BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,796	C→G	22.5%	P31P (CCG→CCC)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,796	0	C	G	22.5%	187.2 / 17.8	109	P31P (CCG→CCC)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base C (42/38); new base G (2/22); total (47/62)
Fisher's exact test for biased strand distribution p-value = 9.95e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 109 total aligned reads displayed.

CCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954568‑955017

ccAGGTTATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  2:518830/239‑1 (MQ=255)

ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  1:1642584/239‑1 (MQ=255)

ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  1:513940/239‑1 (MQ=255)

ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  1:1380226/239‑1 (MQ=255)

ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  1:773048/239‑1 (MQ=255)

ccAGGCGATACAGCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTATAGAGAACGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAATGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGGAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  2:22912/239‑1 (MQ=255)

     tGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGtt                                                                                                                                                                                                                     <  1:1051538/236‑1 (MQ=255)

     tGATGCGCCCACTCGCCGTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGCCTTAAGCGGATGTTTCGtt                                                                                                                                                                                                                     <  1:1492099/236‑1 (MQ=255)

       aTGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCttt                                                                                                                                                                                                                >  1:1040382/1‑239 (MQ=255)

       aTGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCCGAGGTTTCGTTGCttt                                                                                                                                                                                                                >  1:18258/1‑239 (MQ=255)

       aTGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGAAAGAAAAGCTTAAGCGGATGTTTAGTTggctt                                                                                                                                                                                                                >  2:269053/1‑235 (MQ=255)

            cccACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGGTTT‑ATAGa                                                                                                                                                                                                          >  1:1346650/1‑239 (MQ=255)

                             caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CCCCCGCCTCTTCGGcc                                                                                                                                                                                        >  1:1167847/1‑239 (MQ=255)

                               acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TTGCC‑AGATAGAAAGACTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCCGCCa                                                                                                                                                                                       >  1:624739/1‑238 (MQ=255)

                               acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGAGGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGcc                                                                                                                                                                                        >  1:280634/1‑237 (MQ=255)

                                   tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       <  1:1332842/204‑1 (MQ=255)

                                   tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                       >  2:1332842/1‑204 (MQ=255)

                                        acGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAATCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCAt                                                                                                                                                                             >  1:871448/1‑239 (MQ=255)

                                           gTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTg                                                                                                                                                                           >  1:1147672/1‑238 (MQ=255)

                                           gTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCCCC‑AGATAGAAAGGCTTAAGGGGATGTTTCGTTGCTTT‑TTAGA‑CAACCGCCTCTTCCGGCCCTTTGGCCAttt                                                                                                                                                                           >  2:1553841/1‑237 (MQ=255)

                                            tGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTTGCCATTgca                                                                                                                                                                         >  1:1767693/1‑239 (MQ=255)

                                               aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      >  1:256626/1‑239 (MQ=255)

                                                agaaaaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTgcagca                                                                                                                                                                      <  1:372019/235‑1 (MQ=255)

                                                 gTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTTGCCATTGCAGCAgg                                                                                                                                                                    >  1:1347833/1‑239 (MQ=255)

                                                 gTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGAAAGAAAGACTTAAGAGGACGGTTCGTTGGCTT‑ATACA‑CACCCCCCCCTTTGGCTACTTTGGCCAATgcacacag                                                                                                                                                                    >  2:1254173/1‑234 (MQ=255)

                                                   aaaaagtccGGCCCCGCAGAAAACAAAAAGAATCGGCCCCAGAGAGAAGAAAATGCCGCC‑ACCCATTTTT‑GCCATGCGGAAGGGAATTGTGCCTGTGCCGGTGGTTGCTGTAATATAGAAGACAAATGCGATT‑GAGATGAAACCCCC‑GGCGGTAA‑TCCCC‑AGATAAAAAGTCTTAAGCGGTTGTTTCGTTGTTTT‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  1:941504/229‑1 (MQ=255)

