Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	3,777,742	C→T	16.0%	L311L (CTG→TTG)	Y75_RS18735 →	PTS transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	3,777,742	0	C	T	16.0%	74.3 / 3.0	58	L311L (CTG→TTG)	Y75_RS18735	PTS transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base C (29/16);  new base T (6/2);  total (38/20)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCACTTCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGC  >  NC_007779/3777507‑3777959                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    cctcTTCATCTACGGCCAGTTTTTCTTTGGTCCGGCAGCTTTTGAAGGCGGCATTCAGATGTCCTGGGCGCAGCATCTGCAAGATGTCTGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGa                                                                                                                                                                                                                                  <  2:1744834/236‑1 (MQ=255) ccacTTCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAGGGCGGCATTCAGATGTCCTGGGCGCAGCATCTGCAAGATGTCAGTGTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGa                                                                                                                                                                                                                                  <  1:935347/239‑1 (MQ=255)  cacTTCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑t                                                                                                                                                                                                                                <  1:1283454/239‑1 (MQ=255)     ttCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGA‑GTT‑G‑TTTA‑TTc                                                                                                                                                                                                                              >  1:2150711/1‑238 (MQ=255)     ttCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGATCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑CGG‑CTT‑G‑CTGA‑tta                                                                                                                                                                                                                              >  1:1611838/1‑237 (MQ=255)       cATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑TTGC‑ttttc                                                                                                                                                                                                                            >  2:765379/1‑235 (MQ=255)       cATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCg                                                                                                                                                                                                                           <  1:1081311/239‑1 (MQ=255)       cATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGCTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAACATCGGGCAAAA‑GTGGC‑GGG‑CTT‑G‑GTGA‑TTgcc                                                                                                                                                                                                                            >  2:435342/1‑238 (MQ=255)            aCGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑G‑TTGt‑tttacgcgcc                                                                                                                                                                                                                       >  2:654367/1‑230 (MQ=255)                    tttATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAGGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑T‑CGGA‑TTTGCCCAACCAT‑AA‑ctc                                                                                                                                                                                                             >  1:2135554/1‑236 (MQ=255)                       aTCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGT‑TTTCCCCAACCTT‑AA‑CC‑C‑CCac                                                                                                                                                                                                       >  1:592294/1‑238 (MQ=255)                            tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGT‑GTT‑G‑CCTG‑TTCCCGCCCCCCT‑AA‑CC‑C‑CCA‑TGCcgg                                                                                                                                                                                                 >  1:1284302/1‑239 (MQ=255)                            tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGA‑TTTCCGCCACCCT‑CA‑CCGG‑CCA‑Tgtgg                                                                                                                                                                                                  >  1:867040/1‑236 (MQ=255)                            tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTGT‑TTCCCCCCCCCCT‑TA‑CC‑G‑CCA‑CCCtgg                                                                                                                                                                                                 >  1:1476109/1‑239 (MQ=255)                              gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGT‑TTCCCCCAACCTC‑AA‑AC‑C‑CCC‑TGCtgggg                                                                                                                                                                                               >  1:1313743/1‑237 (MQ=255)                              gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTC‑G‑CCGA‑GTCTCCCCACCTT‑CA‑CC‑G‑CCC‑AGCtggtg                                                                                                                                                                                               >  1:1530451/1‑239 (MQ=255)                              gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTGGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGA‑CTT‑G‑CTGG‑TTCCCTCCACCAT‑AA‑TC‑G‑CCaacacggtg                                                                                                                                                                                                >  1:1900420/1‑231 (MQ=255)                                   gCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑TTGG‑TTTCCGCCACCCT‑TA‑CC‑G‑CCA‑CCCTggggggggg                                                                                                                                                                                          >  1:710443/1‑236 (MQ=255)                                                  ggcATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGGAGTACC‑GAACCGcccgg                                                                                                                                                                          >  1:537114/1‑235 (MQ=255)                                                    cATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑G‑CTGG‑ATTCCGCCAACTT‑AA‑AC‑A‑CCACTACGGGGGGGATTGACC‑GACCCgtggg                                                                                                                                                                           >  1:1352480/1‑233 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGGAAATCGGGTAAAA‑GTGGC‑GGG‑GCT‑G‑CCGG‑TTCCCCCCACCCT‑AA‑AC‑C‑CCA‑CGGGTGGGGGGATTACC‑GAAACGCC‑GGGATTTACCTTCCTGTTCa                                                                                                                                                        >  1:1562327/1‑239 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGGCCTT‑G‑CGGT‑TTCCCCCCACC‑T‑AA‑CC‑GACCA‑TCCTGGGGGGAATCA‑C‑GAACCGCC‑GGCAATTAGCTTTCTGttta                                                                                                                                                        >  1:1793172/1‑237 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGT‑TTCCCCCAACCCT‑AA‑CC‑C‑GCC‑TGCCGGGGGGGGTTAAC‑GGACCGCC‑GGGAATTTTCTTCCCGTTct                                                                                                                                                        >  1:370096/1‑238 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑TTGA‑TTTCCCCCCCCCT‑AA‑CC‑C‑CCC‑CGCAGGGGGGGAGTACC‑GAACCGCT‑GGAATTTTACTTCCTGTTCa                                                                                                                                                        >  1:132239/1‑239 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑TTGA‑TTTCCCCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGATTTCC‑GAACCGCC‑GGGATTTAACCTCCCGtt                                                                                                                                                          >  1:847954/1‑237 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGG‑TTTCCGCAACCCT‑AA‑CC‑C‑CCA‑TGCTGGGGGGGATTACC‑GAAACACC‑GGGATTTTACTTccctgtca                                                                                                                                                        >  1:1078832/1‑233 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTTCCGCCACCCT‑AA‑CC‑G‑CCA‑CGCTGGGGGGGAGTAAC‑GACCCGCT‑GGGATTTACCTTCCTGtt                                                                                                                                                          >  1:594504/1‑237 (MQ=255)                                                                   ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCAATGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTTC‑TTTACCCAACCCC‑AA‑AC‑G‑CGC‑AGCCGGGGGGAAGTTTC‑CAAGCCCC‑GGAATTTAACTTCCTTTTct                                                                                                                                                        >  1:522096/1‑238 (MQ=255)                                                                     cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTTA‑TTCCCCCACCCCT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGATTATC‑GCACCGCT‑GGGATTTTACTTTCCGTTCCtt                                                                                                                                                      >  1:733275/1‑239 (MQ=255)                                                                     cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTTCCCCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGAATTCC‑CAAACGCT‑GGAATTTTCCTTCCTCTTCAtt                                                                                                                                                      >  1:1289579/1‑239 (MQ=255)                                                                     cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑T‑CTGG‑TTCCCCCCACCCT‑AA‑CC‑G‑CCA‑CCCTGGGGGGGGATACCGGAACCCCG‑GGAATTTA‑CTTCCTCTTCCtt                                                                                                                                                      >  1:1659463/1‑239 (MQ=255)                                                                       