BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	1,521,793	C→G	11.1%	intergenic (+15/‑183)	curA → / → Y75_RS07665	NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,521,793	0	C	G	11.1%	302.6 / 4.6	135	intergenic (+15/‑183)	curA/Y75_RS07665	NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
Reads supporting (aligned to +/- strand): ref base C (44/73); new base G (12/4); total (56/79)
Fisher's exact test for biased strand distribution p-value = 6.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

TGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAA  >  NC_007779/1521555‑1522028

  cTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACAGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGATTCAAAGgcg‑gcgg                                                                                                                                                                                                                                                       >  1:1629691/1‑238 (MQ=255)

    gAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑aa                                                                                                                                                                                                                                                    >  1:533113/1‑239 (MQ=255)

     aaaaaaCGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑aa                                                                                                                                                                                                                                                    >  1:936681/1‑238 (MQ=255)

     aaaaaaCGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAAAGGCG‑GCGT‑aa                                                                                                                                                                                                                                                    >  1:728575/1‑238 (MQ=255)

             tATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGGTGATTAAAGTTTCAACGGCG‑GGGT‑AA‑G‑CCGCCATa                                                                                                                                                                                                                                         >  1:1019335/1‑239 (MQ=255)

               ttCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GGGT‑AA‑g‑ccgcccataa                                                                                                                                                                                                                                       >  2:851606/1‑234 (MQ=255)

                     tGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CTGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GCGG‑GA‑A‑CCGCCACacaaaacat                                                                                                                                                                                                                                 >  2:464163/1‑235 (MQ=255)

                     tGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGAAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGATTCAACGGCG‑GGGG‑AA‑A‑CCGCCAAAAACAttcc                                                                                                                                                                                                                                 >  1:938745/1‑236 (MQ=255)

                      gCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAATCCAg                                                                                                                                                                                                                                >  1:437006/1‑239 (MQ=255)

                            ttttcttgcggCTAAGGATT‑TGGTCACCCGCACCAATGTGTTTCGGGGGGAGATGGGGCAAGGGGGGAAAGAGGAAAAAATCCCCTACCGCGAAGAAATTACTGAGGGTTTAGAGAAGGCGCCACAGACGTTTATCGGCCTGCTGAAGGTTAAAACCTT‑CGGAAA‑AGGGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCG‑GCGA‑AA‑G‑CCGCCATAAAAATCCAGGCaaaa                                                                                                                                                                                                                          <  2:1232180/229‑1 (MQ=255)

                              ttattaTCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAATCCAGGCaaaaa                                                                                                                                                                                                                         >  1:1232180/1‑238 (MQ=255)

                               tattatCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGc                                                                                                                                                                                                                       <  1:274435/239‑1 (MQ=255)

                               tattatCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTAAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTCCCGCGAAGAAATTACTGACGTTTTAGAGAATGCGCCACAGCCGTTTATCGGCCTGCGGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGc                                                                                                                                                                                                                       <  1:1455076/239‑1 (MQ=255)

                                      gCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑A‑CCGCCCTAAAAAACCCGGGAAAAAACTtttttt                                                                                                                                                                                                                >  1:325018/1‑239 (MQ=255)

                                      gCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGGTGGTTAAAAATTCAAAGGCC‑GGGG‑GA‑G‑ACCCCATAAAAAACTAGGGAAAAAAATTAtttt                                                                                                                                                                                                                >  2:1894135/1‑239 (MQ=255)

                                                 tGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑Tttaatt                                                                                                                                                                                                    >  1:1494358/1‑239 (MQ=255)

                                                 tGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTACACGGCG‑GGGT‑AA‑A‑GCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑Tttaatt                                                                                                                                                                                                    >  1:1379736/1‑239 (MQ=255)

                                                 tGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GCGTAAA‑G‑CCGCCCTAAAACACAAGGAAAAAACCTtttttttgcttttaat                                                                                                                                                                                                      >  2:1382971/1‑229 (MQ=255)

                                                  ggTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTTAGC‑Tttaatta                                                                                                                                                                                                   >  1:1279503/1‑239 (MQ=255)