                                                   aaaGAGTGCGCCCCCGCAGACAAAAAAAAAAACCAGCCCCGAGGAGAAGAAAGGGCCGCA‑ACCCAGTTTG‑GCAAGGCCGAAGGGTATGGGGCTGGGGCCGGTGGTTGTGGGAATAAAGAAGACGAAGGCGTTG‑GAGATAAAAACCCC‑GGGGGTAA‑TCACA‑AGAGAGAATGTTTAAAGGGGATGTTT‑GTTGGTTTGAAAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  2:1767693/237‑1 (MQ=255)

                                                   aaaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCAGGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGTTT‑GAGATAAAACCCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGGGGTTTTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  1:1493696/237‑1 (MQ=255)

                                                    aaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TTGGC‑AGAAAGAAAAGTTTAAGGGGATGTTTTGTTTCTTT‑AAAAA‑CACCCCCCTCCTCCGGCACTTTGGGCATTTCAGCCAGAAg                                                                                                                                                                 >  2:48035/1‑239 (MQ=255)

                                                    aaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAg                                                                                                                                                                 >  1:1590687/1‑239 (MQ=255)

                                                        gaccGGCTCCGCAGACAACACAAAGAATCACCCCCAGAGAGAAGAAAATGCCGCC‑ACCAAGTTTT‑GCCATGCCGAAGGGCTTTGTGCCTGTGCCAGTGGTTGCGGTGATATAGAAGACGAATGCGATT‑GAGATAAAAACCCC‑GGCGTTAA‑TCGCC‑AGATAGAAAGTCTAAAGCGGATTTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                              <  1:259862/235‑1 (MQ=255)

                                                        gaTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTTGCCATTGCAGCAGGAAGTaaa                                                                                                                                                             >  1:661769/1‑239 (MQ=255)

                                                              cTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCa                                                                                                                                                       >  1:1065842/1‑239 (MQ=255)

                                                              cTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCCCC‑AGATAGAAAGTCTTAAGCGGGAGTTTCGTTGGTTT‑ATAGA‑CACCAGCCTCTTCGGCCACTTTGGTCATTGCAGCAGGAAGTAAAAGATCa                                                                                                                                                       >  1:635283/1‑239 (MQ=255)

                                                                 cGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGACTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCaaaa                                                                                                                                                    >  1:1821224/1‑239 (MQ=255)

                                                                        aacaCAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGGTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGGAA‑GAGTC‑CCATAGGAAGTATTTAGTGGATGGTTAGTTGCCTT‑ATAGA‑GCACCGCATCTTCGGCCAACTTGGCCATTGAAGCAGGAATAAAAAGATCAACAGTGtttt                                                                                                                                             >  2:1866055/1‑237 (MQ=255)

                                                                               aGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAAGGCGATT‑GAGATAAAACCCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCttt                                                                                                                                      <  1:1340105/239‑1 (MQ=255)

                                                                                  aTCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGGTTCGTTGCTTT‑ATAAA‑CACCCGCCTCTTCCGCCACTTTGGCCATTGCAACAGGAAGGAAAAAATCAAAAGGGGTGTCAGGTTTaac                                                                                                                                   >  2:80615/1‑239 (MQ=255)

                                                                                   tCACCCCCAGAGAAAAGCAAATGCCCCC‑ACCCATTTTT‑CCCATGCCGAAGGGCATTGTCCCTGTGCCAGTGTTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAACCCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTTTTAAGGGGTTTTTTCGTTGTTTT‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcaca                                                                                                                                  <  1:1270718/239‑1 (MQ=255)

                                                                                   cccgCCCCAGGGAGAAGCAAAGGCCGCA‑AACCAGTTTT‑GCCAGGCCGAAGGGCGTTGGGCTTGGGCCGGGGGTGGCTGTGATATAGAAGACGAATGCGATT‑GAGAGGAAACCCCG‑GGGGTTAA‑CCGCC‑AGAAAGAAATTCTAAGGGGGTTTTTTCGTTTTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcaca                                                                                                                                  <  2:29189/236‑1 (MQ=255)

                                                                                   caccccccAGAGAGAGGC‑AATGCCCCCAAACCATTTTT‑GCGTGGCCGAGGGGATTTGTGCTGGGGCCAGTGTTGTGTTTGATATAAAAGAAGAATGCGATA‑GAAATGAAACCCCC‑GGCGGTAA‑TCCCC‑AGATAAAATTTGTTAAGCGGTTTTTTCTTTGCTTT‑AAAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAAGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcaca                                                                                                                                  <  2:365297/235‑1 (MQ=255)

