Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,999,815	T→C	13.5%	L285P (CTC→CCC)	xdhA →	xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,999,815	0	T	C	13.5%	184.5 / 2.6	66	L285P (CTC→CCC)	xdhA	xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base T (52/4);  new base C (2/8);  total (54/12)
Fisher's exact test for biased strand distribution p-value = 3.41e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATG  >  NC_007779/2999581‑3000047                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         aggaTGACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGGATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCTCCGCa                                                                                                                                                                                                                                      >  2:1821489/1‑239 (MQ=255) aggaTGACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGc                                                                                                                                                                                                                                       <  2:272212/238‑1 (MQ=255) aggaTGACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGc                                                                                                                                                                                                                                       <  1:31855/238‑1 (MQ=255) aggaTGACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCa                                                                                                                                                                                                                                      >  1:1165292/1‑239 (MQ=255)      gACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGGAGAGTGTTTCCTCGCAACCCg                                                                                                                                                                                                                                 >  1:123671/1‑239 (MQ=255)      gACTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGGGTTTCCTCGCAACCCg                                                                                                                                                                                                                                 >  1:225096/1‑239 (MQ=255)       aCTCGCGAATTACCATCGTTTCCAGCGCCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGt                                                                                                                                                                                                                                >  1:1643068/1‑239 (MQ=255)                               gcgcccAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAAAAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTTACCAGCAAGCTT‑GGCGGCATTCCGG‑GGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGGGTTTCCTCGCCACCCGTCACCGCCCCGCTTTTACCATTGAc                                                                                                                                                                                                        >  2:987785/4‑239 (MQ=255)                                  cccAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCCCGCTTTTACCATTGACgg                                                                                                                                                                                                      >  1:1662200/1‑238 (MQ=255)                                                 ttGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGGGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGaa                                                                                                                                                                                       >  1:346026/1‑238 (MQ=255)                                                  tGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGGAGAGCCAATGGCGGCATTCCTGACCAGCCAGCTT‑TGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGGGTTTCCTCGCAACCCGTACCCGCCCCGCTTTTACCATTGAAGGGCAAATGGGCgggcac                                                                                                                                                                                      >  2:777987/1‑233 (MQ=255)                                                                          gcTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtata                                                                                                                                                             >  1:1308882/1‑239 (MQ=255)                                                                              gATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGCTTTGGTAATAAACCGGATGTACTTGAAGAGCCAATGGCGGCCTTCCCTGCCCGCCAGCTTGGGTGGCA‑TCCGG‑GGAAAGGTT‑CCC‑CTC‑GCCGTGAAGAGGGTTTCCCCGCAACCCGTACCCGGCCCGCTTTTACCCTTTCCGGGCCAATTGGCGTGGCCCGCGCTGGCACCCTGAAAGGTtaaatgct                                                                                                                                                         >  2:1438341/1‑233 (MQ=255)                                                                                  ttCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtata                                                                                                                                                             >  1:1067857/1‑231 (MQ=255)                                                                                    cccTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGt                                                                                                                                                   >  1:1575666/1‑239 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCCTTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGGTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGt                                                                                                                                                   >  1:1543585/1‑235 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTAcc                                                                                                                                                                                                              >  1:766712/1‑176 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATgg                                                                                                                                                                                              >  1:1156645/1‑192 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGt                                                                                                                                                   >  1:253356/1‑235 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGt                                                                                                                                                   >  1:1767581/1‑235 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGt                                                                                                                                                   >  1:459270/1‑235 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGAAAGCTT‑GGCGGCATTCCGG‑TGAAAGGTT‑CCC‑TTA‑GCCGCTAAGAGTGGTTCGTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGGGTTAACCGCGACGGAACCTTTAACGGGTATAGTCTGGATGTTCTg                                                                                                                                               >  2:608514/1‑239 (MQ=255)                                                                                        ggTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAAAGGGGGCATTTCGGACTAGCAAGGTT‑GGGGGCATTCCGG‑GGAAAGGTT‑CCC‑TTA‑GCCGTGAAGAGTGGTTTCTCGCAACCCGTACCCGCCCCGCTTTTACCAATGATAGGCAAATGGGAGGGAACCGCGACGGAACATTGGAAGGGTTTAGTCTGGGTggtgtg                                                                                                                                               >  2:1525014/1‑234 (MQ=255)                                                                                               cGTACGAGTCATCAAACCATATGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTTCTTAACAGCAAGCTT‑GGCGGCATTCCGG‑GGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTTCCATTGAAGGGCAAATGGGCGTGAACCGCCAACGAACCTTGAAAGGTTATAGGCCGGGTtttctttcataca                                                                                                                                        >  2:1932963/1‑234 (MQ=255)                                                                                                   cGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCg                                                                                                                                     >  1:1472120/1‑238 (MQ=255)                                                                                                      gTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgc                                                                                                                                   >  1:1362995/1‑237 (MQ=255)                                                                                                       tcatcaAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCt                                                                                                                                >  1:505230/1‑239 (MQ=255)                                                                                                              aCCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCa                                                                                                                          >  1:1881658/1‑238 (MQ=255)                                                                                                                     gTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACgg                                                                                                                  >  1:876601/1‑239 (MQ=255)                                                                                                                                    ggTAATAAACAGGATGTACTGGAAAAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑GGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCtt                                                                                                    >  2:451300/1‑238 (MQ=255)                                                                                                                                          aaaCAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCggg                                                                                             >  1:1927741/1‑239 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGaa                                                                                                                                                                                       >  