                                                      cccctagtcaaTGAGTTTCAGAGGGAGATGGGGAAATGAGTGAAAGAGTATAAATTCCACCACCGAGAAGAAATTGCGGGGGTTTTAGAGAAGGGGCCACAGAGTTTTTTCGGCTAGCTGAAGGGTAAAAACTT‑‑GGCAAGAGTGGTAATCCGCGTGAAGGGTGATGATTAAATTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATtaact                                                                                                                                                                                                <  2:1629691/229‑1 (MQ=255)

                                                      a‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GGGT‑AA‑G‑CCGCCATAAAAATCCAGGGAAAAAGCTTATTTTCAGC‑TTTAATtaaata                                                                                                                                                                                               >  1:278094/1‑239 (MQ=255)

                                                      a‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGAATAAAAGTTCAAAAGCG‑GCGT‑AA‑T‑ACCACATACAAATCCAGGCACAACACTTCATTTCAGA‑Cttaattaaatc                                                                                                                                                                                               >  2:1581474/1‑236 (MQ=255)

                                                         cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑A‑CCGCCCTAAAAAACCAGCGAAAAAACTTATTTTTAGC‑TTTTATtaactaa                                                                                                                                                                                              >  1:2049173/1‑238 (MQ=255)

                                                           aaaccATGGTTTTCAGGGGGTAGTGGGGGCATGTGTAAAAGAGTATAAAATACACTCCCGGGAAGAATTTACTGAGGGTTTAGAGAATGCCCCGCAGGCGTTTAGCGGCCTGCAGAAGGGTAAAATCTT‑GGGCAA‑AGTGGTGACCCGCGTGGCGGTTGATAATTAAAGTTTAATCGGCG‑GCGT‑AA‑G‑CCGTCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑Cca                                                                                                                                                                                          <  2:278094/236‑1 (MQ=255)

                                                                   aGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGGTTCAACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTTAGC‑TTTAATTAACTAA‑CCACCTTAttt                                                                                                                                                                                  >  1:1572114/1‑238 (MQ=255)

                                                                     tttCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGTTGATTAAAGTTTCCACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAAACCAGGCAAAAAAGTTATTTTTAAC‑Tttaattta                                                                                                                                                                                                  >  1:969979/1‑220 (MQ=255)

                                                                      ttCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGGTGGTGAAAGGTTTAACGGCG‑GGGG‑AA‑GTCTCCCCAAAAAATACAGGGAAAAAACTAATTTTTGGT‑TTTAATT‑ACTAA‑CCACATCACTGttt                                                                                                                                                                               >  2:1642098/1‑238 (MQ=255)

                                                                        aaggggggagtGGGGAAAGGGGAGAAAAGGAAAAAAACCCACTCCCGGGAAGAATTTCCTGAGGGTTAAGAGAAGGGCCCACAGACGTTTAGCGGCCGGCGGAAGGGTAAAATCTT‑CGGCAA‑GGTGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCACCGGCG‑GCAT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTctg                                                                                                                                                                             <  2:1323151/229‑1 (MQ=255)

                                                                                 aTGGGGCAATGGGTGAAAGAGGATAAAATCCTCTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGCCGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑TGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑T‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCtaat                                                                                                                                                                     <  1:963748/238‑1 (MQ=255)

                                                                                  tGGGGCAATGGGTGAAAGAGGATAAAATCCGCTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCtaat                                                                                                                                                                     <  1:234139/237‑1 (MQ=255)

                                                                                       cAAGGGGTGATATGGGATAAACTCCAGTGCCGCAAAGAATATATTGGTGTTGTAGCTAATGCTGCAGAGTGTTTTATGGGCATGATGAATTGTAAAAATTT‑GGATGA‑AGTTGTGATCCGGGAGGCGGGTGGTGAATAATTTTTCACGGGCG‑GTTT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTAGTTTCAGC‑GTTAATTAACTAA‑CCCCATCATTGTTCTGCTGCTAATAacaaca                                                                                                                                                              <  2:150929/239‑1 (MQ=255)

