BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	4,463,104	A→C	14.8%	T156P (ACC→CCC)	pmbA →	metalloprotease PmbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,463,104	0	A	C	14.8%	372.4 / 11.2	177	T156P (ACC→CCC)	pmbA	metalloprotease PmbA
Reads supporting (aligned to +/- strand): ref base A (71/73); new base C (11/20); total (82/95)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TAGCGCATCATCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATC  >  NC_007779/4462869‑4463333

 aGCGCATCATCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCa                                                                                                                                                                                                                                       <  1:565631/238‑1 (MQ=255)

     catcatCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATAACCAATAcc                                                                                                                                                                                                                                  >  1:1847902/1‑239 (MQ=255)

              ccGATTTAAGCCCGCAGGCCATTGCCCGTACTTGACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑GCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGA‑GCAGAACAGGCGGC‑ATTGCAGGCGGCCAAACGCATCACCAATACCGAAGGTG‑Gc                                                                                                                                                                                                                        <  2:1890283/239‑1 (MQ=255)

                    tAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCCGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAAAAGGCGGG‑CTTGCCGGCGGGCAAAAACCTCCCCCAAACCCCAGGGG‑GCAGC‑ttt                                                                                                                                                                                                                 >  2:1785600/1‑239 (MQ=255)

                     aaGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑a                                                                                                                                                                                                               <  1:1409198/239‑1 (MQ=255)

                         ccGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCCCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AAc                                                                                                                                                                                                             <  1:1827829/237‑1 (MQ=255)

                           gCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGGCAAACGCATCACCCATACCCAAAGGG‑GCCGC‑CTT‑aaaaac                                                                                                                                                                                                          >  2:352357/1‑234 (MQ=255)

                           gCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGcc                                                                                                                                                                                                         >  1:1038060/1‑239 (MQ=255)

                                       ccGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAAACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCa                                                                                                                                                                                             <  1:1954356/239‑1 (MQ=255)

                                         gTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGGGTCaaa                                                                                                                                                                                           >  1:1031432/1‑239 (MQ=255)

                                           aCTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGt                                                                                                                                                                                         >  1:1326181/1‑239 (MQ=255)

                                              gTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCCCCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCCGGCAAAAGCCTCACCAATACCGAAGGGG‑GGAGC‑TTT‑TACAACCACCAAGGTGGCAAAAtttt                                                                                                                                                                                      >  2:239995/1‑239 (MQ=255)

                                              gTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACCGCCACTACGGTGTCAAAGattt                                                                                                                                                                                      >  1:305617/1‑239 (MQ=255)

                                              gTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAAACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGGCAAACGCATCACCAATACCGAAAGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGtttt                                                                                                                                                                                      >  1:280372/1‑239 (MQ=255)

                                               tACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACCGGCGGC‑ATTGCCGGCGGACAAACCCATCACCCATACCGAAGGGG‑GCAGC‑TTTCAAACACCAC‑ACGGGGTCAAACttttt                                                                                                                                                                                     >  2:1165375/1‑239 (MQ=255)

                                               tACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGttttt                                                                                                                                                                                     >  1:1158598/1‑239 (MQ=255)

                                               tACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGGGTCAAAGttttt                                                                                                                                                                                     >  1:745045/1‑239 (MQ=255)

                                               tACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AAAGCTGCTGGCCTTTGACGCACCAGATCTCGAATTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGGAGCCATTGAAATGGCGGCCCGC‑GCCGAACAGGCGGC‑AATGCATGCGGGGAAAAGCATGCCCACTACACAAAGTG‑GAAGC‑GTT‑AACCGCCACCACCGGGTCAAAGttttt                                                                                                                                                                                     >  2:419159/1‑239 (MQ=255)

