BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	260,444	T→A	9.1%	I278N (ATC→AAC)	proB →	glutamate 5‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	260,444	0	T	A	9.1%	391.0 / 3.9	151	I278N (ATC→AAC)	proB	glutamate 5‑kinase
Reads supporting (aligned to +/- strand): ref base T (33/99); new base A (13/3); total (46/105)
Fisher's exact test for biased strand distribution p-value = 1.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATAT  >  NC_007779/260207‑260667

  gcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCa                                                                                                                                                                                                                                    <  1:732880/238‑1 (MQ=255)

  gcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCa                                                                                                                                                                                                                                    <  1:932113/238‑1 (MQ=255)

    gATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCTGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACg                                                                                                                                                                                                                                  <  1:2104826/238‑1 (MQ=255)

         ccGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTa                                                                                                                                                                                                                               >  1:1981230/1‑236 (MQ=255)

         ccGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGTAGA‑t                                                                                                                                                                                                                           >  1:2089787/1‑239 (MQ=255)

                ccacGTTTCAGGCCTCGGAACTGGCGGGATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCAGGCCCGGGCGACTGCGCTTGAAAGCCGTAAACGATGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAggg                                                                                                                                                                                                                     <  2:1077481/235‑1 (MQ=255)

                   cGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGCGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAAACACGGGAGA‑TGAAGGGGCa                                                                                                                                                                                                                  >  1:157639/1‑238 (MQ=255)

                           gCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAAACAAGGGAGA‑TGGAGGGGCCACTgccgc                                                                                                                                                                                                          >  1:1611208/1‑238 (MQ=255)

                            ccgcGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTgccg                                                                                                                                                                                                           <  1:585986/233‑1 (MQ=255)

                            ccTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTgccg                                                                                                                                                                                                           <  1:54821/236‑1 (MQ=255)

                             cTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAAAATCGGGAGA‑AGAAGGGGGTACGgccgccc                                                                                                                                                                                                        >  2:1292671/1‑237 (MQ=255)

                                    cTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGaa                                                                                                                                                                                                <  2:676101/239‑1 (MQ=255)

                                         ggcATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCgg                                                                                                                                                                                           <  1:1315117/239‑1 (MQ=255)

                                          gcATGAGTACCAAATTGCAGGCCGCGGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCgg                                                                                                                                                                                           <  2:985372/238‑1 (MQ=255)

                                              gAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCt                                                                                                                                                                                      <  1:1359624/239‑1 (MQ=255)

                                                 tACCAAATTGCGGGCCGCGGACGTGGCTTGCCGTGCGGGTATGGAAACCATTATGGCCGCGGGCAGCAGGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTcc                                                                                                                                                                                    <  2:810883/238‑1 (MQ=255)

                                                        ttGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCa                                                                                                                                                                                                        <  1:1406871/211‑1 (MQ=255)

                                                         tGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGGAATCCCCGGAGA‑GGATGGGGGGCCGTCCGCCATTCTGGGACGTGGCCGCcccccctctgcg                                                                                                                                                                           >  2:1934454/1‑231 (MQ=255)

                                                         tGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATAACGGGAGA‑TGAAGGGGCAAACGGCGCCATTTCGGAACGCGGGCGGTCCCTTTTGCCg                                                                                                                                                                           >  1:2003378/1‑239 (MQ=255)

                                                               ccGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGaaa                                                                                                                                                                        <  1:907818/236‑1 (MQ=255)

                                                                  cTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGTAGA‑TGAAGGGGGAACTGCCGCCATTCTGGAACGCGGCCGGCCCCTGTTGCCGAAAGGCGtt                                                                                                                                                                  >  1:1684928/1‑239 (MQ=255)

                                                                         ggtttGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGc                                                                                                                                                            <  1:2138691/235‑1 (MQ=255)

                                                                             tGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGTAGA‑TGAAGGGGGAAATGCCGCCATTCTGGGACGCGGCAGCCCCCTGTTGCCGAAAGGCATTAAAAGCGTg                                                                                                                                                         >  1:1510877/1‑237 (MQ=255)

                                                                               CCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGGAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCCGGTTCCGGAAGGCATTAAAAGCGGGACTg                                                                                                                                                     <  1:98026‑M1/239‑1 (MQ=255)

                                                                                       tgaTCGAACCCAGTGTTGCCGCGGGCAGCAGGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGCGGGCTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAAGCACGGTGGA‑TGAAGGGGCGACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGGCTGGCAAttt                                                                                                                                              <  2:327460/236‑1 (MQ=255)

                                                                                            gACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAACCCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTcgcg                                                                                                                                        <  1:779386/239‑1 (MQ=255)

                                                                                            gACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTcgcg                                                                                                                                        <  1:737901/239‑1 (MQ=255)

                                                                                            gACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTCATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAAACACGGATGA‑TGCAGGGGTAACTGCCGCCACTCCGGAACGCGGACGCGCCCTATTTCCGACACGCATTTAAAGGGAGGCTGGCGATTtccagc                                                                                                                                         >  2:467169/1‑234 (MQ=255)

                                                                                              cacCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTgccg                                                                                                                                                                                                           <  1:1393599/170‑1 (MQ=255)

                                                                                                    tattGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGAAACTGCCGCCATTCTGGAAcgcg                                                                                                                                                                                            <  1:1167708/179‑1 (MQ=255)

                                                                                                        gCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAAACACGGGAGA‑CTGAGGGGGTACGGTCCCCCATCTGGACCGCGGCAGGCCCCTCGTGCCGAAAGGGAATAAAAAAGTGACCGGGAAAATCTCGCGCGGGGAA‑GT‑Cac                                                                                                                          >  2:46082/1‑238 (MQ=255)

                                                                                                           gcgGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTgccg                                                                                                                                                                                                           <  2:313967/157‑1 (MQ=255)

                                                                                                           gcgGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCt                                                                                                                                                                                      <  2:918759/178‑1 (MQ=255)

                                                                                                           gcgGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGGAGA‑TGAAGGGGCAACTGCCGCCCTTCTGGAACGCGGGCGCTCCCCGGTTCCGAAAAGCATTAAAAGCGGGACCGGGAATTTCTCGCGGGGTGAA‑GG‑CATcc                                                                                                                        >  1:26834/1‑238 (MQ=255)

                                                                                                           gcgGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAATTAAGGGAGA‑AGAGAGGGCAACTGCCGCCCTTCTTGCAAGACGCCGCTCCCCGTTGTCCACAGGCATCAAAAAAGGAGCTGGCCATTTTCCGCGGGGGGAA‑ggcggaa                                                                                                                         >  2:1687856/1‑232 (MQ=255)

                                                                                                            cgGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCACCATTCTGGAACGCGGCAGCTCCCTGTTGCCGaaa                                                                                                                                                                        >  1:432755/1‑191 (MQ=255)

                                                                                                                 agcaAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCAttt                                                                                                                  <  1:909197/238‑1 (MQ=255)

                                                                                                                    aggCCGGCGGTTATTGGTAATGTATGGGAAGTAATTCCCGTCGACCGGCTATCCCATGCCCAGGCGCCTCGGCTTGAAAACCGTAAACGCTGGATTTGCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑a                                                                                                              <  2:1550793/236‑1 (MQ=255)

                                                                                                                    aaGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACGGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑a                                                                                                              <  1:937699/238‑1 (MQ=255)

                                                                                                                                    gtgatgtgatGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGGAGGGGGCACCGCCGCCCTTCTGGAACGCGGCAGCTCCCCGTGTCCGAAAGGGCATAAAAACGGGACGGGCCAATTCACGCGAGGGGGA‑GA‑CATACGCATTTGC‑CACCTCCAAGGCcccggt                                                                                             >  2:1841909/1‑237 (MQ=255)