                                                                                     aGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TTGCC‑AGATAGAAAGTCTTAAGCGGATGTTTTGGTGATTT‑ATAGA‑AACCCGCCTCTTCGGCCACTTTGGCCCTTGCGGTAGGTAGTAAAAGATCAAAAGGGTTGGGAGCttttccccc                                                                                                                                >  2:1305985/1‑233 (MQ=255)

                                                                                       ccccAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCCTCGGCCACTTTGGCCATTGTAGGAGGAAGTAAAAGAACAAAAGGGTTGTCAGCTTTCACACTaa                                                                                                                              >  2:949369/1‑239 (MQ=255)

                                                                                       ccccAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCCCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAGGGGTGTCAGCTTTCACACTa                                                                                                                               >  1:1253300/1‑238 (MQ=255)

                                                                                          ggagaggaacgaaaTGGCGGC‑AACCAGTTTTCGCCAGGAGGAGGGGTTTTGGGCTGGTGCCAGTGGTTTTTTTGAAATAGAGGAGGAAGGCGTTG‑AAGAAAAAACCCCC‑GGCGTAAA‑TCCCG‑AGAAAAAAGTTCTTAAGCGTTTTTTTTTTTGCTTT‑ATGAA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAct                                                                                                                            <  2:600787/228‑1 (MQ=255)

                                                                                           agagagAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTTAGCGGAGTTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTTCAGCCGGAAGTAAAAGATCAAAAGGGTTTGTAGCTTTCACactcactc                                                                                                                           >  2:890992/1‑238 (MQ=255)

                                                                                           agagagAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑ACGCC‑AGATAGAAAATCTTAAACGGGTGTTTTGTTGCTTT‑TTACA‑CACCCCCCCCTTCGGCCCCTTTGGCCATTTTCGCCGGGAGTAAAAAATCAAAAGGGGTGTCAGCTTTCACACAAAccc                                                                                                                           >  2:687674/1‑236 (MQ=255)

                                                                                            gagagaAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCCAAAGGGTTGTCCGCTTTCACACTAActc                                                                                                                           >  1:1637215/1‑237 (MQ=255)

                                                                                              gaagagaaaaTCCAGCC‑AACTTTTTTG‑GCCATGCAAAGGGGATTTGGCCCTGTGCGTGTGGTTGATGGAATAGAGAAAAGAAAGGATATA‑GAGATAAAAACCCG‑GGGGGTAA‑CCCAC‑AGAAAGAAATTGTTAAGGGGTTGTTTCGTTGCTTT‑AGACA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctct                                                                                                                        <  2:1928213/231‑1 (MQ=255)

                                                                                                 aaGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCttt                                                                                                                    >  1:1737534/1‑239 (MQ=255)

                                                                                                           cgcc‑AACCAGTTTT‑GCTATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTTA‑TCGCC‑AGATAGAAAGTCTTAAACGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCCTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGAACAAAAGGGGTTTCAGCCTTACCAATAACTCTCCCTTTTATAAATCga                                                                                                          >  2:874039/1‑238 (MQ=255)

                                                                                                                aaCCATTTTT‑GCCATGCCGAGGGGCATTGTGCCTGTGCCAGGGTTTGCTGTAATATAAAAGACAAATGCGATT‑GAGAAAAAAACCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGTATTTTTCTTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  1:1553841/239‑1 (MQ=255)

                                                                                                                  ccAGTTGT‑GCGAGGCGGAGGGGCGTTGGTCGTGGGCTAGTGTTTGTTGTAAAAAAAAAGACAAATGCGATT‑AAGATAAAACCCCC‑GGTGGTAT‑CCACA‑ATATAAAAAGTCTTAAGTTGGTTTTTTGTTGTTTA‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgacga                                                                                                    <  2:256626/239‑1 (MQ=255)