                                                                                         aTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCCGCCTAAAAATCCAAGGAAAAAACTTATTTTTTGC‑CTTAAATAACTAACCCACA‑CATTTTTCTTCTGCTAATAATAACaaa                                                                                                                                                            >  2:825956/1‑239 (MQ=255)

                                                                                               gAAAGAGGATAAAATCCACTACCGCGAAAAAATTACTGACGGTTTAGAGAATGCGCCCCAGACGTTTATCGGCCTGCTGAAGGTTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGa                                                                                                                                                       <  1:1382971/238‑1 (MQ=255)

                                                                                               gAAAGAGGATAAAATCCACACCCCCAAAGAAATTACTGACGGTTAAGAGAATGCACCACAGACTGTGAGCGGCCTGCTGAAGGGAAAAAGCTG‑CGACAA‑AGTGGGGATCCGAGTGGAGGGTGATGATTAAGTTTTCAACGGCG‑TGGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  1:1190128/239‑1 (MQ=255)

                                                                                               gAAAGAGGATAAAACCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  1:851606/239‑1 (MQ=255)

                                                                                               aaaaGAGGAAAAAACCCCCTCCCGCAAAGAAATTACGGACGGTTTAGAGAAGGCGACAAAGACGTTTATCGGCCTGCTGAAGGGTAAAAATTT‑CGGCAA‑AGTGGTGATCCGCGTGGGGGGTGATGATAAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  2:1279503/238‑1 (MQ=255)

                                                                                                aaggagaATAAAACCCACTACCGCGAAGAAGTTACTGACGGTTAAGAAAAGCCGCCACGGACGTTTAGCGGCCTGTTGAAGGGTAAAACCTT‑CGAAAA‑GGTGGTGATCCGCGTGGGGGGTGATGATAAAAGTTTCAACGGCG‑GCTT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  2:936681/235‑1 (MQ=255)

                                                                                                aaaGAGGAAAAAATCCACTCCCGCAAAGAAATTACTGCGGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGATAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  2:437006/238‑1 (MQ=255)

                                                                                                     ggAAAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGAAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGtt                                                                                                                                                <  1:531664/238‑1 (MQ=255)

                                                                                                       aTAAAACCCACTCCCGCGAAGAAATTACGGACGGTTAAGAGAATGCGCCCGGGTCGTTTAGCGGCGGGCTG‑AGGGTAAAAACTTGCGGAAG‑AGGGGTGATCCGGGGGGCGGGTAAAGATAAAATTTTCAACGGGG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTAt                                                                                                                                             <  2:1227361/239‑1 (MQ=255)

                                                                                                            aTCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATAAAAGTTTCAACGGCG‑CCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGatta                                                                                                                                        <  1:2124732/239‑1 (MQ=255)

                                                                                                              cccctcccGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCGGCTGAAGGGTAAAAACTT‑CGGCAA‑GGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  1:1894135/233‑1 (MQ=255)

                                                                                                              ccACTCCCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  1:270968/239‑1 (MQ=255)

                                                                                                              ccACTACCGGGAAGAAATTCATGAGGGTTTGGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTC‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  2:2042535/239‑1 (MQ=255)

                                                                                                              ccACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  1:1654705/239‑1 (MQ=255)

                                                                                                              ccACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCAACAGACGTTTATCGGCCGGCTGAAGGGAAAAAACTG‑GGAAAA‑AGGGGGGATCCGCGTGGAGGGTTATAATTAAAGTTTCAACGGGG‑GCAA‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  1:1208708/239‑1 (MQ=255)

                                                                                                                  tACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGATTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAAAAAAGGTGA‑GGGGGTTATGATTATTCACt                                                                                                                                  >  1:1585721/1‑239 (MQ=255)

                                                                                                                      gcaaagaaATAACGGAGGTTTGAGAGAAGGCGCCACAGACTTTTAGCGGCCTGATGAAGGGTAAAAACTT‑CGGAAA‑AGTGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTaa                                                                                                                              <  2:1585721/236‑1 (MQ=255)