                                                                                                                                    gtgatgtgatGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGCGAAAACACCGTAGA‑TGAAGAGGGAACGGCCCCCCCTCTGGGAAGCGGCAGCGTCCCGTTGCGGCAAGGCATTTAAAGCGCGACTGGTACTTTATCGCGTGGTGTA‑GG‑CATCAGACTTTTC‑AACCTCCaacaggcgcac                                                                                             >  2:1571318/1‑230 (MQ=255)

                                                                                                                                     tgatgtgatgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAATCAAGGGAGA‑TGAAGGGGCAACGGGCGCCCTTCTGGGAACCGGCAGCCCCCCGTTGCCGAAAAGGATTTAAAACGTGCCTGGCAAACTCTCCCGCGGGGCA‑GTGCAT‑CGCCTTTGC‑AACCTCCGAAGCcgcggaa                                                                                            >  2:566454/1‑236 (MQ=255)

                                                                                                                                         gtgatgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGGAAACACGGGAGA‑TGAAAGGGCAACTGCCGCCCTTCTGGGACGCGGCAGCTCCCTTTTGCCGAAAAGCATTAAAAACGTGGCTGGCAATTTCTCGCGGTGTGGA‑GT‑CACTCGCCATTTC‑AACCCCGACAGCCCCG‑ACATCg                                                                                        >  2:716213/1‑238 (MQ=255)

                                                                                                                                         gtgatgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGa                                                                                                                                                                          <  1:574309/160‑1 (MQ=255)

                                                                                                                                            atgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCCTTCTGGGACGCGGCAGCTCCCTGTTGGCGGAAGGTATTAAAAGAGTGGCTGGCCATTTCTCCCGTGGGGGA‑GT‑CCTCCCCATTTGC‑AACCTCCAAGGCCGCG‑CTAACcccc                                                                                     >  2:1703364/1‑238 (MQ=255)

                                                                                                                                             tgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGc                                                                                                                                                   <  1:1682737/179‑1 (MQ=255)

                                                                                                                                             tgGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCa                                                                                    <  1:610642/238‑1 (MQ=255)

                                                                                                                                                   gCATTTCCGTCGGGACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGt                                                                              <  2:1749219/238‑1 (MQ=255)

                                                                                                                                                    cATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGt                                                                              <  1:618346/237‑1 (MQ=255)

                                                                                                                                                     aTTTCCGTCGGTGCGCTGTTCCCTGCCCCGGCGACACCGCTTGAAAACCGTAACCGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCCCGGTAGA‑GGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCa                                                                            <  2:1312005/238‑1 (MQ=255)

                                                                                                                                                     aTTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCa                                                                            <  1:1050653/238‑1 (MQ=255)

                                                                                                                                                      tttCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGGAGA‑TGAAGGGGCAACTGCCGCCCTTCCGGAACGCGGCAGCGCCCTGTTGCCGAAAGGGATTTAAAACGCGACTGGCAATTTCTCCCGTGGGGAA‑AT‑CATCCGCCTTTTC‑AACCTCGAAGGCCGCG‑ATATCCCCCACGGCCTccg                                                                           >  2:165696/1‑236 (MQ=255)

                                                                                                                                                      tttCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTCTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑GGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑GACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGt                                                                          <  2:475347/239‑1 (MQ=255)

                                                                                                                                                             cGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCa                                                                                                                                                                                                                                    <  1:2182338/83‑1 (MQ=255)

                                                                                                                                                              ggacggCTGTTCCATGCCCAGGCGACACCGCTGGAAAACCGTAAACGTGGGTTTTTCGGTGCGCCGCCGGCGGTTGAAAACCCGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTacaa                                                                  <  2:255390/234‑1 (MQ=255)

                                                                                                                                                              ggTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTacaa                                                                  <  1:1224065/239‑1 (MQ=255)

