| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_007779 | 75,079 | C→T | 20.1% | L134L (CTG→CTA) | thiB ← | thiamine ABC transporter substrate binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 75,079 | 0 | C | T | 20.1% | 121.0 / 8.8 | 53 | L134L (CTG→CTA) | thiB | thiamine ABC transporter substrate binding subunit |
| Reads supporting (aligned to +/- strand): ref base C (2/40); new base T (9/2); total (11/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCAGATCGCTTTCACCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCC > NC_007779/74845‑75303 | tcccagcgcGCTTTC‑CCTTTTAAAACACGGCCCTTAGGTTCCGCTCCACGTTTTGGGGCCCGGGACCGTTTTCTTCCCCTGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATGGACTTTTTGCTTCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGTTCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGt < 2:1293103/230‑1 (MQ=255) tCGCTTTCCCCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCt < 1:839937/238‑1 (MQ=255) tCGCTTTCCCCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc < 1:957026/237‑1 (MQ=255) cGCTTTCACCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTttttt > 1:746323/1‑238 (MQ=255) cTTTCACCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTttttttttttt > 1:265139/1‑236 (MQ=255) aaacgggcgtgaGGCTGCCCTCCGGCTTGTGGTGCCCGTACCCTTTTTTTTCCCCAGTTTCTCCCAGCCTTGGGGGGCTCCTTCCCCATGGACTTTTTCCACCCATACCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGa < 2:993518/228‑1 (MQ=255) ccgggCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑CCCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAg < 1:718323/236‑1 (MQ=255) acgggCCGTGGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCATTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGCTTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAg < 1:1943996/236‑1 (MQ=255) gCCGTAGGCTTCGTCCCAGCTTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGTCTTTTTGCATCCATAGCAACAG‑CCCCACCCCCGGTGTACT‑GGTGCGCGTATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑a < 1:1083732/220‑1 (MQ=255) gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTTGTTTGTTTTTTTT‑ATTAACAAAAGCgtgggag > 1:1642365/1‑232 (MQ=255) gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTGTT‑TTAAAACAAGAGGAAGTAg > 1:222667/1‑238 (MQ=255) ctgtagttttCGCCCAAGCTTTTGGTCCCCGGGCCCGTTTTTTTCCCCCGTTTCGGACAGGCTTGTGGAGCTTACTCGCTATAGCTTTTTTGCA‑CCCTAACAACCGCCCCCCGCCCGGGTGTCCT‑GGTGCGGGGTTCTTGATAAT‑TCCCCCGCCAGTTTTGATCGCTCTCACCCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGc < 2:1031467/230‑1 (MQ=255) ccagcgctttCGCTCCTGCTTGGGGTGCCTGTTACCTTTTTCTTCGCTTGTTCCCCCCGTTTTTGCGGCGCGTCATGGGCTTGGTTTTTTTGCTTCCTTAACACCAC‑CCCCGGCCCGGGTGTCCT‑GTTGCGGGGATCCTGAGAAT‑TCCCCCGCCAGTTTTGATCGCTCTCATCCTGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTCGTC‑ATAAACTAAGGCGAAGTAGc < 2:278589/230‑1 (MQ=255) ccGTAGGCTTCGCTCCAGCCTTGGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGt < 1:1367091/235‑1 (MQ=255) gtatgtctCGCCCCAGGCTTTGGTGGCCGTGACCGTTTTTCTCGCCTGTTTCCGCCAGGGTTAGGGGGGGTCAGTGGCATTGGCTTTTTGCATCCATAGCAACCGCCCCCACCCCCGGTGTCCT‑GGGGCGCGGTCCTTGAAAAT‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCa < 2:1860579/232‑1 (MQ=255) cTTCGCCCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGATTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGGGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCa < 1:1214024/239‑1 (MQ=255) tCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCaaa < 1:1217385/239‑1 (MQ=255) tCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTGTTTGTTTTTTTT‑ATTAAACAAAGCGAAGAAGCCATAATCaaa > 1:1955543/1‑239 (MQ=255) tttGGTGACCGTGCCCGTTTTCTTCGCAAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑CCCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAg < 1:329836/239‑1 (MQ=255) gtgacggtgaccGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgtgt < 1:685854/238‑1 (MQ=255) cgtgaccgtTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTGTCTTTAAAGAAAGGGGAAG‑AACCAAAATCAAAAGATACGaaaagatcgttat > 1:689570/1‑229 (MQ=255) accgtTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTTTTTGTTTTTTTT‑TTTAACGAAAGCGAAGACACCACTATCAAACCGTGCGACAGGGTCATTTTTcct > 1:1790564/1‑238 (MQ=255) ttcttcGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTTGTTTGTTTTTTTT‑ATCATCAAAAGCGCAGAGGTGCTACACAAACAGTGAGAAAGAGTCATTATTTCTgcccc > 2:470818/1‑236 (MQ=255) tgcGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑CCCCAGCCCCGGTGTATT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg < 1:1803231/237‑1 (MQ=255) tcGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGTTTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTCCT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa < 1:429711/239‑1 (MQ=255) cGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTC‑TTTAAACAAAGCGGAGAAGCCCTCATAAAAAAGGACGGAAAAGTTATTATTTCAACCCCCCGGa > 1:1753079/1‑238 (MQ=255) ttGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCt < 1:1940486/238‑1 (MQ=255) ggcgttatgcgcATAGTCTTTTTGCACCCA‑AGCAACCA‑ACCCCACCCCGGGGTATTGGGGGCCCGGACGCT‑ATAAATTCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGATGGCAAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCcaca < 2:1753079/227‑1 (MQ=255) gTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑CCCCAGCCCGGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGc < 1:1156341/239‑1 (MQ=255) tCATCGCCATAGATTTTTTGCATCCATACCAACAG‑ACCCAGCCCGGTTGTACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGc < 1:55155/238‑1 (MQ=255) cATAGCCTTTTTGCATCCATAGCAACAG‑CCCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCa < 1:1872435/239‑1 (MQ=255) tCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTT‑TTAAAAAAAAGCGAAGTAGACATAATAAAACGGTACGAAAGTGGCATTATTTTCACCGCCGGGGAGGTTAAAGGGATCCGCTGCCACACCCCCTTTTGCAAACAATACCGGtttt > 1:1751595/1‑239 (MQ=255) aaCAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTggcg < 1:883545/237‑1 (MQ=255) aCCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTT‑ATAAAAGAAAGCGGAGGAGCCATAATCAAACAGGAAGAAAGAGTCATTATTTCAGCCGCCGGGGACGGTAAAGGCATCCCCTGCCACACCGCTTTTTGCAAACAAA‑CCGGGTTTTCTTGGGGCGGCtt > 1:70203/1‑238 (MQ=255) ccAGCCCGGTTTTCCG‑GGTGCCCGACTCCTGAAAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAAc < 1:1234642/239‑1 (MQ=255) ccAGCCCGGGTGTCCG‑GGGGCGCGGATCCTGAAAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAAc < 1:1759988/239‑1 (MQ=255) ctgCCCCGGTGTAGC‑GGTGCGGGAGTCTTGAAAAA‑TCCCCCGCCAGTTTTGATCGCTCACAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGGC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACa < 1:260724/237‑1 (MQ=255) ccGTTGTCCT‑GTTGCGCGGACCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGgttg < 1:458034/239‑1 (MQ=255) gtgtACT‑GGTGCGCGGATCCTGATAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCcaca < 1:1892702/188‑1 (MQ=255) aCT‑GGTGCGCGGATCCTAAAAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc < 1:470818/238‑1 (MQ=255) gcgGATCCTGATAAA‑CCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGc < 1:295459/239‑1 (MQ=255) ggATCCTGAAAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGca < 1:1411492/238‑1 (MQ=255) ctaaTAAA‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt < 1:315882/236‑1 (MQ=255) a‑TCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGACAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCttt < 1:1527692/239‑1 (MQ=255) ccccccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGACAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTa < 1:1527704/236‑1 (MQ=255) tttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCt < 1:679670/239‑1 (MQ=255) tGATCGTTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTc < 1:1866604/239‑1 (MQ=255) tGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTc < 1:844147/239‑1 (MQ=255) tCGTTCTCAACCATTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCAt < 2:987362/239‑1 (MQ=255) tcAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGtttt < 1:838465/223‑1 (MQ=255) cacccggTTCTTTCAGGCTTTGGGGGGGTTTTTTTAGTTGGTTCGTGTC‑ATAAACGAGGGCAGAGTGGCCATAATCAAACGGTACAAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGa < 2:222667/233‑1 (MQ=255) gtttggtgGCGGTTTTTTCAGTTTTTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGa < 2:1225153/232‑1 (MQ=255) tgtgGCGGGTTTTTTAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTAGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGcc < 1:1386173/239‑1 (MQ=255) | TACCAGATCGCTTTCACCTTTTAAAA‑ACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAG‑ACCCAGCCCCGGTGTACT‑GGTGCGCGGATCCTGATAAA‑TCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTC‑ATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGT‑CCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCC > NC_007779/74845‑75303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |