BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	304,460	A→C	10.1%	F528V (TTT→GTT)	ecpD ←	fimbrial adhesin EcpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	304,460	0	A	C	10.1%	640.5 / 5.8	213	F528V (TTT→GTT)	ecpD	fimbrial adhesin EcpD
Reads supporting (aligned to +/- strand): ref base A (117/68); new base C (2/26); total (119/94)
Fisher's exact test for biased strand distribution p-value = 9.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGTACTT  >  NC_007779/304224‑304687

ccggggttttaaaTATCACTGATGGAAATACGGTTTATCCGTGCGCTTTTGTTGTTATCCACTACACGTTGGCTGACAAAGTCGTTCTC‑GGACGGAGGCGAAAATGTCATATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCG‑CAAAcc                                                                                                                                                                                                                                   <  2:280678/227‑1 (MQ=255)

cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACTGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGCGCCGCCATCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCATGCACATGACTCTCTCCTGAAGCGCTGA‑CTTCCCCAAAc                                                                                                                                                                                                                                    >  2:1856720/1‑238 (MQ=255)

cccGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAAcc                                                                                                                                                                                                                                   >  1:2082812/1‑239 (MQ=255)

     gCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCg                                                                                                                                                                                                                              >  1:576678/1‑239 (MQ=255)

       tgtCAATAGCCTGGAGGGCATTCCGGTATTTCCGTGCGTTTTTGTTGTTATGCACTCCCCGTGTGCTGAAACGGTCAGTCGC‑GGAGGGCGGCGAAAATGTCAGAGTGCCGACCGAAATCGGCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCGGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATAAATCTCTCCGGAGGCGCTGA‑CTTCGCAAAACCAGCCGtt                                                                                                                                                                                                                            <  2:882139/237‑1 (MQ=255)

       tATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGtt                                                                                                                                                                                                                            >  1:2012230/1‑239 (MQ=255)

            attgCACTGATGGCAAACCTGTATATCCGTGCGCTTTTGTGGTATTACACTACCCGTTTGCTGCCAAAGTCAG‑CTCGGGACGGAGGCGAAAAGGTCCGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTATTTAATGTTACGCCCCGTCGCCTGAACATGATTCTCTCCTGAAGCGCTAA‑CTTCGCCAACCCAGCCGTTatcat                                                                                                                                                                                                                       <  2:59423/236‑1 (MQ=255)

            aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAACCCAGCCGTTatc                                                                                                                                                                                                                         >  2:760652/1‑237 (MQ=255)

            aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTatcat                                                                                                                                                                                                                       <  1:1908858/239‑1 (MQ=255)

            aTAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTatcat                                                                                                                                                                                                                       <  1:1586764/239‑1 (MQ=255)

                   tGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGt                                                                                                                                                                                                                >  2:1886974/1‑239 (MQ=255)

                     aTGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTAc                                                                                                                                                                                                              >  1:207256/1‑239 (MQ=255)

                       ggCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGc                                                                                                                                                                                                            >  1:769553/1‑239 (MQ=255)

                           aTACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCg                                                                                                                                                                                                        >  1:1916648/1‑239 (MQ=255)

                            tACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGa                                                                                                                                                                                                       >  1:1262283/1‑239 (MQ=255)

                                   ttcccGTGCGTTTTTTTGTTTATTCACTACAGGTTGGCGGACAAAGTCAGTCTG‑GGCCGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCCCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCg                                                                                                                                                                                                <  2:1385099/236‑1 (MQ=255)

                                    atCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCg                                                                                                                                                                                                                              <  2:619656/208‑1 (MQ=255)

                                    atCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGt                                                                                                                                                                                               >  1:1982894/1‑239 (MQ=255)

                                         tGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCCGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTa                                                                                                                                                                                                                    >  1:772781/1‑213 (MQ=255)

                                         tGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATcc                                                                                                                                                                                          >  1:705208/1‑239 (MQ=255)

                                         tGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATcc                                                                                                                                                                                          >  1:1385099/1‑239 (MQ=255)

                                         tGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATcc                                                                                                                                                                                          >  1:1308715/1‑239 (MQ=255)

                                         tGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATcc                                                                                                                                                                                          >  2:509402/1‑239 (MQ=255)

                                         tGCGCTTTTGGTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGAAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGGGCCGCCAGCATACAGACCGCTGTCAGCAGGGGCTTAGTTAATGTTACGCCCCTTCGCCTGAAAATGAATCTCGCCTGAAGCGCTGA‑CTTCGCCAAACCATCCGTCATCGTCTACCGGCCGCAGCGACACGGCGCTATc                                                                                                                                                                                           >  2:1803867/1‑238 (MQ=255)