                                                                                                                           gcctggcggAAGGGCATTGGGCCGGTGCCAGTGGTGGTTTTGATATAGAGGACGAATGCGATT‑GAGATAAAACCCCC‑GGCGTTAA‑CCCCC‑AGAAAGAATGTTGTAGGGGGTTTTTTTGTTGTTTT‑ATAGC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            <  2:1846954/231‑1 (MQ=255)

                                                                                                                             ctggcggAAGGGCATGGTGCCTGTCCCAGTGTTTGTTTGGATATAGAAGACGAATGCGATG‑GAGATAAAAACCCC‑GGCGGTAC‑TCGCA‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGTTTA‑ATAGC‑CGCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1866055/233‑1 (MQ=255)

                                                                                                                             cacgCCGAGGGGCTTTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATG‑GAGATGAAAACCCG‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTAAGGCGGTTGTTTGGTTGTTTT‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1560315/236‑1 (MQ=255)

                                                                                                                             cATGCAGAAGGGTTTTGGGCCTGTGCGAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAACCACC‑GGCGGTAA‑CCGCC‑AGAAAGAAAGTCTTAGGCGGTTTTTTCGTTGTTTT‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1305985/239‑1 (MQ=255)

                                                                                                                                gCCGAGGGGCTTTGTGCCTGTGCCAGTGTTTGCTGTGATATAGAAGACAAATGCGATT‑GAGATAAAAACCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGTTGTTTCGTTGTTTT‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  1:1908623/239‑1 (MQ=255)

                                                                                                                                gCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACCCC‑GGCGGTAA‑TCGCA‑AGATAGAAAGTCTTAGGCGGTTTTTTTGTTGCTTT‑AGAGC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  1:1417530/239‑1 (MQ=255)

                                                                                                                                 ccGAGGGGGTTTGGGCCTGTGCGGGTGGTGGCGGTAATAAAGAAGACGAATGCGTTT‑GAGA‑GAAAACCCCGGGGGGTAA‑CCGCC‑AGATAGAAAGTCTAAAGGGGATTTTTCGTTGTTTA‑AAAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACaa                                                                                         <  1:1322894/236‑1 (MQ=255)

                                                                                                                                 ccGAAGGGCTTTGTGCTTGTGCCAGTGGTTGGTGTTATATAGAAGACGAATGCGATG‑GAGATGAAACCCCC‑GGGGGTAA‑TCGCC‑AGATAGAAAGTCTAAGGCGGTTGTTTGGTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  1:792552/238‑1 (MQ=255)

                                                                                                                                          tttGTGCCTGGGCGAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATAAAAACCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGTTTTTTCTTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGAt                                                                             <  1:1725802/238‑1 (MQ=255)

                                                                                                                                          tttGTGCCTGGGCCGGTGGTTGCTGTGATATAAAAGAAGAATGCGATT‑GAGATGAAACCCCC‑GGCGGTAA‑CCGCC‑AGATAGAAATTCTAAAGGGGTTGTTTGGTTGTTTT‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGa                                                                              <  1:890992/237‑1 (MQ=255)

                                                                                                                                            tgtgcctgtgccGGGGGTTGTGGTAATAGAGAAAAGGAATTAGATT‑GAGATGAAAACCCC‑GGGGTTAA‑TCGCC‑AGAAAGAAGTTCTTAGGGGGTTGTTTCGTTTCTTA‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           <  2:820035/239‑1 (MQ=255)

                                                                                                                                                 ttgtgccGGTGTTGGCTTGAATATAGAAG‑CGAAAGGGGTTGGAGATAAAACCCCC‑GGCGTTAA‑TCGCA‑AGATAGAAAGTCTTAAGCGGATGTTTTGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAt                                                                      <  1:1373867/238‑1 (MQ=255)

                                                                                                                                                 gtgggctaGTGGTTGCGGTGATATAGAGGAGAAATGCGATG‑GAGATAAAAACACC‑GGGGGTAA‑CAGCC‑AGATAGAAAGTCTTAAGCGGTTTTTTCTTTGTTTT‑AAAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  2:1395306/231‑1 (MQ=255)

                                                                                                                                                   gtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑CGATAGAAAATCTTAAGCGGATGTTTCGTTTCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAAGGTTGTCAGCTTTCACACTAACTACCTCTTTATTAAGTCGGCGACCAAGTACTAACAAAACATTATAAATGAGAAAttt                                                                    >  2:1468435/1‑238 (MQ=255)

                                                                                                                                                    tgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAAAAAAGCATTATAGATGAGAAATTGa                                                                   >  1:1094027/1‑239 (MQ=255)

                                                                                                                                                      caaGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAACCCCC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCTTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGat                                                                  <  1:1647175/236‑1 (MQ=255)

                                                                                                                                                       gagggttggtggtgATATAGAAGAAGAAGGCGGTG‑AAGATAAAAACACC‑GGCGTAAA‑TCGCC‑AGAAAGAATGTTTAAAGGTGATGTTTCGTTGTTTT‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:624739/227‑1 (MQ=255)

                                                                                                                                                       gaGTGGTTGGTGTGATATAGAAGAGGAATGAGATG‑GAAAGAAACACACC‑GGTGGAAA‑TCGCC‑AGAGAGAAAGTATTAGGCGGTTTTTTGGTTGTTTT‑AAAAA‑ACCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGatat                                                                <  2:1821224/238‑1 (MQ=255)

                                                                                                                                                       cggggtttGCTGTAATAAAGAAGACGAATGCGATG‑GAGAGAAAAACCCG‑GGCGGTAA‑CCGCA‑AGATAGAAAGTCTTAGGGGGTTGTTTCTTTGTTTA‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  1:464103/232‑1 (MQ=255)

                                                                                                                                                       cAGTGGTTGCTGTGATAAAGAAGAGGAATGCGATT‑GAAATAAAAACCCC‑GGCGTTAA‑CCGCC‑AAATAGAAATTTTTAAGGGGATTTTTGGTTGCTTT‑AAAGC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGatat                                                                <  1:1344161/239‑1 (MQ=255)

                                                                                                                                                       aggtgttggttgtaATAAAAAAGACAAGTGCGTTT‑GAGATGAAACCCCG‑GGCGTAAC‑CCGCC‑AGAAAGAAAGTCTAAAGGGGTTGTTTCGTTTTTTT‑AAAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  1:380150/228‑1 (MQ=255)

                                                                                                                                                            gtggCTGTGAAAAAGAAGAGAAATGCGATG‑GGAAAGAAAACACC‑GGGGTTAT‑CCGCC‑AAAAAAAAAGTTTAAAGGGTATGTTTGGTTCTTTA‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                            <  1:1721851/235‑1 (MQ=255)

                                                                                                                                                            gTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATc                                                           >  1:224504/1‑239 (MQ=255)

                                                                                                                                                               gCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGGC‑AGGTAGAAAGACTTAAGAGGATGGTTTCTTGATTT‑CTAGA‑CACCCGCCTCCTCGGCCACTTTGGCCAGTGCAGCCAGAAGTAAAATAACAAAAAGGTTGGTAGCTTTTACACTAATTCTCTCTTTATTAAATCAGGGACCAGAAAATAAAAAAACCTTATAAAAGAGAAAATAAtataaaactta                                                        >  2:1277523/1‑232 (MQ=255)

                                                                                                                                                               gCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑TTAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAATCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAACATtata                                                                                >  1:773381/1‑215 (MQ=255)

                                                                                                                                                                   tgATATAGAAGAGGAATGCGATT‑GAGATGAAAACCCC‑GGCGGTAA‑TCGCC‑AGAAAGAAAGTCTTAAGGGGTTGTTTCTTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGatat                                                                <  1:656619/227‑1 (MQ=255)

                                                                                                                                                                     atatAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCg                                                   >  1:322475/1‑238 (MQ=255)

                                                                                                                                                                      tataGAAGAGAAAGGCTTTT‑GAGATAAAAACCCC‑GGGGGTAA‑TCGCC‑AGATAGAAAGTCTAAAGGGGATGTTTCGTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                  <  1:1318638/238‑1 (MQ=255)

                                                                                                                                                                      tataGAAGACGAATGCGTTG‑GAGATAAAAACCCC‑GGCGGTAA‑CCGCA‑AGATAGAAAGTCTTAGGGGGATTTTTCGTTGTTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1684647/239‑1 (MQ=255)