                                                    ggtgggcgtgATATTGCCCGTTTTACCTCG‑CAGACTCCCTGTGCCGGGGTGGCAAACAA‑AGGGAGGTGGCCCTTTGAGCCCCCACATCCGGGCTTG‑TCCCCCCTGGGGGAGTTTCCCCC‑GGATGAAGCATTGGAACGGGCGGCCCGC‑GCGGAACGGGCGTA‑TTGGCGGGGGGACAAACGCATCACAAATCCGGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCa                                                                                                                                                                                 <  2:1474181/229‑1 (MQ=255)

                                                    gcggcgCTGGATATTGCCCGTTATACCTCGCCACA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCCCCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGGCAAAAGCCTACCCCCAACCGAAGGTG‑GCCGC‑GTT‑AAAAGCCACCACGGCGTCAACGTTTTTTGcc                                                                                                                                                                                 >  2:597087/1‑237 (MQ=255)

                                                     cggcgCTGGTTATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCaa                                                                                                                                                                                <  1:634582/238‑1 (MQ=255)

                                                         gcTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGcc                                                                                                                                                                           >  1:598398/1‑239 (MQ=255)

                                                         gcTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGc                                                                                                                                                                            >  1:186836/1‑238 (MQ=255)

                                                         gcTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGGGTCAAAGTTTTTGGCAACAGc                                                                                                                                                                            >  1:178143/1‑238 (MQ=255)

                                                         gcTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCCGGCCTTTGACGCACCCGATCTCGACTTGTTCCACCCTGCGGAAGGTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAAAAGGGCGC‑CTTGCAAGCGGAACAACCCATCCCCCATACCCAAAGGG‑GCCGCATTT‑AAAGGCCTCAACGG‑GTCAAAGCTTTTTGCCCCAGc                                                                                                                                                                            >  2:236662/1‑238 (MQ=255)

                                                         gcTGGATATTACCCGTTATACCTCGCCAGA‑ACCCTGTGCCGGCGTGGCCGACAA‑AAAGCTGCTGGCCTTTGACGCCCCCGATCTCGACCTGTTCCACCCCGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCCGAACAGGCGGC‑ATTGCAGGCGGGCAAACGCATTAACAAAACCGAAGGGG‑GGAGC‑CTT‑AAAAGCCCCCAACGGGGCAAAGTTTTTgggacacgc                                                                                                                                                                            >  2:746916/1‑231 (MQ=255)

                                                           tGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGGGTCAAAGTTTTTGGCAACAGCCAc                                                                                                                                                                         >  1:247839/1‑239 (MQ=255)

                                                               ttttccccTTTATACCGCCCCAGA‑‑CCCCGGGGCGGGGTGGGCAACAAGAGCGCTGCTGGCCTTTGACGCACCAGATCTGGACTTGTCCCCCCCTGGGGAGTTTT‑CCCC‑GGATGAAGCCATGGAACGGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATCCCGAGGGGG‑GCAGT‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGc                                                                                                                                                                      <  2:1851135/233‑1 (MQ=255)

                                                               tatcgcggtttATCCCCCGCCGGC‑TCCCGGGGCGGGGGGGGCATACAA‑AGAGCGGCTGACCTTGCACGCCCCCGCTCGCGCGTTGTCCCACCCTGCGGAAATTC‑CCCC‑GGTTAAAGCCATTGACCGGGCGGCCCGC‑GCAAAAGGGGCGGC‑ATTGCAGGGGGCAAAACCCTTCCCCAATACGGAGGGTG‑CCTGC‑TTA‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCa                                                                                                                                                                     <  2:612878/230‑1 (MQ=255)

                                                               tatTGCCCGTTATCCCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGTCGCACCAGATCTCGATTTGTTCCGCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATGTAACGGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCACCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCa                                                                                                                                                                     <  1:292351/239‑1 (MQ=255)

                                                               tatTGCCCGTTATCCCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCa                                                                                                                                                                     <  1:431439/239‑1 (MQ=255)

                                                               tatTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGc                                                                                                                                                                      <  1:980121/238‑1 (MQ=255)