                                           cgcTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCAt                                                                                                                                                                                        >  1:375892/1‑239 (MQ=255)

                                           cgcTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCAt                                                                                                                                                                                        >  1:169780/1‑239 (MQ=255)

                                           cgcTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCAt                                                                                                                                                                                        >  1:1224151/1‑239 (MQ=255)

                                                        aTTCACTCCAAGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGAGCCGCCAGCATACAGACCGCTGCCAGCAGGGCCTTAGTCAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGTTGC‑CTTCGCCAACCCAGCCGTTATCATCTACCGCACGCAGCGAAAAGGCGTTATCCATTGGACTCGAACAt                                                                                                                                                                           >  2:2011740/1‑239 (MQ=255)

                                                        aTTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTa                                                                                                                                                                                                               >  2:921788/1‑203 (MQ=255)

                                                        aTTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAAt                                                                                                                                                                           >  2:306628/1‑239 (MQ=255)

                                                              tacccttttGGTAACAAAGCCAGTCGC‑GGCCGGCAGCGAAAATGTCAGATTGCCGCCCGAAATCGCTTGTGTCAAGGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCAAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACt                                                                                                                                                                     <  2:705208/233‑1 (MQ=255)

                                                              tACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGCCCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACt                                                                                                                                                                     <  1:1518893/239‑1 (MQ=255)

                                                              tACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACt                                                                                                                                                                     <  1:1569086/239‑1 (MQ=255)

                                                               ccccGTTTGCTGAAAAATTCGTCCTC‑GGGCGGCCGCGAAAATGTCATATTGCCGCCCGAAATCGCTTGTGTCAAAGGTCCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTATTGTTCCGCCCCGTCGCCTGAACAGGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACtg                                                                                                                                                                    <  2:580526/236‑1 (MQ=255)

                                                               accggTTTGCGCACAAGTTCATTCCC‑GGACGGCAGCGAAAATGTCTATTGGCGGACCAAATTCGCCTGGGTAAAGGGGCCCGCCAGCATACAGACCGCTGTCGGCGGGGCCTTAGTTAATGTTACGCCACGTCCCCTGAAAATGATTTCCTCGTGAAGCGTTAA‑TTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACtg                                                                                                                                                                    <  2:1224151/235‑1 (MQ=255)

                                                                cacGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACtgt                                                                                                                                                                   >  1:1163594/1‑239 (MQ=255)

                                                                cacGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACtgt                                                                                                                                                                   >  1:1398824/1‑239 (MQ=255)

                                                                  cGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTgg                                                                                                                                                                 <  1:614828/239‑1 (MQ=255)

                                                                   gTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTgg                                                                                                                                                                 >  1:303092/1‑238 (MQ=255)

                                                                   gTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTgg                                                                                                                                                                 >  1:1336150/1‑238 (MQ=255)

                                                                   gTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGt                                                                                                                                                                <  1:1846126/239‑1 (MQ=255)

                                                                      tGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTatc                                                                                                                                                                                                                         <  1:2051378/179‑1 (MQ=255)

                                                                        cTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTa                                                                                                                                                            >  2:1140901/1‑238 (MQ=255)

                                                                            cAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCAt                                                                                                                                                       <  1:1088528/239‑1 (MQ=255)

                                                                                 tcggtcCC‑GGAGGGGAGCAAAAATGTCAGAGGCCCGCCGGAAATCGGCTGTGTCAAAGGTGCCGCCAGCATCCAGACCGCTGTCAGCAGGGCCTTAGTGATTGTTACGCAACGTCGCCTGAACATGAATCTCTCCTGAAGGGCTGA‑CTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGc                                                                                                                                                   <  2:1830091/235‑1 (MQ=255)

                                                                                      ctc‑GGACGGCAGCGAAAATGTGAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGCCCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCGGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACt                                                                                                                                             <  1:1071151/239‑1 (MQ=255)

                                                                                           gACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCg                                                                                                                                          >  1:926949/1‑238 (MQ=255)

                                                                                             cGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGcc                                                                                                                                        <  1:180799/238‑1 (MQ=255)

                                                                                                cAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCtt                                                                                                                                                             >  1:2107673/1‑214 (MQ=255)

                                                                                                            cAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCa                                                                                                                         <  1:894731/238‑1 (MQ=255)

                                                                                                                ttGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGgtg                                                                                                                    <  1:335032/239‑1 (MQ=255)

