BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	547,831:1	+G	100%	coding (251/261 nt)	Y75_RS02655 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	547,831	1	.	G	97.3%	538.2 / 4.9	150	coding (251/261 nt)	Y75_RS02655	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (72/74); total (75/75)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 165 total aligned reads displayed.

GTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCG  >  NC_007779/547593‑548062

 tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTTGTCGC‑GCTCGTCCACACTGCCTGGCTGTGCAACCGGCTTCTTCACTTATCTGCGAACTAAACGAGGGCCAAACATTGCTTCCCGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGTCCAATGTAAGGGGCGTGCGTCGGCGTATGTCGGTTCGATGGTTGGGCGAAAGAAGAAGCGCAGGCGGGGGCACAAATGGGG‑CA‑g                                                                                                                                                                                                                                          >  2:298608/1‑239 (MQ=255)

 tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGCACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAGGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTAGAAGGATGGGCGGCAGATGAAGCGCAGGCGCTGGCCATACGGGAG‑CA‑g                                                                                                                                                                                                                                          >  2:124000/1‑239 (MQ=255)

 tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g                                                                                                                                                                                                                                          >  1:962601/1‑239 (MQ=255)

 tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g                                                                                                                                                                                                                                          >  1:1474578/1‑239 (MQ=255)

 tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g                                                                                                                                                                                                                                          >  1:1437420/1‑239 (MQ=255)

 tGGCGCAAGCCAATGAGGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCTGCCAATGCGCTGGCAGGAGATGACCGGACACATCACAGGGGCGGGCACGGGCGCATGTCTGTTCGAAGGCTGGGCGAAAGAGTGAGCGCAGGCGGGGGCAATACTGGAC‑CA‑g                                                                                                                                                                                                                                          >  2:1160707/1‑239 (MQ=255)

     gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g‑gggg                                                                                                                                                                                                                                     <  2:1636549/239‑2 (MQ=255)

          ccAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g‑ggggaagt                                                                                                                                                                                                                                 <  2:1738912/238‑6 (MQ=255)

          ccAATGCTGCGGTTATCGAACAAATT‑GTCGCGGTTCGTCCACGCTGGCTGGATGTGCAACCGGCTTCTTCACTCATCAGCGAACTAAACGAGGGCAAAACGCTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTg                                                                                                                                                                                                                                 <  2:1000335/239‑1 (MQ=255)

                   cGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCACCCGGCTTCTTCACTTCTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCCATGCGCTGGCAGGAGATGACCGGACCCATGAACGGGGCGTGCGTGGGCGCATGTCTGTTCGCAGGTTGGGCGAACGCTGACGCGCAGGCGCTGGCCATACTGGAGCCG‑G‑GGGAAGTGAACTTCAt                                                                                                                                                                                                                         >  1:569093/1‑238 (MQ=255)

                     gTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g‑ggggaagt                                                                                                                                                                                                                                 <  1:516219/227‑6 (MQ=255)

                     gTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑g‑ggggaagt                                                                                                                                                                                                                                 >  2:516219/1‑222 (MQ=255)

                       tATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCt                                                                                                                                                                                                                     <  1:1841011/238‑1 (MQ=255)

                         tCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGt                                                                                                                                                                                                                  <  2:479902/239‑1 (MQ=255)

                                       cgc‑gCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACGTCTCCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                                                                                                                                                                                                    <  2:1676244/239‑1 (MQ=255)

                                              cGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                                                                                                                                                                              >  1:858446/1‑239 (MQ=255)

                                                  cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                                                                                                                                                                          <  1:365259/239‑1 (MQ=255)

                                                      ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg                                                                                                                                                                                      <  1:361763/239‑1 (MQ=255)

                                                       tggctggATGTGCACCCGGGTTCTTCACTTATCAGGGAACTAACCGAGGGCAAAACTCTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  2:1474578/239‑1 (MQ=255)

                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  1:907929/239‑1 (MQ=255)

                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  1:871067/239‑1 (MQ=255)

                                                       tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  2:858446/239‑1 (MQ=255)

                                                       gggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTGCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCACGAAAGGGGCTTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  2:421587/238‑1 (MQ=255)

                                                         gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     >  2:1225700/1‑237 (MQ=255)

                                                         gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  1:1225700/237‑1 (MQ=255)

                                                          ctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                                                                                                                                                                    >  2:834235/1‑237 (MQ=255)

                                                           tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                                                                                                                                                 >  2:1011736/1‑239 (MQ=255)