                                                                 gtccccGTCATCCCCCCCCAGC‑CCCCTGGCCCGGCGTGGCACACAA‑AGAGCGGCTGCCCTTTGGCCCACCGGATCTCGACTTGCTCCCCCCGGGGGAATTTC‑CCCC‑GGAGAAAGCCATTAAACGGGCCCCCCGC‑GAGAAACGGGCGGT‑ATTGCGGGCGAAAAAACGCCCCACCAATACCGAGGGGG‑GCAGC‑TTA‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATg                                                                                                                                                                   <  2:1870646/236‑1 (MQ=255)

                                                                    cccGTTTTACCTCGCCAGA‑CCCCTGTGCCGGGGTGGCAAACAA‑AGGGTTGCGGCCCTTTGACGCACCAGATCTCGACTGGTTCCCCCCTGCGGAGGTTT‑CCCC‑GGAGGAAGCCATTGACCGGGCGGCCCGC‑GCAGAACGGGCGGC‑ATTGCAGGCGGACAAACGCATCCCCAATCCCGAAGGGG‑GCAGT‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTg                                                                                                                                                                <  2:1806858/239‑1 (MQ=255)

                                                                     ccGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCGCGACTTGTTCCACCCGGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACGGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTg                                                                                                                                                                <  1:372665/238‑1 (MQ=255)

                                                                     ccGTTATACCTCGCCAGA‑TCCCTGGGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATCCCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTg                                                                                                                                                                <  1:1081897/238‑1 (MQ=255)

                                                                       gttaccccTCGCCAGA‑TCCGTGGGCGGGGGGGGCGAACAA‑AGGGCTGCTGGCCTTTGACCAACCGGATCTGGACTGTTCCCACCCGGCGGAAGTTC‑CCCC‑GGAGGAAGCCATGGAACGGGCGGCCAGG‑GAAGAACAGGGGGC‑ATTGCAGGGGGACAACCGCACCACCAAAACAGAGGGTG‑GCAGT‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAg                                                                                                                                                             <  2:1060310/233‑1 (MQ=255)

                                                                       gTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCCCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAg                                                                                                                                                             <  1:817886/239‑1 (MQ=255)

                                                                       gTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCCGAACAGGCGGC‑ATTGCAGGCGGGCAAAAGCACCACCAATACCGAAAGGG‑GCCGC‑CTT‑AAAAGCCCCTACGGGGTCAAAGGTTTTTGCAAAAGCCCACGCACGTTGCAg                                                                                                                                                             >  2:1547337/1‑239 (MQ=255)

                                                                       gTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAg                                                                                                                                                             >  1:753182/1‑239 (MQ=255)

                                                                         tataCCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACCAACGCATCCCCCATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCCCCACGGTGTCAAAGTTTTTTGCAACACCCACGGCCTTTTggcggg                                                                                                                                                           >  2:1853997/1‑234 (MQ=255)

                                                                            aCCTCGCCAGA‑TCCCTGTGCCGGCGCGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACGGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTa                                                                                                                                                        <  1:1266651/239‑1 (MQ=255)

                                                                                cGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACg                                                                                                                                                                                                   >  2:1522265/1‑192 (MQ=255)

                                                                                cGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACt                                                                                                                                                      >  1:1806858/1‑237 (MQ=255)

                                                                                        tccttggcccGGCGTGGAAGAGAA‑AGGGTGGCTGCCGTTGGCCCCACCAGACCTCGACTTTTCCCCCCCCGCGAAAGTTA‑CCCC‑GGAGGAGGCCATTGAACGGGGGGCCCGC‑GCAAAAAGGGCGGT‑GTTGCAGGCGGAAAACCGCATCACAAATACGGAGGGTG‑GGCGT‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAAcgc                                                                                                                                             <  2:1746196/231‑1 (MQ=255)