                                                                                                                ttGCCGACCGAAATCCCCTGGGTCAGAGGTGCCGCCCGCATAAAGACGGCTGTCAGCAGGGCCTTAGTTAATGTTCCGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACg                                                                                                                       <  2:1550780/236‑1 (MQ=255)

                                                                                                                 tggcgaccaaAACCGCCGGTGTAAAGGGTCCCCCCAGCATCCAGACCGCTGTAAGAAGGGCGTTGGTTAAGGTTACCCCGCGCCCCCTGACCAGGATTCCCTCCGAAAGCGGGGA‑CTTCCCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGgtg                                                                                                                    <  2:1260891/235‑1 (MQ=255)

                                                                                                                  gCCGACGGAAATCGCCGGTGTAAAAGGTGCCGCGAGCATAAAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCTTGAACATGAATCTCTCCTGAAGAGCTGA‑ATTCGACAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCAAGAGATATCAGTCCACGGtgtg                                                                                                                  <  1:1856720/239‑1 (MQ=255)

                                                                                                                  accgaccgaATTCGCCTGTGTAAAGGGTGCCGCCAGCATCCAGACGGCTGGCGGCAGGGCTTTAGTGAATGTTACGCCCCGCCGCCTGAACAGAAACCTCTCCGAAACCGATGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGtgtg                                                                                                                  <  2:1699800/238‑1 (MQ=255)

                                                                                                                     gaccgaAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTc                                                                                                               >  1:1714403/1‑239 (MQ=255)

                                                                                                                           aaTCCCCTGTGTAAAGGTTGCGTCCAGCATACAGACCGCTTAAGGCAGGCCCTTAGTGAATGTTCCGCCACGTCCCCTGAACAGGATTCTCTCCTGAAGCGCTGT‑TTTCCCAAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACcac                                                                                                         <  2:375892/239‑1 (MQ=255)

                                                                                                                            aTCGCCTGTGTAAAGGGTGCCCCCAGCATCCAGCCCGCTGTCAGCAGGGCCTTATTTAATTTTACGCCACGTCCCCTGAATATGAATCTCTCCTGAAGCGGTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACcaca                                                                                                        <  2:1714403/239‑1 (MQ=255)

                                                                                                                                  tgtgTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGcc                                                                                                                                        >  2:2072818/1‑201 (MQ=255)

                                                                                                                                  tgtgTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCAt                                                                                                  <  1:942073/239‑1 (MQ=255)

                                                                                                                                         aaGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGaa                                                                                                                                                                                   >  1:265738/1‑151 (MQ=255)

                                                                                                                                              gccgccAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACtt                                                                                                                                            >  1:1548426/1‑185 (MQ=255)

                                                                                                                                               ccgccAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGaaa                                                                                                                                                                             >  1:444653/1‑151 (MQ=255)

                                                                                                                                               ccgccAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATccc                                                                                      >  2:2098927/1‑238 (MQ=255)

                                                                                                                                                  ccAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCa                                                                                  >  1:1952340/1‑239 (MQ=255)

                                                                                                                                                       aTACAGACCGCTGTCACCAGGGCCTTAGTTAATGTTCCGCCACGCCGCCTGAACAAGAATCTCTCCTGAAGCGCTGA‑CTCCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCt                                                                               <  1:914112/237‑1 (MQ=255)

                                                                                                                                                             aCCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACgg                                                                                                                      >  1:130547/1‑192 (MQ=255)

                                                                                                                                                             aCCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGCCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGc                                                                       >  1:341219/1‑239 (MQ=255)

                                                                                                                                                              ccGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACTTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCa                                                                      <  1:1497893/239‑1 (MQ=255)

                                                                                                                                                               cGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCaga                                                                                                                                     >  2:2016578/1‑175 (MQ=255)

                                                                                                                                                                gCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGata                                                                                                                                 >  2:826502/1‑178 (MQ=255)

                                                                                                                                                                gCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGata                                                                                                                                 <  1:826502/178‑1 (MQ=255)

                                                                                                                                                                 cTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCg                                                                                                                                          <  1:464731/168‑1 (MQ=255)

                                                                                                                                                                        caggcctgtAGTTAATGTTCCGCCTGCTCGCCTAACAATGCACCCCGCCAGAAGGACTGA‑CGTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACCGTGTGCTTATCCATCTGCCCCACTTCGACAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGTTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                            <  2:355274/231‑1 (MQ=255)

                                                                                                                                                                           ggCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAg                                                         >  1:1213225/1‑239 (MQ=255)