                                                                                                                                                              ggTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAATCACGGTAGA‑AGAAGGGGCCACCGCCGCCCTTCTGGAACGCGGCCGCCCCCCGTTGCCGCAAAGAAATAAAAAAGTGACTGGCAATTTCTCCCGTGGTGAAGGT‑CTTCC‑CATTTGCAAACCCCGAA‑GCCGCG‑AGATCGCCCCCCGCGCCCGTccgtataa                                                                  >  2:597660/1‑232 (MQ=255)

                                                                                                                                                                tACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGa                                                                                                                                                                          <  2:1260728/137‑1 (MQ=255)

                                                                                                                                                                 aCGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAg                                                               <  1:401417/239‑1 (MQ=255)

                                                                                                                                                                 aCGCGGTTCCAGGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCGGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAg                                                               <  2:2121593/239‑1 (MQ=255)

                                                                                                                                                                   gCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCg                                                             <  1:1841909/239‑1 (MQ=255)

                                                                                                                                                                   gCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCg                                                             <  1:354559/239‑1 (MQ=255)

                                                                                                                                                                       ttCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑a                                                                                                              <  1:1920638/187‑1 (MQ=255)

                                                                                                                                                                       ttCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGGAAACACGGGAGA‑TGAAGGGGGAACTGCCGCCCCTCTGGGACGCGGCCGCGCCCTGTTGCCGGAAAGCATTAAAAACGTGACTGGGAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AAACTCGGAGGCCGCG‑ATATCGCCCCCGGCGTCAGTCGTTACAACAGCGAAGc                                                         >  2:701247/1‑239 (MQ=255)

                                                                                                                                                                        tCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCa                                                        <  1:809730/239‑1 (MQ=255)

                                                                                                                                                                               cccggCGACTACGCTTGAAACCCGTAAACGCTGGATTTTCGGGGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGc                                                  <  2:2150926/235‑1 (MQ=255)

                                                                                                                                                                               ccAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGcc                                                 >  1:1242412/1‑239 (MQ=255)

                                                                                                                                                                                 aGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGCGAAAACACGGGTGATTGCGGTGGCGCCGAGCGCC‑TTCTCGGCGGCGGGAGCGCCCGGTTGCCGGAAAGGAATAAAAGCGAGAGCGGGAAGTTCTTGCCCGGGGAA‑GA‑CACCCGCAAATAC‑AATCTCCGAAGCCACGCTTACCGCCC‑CGGCATCAGCACTTACAACAAAGGTGCATTACGCCGt                                               >  2:755199/1‑239 (MQ=255)

                                                                                                                                                                                   gCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAGGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTa                                              <  1:1934247/238‑1 (MQ=255)

                                                                                                                                                                                    cGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTa                                              <  1:648941/237‑1 (MQ=255)

                                                                                                                                                                                    cGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTa                                              <  1:951804/237‑1 (MQ=255)

                                                                                                                                                                                       cTCCGCGTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGc                                          <  1:487572/238‑1 (MQ=255)

                                                                                                                                                                                        tCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCg                                                             >  1:1450402/1‑218 (MQ=255)

                                                                                                                                                                                        tCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCg                                                             <  2:1450402/218‑1 (MQ=255)

                                                                                                                                                                                        tCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGGGAAATCACGGGAGA‑TGAAGGGGCAACTGCCGCCCTTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGGATTAAAAGCGTGACTGGCAATTTCTCGCGGGGGGTA‑GT‑CATCCGCATTTGC‑AACACCGAAGACCGCG‑ATATCGCCCCCCGCGGCACTCGGTACAACCACGATGCCTTACGCAGCATTGCCg                                        >  2:1159739/1‑239 (MQ=255)

                                                                                                                                                                                                      gTAAACGCGGGATTTTCGGGGCGCCGCCGGCGGGTGAAATCCCGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCaa                          <  2:866820/239‑1 (MQ=255)

                                                                                                                                                                                                       tAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGGCACCACCCGCAAg                         >  1:237739/1‑239 (MQ=255)