                                                                                                                       cgAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCCTAAAAATCCAGGGAAAAAAGTTATTTTCAGC‑TTTAATTAACTAA‑CCACGACATTGTTTTGCTGCTTATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAAt                                                                                                                             >  1:1168226/1‑239 (MQ=255)

                                                                                                                       cgAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGGAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAAAAACAAAGGTGA‑GAGGTTTATGATTATTCACTTTAAt                                                                                                                             >  1:2042535/1‑239 (MQ=255)

                                                                                                                       cgAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAACTTATTTTCAGC‑TTTAATTAACTAA‑CCAcatca                                                                                                                                                                                     >  1:1329926/1‑184 (MQ=255)

                                                                                                                         aagaaATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTTACTTTAATac                                                                                                                           >  1:1257030/1‑239 (MQ=255)

                                                                                                                           aaaaTTACTGACGGTTTAGAGAAGGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAACCTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATacac                                                                                                                         <  1:1581474/238‑1 (MQ=255)

                                                                                                                             aaTTACTGACGGTTTAGAGACTGCGCCGCAGACGTTTGTCGCGCGGCTGAAGGGTAAAAGCTT‑CGGCAA‑AGGGGTGACCCGCGTGGCGGGTAATGATTAAAGTTCAAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCGCTTTAATacac                                                                                                                         <  2:500351/237‑1 (MQ=255)

                                                                                                                             aaTTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATacac                                                                                                                         <  1:443387/237‑1 (MQ=255)

                                                                                                                                  cTGAGGGTTAAGAGAATGGGCCACAGACGTTTACCGGCCTGGGGAGGGATAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGa                                                                                                                 <  2:325018/239‑1 (MQ=255)

                                                                                                                                       ggTTTAGAGAAGGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGGGTGGCGGGTGATGATTAAATTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCAc                                                                                                                                   <  1:1476489/217‑1 (MQ=255)

                                                                                                                                           tcgagaATCGGCCAGAGACGTTTGTCTGCCTGCTGAAGGATAAAAACGT‑CGGCAA‑AGTGGTTATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCt                                                                                                       <  2:1019335/237‑1 (MQ=255)

                                                                                                                                                   gcgcCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGG‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAACTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GGGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgca                                                                                              >  1:105512/1‑239 (MQ=255)

                                                                                                                                                   gcgcCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGGTTCAACGGCG‑GGGT‑AA‑A‑CCGCCCTAAAAATCCCGGCAAAAAACTTATTTTTCGC‑TTTTATTAACTAA‑AACCAACAATTGTCTCCTGCCAATAAAAACAAAAGGGAGGGGGTTTATATTTTTTCCTTTTAATA‑AC‑CAAGGGAATTTCCTTCTG‑ccttca                                                                                               >  2:600085/1‑235 (MQ=255)

                                                                                                                                                     gcCACAGACGTTTAGCGGCCTGCTGAAGGGTAAAACCTT‑CGGCAA‑AGTGGTGACCCGCGTGGGGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgcagg                                                                                            <  1:471043/239‑1 (MQ=255)

                                                                                                                                                     gcCACAGACGTTTAGCGGCCTGCTGAAGGGTAAAAACTC‑CGGAAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgcagg                                                                                            <  1:890742/239‑1 (MQ=255)

                                                                                                                                                     gacgcaGACGTTTGTCGGCCTGCGGAAGGATAAAATCTC‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgcagg                                                                                            <  2:105512/235‑1 (MQ=255)

                                                                                                                                                                      gCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGGAAAAAGCTTATTTTCAGC‑TTTAATTTACTAA‑CCACATCATTGTTCTGCTGCTAATAAAAACCAAGGTGA‑GGGGGTTATGATTATTTACTTTTATAAAC‑CAGGTGAA‑TTTCTTCTG‑ACATGCAGGCA‑GGGTTTG‑ACAGaaaa                                                                         >  1:532530/1‑239 (MQ=255)

