Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,371,2710A.93.7% 247.1 / 11.1 63pseudogene (183/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base . (55/4);  total (55/8)
Fisher's exact test for biased strand distribution p-value = 1.18e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0077794,371,2720A.93.7% 249.1 / 9.1 63pseudogene (182/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base . (55/4);  total (55/8)
Fisher's exact test for biased strand distribution p-value = 1.18e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAA  >  NC_007779/4371033‑4371503
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                       
gCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa                                                                                                                                                                                                                                          <  2:418845/239‑1 (MQ=255)
     cTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCCGCAGCTTTTCTGTCTGCTGAAAC‑AGATAGTCCCAACCGCCAGCAACCTGCATGCCCGaaaaag                                                                                                                                                                                                                                     >  2:1872320/1‑238 (MQ=255)
        cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                   >  1:453226/1‑233 (MQ=255)
          cgGATACCACTGCAGTAGACAGCGGACGGCAAGGATT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTTCGAGGACCGTGATGAATATCGGGTTACGGCCCAGCAGCTTTTCTGTCTGATGCACC‑AGATAGTCCAGACCGCCAGAAACCTGCATGTCAGtaatagcggc                                                                                                                                                                                                                                 >  2:1280467/1‑231 (MQ=255)
                  aCTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                                                                                                                                                                                                       >  2:2032671/1‑238 (MQ=255)
                           gACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                                                                                                                                                                                             >  1:1247203/1‑239 (MQ=255)
                           gACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                                                                                                                                                                                             >  1:106417/1‑239 (MQ=255)
                              cgggggagggaaGGTTTAAACCCTTTTTTCCTTCGCAACTTCAGGAATAACTGGAAGTGTCGCCAGAGAGATGTGGCAAGACCCAGAAAAGATAGTCAGAAAATGGGTCACGATGGGTGCGGGGTTGGTGATTTATTTGGGTTTCGGGCGCAGCATTTTTTTTGTCTAATGACCA‑AGATAGCCCAGCCCGCCAGCACCCTGAATGGAAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                                                                                                                                                                                             <  1:277758/227‑1 (MQ=255)
                              aGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGCTGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATACTGga                                                                                                                                                                                                          >  1:2106595/1‑239 (MQ=255)
                              aGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                          >  1:370589/1‑239 (MQ=255)
                              aGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                          >  1:859237/1‑239 (MQ=255)
                                cGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                        >  1:2162414/1‑239 (MQ=255)
                                cGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                        >  1:444776/1‑239 (MQ=255)
                                cGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCACCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATCGCGGCGATAACCGCCCTGACAATGgaga                                                                                                                                                                                                        >  2:2026290/1‑239 (MQ=255)
                                cGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCCCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGAAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCAAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAACGACATGATAATGgcga                                                                                                                                                                                                        >  2:1940800/1‑239 (MQ=255)
                                       cAAGGTTT‑ACCGCTTTGTTCCTCCGCACCTACAGCAAAAACGGGGAGGGTCGCAAGAGAGATGTTGCAAGTACAAGAAAAGATAGTGAGAAAATAGGTCACGTTGGGGGGGGGGATCGGGTTTTTTTTCGGTTTCCGGCGCAGCATTTTTTCTGTCTAATAACCA‑AGATAGCCAAGACCGCCAGCACCCTGCATGGAAG‑‑AAATAGCGGCAATAACCGCCATGATAATGGAGATGACAt                                                                                                                                                                                                  <  1:656069/238‑1 (MQ=255)
                                                       tGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAcc                                                                                                                                                                                  >  1:2087123/1‑239 (MQ=255)
                                                       tGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAcc                                                                                                                                                                                  >  1:12322/1‑239 (MQ=255)
                                                            cTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGACAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt                                                                                                                                                                             >  1:1853032/1‑239 (MQ=255)
                                                              tCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTa                                                                                                                                                                           >  1:270280/1‑239 (MQ=255)
                                                               cGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTa                                                                                                                                                                           >  1:343030/1‑238 (MQ=255)
                                                                         gCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGc                                                                                                                                                                >  1:1117866/1‑239 (MQ=255)
                                                                              aaCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCaa                                                                                                                                                           >  1:104564/1‑239 (MQ=255)
                                                                                         tCGCCAGGGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGAGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCCTGATAATGGAGATGAAATGGAAAGGGATGTGACCGGGTTTAAGGCTATTAGGGGGAAGAACCAGCag                                                                                                                                                 >  2:84529/1‑237 (MQ=255)
                                                                                         tCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTAACGAACGGTGCGAGGAACGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAc                                                                                                                                                 >  1:605559/1‑238 (MQ=255)
                                                                                                  agaTGTTGCCAGTCCCAGAAAAGATGGTAAGAAAATAGGTCACGATGGGTGGGAGGATGGTGATGTATTTGGGGTTACGGCGCAGCAGTTTTTCTGTCTGATAAACC‑AGATAGCCAAGCCCGCCAGCACCCTGCAGGGC‑‑AAAAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                          <  1:272095/172‑1 (MQ=255)
                                                                                                        tGCCAGTACAAGAAAGAATAGTCAGAAAATAGGTCACGATCGGTGCGAGAATGGTGATTTTTTGGGGGTTCCGGCCCAGCATTTTTCCTGTCGGA‑GAACCAAGATCCCCCAGCCCCCCAGCAACCGGCAGGGC‑‑AAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCa                                                                                                                                 <  1:1307908/239‑1 (MQ=255)
                                                                                                            aGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                                                                                                                                                                                                >  2:272095/1‑172 (MQ=255)
                                                                                                              tACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCa                                                                                                                           >  1:893702/1‑239 (MQ=255)
                                                                                                                   gCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCa                                                                                                                                                  >  1:565141/1‑211 (MQ=255)
                                                                                                                   gCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATa                                                                                                                        >  1:1564847/1‑237 (MQ=255)
                                                                                                                   gCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATa                                                                                                                        >  1:1877807/1‑237 (MQ=255)
                                                                                                                    cAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCt                                                                                                                     >  1:1196708/1‑239 (MQ=255)
                                                                                                                    cAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGGTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATAcat                                                                                                                     >  1:1997392/1‑237 (MQ=255)