                                                                                                                                                                                                           cGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAAt                     <  1:1295696/239‑1 (MQ=255)

                                                                                                                                                                                                            gCTGAATTTTGGGTGGGCCCGCCGCGGGGGAAATCGCGGTAGA‑GGAGGGGGGAACTGCCGCCATTCTGTAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GG‑CATCCGCATTTTC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACGCCACTCGCAAGAAAt                     <  2:1614450/238‑1 (MQ=255)

                                                                                                                                                                                                                gATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAAt                     <  1:1425317/234‑1 (MQ=255)

                                                                                                                                                                                                                 aTTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATg                <  1:943833/238‑1 (MQ=255)

                                                                                                                                                                                                                 aTTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATg                <  1:354510/238‑1 (MQ=255)

                                                                                                                                                                                                                 aTTTTCGGTGCGCCGCAGGCGGGTAAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATg                <  1:1448059/238‑1 (MQ=255)

                                                                                                                                                                                                                  ttttCTGGGCGCCTCCGGCGGGTGAAATCACGGTAGA‑TGAGGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCa              <  2:26834/239‑1 (MQ=255)

                                                                                                                                                                                                                  ttttCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCa              <  1:1045497/239‑1 (MQ=255)

                                                                                                                                                                                                                  ttttCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCa              <  1:1512371/239‑1 (MQ=255)

                                                                                                                                                                                                                  gtttCGGGGCGCCGCCGGCGGGTGAAATCACGGTCGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCa              <  2:936141/238‑1 (MQ=255)

                                                                                                                                                                                                                      gggggcgcCGCCGGCGGGTGAAATCACGGTCGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATAc          <  2:1611208/235‑1 (MQ=255)

                                                                                                                                                                                                                      cGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCa                                                                            >  1:542977/1‑173 (MQ=255)

                                                                                                                                                                                                                      cGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATAc          <  1:1830144/239‑1 (MQ=255)

                                                                                                                                                                                                                        gTGCGCCGCCGGCGGGTGAAATCCCGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACt         <  2:2003378/238‑1 (MQ=255)

                                                                                                                                                                                                                        gTGCGCCGCCGGCGGGGGAAATAACGGGCGA‑TGAAGGGGCCACTGCCGCCCTTCTGGAAGGCGGCAGCTCCCTGTTGCCGAAAAGCATTAAAAACGGGGCTGGCAATTTCTCGCGTGGTGGA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCCATGCATTACGCCGTACTTACGGACACCACTCGCACGAAAATGCTGCAATACTg        >  2:526199/1‑239 (MQ=255)

                                                                                                                                                                                                                         tGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTg        <  1:493992/238‑1 (MQ=255)

                                                                                                                                                                                                                          gcgcCGCGGGCGGGTGAAATCACGGTAGA‑TGAAGGGGAACCTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTgg       <  2:1962982/238‑1 (MQ=255)

                                                                                                                                                                                                                          gcgcCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACGGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTgg       <  1:1118567/238‑1 (MQ=255)

                                                                                                                                                                                                                            gccgccGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCCGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGa     <  1:695897/238‑1 (MQ=255)

                                                                                                                                                                                                                              cgccgGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat  <  1:785876/239‑1 (MQ=255)

                                                                                                                                                                                                                              cgccgGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGata   <  2:1395923/238‑1 (MQ=255)

                                                                                                                                                                                                                                        tGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCCTTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTgg       >  1:2013792/1‑224 (MQ=255)

                                                                                                                                                                                                                                        tGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTgg       <  2:2013792/224‑1 (MQ=255)

GCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACGCTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGA‑TGAAGGGGCAACTGCCGCCATTCTGGAACGCGGCAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAA‑GT‑CATCCGCATTTGC‑AACCTCGAAGGCCGCG‑ATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATAT  >  NC_007779/260207‑260667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`