                                                                                              tgCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑TAc                                                                                                                                                                                                             >  2:283305/1‑169 (MQ=255)

                                                                                              tgCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACCCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCCATACGGTGTCAAAGTTTTTTGCAACAGCCACCGCAAGTTGCAGGGGTACTg                                                                                                                                                     >  2:1152426/1‑225 (MQ=255)

                                                                                                  ggCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGCG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGCTGCAGGGTTAATGCTCAACGCGTCATTCGc                                                                                                                                    >  1:797788/1‑238 (MQ=255)

                                                                                                   gCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGc                                                                                                                                    >  1:905823/1‑237 (MQ=255)

                                                                                                   gCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAAAGCATCCCCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACCACGGGGTCCAAGTTTTTGGCAAACGCCCCGGCATGTTCACGGGTTATTGCGCAACCAGGCCTTCGct                                                                                                                                   >  2:1930933/1‑238 (MQ=255)

                                                                                                        gCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCa                                                                                                                              >  1:265788/1‑238 (MQ=255)

                                                                                                        gCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACCCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCCAAGTTTTTTGCAACAGCCACGGCCTGTTGCAGGGGTACTGCTCAACGCGTCATTCGCCCTCCa                                                                                                                              >  2:1016913/1‑238 (MQ=255)

                                                                                                             cAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGATTTTGGCAACAGCCACGGCATGTTGCAGGGTTAATGCTCAAGGCCTCATTCCCCCCCCAGCtggg                                                                                                                        >  2:1901103/1‑237 (MQ=255)

                                                                                                              aa‑aGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCCCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCCCCAATCCCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTgtgt                                                                                                                       <  1:954705/239‑1 (MQ=255)

                                                                                                               aaggagCTGGGGCCCTTTGACGCCCCAGCTCGCGA‑TTGTTCCCCCCCTGGGGAGTTT‑CCCCGGGATAAAGCCATAGAACTGGCGGCCCGC‑GCAGAACGGGCGGC‑ATGGCAGGCGGAAAAACGCATCCCCAATACGGAGGGTG‑GCAGT‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTgtgt                                                                                                                       <  2:1339312/236‑1 (MQ=255)

                                                                                                                   agCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAAtt                                                                                                                   >  1:1509109/1‑239 (MQ=255)

                                                                                                                       gctgGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCg                                                                                                               >  1:934948/1‑239 (MQ=255)

                                                                                                                        ctgGCCTTTGACGCACCAGATCTCGACTTGTCCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCg                                                                                                               <  1:547684/238‑1 (MQ=255)

                                                                                                                         tgGCCTTTGACGCACCAGATCTCGACTTTTTCCCCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATCCCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCgaa                                                                                                             <  1:557526/239‑1 (MQ=255)

                                                                                                                              tttGCCGCACCAGATCTCGACTTGTCCCCCCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCCCCAATCCCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGaaaa                                                                                                        <  1:1147173/239‑1 (MQ=255)

                                                                                                                              tttGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGaaaa                                                                                                        <  1:1109565/239‑1 (MQ=255)

                                                                                                                                tGACGCACAAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGT‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATg                                                                                                      <  1:1901103/239‑1 (MQ=255)

                                                                                                                                  aCGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGc                                                                                                    >  1:1004949/1‑239 (MQ=255)

                                                                                                                                  aCGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑AGTGCAGGCGGACAAACGCATCACCAATATCGAAGGTG‑GCAGC‑TTT‑TACAGCCCCGAAGGTGTCAAAGGTTTTTGCAACAGCCACCGGATGATTATGGGTAACTGCACGAGGCGTTCTTCCCATTCCAGCTGTCTAATTTCCgtatgaaacgg                                                                                                     >  2:1631172/1‑228 (MQ=255)

                                                                                                                                     cACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGat                                                                                                 >  1:198761/1‑239 (MQ=255)

