BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	3,741,162	A→T	9.2%	Y364F (TAC→TTC)	Y75_RS18545 →	adenine permease AdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	3,741,162	0	A	T	9.2%	344.1 / 3.3	137	Y364F (TAC→TTC)	Y75_RS18545	adenine permease AdeP
Reads supporting (aligned to +/- strand): ref base A (46/71); new base T (11/3); total (57/80)
Fisher's exact test for biased strand distribution p-value = 8.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGATTGTCTTTA  >  NC_007779/3740927‑3741395

    aaTTCCCGAGCATGACGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGc                                                                                                                                                                                                                                     <  2:488855/239‑1 (MQ=255)

     aTTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGGCAGGCTACGCTGCAGCTGGTGGGCTGATTTATGTTGGCg                                                                                                                                                                                                                                    >  1:276722/1‑239 (MQ=255)

       tCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTGCGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTg                                                                                                                                                                                                                                  <  1:1088435/239‑1 (MQ=255)

          cgcgCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCtg                                                                                                                                                                                                                               >  1:741877/1‑239 (MQ=255)

                 gAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACtt                                                                                                                                                                                                                        <  1:557482/239‑1 (MQ=255)

                             gTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTAAGCCGCAGCTGGTGCGCGGGTTTTCGTTGGCGTGCTGATGACTTCCCGTCTGGGAc                                                                                                                                                                                                            >  1:683512/1‑239 (MQ=255)

                               aTGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGGCCGGCCACGCGGCCGGTTGGGGGCTGGTTTTCGTTGGCGTGCTGGTGGCTTCCAGTCTGgcgcgc                                                                                                                                                                                                          >  1:157877/1‑239 (MQ=255)

                                tGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGTTTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCAcgcg                                                                                                                                                                                                         <  1:1008285/239‑1 (MQ=255)

                                    gACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGaa                                                                                                                                                                                                     <  1:171383/239‑1 (MQ=255)

                                      cAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGAGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGGCTTTCAGTCTGTCACGCGTGa                                                                                                                                                                                                      >  1:1607827/1‑236 (MQ=255)

                                      cAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGAGGGGCCAGGCTCCGCTGCAGGTGGTGCGCTGATTTACGTTGGCGTGCTGCTGACTTCTAAGCTGGCACGCGTGa                                                                                                                                                                                                      >  1:539736/1‑236 (MQ=255)

                                        gTATCTCTTCCGTGACCGGTTCGTTTATCGGTACGTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTgg                                                                                                                                                                                                 <  2:859097/239‑1 (MQ=255)

                                          aTCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  1:1231040/239‑1 (MQ=255)

                                          aTCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  1:512443/239‑1 (MQ=255)

                                          aTCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  1:574768/239‑1 (MQ=255)

                                            ctctTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCCGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTTGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAgg                                                                                                                                                                                             >  1:885103/1‑239 (MQ=255)

                                              ctTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGAt                                                                                                                                                                                           <  2:927775/239‑1 (MQ=255)

                                              ctTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGAt                                                                                                                                                                                           <  1:1852814/239‑1 (MQ=255)

                                               ttCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATc                                                                                                                                                                                          <  1:1259001/239‑1 (MQ=255)

                                                 ccGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGTGCCCGGCCACGCTGCAGCTGGTGCGCCCGTTTTTGTTGGGGGGCTGGTGACTTTCAGGCCTGCACGCGTGCAATGGCggggactt                                                                                                                                                                                        >  2:832106/1‑234 (MQ=255)

                                                 ccGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTgg                                                                                                                                                                                                 <  1:659044/230‑1 (MQ=255)

                                                   gTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTTGTGAAAGGCTAGGCGGCAGCTGGGGCGCTGTTTTTCGTTGGGGGGCGTGTGGATTCCACTATGGCACGCGTGAACCGGCAgggtatt                                                                                                                                                                                        >  2:605845/1‑232 (MQ=255)

                                                        cGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAAt                                                                                                                                                                                 <  1:616199/239‑1 (MQ=255)