                                                                                                                                                                           ggCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAg                                                         >  2:769760/1‑239 (MQ=255)

                                                                                                                                                                            gCCTTAGTTAATGTTACGCCACGTCGCCTGACCATGACTCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                            <  1:605499/235‑1 (MQ=255)

                                                                                                                                                                              cTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCAGTGGAATCGAAAATTTGACTGTGGCCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATc                                                                                        >  1:1052866/1‑205 (MQ=255)

                                                                                                                                                                                       tGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                            >  2:1513207/1‑224 (MQ=255)

                                                                                                                                                                                       tGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCg                                                            <  1:1513207/224‑1 (MQ=255)

                                                                                                                                                                                       tGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGc                                              >  1:1096041/1‑238 (MQ=255)

                                                                                                                                                                                       tGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGc                                              >  1:744689/1‑238 (MQ=255)

                                                                                                                                                                                            ccccagtcgcccTGAACAGGATCCCCCCCTGAGGCGTCGA‑CTTGCCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGtt                                        <  2:976034/229‑1 (MQ=255)

                                                                                                                                                                                            cGCCCCGCCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTCCGCCAAACCAGCCGGTATCATCTACCGTACGCAGCGAAATGCCTTTCTCCATTGGAATCGAAAATTTGACCGTGAACTTATCCATCTGCCCCACTTCGCCAGCGAGATGAGTCCTCGATCTGGTCAACCACTGCCCATCGGTCATATCCAGCCACCTATCATCGCACCAGCCCTAGTAGGGATTTGTAGCACCGtt                                        >  2:1084161/1‑239 (MQ=255)

                                                                                                                                                                                            cGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGtt                                        >  2:1910954/1‑239 (MQ=255)

                                                                                                                                                                                             gCCATGACGCCGTAACATGACTCTCTCAAGAAG‑ACTGAGCTTCCCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGTCGTTATCCATTTGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTg                                       <  2:1650524/239‑1 (MQ=255)

                                                                                                                                                                                               cccgcccccTGACCAGGATCCTCCCCGGAGGCGCTTA‑CTTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCg                                     <  2:1096023/232‑1 (MQ=255)

                                                                                                                                                                                               cACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCg                                     <  1:1886974/239‑1 (MQ=255)

                                                                                                                                                                                               cACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCg                                     <  1:873675/239‑1 (MQ=255)

                                                                                                                                                                                                 cGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCggg                                   >  1:730786/1‑239 (MQ=255)

                                                                                                                                                                                                 cGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCggg                                   >  1:1101799/1‑239 (MQ=255)

                                                                                                                                                                                                                   ctcccCTGAAGGGCTGA‑TCTCGCCAACCCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCg                 <  2:709649/239‑1 (MQ=255)

                                                                                                                                                                                                                    tctcCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCGGGCACCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAggtccg                 >  2:939791/1‑233 (MQ=255)

                                                                                                                                                                                                                      tcCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCg              >  1:516153/1‑239 (MQ=255)

                                                                                                                                                                                                                      tcCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCg              >  1:505752/1‑239 (MQ=255)

                                                                                                                                                                                                                            aGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACg        <  1:1796041/239‑1 (MQ=255)

                                                                                                                                                                                                                                cTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGt      >  1:921783/1‑237 (MQ=255)

                                                                                                                                                                                                                                  gA‑CTTGGCCCACCCGGCCGTTATCATCTCCCGTACGCAGCGAAAGGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGTACtt  <  2:1952340/239‑1 (MQ=255)

                                                                                                                                                                                                                                  gA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCg                                                                                     <  2:274985/156‑1 (MQ=255)

                                                                                                                                                                                                                                  gA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCg                                                                                     >  1:274985/1‑156 (MQ=255)

CCCGGGCTATCAATAGCACTGATGGCAATACGGTATATCCGTGCGCTTTTGTTGTTATTCACTACACGTTTGCTGACAAAGTCAGTCTC‑GGACGGCAGCGAAAATGTCAGATTGCCGACCGAAATCGCCTGTGTCAAAGGTGCCGCCAGCATACAGACCGCTGTCAGCAGGGCCTTAGTTAATGTTACGCCACGTCGCCTGAACATGAATCTCTCCTGAAGCGCTGA‑CTTCGCCAAACCAGCCGTTATCATCTACCGTACGCAGCGAAATGGCGTTATCCATTGGAATCGAAAATTTGACTGTGGTCTTATCCATCTGCCCCACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGTACTT  >  NC_007779/304224‑304687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`