                                                                                                                      aaGATAGTCAGAAAATGGGTCCCGTTGGGGGCGGGGTTTGTGATGTTTTTGGGTTCCCGGCGCACCACTTTTTTTGTCTAAGGAACA‑AGATAGCCCAGCCCGCCAGCAACCGGCAGGGC‑‑AAAAATGGCGGCAATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTAt                                                                                                                   <  1:178168/239‑1 (MQ=255)
                                                                                                                        gATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAccc                                                                                                                                               >  1:1170355/1‑209 (MQ=255)
                                                                                                                                       ggTCACGATCGGTGCGCGGATCGTGATGTATTCCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCAAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGCTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCCCCCGCAAAAACACTACCTATTCCCCCCAATCTGgcg                                                                                                   >  1:1861683/1‑238 (MQ=255)
                                                                                                                                          caggATCGGTGCGAGGTGGGGTTTGTATTGGGGGTCCCGGCGCAGCGGTTTTTCGGTATGA‑GAACCAAGATATCCCAGCCCACAAGAAACCGGCAGGGAAG‑‑AAATGGCGGCAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCa                                                                                               <  1:956965/236‑1 (MQ=255)
                                                                                                                                           aCGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCaa                                                                                              >  1:1882211/1‑239 (MQ=255)
                                                                                                                                                  gTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATc                                                                                       >  1:423464/1‑239 (MQ=255)
                                                                                                                                                             gTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCaaa                                                                              >  1:1027305/1‑237 (MQ=255)
                                                                                                                                                             gTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCaaa                                                                              >  1:128266/1‑237 (MQ=255)
                                                                                                                                                                  gTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGCTCGCCATCAAAACAatga                                                                       >  2:2040789/1‑239 (MQ=255)
                                                                                                                                                                    aTTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTatgatg                                                                     >  1:1197807/1‑239 (MQ=255)
                                                                                                                                                                     tttCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTAtgatga                                                                    >  2:1085561/1‑239 (MQ=255)
                                                                                                                                                                        cGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGt                                                                  >  1:1886731/1‑238 (MQ=255)
                                                                                                                                                                           gTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCt                                                               >  1:1725040/1‑238 (MQ=255)
                                                                                                                                                                           gTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCt                                                               >  1:887558/1‑238 (MQ=255)
                                                                                                                                                                             tACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATc                                                                                                        >  1:1286923/1‑195 (MQ=255)
                                                                                                                                                                              aCGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                            >  1:2171956/1‑238 (MQ=255)
                                                                                                                                                                               cGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTg                                                                                                                                   >  1:1951207/1‑166 (MQ=255)
                                                                                                                                                                                     agcagcTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCat                                                    >  2:895018/1‑239 (MQ=255)
                                                                                                                                                                                       cagcTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                            >  1:651722/1‑229 (MQ=255)
                                                                                                                                                                                                 gtctgATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGccttcct                                        >  1:204268/1‑239 (MQ=255)
                                                                                                                                                                                                 gtctgATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACAGCCATGATAATGGAGATGACATCGAACGGGATGTTAACGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCAAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGccttcct                                        >  1:2023834/1‑239 (MQ=255)
                                                                                                                                                                                                    tgatgaACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCCGCAAAACTatg                                                                        >  1:977469/1‑204 (MQ=255)
                                                                                                                                                                                                                ataccccACACCCCCAGCAACCGGCA‑GG‑AAAAAAAGAGCGGCGAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAg                          <  1:340107/234‑1 (MQ=255)
                                                                                                                                                                                                                 aaGTCCAGCCCGCAAGCAGCCTGTATCGCAG‑‑AAAGAGCGGAGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGt                         <  1:1737663/238‑1 (MQ=255)
                                                                                                                                                                                                                    tCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGa                      >  2:1045869/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAg                                               >  1:1486181/1‑212 (MQ=255)
                                                                                                                                                                                                                      cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGc                                              >  1:1294976/1‑213 (MQ=255)
                                                                                                                                                                                                                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                            >  1:737028/1‑196 (MQ=255)
                                                                                                                                                                                                                         aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTa                 >  2:1410484/1‑239 (MQ=255)
                                                                                                                                                                                                                              cAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTg            >  1:635724/1‑239 (MQ=255)
                                                                                                                                                                                                                                   aCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGc                                              >  1:28930/1‑200 (MQ=255)
                                                                                                                                                                                                                                     cTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGAGGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCCAGAAGATCGCCAGCAAAACTATGATGAGTTCTCCAACAAGCATATTAGCCTTCCTTGTTTTTTAACACGCTGATCTTATATTGTTAtttt     >  2:1175146/1‑239 (MQ=255)
                                                                                                                                                                                                                                        cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTaa  >  1:1733018/1‑239 (MQ=255)
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                       
GCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTT‑AACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAA  >  NC_007779/4371033‑4371503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