                                                           ttCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTTGGGCCAGGGTACGCTGCACCCTGGGCGCGGGTTTTCGCTTGCGTGGTGTGGGATTCCCGTCTGGCACGCGGGAGCTCGCAGGGTCtttcctttctct                                                                                                                                                                              >  2:659044/1‑229 (MQ=255)

                                                                ttATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCg                                                                                                                                                                         <  2:245693/239‑1 (MQ=255)

                                                                   tCGGTCCTTTGTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCg                                                                                                                                                                      <  2:1512983/239‑1 (MQ=255)

                                                                     ggTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGGGCCCGGCGACGCTTGAGCGGGGGGGCTGGTTTTCGGTTGCGGGGTGGTGACCTCCAGGCCGGGGCGCCGGGACTGGGAGGGGCTTTCTGGAACTGTTTCGGCGtt                                                                                                                                                                    >  2:656692/1‑239 (MQ=255)

                                                                            cttcCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGTGGCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGGTGGCGTGCTGGTGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTTTTCCGGCGGTTATTAc                                                                                                                                                              >  1:447564/1‑238 (MQ=255)

                                                                                 gTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGt                                                                                                                                                        >  1:847383/1‑239 (MQ=255)

                                                                                 gTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGGTATCTTTCTGTCGCC‑GCTGGCGGGGGGGGTGGCAGGCGACGCCGCAGCTTGTGCGCCGGTTTTCGTTGGCGTTGTGATGACTTCCAGTATCGCACGCGGTGACTGGCAGGTACTTACTCAATCTTTTTCGGCGGTTTTTCtcgccgg                                                                                                                                                        >  2:1326460/1‑238 (MQ=255)

                                                                                  ttACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGGTTTTTACCCCCGtg                                                                                                                                                       >  1:1662532/1‑239 (MQ=255)

                                                                                        cTTATTGTGAGCCCTCTTCCGGCGTTTCGGTTGGCGGTGTTACCGTCCTGACGGCTGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGa‑tgatg                                                                                                                                                <  2:749739/239‑1 (MQ=255)

                                                                                                 aGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGTGCCAGGCTACGCTGCAGCTGGGGCGCTGATTTTCGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCa                                                                                                                                         >  1:347977/1‑237 (MQ=255)

                                                                                                  gTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCCGTCAGCt                                                                                                                                      >  1:1552664/1‑239 (MQ=255)

                                                                                                  gTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  2:55378/183‑1 (MQ=255)

                                                                                                  gTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               >  1:55378/1‑183 (MQ=255)

                                                                                                       ctTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCg                                                                                                                                 <  1:1173212/239‑1 (MQ=255)

                                                                                                        ttCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  1:1712414/177‑1 (MQ=255)

                                                                                                                tATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGTGCCAGGCTACGCTGCAGCTGGGGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGGTCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGGGA‑TGATGCCGTTCAGCTTCTCGATTACCGaa                                                                                                                        >  1:1127647/1‑239 (MQ=255)

                                                                                                                  tCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGGTGGGGCCCGGGTAAGCTGCAGCTGGGGCGCTGCTTTTCGTTGGGGTGCTGGTGGTTTTCAGGCTGGCACGGGGGGGCTGGCCGGGGTTTTCCGTATCTGTTTCGTGGTTTATTTTCGCCGTGATTGTTGCCGTTC‑GCTTTTCTGTTATCGAAgg                                                                                                                      >  2:428296/1‑239 (MQ=255)

                                                                                                                    ggTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCa                                                                                                                                                                                               <  1:627352/165‑1 (MQ=255)

                                                                                                                     gtggggggTCGTCCCGTTCTGCGGGCAGGGGTTTTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTCCGTTGGCGTGCTGATGACTTCCAGTCTGGCCCGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTa                                                                                                                    <  2:1205222/232‑1 (MQ=255)

                                                                                                                        ggCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTTCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGGAGGGATcgc                                                                                                                >  1:508936/1‑239 (MQ=255)