                                                                                                                                                                      gCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGGGGTGATCCGCGTGGCGGGCGATGATTAAAGATTCAACAGCG‑GCGG‑AAGG‑CCGCC‑TAAAAAACCCGGGAAAAAACTTATTTTTCGC‑TTTTATTAACTAA‑CCAAAACATTGTTCTTCTGCTAATAAAAACAAAAGTGG‑GGGGTTTTTAAATATTCAATTTAAAAAAC‑CAGGTGGA‑TTACCTCTC‑ACATGTAGGCAGGGGTTGG‑A‑AGaaaa                                                                         >  2:1789356/1‑239 (MQ=255)

                                                                                                                                                                         tggtgAAGGGTAAAATCTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATTATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCa                                                                                                                                    <  1:1350225/179‑1 (MQ=255)

                                                                                                                                                                          gctgAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGAGCAGCTTAAAGGTTCAACGGCG‑GCGG‑GA‑A‑CCGCCATAAAAATCCAGGCAAAAATGTTATTTTCAGC‑TTTAATTAACTTA‑CCACATCATTTTTCTGCCGCTAAAAAACACAAAGGt                                                                                                                                                         >  1:112946/1‑161 (MQ=255)

                                                                                                                                                                           ctgAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgcaggca                                                                                          <  1:866438/219‑1 (MQ=255)

                                                                                                                                                                             gAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGGGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGa                                                                  <  1:970417/239‑1 (MQ=255)

                                                                                                                                                                             gAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGa                                                                  <  1:926656/239‑1 (MQ=255)

                                                                                                                                                                                 ggTAAAAACTT‑AGGAAA‑AGTGGTAATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑Cca                                                                                                                                                                                          <  1:1525609/121‑1 (MQ=255)

                                                                                                                                                                                   tAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑g                                                                                                                                                     <  1:1874228/155‑1 (MQ=255)

                                                                                                                                                                                     aaaaCTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAAC‑TTTAATTAAATAA‑ACAAATCATTGTTCTGCTGCTAATAAAAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGt                                                                                                                   >  1:854006/1‑186 (MQ=255)

                                                                                                                                                                                     aaaaCTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAAATTCAACAGCG‑GCGG‑CA‑G‑CCCCCCTAAAAATCCAGGGAAAAAAATTATTTTCATCTTTTAATTAA‑TAA‑ACACAAAATTGTTCTGCTGCTACAAACAAAAAAAGTGA‑GGGGGTTATTAGTATTTACTTTTATACAA‑CACAGGAA‑TTTCCTTTC‑TCATGTCAGCA‑GGGTTGGTGCGGAAAAAAC‑AGAAGG‑AATTa                                                         >  2:1144765/1‑239 (MQ=255)

                                                                                                                                                                                          tt‑CGGAAA‑AGTGGTGATCCGCGTGGCGGGTGATGATAAAATTTTCAAGGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  2:1806167/163‑1 (MQ=255)

                                                                                                                                                                                           t‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGa                                                                                                                                                       >  1:1559955/1‑146 (MQ=255)

                                                                                                                                                                                           t‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATAAAATTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGa                                                                                                                                                       <  2:1559955/146‑1 (MQ=255)

                                                                                                                                                                                           t‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCG‑GGGG‑AA‑A‑CCGCCCTAAAAAATCCGGCAAAAAAGTTATTTTTCGC‑TTTTATTAACTAA‑CCACAACATTTTTTTGCCTCCAATAACAAAAAAAGGGA‑GGGGGTTATTTATATTTTATTTAATACACACAGG‑GAA‑TTTCTTTTGCCCATACGGGC‑‑GGGTTTG‑AAAAAAAAAACACGAAGGAAATTaaaact                                                    >  2:878182/1‑237 (MQ=255)

                                                                                                                                                                                              ggCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑A‑CCGCCACAAAAATCCCGGCAAAAAACTTATTTTTCGC‑TTTTATTAACTAA‑CCACAACATTTTTTTGCTGCTAAAAACAAAAAAGGTGG‑GTGGtttttta                                                                                                                                           >  2:1874228/1‑151 (MQ=255)