cagcaTCTGAAAGGTTTCGGTTTGGGCGCGGAGCCGCTAAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTAAAAATTCTTTGGTGCCGTGGGCTTTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GGGGG‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCAttt                                                                                                                                                     <  1:199963/238‑1 (MQ=255)                                                                        agcaTCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGT‑GCT‑C‑CTGA‑CTGCTGCACCCCC‑AC‑AC‑G‑CAC‑TGCCTGTGGGAAATTAC‑GAACCCCT‑GGCATCTACCTTcacgttattttt                                                                                                                                                    >  2:2058808/1‑227 (MQ=255)                                                                        agcaTCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTGG‑TTTCCCCCCCCTT‑AA‑CC‑C‑CCA‑CTCCTGTGGGGAGTACC‑CCACCGCC‑GGGATTTATCCTCCCGTTCCTTTc                                                                                                                                                    >  1:2132500/1‑238 (MQ=255)                                                                               gCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑GTGA‑TTTCCCCAACCCT‑AA‑CC‑C‑CCC‑TTCTGGGGGGGATTAAC‑GAACCCCTCGGAATTT‑CCTTCCTTTTTCTTTCAcccgt                                                                                                                                              >  1:282827/1‑234 (MQ=255)                                                                                      ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGCGGGG‑CTT‑T‑CT‑A‑TTCCCCCCACCCT‑AA‑CC‑GCCCA‑GCCGGGGGGGATTTACG‑AAACCCCG‑GGATTTTTCCTT‑CTTTTTTTTTTACCATTGCTGttttt                                                                                                                                     >  1:698124/1‑237 (MQ=255)                                                                                      ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGA‑TTTCCCCACCCCT‑AA‑CC‑G‑CCA‑CGCTGGTGGGGGTTAGT‑GAACCCCC‑GGAGATTACCTTCCCCCTTCTTTCTCCGTCTCTGTTTgt                                                                                                                                     >  2:604548/1‑238 (MQ=255)                                                                                      ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTGA‑TTCCCGCCACCTT‑AA‑CC‑C‑CCA‑AGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTTCTGTTCCTTTCACCGTTTCTGttttc                                                                                                                                     >  1:1050594/1‑237 (MQ=255)                                                                                                         gtgtgaatccGTTGTCCCCGG‑AGGGGGTTTTGCCCTGGCGGGTTAACGAAAAATGTTTGGGGGCGTTGGGTTTTTTTTAGGGG‑T‑TTTTCTCCCCGCCCGCA‑CGGAAAACGGGGTAAAAGGGGGG‑GGG‑TTT‑G‑CTGC‑TCCCCGCACCTTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTggc                                                                                                                   <  2:698124/229‑1 (MQ=255)                                                                                                                    ttgtcCGCGGAGGGTGTTTTTGCCCTGCGCGTTAACTCAAAATTTTTGGGTGCTGTGGG‑TTTTTTTTA‑GGGTT‑TGTTCTTCCCT‑CCCCCCCCGAAAAACGGGTTAAA‑GGGGG‑GGGTCTT‑G‑‑TTA‑TTCCCCGAACCTT‑AACCC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTc                                                                                                       <  2:132239/239‑1 (MQ=255)                                                                                                                     tgtcCCGGGAAGGTGTTTTTGCCCGCCAGTGTAACAAAAAAATTTTTGGTGCGGTGGTATTTTTGTTA‑GGA‑T‑TTTCCTCCCCGGCCCCCCCGGAAAAT‑GGGTTAAA‑GGGGG‑GGG‑CTT‑GTGTAC‑TCCCCGAAACTTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTc                                                                                                       <  2:1193351/238‑1 (MQ=255)                                                                                                                                 ggtgtttcccccTGCCGGGCAACTAAAAACTGTTTGGGGCGGGGGGC‑TTTTTTTT‑GCG‑ATTTTCCTCCCCGGCCGCACC‑GAAAAGTGGGGAAAA‑GTGGG‑GGG‑GTTGG‑CTGT‑TCCCCCCAACTTA‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTa                                                                                         <  2:1289579/229‑1 (MQ=255)                                                                                                                                    gTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGGGCCGTGGGCTTTTCTTTG‑GCG‑A‑TGTCCTTCACTGCCGCACCGAAAAATGGGGTAAAA‑GTGGC‑GGG‑TTT‑G‑CTGA‑TTCCCGCAACCTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTAtct                                                                                      <  1:621766/238‑1 (MQ=255)                                                                                                                                     ttttCCCCGCCACGTTAACTAAAAAATGTGTGGTGCGGGGGGCTTTTCTTGA‑GGT‑G‑TTTTCTCCACCGCCGCACCGAAAATGGGGGTAAAG‑GTGGC‑GGG‑TTT‑G‑CTG‑‑TTCCCCGAACCTTAAA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATctct                                                                                    <  2:282827/239‑1 (MQ=255)                                                                                                                                               cACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑GTTA‑GTTCCTCCACCCT‑AA‑CA‑C‑CCC‑CGCTGGTGGGGAGTACC‑GAACCGCT‑GGAATTTATCTTCCTTTTCCTTTCACCTGTTCTGTTTTGGGTTCACGCCCTGCTGGCCGCCTCAATGTTGTGCCGA‑AAT‑TTTCTCTTttgtgtggt                                                                          >  2:1508316/1‑239 (MQ=255)                                                                                                                                                    tAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTGATTTCCCCCCCCCTA‑AA‑CC‑C‑CCA‑GCCGGGGGGGAATAACC‑GAACGCCT‑GGAGTTT‑CTTTTCTTTTCATTTTTACGCTGCTGTTTTTGGGACACCCCCCGCTGGCGGCCTCCCTGGCG‑TCCCG‑AATGTTGTTCTTTTGTGTTGTggggg                                                                     >  2:994636/1‑237 (MQ=255)                                                                                                                                                            aaaTCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCCCCACCTT‑AA‑CC‑G‑CCC‑TGCTGGGGGGGATTAAC‑GAACCGCT‑GGGATTTTTCTTCCTGTTTATTTTACCCTTGCTGTTTTCGGGACACCCCCTGCTGGCGGCCTCCATGTCG‑ACCGG‑AATGTATTTCTTTGGTGTGGGGGGGAAAACgggg                                                             >  1:1476386/1‑238 (MQ=255)                                                                                                                                                             aaTCTTTGGTGCCGTGGGTTTTTTTTTG‑GCG‑A‑TTTCCTTCCCTGCCCCCCCGAAAAAGCGGGAAAAA‑GGGGC‑GGG‑TTT‑G‑CTGA‑TCCCCGCAACTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCg                                                            <  1:1508316/239‑1 (MQ=255)                                                                                                                                                                       gCCGTGGGTTTTTTTTTG‑CCG‑T‑TGTCCTCCCCTCCCGCACCGAAAAATCGGGAAAAA‑GGGGG‑GGG‑TTT‑G‑CTGA‑TTCCCGCACCTTT‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GGACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGa                                                   <  1:1745199/238‑1 (MQ=255)                                                                                                                                                                             ggCTTTTTTTTA‑GCG‑A‑TGTCCTTCCCTGCCGCACCGGAAAAGCGGTTAAAG‑GTGGG‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACt                                             <  1:1355691/238‑1 (MQ=255)                                                                                                                                                                                       tA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTTA‑TTTCCCCCCCCCT‑AA‑CC‑C‑CCC‑TGCCGGTGGGGGGTATC‑GAACTGCC‑GGGATTTTCCTTCCCGTTTTTTTTACCGTTTCTGTTTTCGGGGCACGCCCTGCTGGCGGCCTCACTTTGT‑ACCGTCGGTGT‑TGTTTTTGGTGTGGGGGGGGGAATGGGTGGGGGTGGGATTTGCTAGGtttttct                                   >  2:149532/1‑235 (MQ=255)                                                                                                                                                                                         gggg‑A‑TGTCCTCCCCTCCCGCACCGAAAAACCGGGAAAAA‑GGGGC‑GGG‑CTT‑G‑CTGA‑TCCCCGAACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGc                                 <  1:994636/236‑1 (MQ=255)                                                                                                                                                                                                 gTACTCCCCTCCCCCACCGAAAAATGGGGTAAAA‑GTGGC‑GGG‑TTT‑G‑CGGA‑TCCCCGCACCCTT‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACt                           <  1:435342/239‑1 (MQ=255)                                                                                                                                                                                                    ctccccTGCCGCACCGAAAAATGGGGAAAAG‑GGGGG‑GGT‑TTT‑G‑TTGT‑CCCCCGCACCTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGa                        <  2:1458427/234‑1 (MQ=255)                                                                                                                                                                                                    cTTCACTGCCCACCCGGAAAATCGGGAAAAG‑GTGGC‑GGT‑CTT‑G‑CTGA‑TCCCCGCACCTTT‑CA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTGCCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGa                        <  1:945598/239‑1 (MQ=255)                                                                                                                                                                                                            ccGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CCT‑G‑CTGA‑TTCCCCCCACCCT‑AA‑AC‑C‑CCC‑TGCTTGGGGGGGCTACC‑GAACCCCA‑GGGAATTATCCTCCTTCTCCTTTCACCCCTTCTGGTTGCGGGACACGCCCCGCGGGCGGCCCCCCTATTG‑CCCGG‑CATTTATTTCTTTGGTGTGGGGGGGGAACTGGGCGGAGGGGTGCTTGAGTAGGTTTTTACGCAAAAATggcacccccag                >  2:1083783/1‑230 (MQ=255)                                                                                                                                                                                                                       aacgggttAAAG‑GGGGC‑GGG‑TTT‑C‑CTGA‑TCCCCCCACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCa     <  1:179084/232‑1 (MQ=255)                                                                                                                                                                                                                           ggtaaaaa‑GTGGG‑GGG‑CTT‑G‑TGGT‑TTCCCACACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTGCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCAcgc  <  2:1468214/234‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    CCACTTCATCTACGGACAGTTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGC  >  NC_007779/3777507‑3777959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