                                                                                                                          gggTCGTACCGGTCTGCCGGCAGGGGTTGTTGGTCTGCTGTTCCTGCGGGTTTTCTTTCTGTCGCC‑GCTGGCGGGGATGGTCCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGGGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATcgcg                                                                                                               <  1:1820843/237‑1 (MQ=255)

                                                                                                                             tCGTTCCGGTCTGGCGGCGGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTGCGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGATTCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTg                                                                                                            <  1:109266/238‑1 (MQ=255)

                                                                                                                             cggtccggggcTGACGGGGGTGGTTGTTGGCTTGCTGTTCCTCTTGGTTAGTTTTCTGTGGCC‑GCGGGGGGGGGTGGTCCCCGGTTACCCTGCTGCGGGTGGCCTGGTTTCCGTTGGCGTTCTGTTTCCTCCCGGTCTGCCACGCTTGACTTGGCAGTGTCTTATTCAATTTGTCCCGGCTTTTTTTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTg                                                                                                            <  2:732935/228‑1 (MQ=255)

                                                                                                                                tcccgttttGACGTCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GTGGGCGGGGATGGTGCCAGGCTACGCTGCAGCGGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTCCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:1584265/231‑1 (MQ=255)

                                                                                                                                 tccGGTCTGACGGCAGTGGTTGTTGGTCTGCTTTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTCCGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCTTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:517251/237‑1 (MQ=255)

                                                                                                                                 gccGGTCTGGCGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:1061821/237‑1 (MQ=255)

                                                                                                                                 aCCGGTCTGGCGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCG‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:35899/238‑1 (MQ=255)

                                                                                                                                 aCCGGTCTGCCGGCAGTGTTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGGAAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:1908083/238‑1 (MQ=255)

                                                                                                                                 aCCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGCCTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:398075/238‑1 (MQ=255)

                                                                                                                                  ccgggttagaggCTGTGGTTGTTGGGCTGCTTTTCCTGCTGGTGATGTTTCTGGGGCC‑GCTGGGGGGGAGGGGGCCAGGCTTGTTTGCAGTTGGTGGGCTGATTTTCTTGGGTGTGCTGATGACTTCCAGTCTGGCACGGGTAACCTGTCAGTTTCTTACTGAATCTGTTCCGGCGTTTATTTCCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCttt                                                                                                      <  2:285788/229‑1 (MQ=255)

                                                                                                                                  ccGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCttt                                                                                                      <  1:656692/239‑1 (MQ=255)

                                                                                                                                  ccGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCGGGTTATCTAGCGGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTCCGCTGCAGCTGGTGCGTTGATTTACGTTGGCGTGCTGATGACTCCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCttt                                                                                                      <  1:395141/239‑1 (MQ=255)

                                                                                                                                  ccGGTCTGACGGCAGGGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCttt                                                                                                      <  1:605845/239‑1 (MQ=255)

                                                                                                                                     gTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTTGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGGTCTTACTGAATCTGTTTCGGCGTTTATTACCGCCGTGa‑tgatg                                                                                                                                                >  1:1119441/1‑194 (MQ=255)

                                                                                                                                       cTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTAtctc                                                                                                 <  1:1002078/239‑1 (MQ=255)

                                                                                                                                           cGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTa                                                                                              <  1:199987/238‑1 (MQ=255)

                                                                                                                                               aGTGGTTGTTGGTCTGCTGTCCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGGGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGc                                                                                          <  1:1348863/238‑1 (MQ=255)

                                                                                                                                                 tGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTTTCTCCTACTGCGtg                                                                                       >  1:741941/1‑239 (MQ=255)

                                                                                                                                                  ggTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGGGGGGATGGGGCCAGGCTACGCTGCAGCTGGTGCGCTAATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGGGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACAGCGtga                                                                                      <  1:65210/239‑1 (MQ=255)

                                                                                                                                                    ttgttgGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGGGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGtgatg                                                                                    >  1:1647722/1‑239 (MQ=255)

                                                                                                                                                         ggTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGaaaa                                                                                >  1:1319600/1‑238 (MQ=255)

