTGTTTAAACTGGCGGTTA‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                                                                                                            <  1:1317861/231‑1 (MQ=255)                                       taaagaaaaaAGATATTTCAGACTGGATTTATCCGCGGGGGAAAAAAATTTACGGCGGTT‑ATCCCATTGATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGTTAACCAGCCGTTATATTTATTGTTAAACCGGGGGTTTG‑AA‑TTGTTGTCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                                                                                                                         <  1:1212624/237‑1 (MQ=255)                                          aaaaagaaGATATTCCAGACTGGATGTATACGCGCGGGGAAAAAATTTACGGGGGTT‑ATACCATTGATCTTTTATTGGTCACCTATCAAAAAAAAGAAGCCGACGATCTAAGAGCAAAATTAGTTCGCTAATCAGCTGTTATTTTTATTGTTTAAGCGGGGGGTTA‑AT‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                      <  1:132532/237‑1 (MQ=255)                                                                gATGTATCCGGGGGGGAAAAAATTTTAGGGGGTTT‑ATCATTTTGTTCTTTTATGGGTCCCTTACCAAAAAAAAAAACCCGAGGAGCAAAGAAAAAATTTATTTCGCAAACCAGCCTTTATTTTTTTTGTTAAAACGGGGGTTTA‑AA‑TTGTTTTCTTAAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGc                                                                                                                                                 <  1:1404532/238‑1 (MQ=255)                                                                                        ttccggcgtTT‑ATACATTTGTTCTTTTTTTGGTCACTTACAAAAAAGAAGACGCCGACGAGCAAAGAGAAAATTTTTTTCGCTAACCAGCTGTTATATTTTTTTTTAAACCTGGCGTTTG‑AT‑TTTTTGTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                        <  1:623048/236‑1 (MQ=255)                                                                                        ttccggcggTT‑ATACCATTAATCTTTTTTTGTTCCCCTACCAAAAAGAAAAACCCGAGGAGTAAAGAAAAAAATTATTCCGTAAACCAGCTGTTATTTTTATTTTTTAACCGGGGGGTTA‑AT‑TTTTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                        <  1:33036/236‑1 (MQ=255)                                                                                                        cATTGTTCTTTTATTGTTCCCCTACCAAAAAAAAAAGGCCGACGAGCAAAGAAAAAAATGATTTCGCTAACCAGCCTTTATATTT‑TTTTTTAAACCGGGGGTTT‑AATTTGTTTTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                         <  1:990623/239‑1 (MQ=255)                                                                                                           tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑ACGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGCACGCCCGGTTACCGCCGGGCTGGACAAAAACCGCTTTAACAATTCAGGGAATAAATccaagtg                                                                                                      >  1:1594775/1‑233 (MQ=255)                                                                                                            gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTTTTCTA‑CCGCCCTGAGAAAATCACTCTGCCCAAAGCGCCAGTGACAAAGATTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCCGGAAAAAAAAAACTTTAAAAAATAAGGTTATAAATAGAgtttt                                                                                                     >  1:1108360/1‑237 (MQ=255)                                                                                                            gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt                                                                                                     >  1:1502539/1‑239 (MQ=255)                                                                                                               ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTTTCTTCAA‑CCGCCATGAGAAAATCAACCTGCCCACAGACCCAGTGCCAAAGTTTTATTATTATCCCTTCCCTCGTTTACCCCCGCGGTGGCCCAAAACCGCTTTAACAACTCTGGGACTAAATAGAgttgtt                                                                                                    >  1:1929764/1‑237 (MQ=255)                                                                                                                  ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTT‑AA‑ATGTTTTTTTCAA‑CCGCCCTGAGAAACTCACCCTGCCCACCGCGCCAGGGCCAACGATTTATCCACCTCCATCCGCCCCTTTTCCCCCGGGCGGGGCAAAAAATCCTTTTACAAATCCGGTAATAAATCAAGGTTTTTCTg                                                                                                >  2:390681/1‑238 (MQ=255)                                                                                                                   tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTTTTCCA‑ACGCCATGAGAAAATCAATCTGCCCACAGCGCCCGTGTCAACGTTTTTTCATTATCCCTAACCCCGTTTTCCCCCCGGCTGGACAAAAAATGCCTTTACAAATCAGTGAAAAAAACAAGTTTGTCCTGc                                                                                               >  2:990623/1‑238 (MQ=255)                                                                                                                        gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                              >  1:962724/1‑154 (MQ=255)                                                                                                                          cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGCTG‑AA‑TTTTTTTCTTCAA‑CCCCCATGTGCAACCCACTTCTCCCACAGCGCCCGTTGCCACGTCTTATAATCATTCGTACCCTCCGTTACCGCCCGGCTGGACAAAAACCGCTTTTACCATTTTAGTCATAAAACGGATTGTTCCTGCGTttctg                                                                                        >  2:1413372/1‑238 (MQ=255)                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTTTTTTCTTCAA‑ACGCCCTGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGGTTTATAATCATCCGTACGCTCGTTTAACCCCGGGCTGGACAAAAAAAGCTTTAACAATTCAGGTAATAAAACAAGTTGTTCCTTAGTTTCTgcc                                                                                      >  1:216069/1‑239 (MQ=255)                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTTTCTTCAA‑CCGCCCTGAGAAACACAATCTCCCCACAACGCCAGCGTCAACCTTTTATCATCATCCGCACGCTCGTTTACCGCCCGGCTGGACACAAACTACTTTTACAACTCAGGTGAATAATCCAATTGTTCCTGCGTtgctgc                                                                                       >  2:1192958/1‑238 (MQ=255)                                                                                                                           aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCGGGACAAAAAATGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTgcc                                                                                      >  1:95733/1‑239 (MQ=255)                                                                                                                                tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg                                                                                 >  1:957373/1‑239 (MQ=255)                                                                                                                                tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg                                                                                 >  1:668635/1‑239 (MQ=255)                                                                                                                                          gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTT‑TA‑TTTTTTTTTTTAA‑ACCCCATGAGAAACTCACTCTCCCCACAACGCCACTGCCCACCATTTTTTATCATCCCTACCCCCGTTTTCCGCCCGGCTGGGCAAAAAATTCTTTTAACATTCATGGAATAAATAGAGTTGTTCCTTCGTTTCTGCCCCCCG‑TCCACTGaaa                                                                      >  2:591438/1‑239 (MQ=255)                                                                                                                                          gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑ACGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCCACGCTTTATCATCATCCTGACGCCCTGTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGGAAAAAATCGAGGTGTTCCTGCTGTGCTGCCGCCAG‑TCCATTCaaa                                                                      >  2:1653384/1‑239 (MQ=255)                                                                                                                                              ccgacggtatAAGAAAAAATTTAGTTCTCTAACCGGCCTTTATATTTTTTGTTAAATCGGGGGTTTA‑AT‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACg                                                                  <  1:1071880/230‑1 (MQ=255)                                                                                                                                                              aaaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAA‑TTTTTCGTTCCTT‑CCCCCAT‑AGAAAATTACCCCTCCCCCCGCGCCAGTGCCCCCCTTTTATTATCATTCGTAACCCCCTTTACCACCGGGGTGGACAAAAAATACTTTTAAAATTTTAGCAATAAAAAGAAGTGTTTCTGCTGTCCTGCCCCCCGATCCCTGAAAAAAAGGGGT‑AAGAAAAGca                                                   >  2:920683/1‑238 (MQ=255)                                                                                                                                                                aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTT‑AA‑TTGTTGTCTTCAA‑ACGCCATGAAAAACTCACCCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCCTCGGTGCTGCCGCCAG‑TCCATTGAAAAACGGGGGaaaaaaaagaaaat                                                >  1:1299609/1‑232 (MQ=255)                                                                                                                                                                aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTTTTCCTGCGTTTCTGCCGCCAG‑TCCCTTGAAAAACGGGGTAAAGAAAAGCACAt                                                >  1:1543015/1‑239 (MQ=255)                                                                                                                                                                          cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑TA‑TTTTTTTCTTCCA‑CCGCCCTGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCCTACCCTCCTTTACCGCCCGGGTGGGCAAAAACTGCTTTTACCATTTCGGTAATAAATCGAGTTGTTCCTGCCTTTGTTCCCCCCG‑TACCTTTCAAAAAGGGGGAAAAAAAGGGACCTGAGGAGAAtt                                      >  2:238461/1‑239 (MQ=255)                                                                                                                                                                          cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCCCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAAtt                                      >  1:1236415/1‑239 (MQ=255)                                                                                                                                                                             aTCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCCA‑CCCCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGGTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGCCAAAAAATGCTTTAACAATTCAGGTAATAAATCCAGTTGTTCCTGCGTTTGTGCCGCCCG‑TCCCTTTAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTAt                                    >  1:980780/1‑238 (MQ=255)                                                                                                                                                                             aTCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCCTCCGTa                                                                                                                                                                    >  1:386583/1‑111 (MQ=255)                                                                                                                                                                              acaaccGTTATTTTTTTTGTTTAAACGGGGGGTTT‑GA‑TTTTTTTTTTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                                   <  1:1352603/235‑1 (MQ=255)                                                                                                                                                                                  cttttATTTTTTTTTTTAAAACGGGGGTTTA‑AT‑TTTTTGTTTCCAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                              <  1:712364/236‑1 (MQ=255)                                                                                                                                                                                     ttATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCCGCGTTGCTGCCGCCCG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCatat                           >  1:1457106/1‑239 (MQ=255)                                                                                                                                                                                          tttattGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCCA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTCCGTTGCTGCCGCCCG‑TCCCTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAc                      >  1:10739/1‑239 (MQ=255)                                                                                                                                                                                            tattGTTTAAACTGGCGGTTG‑AA‑TTGTTGTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACCGCTTTTACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAAtt                                      >  1:456801/1‑221 (MQ=255)                                                                                                                                                                                               tGTTTAAACTGGCGGTTG‑CA‑TTGTTGTCTTCTA‑CCGCCCTGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGCGTTGTTCCTCCGTTGCTGCCGCCAG‑TCCCATGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACCCCAg                 >  1:1027192/1‑239 (MQ=255)                                                                                                                                                                                                tttaccccTGGTGGTTG‑TA‑TTTTTTTCTTACC‑CCGCCATGAAAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGTACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCTAGt                <  1:1026780/232‑1 (MQ=255)                                                                                                                                                                                                  ttAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGCGTTTCTGCCGCCAG‑TCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTc               >  1:1487949/1‑238 (MQ=255)                                                                                                                                                                                                   tAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTc               >  1:1488136/1‑237 (MQ=255)                                                                                                                                                                                                        tGGGGGTTG‑AT‑TTGTGTGCTACAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa         <  1:774480/238‑1 (MQ=255)                                                                                                                                                                                                           cGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCCTCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAAACGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATaac     >  1:107457/1‑237 (MQ=255)                                                                                                                                                                                                               tG‑AA‑TTGTTGTCTTCAA‑CCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATc  >  1:474496/1‑238 (MQ=255)                                                                                                                                                                                                                          gtCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATaaa                                                                                                              >  1:1377796/1‑122 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       TATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTT‑ATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG‑TCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATC  >  NC_007779/2153612‑2154054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