                                                                                                                                                                      taaagaagaCAAATGCGATT‑GAGAAGAAAACCCG‑GGGGGTAA‑TCGCC‑AGATAGAAAGTATTAAGGGGGTGTTTCGTTGTTTT‑AAAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1039381/236‑1 (MQ=255)

                                                                                                                                                                      taaaaaggaaaaaTGCGATG‑GAGAT‑AAAACCCC‑CGCGGGAACCCCCC‑AGAAAAAAATCTTTAAGCGGGTTTTTCTTTGTTTT‑AGAGC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:812845/228‑1 (MQ=255)

                                                                                                                                                                      aataAAAGACAAAGGCATTG‑GAGAT‑AAAACCCC‑CGGGGTAA‑ACCCCAAGATAAAAGGTCTTAAGCGGTGTTTTCTTTGTTTT‑ATAGA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:687674/238‑1 (MQ=255)

                                                                                                                                                                           aagaCGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAGGGATGTCAGCTTTCACACTAACTCTCTCTTTATTAAGACGGCGAAGAGATACTAACAAAACATTATAGATGAGAAAATGATATAAAACATATCTCGCCTGGCt                                            >  1:1651662/1‑239 (MQ=255)

                                                                                                                                                                             gaCGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGGTGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCCGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGAAGAGATACTTACAAAAAATTATAGATGAGAAATTGATATAGGTCATAtc                                                      >  2:656619/1‑227 (MQ=255)

                                                                                                                                                                               cGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       >  1:1607314/1‑192 (MQ=255)

                                                                                                                                                                               cGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTtata                                        >  1:1335843/1‑239 (MQ=255)

                                                                                                                                                                               cGAAGGCGATG‑GAAATGAAAACCCC‑GGCGTAAA‑CCGCA‑AGATAAAAAGTCTTAAGCGGTTGTTTCTTTGTTTA‑ATAGC‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          <  1:79014/237‑1 (MQ=255)

                                                                                                                                                                                       aTT‑GAGATAAAACCCCC‑GGCGTTAA‑CCGCA‑AGAAAAAAAGTCTAAGGCGGTTTTTTTGTTGTTTT‑AAAAA‑CCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGt                                 <  1:1277523/238‑1 (MQ=255)

                                                                                                                                                                                              aTGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCt                                                                                                                                                                                                                  <  2:19566/57‑1 (MQ=255)

                                                                                                                                                                                              aTGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCt                                                                                                                                                                                                                  >  1:19566/1‑57 (MQ=255)

                                                                                                                                                                                               taaaaaCACC‑GGCGGTAA‑TCGCC‑AGATAGAAATTCTTAAGCGGATGTTTGTTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  1:1016565/237‑1 (MQ=255)

                                                                                                                                                                                                                   tccccaagaaagaaAGTCTTAAGTGGATGTTTCTTTTCTTT‑ATAAC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:1742078/233‑1 (MQ=255)

                                                                                                                                                                                                                     cgcatgATAAAAAGTTTTACGCGGATGTTTCGTTGTTAT‑AAAAC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACg      <  1:1226601/234‑1 (MQ=255)

                                                                                                                                                                                                                         atatggagtgTCTGAAGCGCATTTTTGGTTG‑TTT‑ATAAACCCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  2:1637215/230‑1 (MQ=255)

                                                                                                                                                                                                                         ataTAGAAAGTCATAAGGGGTTGTTTGTTTGCTTT‑ATAGC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:1678534/237‑1 (MQ=255)

                                                                                                                                                                                                                         aGATAGAAAGTCTAAAGGGGATTTTTCGTTGTTTT‑AAAAC‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:949369/239‑1 (MQ=255)

                                                                                                                                                                                                                            gaGAAAGTCTTAGGCGGGTTTTTTTTTGTTTT‑AGAGC‑CCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:464702/235‑1 (MQ=255)

CCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCC‑AACCAGTTTT‑GCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATT‑GAGATGAAAACACC‑GGCGGTAA‑TCGCC‑AGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTT‑ATAGA‑CACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954568‑955017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`