Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	3,777,754	A→C	31.4%	T315P (ACC→CCC)	Y75_RS18735 →	PTS transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	3,777,754	0	A	C	31.4%	42.8 / 7.6	51	T315P (ACC→CCC)	Y75_RS18735	PTS transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base A (26/8);  new base C (9/8);  total (35/16)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCATTGGGTT  >  NC_007779/3777526‑3777992                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                        tttATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAGGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑T‑CGGA‑TTTGCCCAACCAT‑AA‑ctc                                                                                                                                                                                                                                              >  1:2135554/1‑236 (MQ=255)    aTCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGT‑TTTCCCCAACCTT‑AA‑CC‑C‑CCac                                                                                                                                                                                                                                        >  1:592294/1‑238 (MQ=255)         tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGT‑GTT‑G‑CCTG‑TTCCCGCCCCCCT‑AA‑CC‑C‑CCA‑TGCcgg                                                                                                                                                                                                                                  >  1:1284302/1‑239 (MQ=255)         tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGA‑TTTCCGCCACCCT‑CA‑CCGG‑CCA‑Tgtgg                                                                                                                                                                                                                                   >  1:867040/1‑236 (MQ=255)         tGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTGT‑TTCCCCCCCCCCT‑TA‑CC‑G‑CCA‑CCCtgg                                                                                                                                                                                                                                  >  1:1476109/1‑239 (MQ=255)           gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGT‑TTCCCCCAACCTC‑AA‑AC‑C‑CCC‑TGCtgggg                                                                                                                                                                                                                                >  1:1313743/1‑237 (MQ=255)           gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTC‑G‑CCGA‑GTCTCCCCACCTT‑CA‑CC‑G‑CCC‑AGCtggtg                                                                                                                                                                                                                                >  1:1530451/1‑239 (MQ=255)           gTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTGGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGA‑CTT‑G‑CTGG‑TTCCCTCCACCAT‑AA‑TC‑G‑CCaacacggtg                                                                                                                                                                                                                                 >  1:1900420/1‑231 (MQ=255)                gCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑TTGG‑TTTCCGCCACCCT‑TA‑CC‑G‑CCA‑CCCTggggggggg                                                                                                                                                                                                                           >  1:710443/1‑236 (MQ=255)                               ggcATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGGAGTACC‑GAACCGcccgg                                                                                                                                                                                                           >  1:537114/1‑235 (MQ=255)                                 cATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑G‑CTGG‑ATTCCGCCAACTT‑AA‑AC‑A‑CCACTACGGGGGGGATTGACC‑GACCCgtggg                                                                                                                                                                                                            >  1:1352480/1‑233 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGGAAATCGGGTAAAA‑GTGGC‑GGG‑GCT‑G‑CCGG‑TTCCCCCCACCCT‑AA‑AC‑C‑CCA‑CGGGTGGGGGGATTACC‑GAAACGCC‑GGGATTTACCTTCCTGTTCa                                                                                                                                                                                         >  1:1562327/1‑239 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGGCCTT‑G‑CGGT‑TTCCCCCCACC‑T‑AA‑CC‑GACCA‑TCCTGGGGGGAATCA‑C‑GAACCGCC‑GGCAATTAGCTTTCTGttta                                                                                                                                                                                         >  1:1793172/1‑237 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGT‑TTCCCCCAACCCT‑AA‑CC‑C‑GCC‑TGCCGGGGGGGGTTAAC‑GGACCGCC‑GGGAATTTTCTTCCCGTTct                                                                                                                                                                                         >  1:370096/1‑238 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑TTGA‑TTTCCCCCCCCCT‑AA‑CC‑C‑CCC‑CGCAGGGGGGGAGTACC‑GAACCGCT‑GGAATTTTACTTCCTGTTCa                                                                                                                                                                                         >  1:132239/1‑239 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑TTGA‑TTTCCCCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGATTTCC‑GAACCGCC‑GGGATTTAACCTCCCGtt                                                                                                                                                                                           >  1:847954/1‑237 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGG‑TTTCCGCAACCCT‑AA‑CC‑C‑CCA‑TGCTGGGGGGGATTACC‑GAAACACC‑GGGATTTTACTTccctgtca                                                                                                                                                                                         >  1:1078832/1‑233 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTTCCGCCACCCT‑AA‑CC‑G‑CCA‑CGCTGGGGGGGAGTAAC‑GACCCGCT‑GGGATTTACCTTCCTGtt                                                                                                                                                                                           >  1:594504/1‑237 (MQ=255)                                                ggCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCAATGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTTC‑TTTACCCAACCCC‑AA‑AC‑G‑CGC‑AGCCGGGGGGAAGTTTC‑CAAGCCCC‑GGAATTTAACTTCCTTTTct                                                                                                                                                                                         >  1:522096/1‑238 (MQ=255)                                                  cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTTA‑TTCCCCCACCCCT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGATTATC‑GCACCGCT‑GGGATTTTACTTTCCGTTCCtt                                                                                                                                                                                       >  1:733275/1‑239 (MQ=255)                                                  cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTTCCCCCACCTT‑AA‑CC‑G‑CCA‑TGCTGGGGGGGAATTCC‑CAAACGCT‑GGAATTTTCCTTCCTCTTCAtt                                                                                                                                                                                       >  1:1289579/1‑239 (MQ=255)                                                  cgcAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CGT‑T‑CTGG‑TTCCCCCCACCCT‑AA‑CC‑G‑CCA‑CCCTGGGGGGGGATACCGGAACCCCG‑GGAATTTA‑CTTCCTCTTCCtt                                                                                                                                                                                       >  1:1659463/1‑239 (MQ=255)                                                    cagcaTCTGAAAGGTTTCGGTTTGGGCGCGGAGCCGCTAAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTAAAAATTCTTTGGTGCCGTGGGCTTTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GGGGG‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCAttt                                                                                                                                                                                      <  1:199963/238‑1 (MQ=255)                                                     agcaTCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGT‑GCT‑C‑CTGA‑CTGCTGCACCCCC‑AC‑AC‑G‑CAC‑TGCCTGTGGGAAATTAC‑GAACCCCT‑GGCATCTACCTTcacgttattttt                                                                                                                                                                                     >  2:2058808/1‑227 (MQ=255)                                                     agcaTCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTGG‑TTTCCCCCCCCTT‑AA‑CC‑C‑CCA‑CTCCTGTGGGGAGTACC‑CCACCGCC‑GGGATTTATCCTCCCGTTCCTTTc                                                                                                                                                                                     >  1:2132500/1‑238 (MQ=255)                                                            gCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑GTGA‑TTTCCCCAACCCT‑AA‑CC‑C‑CCC‑TTCTGGGGGGGATTAAC‑GAACCCCTCGGAATTT‑CCTTCCTTTTTCTTTCAcccgt                                                                                                                                                                               >  1:282827/1‑234 (MQ=255)                                                                   ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGCGGGG‑CTT‑T‑CT‑A‑TTCCCCCCACCCT‑AA‑CC‑GCCCA‑GCCGGGGGGGATTTACG‑AAACCCCG‑GGATTTTTCCTT‑CTTTTTTTTTTACCATTGCTGttttt                                                                                                                                                                      >  1:698124/1‑237 (MQ=255)                                                                   ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑T‑CTGA‑TTTCCCCACCCCT‑AA‑CC‑G‑CCA‑CGCTGGTGGGGGTTAGT‑GAACCCCC‑GGAGATTACCTTCCCCCTTCTTTCTCCGTCTCTGTTTgt                                                                                                                                                                      >  2:604548/1‑238 (MQ=255)                                                                   ttCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTGA‑TTCCCGCCACCTT‑AA‑CC‑C‑CCA‑AGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTTCTGTTCCTTTCACCGTTTCTGttttc                                                                                                                                                                      >  1:1050594/1‑237 (MQ=255)                                                                                      gtgtgaatccGTTGTCCCCGG‑AGGGGGTTTTGCCCTGGCGGGTTAACGAAAAATGTTTGGGGGCGTTGGGTTTTTTTTAGGGG‑T‑TTTTCTCCCCGCCCGCA‑CGGAAAACGGGGTAAAAGGGGGG‑GGG‑TTT‑G‑CTGC‑TCCCCGCACCTTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTggc                                                                                                                                                    <  2:698124/229‑1 (MQ=255)                                                                                                 ttgtcCGCGGAGGGTGTTTTTGCCCTGCGCGTTAACTCAAAATTTTTGGGTGCTGTGGG‑TTTTTTTTA‑GGGTT‑TGTTCTTCCCT‑CCCCCCCCGAAAAACGGGTTAAA‑GGGGG‑GGGTCTT‑G‑‑TTA‑TTCCCCGAACCTT‑AACCC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTc                                                                                                                                        <  2:132239/239‑1 (MQ=255)                                                                                                  tgtcCCGGGAAGGTGTTTTTGCCCGCCAGTGTAACAAAAAAATTTTTGGTGCGGTGGTATTTTTGTTA‑GGA‑T‑TTTCCTCCCCGGCCCCCCCGGAAAAT‑GGGTTAAA‑GGGGG‑GGG‑CTT‑GTGTAC‑TCCCCGAAACTTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTc                                                                                                                                        <  2:1193351/238‑1 (MQ=255)                                                                                                              ggtgtttcccccTGCCGGGCAACTAAAAACTGTTTGGGGCGGGGGGC‑TTTTTTTT‑GCG‑ATTTTCCTCCCCGGCCGCACC‑GAAAAGTGGGGAAAA‑GTGGG‑GGG‑GTTGG‑CTGT‑TCCCCCCAACTTA‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTa                                                                                                                          <  2:1289579/229‑1 (MQ=255)                                                                                                                 gTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGGGCCGTGGGCTTTTCTTTG‑GCG‑A‑TGTCCTTCACTGCCGCACCGAAAAATGGGGTAAAA‑GTGGC‑GGG‑TTT‑G‑CTGA‑TTCCCGCAACCTA‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTAtct                                                                                                                       <  1:621766/238‑1 (MQ=255)                                                                                                                  ttttCCCCGCCACGTTAACTAAAAAATGTGTGGTGCGGGGGGCTTTTCTTGA‑GGT‑G‑TTTTCTCCACCGCCGCACCGAAAATGGGGGTAAAG‑GTGGC‑GGG‑TTT‑G‑CTG‑‑TTCCCCGAACCTTAAA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATctct                                                                                                                     <  2:282827/239‑1 (MQ=255)                                                                                                                            cACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑GTTA‑GTTCCTCCACCCT‑AA‑CA‑C‑CCC‑CGCTGGTGGGGAGTACC‑GAACCGCT‑GGAATTTATCTTCCTTTTCCTTTCACCTGTTCTGTTTTGGGTTCACGCCCTGCTGGCCGCCTCAATGTTGTGCCGA‑AAT‑TTTCTCTTttgtgtggt                                                                                                           >  2:1508316/1‑239 (MQ=255)                                                                                                                                 tAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑T‑CTGATTTCCCCCCCCCTA‑AA‑CC‑C‑CCA‑GCCGGGGGGGAATAACC‑GAACGCCT‑GGAGTTT‑CTTTTCTTTTCATTTTTACGCTGCTGTTTTTGGGACACCCCCCGCTGGCGGCCTCCCTGGCG‑TCCCG‑AATGTTGTTCTTTTGTGTTGTggggg                                                                                                      >  2:994636/1‑237 (MQ=255)                                                                                                                                         aaaTCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCCCCACCTT‑AA‑CC‑G‑CCC‑TGCTGGGGGGGATTAAC‑GAACCGCT‑GGGATTTTTCTTCCTGTTTATTTTACCCTTGCTGTTTTCGGGACACCCCCTGCTGGCGGCCTCCATGTCG‑ACCGG‑AATGTATTTCTTTGGTGTGGGGGGGAAAACgggg                                                                                              >  1:1476386/1‑238 (MQ=255)                                                                                                                                          aaTCTTTGGTGCCGTGGGTTTTTTTTTG‑GCG‑A‑TTTCCTTCCCTGCCCCCCCGAAAAAGCGGGAAAAA‑GGGGC‑GGG‑TTT‑G‑CTGA‑TCCCCGCAACTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCg                                                                                             <  1:1508316/239‑1 (MQ=255)                                                                                                                                                    gCCGTGGGTTTTTTTTTG‑CCG‑T‑TGTCCTCCCCTCCCGCACCGAAAAATCGGGAAAAA‑GGGGG‑GGG‑TTT‑G‑CTGA‑TTCCCGCACCTTT‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GGACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGa                                                                                    <  1:1745199/238‑1 (MQ=255)                                                                                                                                                          ggCTTTTTTTTA‑GCG‑A‑TGTCCTTCCCTGCCGCACCGGAAAAGCGGTTAAAG‑GTGGG‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACt                                                                              <  1:1355691/238‑1 (MQ=255)                                                                                                                                                                    tA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑GTT‑G‑CTTA‑TTTCCCCCCCCCT‑AA‑CC‑C‑CCC‑TGCCGGTGGGGGGTATC‑GAACTGCC‑GGGATTTTCCTTCCCGTTTTTTTTACCGTTTCTGTTTTCGGGGCACGCCCTGCTGGCGGCCTCACTTTGT‑ACCGTCGGTGT‑TGTTTTTGGTGTGGGGGGGGGAATGGGTGGGGGTGGGATTTGCTAGGtttttct                                                                    >  2:149532/1‑235 (MQ=255)                                                                                                                                                                      gggg‑A‑TGTCCTCCCCTCCCGCACCGAAAAACCGGGAAAAA‑GGGGC‑GGG‑CTT‑G‑CTGA‑TCCCCGAACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGc                                                                  <  1:994636/236‑1 (MQ=255)                                                                                                                                                                              gTACTCCCCTCCCCCACCGAAAAATGGGGTAAAA‑GTGGC‑GGG‑TTT‑G‑CGGA‑TCCCCGCACCCTT‑AC‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACt                                                            <  1:435342/239‑1 (MQ=255)                                                                                                                                                                                 ctccccTGCCGCACCGAAAAATGGGGAAAAG‑GGGGG‑GGT‑TTT‑G‑TTGT‑CCCCCGCACCTTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGa                                                         <  2:1458427/234‑1 (MQ=255)                                                                                                                                                                                 cTTCACTGCCCACCCGGAAAATCGGGAAAAG‑GTGGC‑GGT‑CTT‑G‑CTGA‑TCCCCGCACCTTT‑CA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTGCCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGa                                                         <  1:945598/239‑1 (MQ=255)                                                                                                                                                                                         ccGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CCT‑G‑CTGA‑TTCCCCCCACCCT‑AA‑AC‑C‑CCC‑TGCTTGGGGGGGCTACC‑GAACCCCA‑GGGAATTATCCTCCTTCTCCTTTCACCCCTTCTGGTTGCGGGACACGCCCCGCGGGCGGCCCCCCTATTG‑CCCGG‑CATTTATTTCTTTGGTGTGGGGGGGGAACTGGGCGGAGGGGTGCTTGAGTAGGTTTTTACGCAAAAATggcacccccag                                                 >  2:1083783/1‑230 (MQ=255)                                                                                                                                                                                                    aacgggttAAAG‑GGGGC‑GGG‑TTT‑C‑CTGA‑TCCCCCCACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCa                                      <  1:179084/232‑1 (MQ=255)                                                                                                                                                                                                        ggtaaaaa‑GTGGG‑GGG‑CTT‑G‑TGGT‑TTCCCACACCCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTGCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCAcgc                                   <  2:1468214/234‑1 (MQ=255)                                                                                                                                                                                                                                       tCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGCATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTCTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAACACTGGATCCCGATGTTCAGCCACCTCACGGTAATGATGCTGACCCAACTCGCCa         >  2:1535425/1‑239 (MQ=255)                                                                                                                                                                                                                                       tCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCa         >  1:607506/1‑239 (MQ=255)                                                                                                                                                                                                                                              aCCTT‑TA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCATTGGGtt  <  1:1803209/239‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                        TTTATCTTTGGTCCGGCAGCTGTTGAAGGCGGCATTCAGATGTACTGGGCGCAGCATCTGCAAGAGTTCAGTTTGAGCGCCGAGCCGCTGAAATCGTTGTTCCCGGAAGGCGGTTTTGCCCTGCACGGTAACTCAAAAATCTTTGGTGCCGTGGGCATTTCTTTA‑GCG‑A‑TGTACTTCACTGCCGCACCGGAAAATCGGGTAAAA‑GTGGC‑GGG‑CTT‑G‑CTGA‑TTCCCGCAACCTT‑AA‑CC‑G‑CCA‑TGCTGGTGGGAATTACC‑GAACCGCT‑GGAATTTACCTTCCTGTTCATTTCACCGTTGCTGTTTGCGGTACACGCCGTGCTGGCGGCCTCAATGTCG‑ACCGT‑AATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAGGTCTGATTGACTAGGTTTTACCGCAAAACTGGATCCCGATGTTCAGCAACCACGCGGATATGATGCTGACCCAAATCGCCATTGGGTT  >  NC_007779/3777526‑3777992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