                                                                                                                                                          gtctgtgttgccTGCTGGTTGTCTTTCGGTCGCG‑GCGGGGGCGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGGTGTTTTAGTTTGGCGTGCTGATGACTTCCAGGCTGGCCTGGGTGAACGGGAAGGTTCTTGCTTAATCTCTCCCGGCGTTTTTTCCCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAAt                                                                               <  2:1686045/228‑1 (MQ=255)

                                                                                                                                                            ctgctgTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGAGGGTGCCAGGCTCCGCTGCAGCTGGTGCGCTGATTTACGTTGGGGTGCTGATGACTTCCAGTTTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGGGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCgg                                                                            <  1:754463/239‑1 (MQ=255)

                                                                                                                                                                            ttttttttCTGTCGCC‑GCGGGCGGGGATGGTGCCAGGCTCCGCGGCAGCGGGTGCGCTGATTTACGTGGGCGGGCTGAGGTCTTCCAGTCGGGCACGCGTGAACTGGCAGGATCTTACTGATTCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             <  2:1067489/233‑1 (MQ=255)

                                                                                                                                                                            ttATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGTTGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTCCCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             <  1:1361889/238‑1 (MQ=255)

                                                                                                                                                                            ttATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGGGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGGATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGt                                                            >  1:700087/1‑239 (MQ=255)

                                                                                                                                                                            ttATCTTTCTGTCGCC‑GCGGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             <  1:822640/238‑1 (MQ=255)

                                                                                                                                                                            ttATCTTTCGGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGCCTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             <  1:1708321/238‑1 (MQ=255)

                                                                                                                                                                             ttttttttTGTCGGCGGCTGGGGGGGAGGGGGCCGGGTTCCGCGGCGGCTGGTGCCTTGATTTCCGTTGGGGGCCTGATATCTCCCAGTCTGCCCCGGTTGAATTGGCGGGATTTTACTGATTCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             <  2:1376234/234‑1 (MQ=255)

                                                                                                                                                                                     tGTCGCC‑GCTGGCGGGGGTGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAg                                                    >  1:253401/1‑238 (MQ=255)

                                                                                                                                                                                          cc‑gcTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGGGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGt                                               >  1:886261/1‑238 (MQ=255)

                                                                                                                                                                                           c‑gcTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTg                                              <  1:1689151/238‑1 (MQ=255)

                                                                                                                                                                                                        tGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGtt                                 >  1:1662780/1‑239 (MQ=255)

                                                                                                                                                                                                         ggTGCCGGGCTACGCTGCAGCTGGTGCGCTGATTTACGTGGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTg                                <  1:803995/239‑1 (MQ=255)

                                                                                                                                                                                                         ggTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGCCTTCCATTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTg                                <  1:145324/239‑1 (MQ=255)

                                                                                                                                                                                                          gTGCCGGGTTCCGCTGCAGTTGGTGCGTTGTTTTGCGTGGGGGTGCTGATGCTTCCCTTTCTGGCCCTCGTGACTTGGCAGTATCTTACTGAATCTTTTCGGGTGTTTTTTTCCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTg                                <  2:1662532/238‑1 (MQ=255)

                                                                                                                                                                                                           tGCCCGGCTACGCTGCAGCTGGTGCGCTGCTTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGCACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTCTTACCGCCGTGA‑TGATGCCGTTCCGCTTCTCGATTCCCGCAGGTCTCGCGCTGGGCTTTATCTCCTACTGCTTGATGACACTCGGTCCCGGCCGTCTGCGTGATTTAAACCCGTTCGTCATCCTCGTTgc                               >  2:25851/1‑238 (MQ=255)

                                                                                                                                                                                                              cAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCgct                            <  1:1213926/238‑1 (MQ=255)

                                                                                                                                                                                                              cAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCgct                            <  1:1213914/238‑1 (MQ=255)

                                                                                                                                                                                                                ggCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCgct                            >  1:1078174/1‑236 (MQ=255)