                                                                                                                                           aTCTCGCCTTGCCCCCCCGGGCGAAGGTCT‑CCCG‑GGGTGAGGCCATGGAACTGGCGGCCAGC‑GAAGAACGGGGGGC‑ATTGCGGGGGGAAAAACGCATCACCAATCCGGAGGGTG‑GCATC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAg                                                                                           <  2:753182/239‑1 (MQ=255)

                                                                                                                                           aTCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCTGC‑ATTGCAAGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTCCGGCGTCAAAGTTTTTGGCCACAGCCACCGCATGTTGCAGGGGTACTGCCCAACGCGGCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGTCGGTATGGaa                                                                                           >  2:1403345/1‑238 (MQ=255)

                                                                                                                                            tctcGCCTTCTTCCACCCTGCGCAAGTTC‑CCCC‑GGATGCAGCCATGCAACTGGCCCCCCCC‑GCAGAACA‑GCGGCAATTGCAGGCGGACAAACGCATCCCCAATCCCGAAGGTG‑CCACC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGc                                                                                          <  1:1025168/239‑1 (MQ=255)

                                                                                                                                                gACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCa                                                                                                                                                                                             >  2:1636851/1‑136 (MQ=255)

                                                                                                                                                gACTTGTTCCACCCTGCGGAAGATT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑TCAGAACAAGCGGC‑ATTACCGGCCGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACCGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCCTTTTTCGCCTGCCAGCTGTTGTATTGCCGAAGAAAATGGCGATAAGGCGCGGGa                                                                                      >  2:867941/1‑239 (MQ=255)

                                                                                                                                                  ttggTTCCCCCCGTCGGAGGTTT‑CCCC‑GGGTAAAGCCATGCACCGGGCGCCCCGC‑CCAGACGGGGCGGC‑ATCGGCGGCGGACAAGCCCATAACAAATCCCGAGGGTG‑GCACC‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAtt                                                                                    <  2:1826025/236‑1 (MQ=255)

                                                                                                                                                  cttttcccccccGGCGGAGTTTC‑CCCC‑GGATGACGCTAGTGAGCGGGCGCCCCCC‑CCAGACCAGGCGGC‑TTGGGAGGCGGCAAACCTAATCACAATCCCCGAGGGGG‑GCAGC‑TTT‑GACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAt                                                                                     <  2:745045/229‑1 (MQ=255)

                                                                                                                                                  cTTGTTCCCCCCGGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAt                                                                                     <  1:1853997/238‑1 (MQ=255)

                                                                                                                                                  cTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAtt                                                                                    <  1:1785600/239‑1 (MQ=255)

                                                                                                                                                  cTTGTCCCCCCCTGCGGAAGTTC‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGAt                                                                                     <  1:1483728/238‑1 (MQ=255)

                                                                                                                                                    tGTTCCCCCCGGCGGAATTTT‑CCCC‑GGATAAAGCCATTGACCTGGCGGCCCGC‑GCAGACCAGGCGGC‑ATTGCAGGCGGACAAACGCATCACAAATACCGAAGGTG‑GCAGT‑TTA‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTAc                                                                                  <  1:1519027/239‑1 (MQ=255)

                                                                                                                                                    tGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCa                                                                                                                                                                          <  1:1728730/151‑1 (MQ=255)

                                                                                                                                                     gTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCCATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTCCGGTGTCAAAGTTTTTGGCAACCGCCACGGCCTGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCCCCAGCTGGGTAATTGCCGAAGAAAATGGCGATATGGAGCGGGATTAc                                                                                  >  2:1423218/1‑238 (MQ=255)

                                                                                                                                                     gTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGGG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTAc                                                                                  >  2:1234951/1‑238 (MQ=255)

                                                                                                                                                      ttCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACg                                                                                 >  1:1204534/1‑238 (MQ=255)

                                                                                                                                                        ccccccTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCCCCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCt                                                                              <  1:1547337/236‑1 (MQ=255)