1:271130/1‑143 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGaa                                                                                       >  1:1004378/1‑239 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGaa                                                                                       >  1:121358/1‑239 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGaa                                                                                       >  1:246166/1‑239 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGaa                                                                                       >  1:1748989/1‑239 (MQ=255)                                                                                                                                                gATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGaa                                                                                       >  1:1506790/1‑239 (MQ=255)                                                                                                                                                          aaGAGCCAGGGGCGGCTTTCCGACCCAGAAGTCTG‑GGCGCCACCCGGG‑TAAAATTTT‑CCC‑TTG‑GCGGTAAAGAGTTTTCCCTCACACCCCGCCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCt                                                                             <  1:1767262/239‑1 (MQ=255)                                                                                                                                                          aaGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACggg                                                                                                                 >  1:661150/1‑203 (MQ=255)                                                                                                                                                          aaGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCt                                                                             >  2:1052885/1‑239 (MQ=255)                                                                                                                                                           ggagcaatgggcggcTTCCCTGCCAAGAAAGCTT‑GGCGGC‑TTCCGG‑GTAAATTTTCCCC‑TTG‑GCGGGGAGGGGTTTTCCCCCGCACCCCTACCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCt                                                                             <  1:727845/229‑1 (MQ=255)                                                                                                                                                              gCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTa                                                                           >  2:793367/1‑237 (MQ=255)                                                                                                                                                              gCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTa                                                                           >  1:1718357/1‑237 (MQ=255)                                                                                                                                                              gCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTa                                                                           >  2:412485/1‑237 (MQ=255)                                                                                                                                                               ccAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCCTCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCt                                                                        >  1:681907/1‑239 (MQ=255)                                                                                                                                                                 aaTGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCttt                                                                      >  1:38212/1‑239 (MQ=255)                                                                                                                                                                   tGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTa                                                                     >  1:159218/1‑238 (MQ=255)                                                                                                                                                                         cATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt                                                              >  1:1475573/1‑239 (MQ=255)                                                                                                                                                                         cATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCG‑CACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGt                                                              <  1:20528/238‑1 (MQ=255)                                                                                                                                                                              cTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg                                                          >  2:750771/1‑238 (MQ=255)                                                                                                                                                                               gcacgaGCAGGTTT‑GGC‑GCATTCCGGTTGAAATTTG‑CCC‑GAA‑GCGGGAATTATTGTTCCCCCACAACCCCGCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                        <  1:1098065/234‑1 (MQ=255)                                                                                                                                                                                     gCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCt                                                  >  1:672531/1‑239 (MQ=255)                                                                                                                                                                                      cAAGCTT‑GGCGGCATTCCGG‑TGGAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTAACATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTATGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCtt                                                 >  1:196140/1‑239 (MQ=255)                                                                                                                                                                                      cAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCtt                                                 >  1:1743802/1‑239 (MQ=255)                                                                                                                                                                                           gtgcgcgccATT‑CGG‑GGAAAGTTC‑CCCATTA‑GCAGTGAGGGGTTCTCCCCCACACCCCTTCACCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                                <  2:1472120/232‑1 (MQ=255)                                                                                                                                                                                                     cttccctgtaaaaTTT‑TCC‑TTAGGCCGTGAAGATTGTTCCCCCCAACCCCGCCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                      <  1:608514/229‑1 (MQ=255)                                                                                                                                                                                                       cggg‑TGAAATTTT‑CCC‑TTA‑GCCGAGAGGAGTTTTCCCCCACACCCTGTGCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg                                 <  1:1383897/237‑1 (MQ=255)                                                                                                                                                                                                            tGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCtat                             >  1:338587/1‑239 (MQ=255)                                                                                                                                                                                                              aaaGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCtata                            >  2:1685619/1‑238 (MQ=255)                                                                                                                                                                                                                   tt‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAc                       >  2:1751470/1‑238 (MQ=255)                                                                                                                                                                                                                          cttaccggagAAGTGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc                  <  1:987785/230‑1 (MQ=255)                                                                                                                                                                                                                            a‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTc               >  2:752567/1‑239 (MQ=255)                                                                                                                                                                                                                                ggTGAAGAGTGTTCCCCCCCACCCCTTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg             <  1:1932963/237‑1 (MQ=255)                                                                                                                                                                                                                                         tgtTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAc                       >  1:1353574/1‑219 (MQ=255)                                                                                                                                                                                                                                          ttttCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg  <  1:208555/238‑1 (MQ=255)                                                                                                                                                                                                                                          gtTTCCTCGCAACCCGAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAAGGAACATTGAAAGGTTATCGTCTGGATGTTCTGTCTAACACAGGCGCTTATGCATCTCACGGGCACTCAATCGCTTCTGCGGGGGGGAATAAAGTGGCTGACCTTTATCATCGTTGTGCCTACGCTTACAGTTCCAAGACCTGCTCTACCAACGTACCCTCTGCTGGTGCGAt   >  2:1436586/1‑238 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         AGGATGACTCGCGAATTACCATCGTTTCCAGCACCCAGATCCCGCACATTGTTCGCCGCGTGGTTGGTCAGGCGCTGGATATTCCCTGGTCATGCGTACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTT‑GGCGGCATTCCGG‑TGAAAGTTT‑CCC‑TTA‑GCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATG  >  NC_007779/2999581‑3000047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