                                                                                                                                                                                                                   tACGCGGCAGCGGGTGCGCGGATGTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCGGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGt                       <  1:116607/238‑1 (MQ=255)

                                                                                                                                                                                                                   gccgcgGCAGTGGGTGCGTTGATTTCCGTGGGCGGGCGGATGCTTTCCAGTCTGGCACGCGGGAACTGGCGGGTTCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGt                       <  2:447564/236‑1 (MQ=255)

                                                                                                                                                                                                                    tcggtgcggggggTGCGCTGATTTCGGTTGGTGTGGTTTGTCCTGCCTGTGTGGTACGCGGGAGCGGGCAGGATCGTATCGAATCTTTTCGGGCTTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATGACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCAGCGTTGCGCTGCTGttt                     <  2:1912773/228‑1 (MQ=255)

                                                                                                                                                                                                                     cgcgggaGCTGCTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGATCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGGCGGGCTTTATCTCCTCCTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCAGTGCGTAATCATCGTGGCGCTGCTGTTTa                    >  2:1231557/7‑239 (MQ=255)

                                                                                                                                                                                                                         gCAGCTGGTGCGCTATTTTACTTTGGCTTGCTGATGATTTCCAGTCTGACGCGCGTGAGCTGGCGGGATCTTACTGAGTCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCt                <  1:1451978/239‑1 (MQ=255)

                                                                                                                                                                                                                          cAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTg               >  1:185825/1‑239 (MQ=255)

                                                                                                                                                                                                                           aGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCg                                                             >  2:1785046/1‑192 (MQ=255)

                                                                                                                                                                                                                           aGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTg               <  1:650897/238‑1 (MQ=255)

                                                                                                                                                                                                                           aGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGa              >  1:1086077/1‑239 (MQ=255)

                                                                                                                                                                                                                            gCTGGGGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTGCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGaa             >  2:1500509/1‑239 (MQ=255)

                                                                                                                                                                                                                              tGGTGCGCTGATTTACGTGGGCGTGCTGATGCCTTCCAGTCTGGCACGCGTGACCTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGa           <  1:1685374/239‑1 (MQ=255)

                                                                                                                                                                                                                               ggTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        <  1:959753/145‑1 (MQ=255)

                                                                                                                                                                                                                               ggTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCt                                                                                                        >  2:959753/1‑145 (MQ=255)

                                                                                                                                                                                                                                gTGCGCTGATGTAGGTTGGCGTGCTGATGATTTCCACTGTGGCAGGCGTAGTCTGGCAGTATCTTCTTTATTTTTTTCCGGTGTTTTTTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGAt          <  2:1734429/238‑1 (MQ=255)

                                                                                                                                                                                                                                 tGCGTTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGGGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGAtt         <  1:414434/238‑1 (MQ=255)

                                                                                                                                                                                                                                    gcTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGATTGTCt     >  1:1947736/1‑239 (MQ=255)

                                                                                                                                                                                                                                       gATTTACGTTGGCGTGCTGATGCCTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTCTTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGACGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGATTGTCTTTa  >  1:1306725/1‑239 (MQ=255)

GGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGTTACGGCTTATATTGAGTCCTCTTCCGGCGTATCGGTTGGCGGTCGTACCGGTCTGACGGCAGTGGTTGTTGGTCTGCTGTTCCTGCTGGTTATCTTTCTGTCGCC‑GCTGGCGGGGATGGTGCCAGGCTACGCTGCAGCTGGTGCGCTGATTTACGTTGGCGTGCTGATGACTTCCAGTCTGGCACGCGTGAACTGGCAGGATCTTACTGAATCTGTTCCGGCGTTTATTACCGCCGTGA‑TGATGCCGTTCAGCTTCTCGATTACCGAAGGTATCGCGCTGGGCTTTATCTCCTACTGCGTGATGAAAATCGGTACCGGACGTCTGCGTGATCTAAGCCCGTGCGTAATCATCGTTGCGCTGCTGTTTATCCTGAAGATTGTCTTTA  >  NC_007779/3740927‑3741395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`