                                                                                                                                                        ccccccTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATCCCGAGGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCt                                                                              <  1:1648750/236‑1 (MQ=255)

                                                                                                                                                                cGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATCCCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCAt                                                                       <  1:1816800/238‑1 (MQ=255)

                                                                                                                                                                cGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCAtt                                                                      >  1:777888/1‑239 (MQ=255)

                                                                                                                                                                               ggATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGa                                                         >  1:12063/1‑239 (MQ=255)

                                                                                                                                                                                 aTGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCAGTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATTTGGAGCGGGATTACGCCTACACCATTGGTCGTGCGATGAGc                                                       >  2:256993/1‑239 (MQ=255)

                                                                                                                                                                                     aGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCCCCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGAt                                                    <  1:19401/238‑1 (MQ=255)

                                                                                                                                                                                     aGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGAt                                                    <  1:1422500/238‑1 (MQ=255)

                                                                                                                                                                                          ttgaaacgggcgcCCGCGGCAGAGCGGGGGGC‑ATTGCAGGCGAACAACCGCTTCCCAAATCCCGAGGGTG‑GCAGC‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCa                                               <  2:198761/228‑1 (MQ=255)

                                                                                                                                                                                            gAACTGGGGGCCCGC‑GCAGACCAGGCGGC‑ATTGCAGGGGGACAAACGCATCCCCAATACCTAGGGGG‑GAGGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAAc                                            <  1:617595/239‑1 (MQ=255)

                                                                                                                                                                                                 ggcggcCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGc                                                                                          >  2:148868/1‑188 (MQ=255)

                                                                                                                                                                                                         gc‑gcAGAACAGGCGGC‑TTTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGT‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGtt                               <  1:1438012/239‑1 (MQ=255)

                                                                                                                                                                                                                  cAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGc                                                                                                    >  2:667110/1‑162 (MQ=255)

                                                                                                                                                                                                                     gcggc‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCg                        >  1:164080/1‑235 (MQ=255)

                                                                                                                                                                                                                           atggCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTA‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCtctc                                                                                                                                <  1:912209/123‑1 (MQ=255)

                                                                                                                                                                                                                           aTTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTc               >  1:746515/1‑239 (MQ=255)

                                                                                                                                                                                                                             tGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCg              >  1:135201/1‑238 (MQ=255)

                                                                                                                                                                                                                              gcgggCGGACAAACGCATCACAAATACGGAAGGTG‑GCGGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGcc            <  1:1631172/236‑1 (MQ=255)

                                                                                                                                                                                                                                 ggCGAACAAACCCATC‑CCAAAACCGAAGGGGCGCGCC‑TTA‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGt          <  2:934948/238‑1 (MQ=255)

                                                                                                                                                                                                                                        aaaCGCATACCCAACCCCGAAGGTG‑GCACT‑TTT‑ACCAGCCACTACGGTGTCAAAGTTTTTGGCATCAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATc  <  1:697206/239‑1 (MQ=255)

TAGCGCATCATCCACCGATTTAAGCCCGCAGGCCATTGCCCGTACTGTACAGGCGGCGCTGGATATTGCCCGTTATACCTCGCCAGA‑TCCCTGTGCCGGCGTGGCAGACAA‑AGAGCTGCTGGCCTTTGACGCACCAGATCTCGACTTGTTCCACCCTGCGGAAGTTT‑CCCC‑GGATGAAGCCATTGAACTGGCGGCCCGC‑GCAGAACAGGCGGC‑ATTGCAGGCGGACAAACGCATCACCAATACCGAAGGTG‑GCAGC‑TTT‑AACAGCCACTACGGTGTCAAAGTTTTTGGCAACAGCCACGGCATGTTGCAGGGTTACTGCTCAACGCGTCATTCGCTCTCCAGCTGTGTAATTGCCGAAGAAAATGGCGATATGGAGCGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATC  >  NC_007779/4462869‑4463333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`