                                                                                                                                                                                                    aGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑A‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAAATAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAAc                                            >  1:1984679/1‑239 (MQ=255)

                                                                                                                                                                                                      tggtgATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GGGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGa                                                                  <  1:1552901/216‑1 (MQ=255)

                                                                                                                                                                                                      tggtgATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTTTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATa                                                                                                                            >  1:1806167/1‑163 (MQ=255)

                                                                                                                                                                                                      tggtgATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATgcagg                                                                                            <  1:715930/192‑1 (MQ=255)

                                                                                                                                                                                                      gggtgATCCGCGTGGGGGGTGAAGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTGAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCAt                                                                                                 <  2:954888/186‑1 (MQ=255)

                                                                                                                                                                                                       ggtgATCCGCGTGGGGGGTGATGATAAAAGTTTAAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACggg                                         <  1:306386/239‑1 (MQ=255)

                                                                                                                                                                                                        gtgATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGattatt                                                                                                                                      <  1:410436/151‑1 (MQ=255)

                                                                                                                                                                                                        gtgATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACggg                                         <  1:884576/238‑1 (MQ=255)

                                                                                                                                                                                                             ccGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGaa                                                                                                                <  1:179923/167‑1 (MQ=255)

                                                                                                                                                                                                                 ggggCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGaa                                <  1:478584/236‑1 (MQ=255)

                                                                                                                                                                                                                  tGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AT‑A‑CCGCCCTAAAAAACCAGGCAAAAAACTTTTTTTTAGC‑TTTAATTAACTAA‑CCACATCATTGTTTTGCTGCTAATAAAAACAAAAGTGA‑GTGGGTTTTTATTATTTACTTTtata                                                                                                                            >  2:410436/1‑151 (MQ=255)

                                                                                                                                                                                                                       ggTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑AAAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAAAATGTc                         >  1:1189132/1‑239 (MQ=255)

                                                                                                                                                                                                                          agtagttAAAGTTTAAAGGGGG‑GCGT‑AA‑T‑CCGCCATAAAAAATCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAg                       <  2:1189132/233‑1 (MQ=255)

                                                                                                                                                                                                                               taaaaTTTTCACCGGCG‑GGTT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGAc                 <  2:532530/237‑1 (MQ=255)

                                                                                                                                                                                                                                agaatgttCAACCGCGTGCTT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGAc                 <  2:2040924/233‑1 (MQ=255)

                                                                                                                                                                                                                                   attttAAACGGCG‑GTTT‑AA‑A‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTg             <  2:623991/237‑1 (MQ=255)

                                                                                                                                                                                                                                   aGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTg             <  2:1140422/239‑1 (MQ=255)

                                                                                                                                                                                                                                       tCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACCAGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAAATAAACAAGGCCGTTTGACTCTTCaag         >  2:2129036/1‑237 (MQ=255)

                                                                                                                                                                                                                                        cAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGt        >  2:1906/1‑239 (MQ=255)

                                                                                                                                                                                                                                        cAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGAATCTGCAGGt        >  1:1071946/1‑239 (MQ=255)

                                                                                                                                                                                                                                               cgacgT‑AA‑T‑CCGCCATAAAAATCCAGGCAAAAATCTTATTTTCAGC‑TTTAATTAGCTGA‑CCAAATCATTGTTCTGCTGCTAATAACAGCAAAGGTAA‑GTGGATTATGATTATTCACTATAATACAC‑CAGGCTAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCCAAGG‑AATTAAAATTCCTGGAACGGGAAAAATGAAACATGTCACATCGACTCCACAGCTTgaaa   <  2:1379736/235‑3 (MQ=255)

TGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCA‑CCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTT‑CGGCAA‑AGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCG‑GCGT‑AA‑G‑CCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGC‑TTTAATTAACTAA‑CCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGA‑GTGGTTTATGATTATTCACTTTAATACAC‑CAGGTGAA‑TTCCTTCTG‑CCATGCAGGCA‑GGGTTGG‑ACAGAAAACACGCGAAGG‑AATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAA  >  NC_007779/1521555‑1522028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`