                                                           tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                                                                                                                                                 >  1:169033/1‑239 (MQ=255)

                                                             gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                                                                                                                                                                                <  2:1137973/238‑1 (MQ=255)

                                                              aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt                                                                                                                                                                              >  2:110523/1‑239 (MQ=255)

                                                                 tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                                                                                                                                                                                                    >  1:1011665/1‑214 (MQ=255)

                                                                   cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                                                                                                                                                                    <  2:384112/228‑1 (MQ=255)

                                                                   cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                                                                                                                                                                    >  1:384112/1‑228 (MQ=255)

                                                                        ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCa                                                                                                                                                                    >  1:1042698/1‑239 (MQ=255)

                                                                          cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                                                                                                                                                                   <  1:1106488/238‑1 (MQ=255)

                                                                           ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTa                                                                                                                                                                 >  1:1406666/1‑239 (MQ=255)

                                                                               tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGcc                                                                                                                                                             <  2:558055/239‑1 (MQ=255)

                                                                                cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCg                                                                                                                                                            >  2:10717/1‑239 (MQ=255)

                                                                                   ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGAATTACATCTGCCAGTATGCCGac                                                                                                                                                          >  2:246731/1‑237 (MQ=255)

                                                                                        aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggt                                                                                                                                                    >  1:1258363/1‑239 (MQ=255)

                                                                                        aGCGAACTAAACGAGGGCAAAACACTGCCTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATTTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtg                                                                                                                                                      <  1:298608/237‑1 (MQ=255)

                                                                                          cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                     <  1:80228/204‑1 (MQ=255)

                                                                                          cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggtgg                                                                                                                                                  >  1:102204/1‑239 (MQ=255)

                                                                                             aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTggtgggtg                                                                                                                                               >  2:740792/1‑239 (MQ=255)

                                                                                             aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTg                                                                                                                                               >  1:1771785/1‑239 (MQ=255)

                                                                                                 aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggt                                                                                                                                                    <  2:1697415/230‑1 (MQ=255)

                                                                                                       ggCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACtt                                                                                                                                                                                                                            <  1:837172/152‑1 (MQ=255)

                                                                                                           aaaCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGAc                                                                                                                                 >  1:1625958/1‑239 (MQ=255)

                                                                                                            aaCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACg                                                                                                                                >  1:1523270/1‑239 (MQ=255)

                                                                                                                 tGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAAc                                                                                                                           <  2:1823436/239‑1 (MQ=255)

                                                                                                                    ttCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCgg                                                                                                                        <  1:1160707/239‑1 (MQ=255)

                                                                                                                      cACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGATCTTCa                                                                                                                                                                                                                          <  1:656455/139‑1 (MQ=255)

                                                                                                                      cACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCa                                                                                                                                                                                                                          >  2:656455/1‑139 (MQ=255)

                                                                                                                      cACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc                                                                                                                                                                                    <  1:1713113/177‑1 (MQ=255)

                                                                                                                        cGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGt                                                                                                                    >  1:1528301/1‑239 (MQ=255)

                                                                                                                        cGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGGATTACTTCTGCCAGTATGCCGAAGCTGGTGGTTGAGAATGTGACCGGAGGCAACCGTGCgg                                                                                                                    >  2:1503944/1‑238 (MQ=255)

                                                                                                                             ggCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTg                                                                                                                >  2:1255201/1‑238 (MQ=255)

                                                                                                                             ggCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGCGCGTGGGCGCATGTCTGTTCGACGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTg                                                                                                                >  2:355731/1‑238 (MQ=255)

                                                                                                                                cgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAAc                                                                                                            <  2:384031/239‑1 (MQ=255)

                                                                                                                                         gcTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGaa                                                                                                   >  1:691293/1‑239 (MQ=255)

                                                                                                                                                 gagaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCg                                                                                            >  1:1326427/1‑238 (MQ=255)

                                                                                                                                                  ggaTGACCGGACCCAGGAAAGGGGCGTGCGTGGGCGAATGTCTGTTCGAAGGTTGGGCGAAAGAAGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAAGTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCgg                                                                                           <  2:1771785/237‑1 (MQ=255)

                                                                                                                                                     tGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCa                                                                                         <  1:124000/237‑1 (MQ=255)

                                                                                                                                                       aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCCATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCg                                                                                                                     <  2:1084722/207‑1 (MQ=255)

                                                                                                                                                       aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCt                                                                                                                                                                                                                     <  1:298454/111‑1 (MQ=255)

                                                                                                                                                       aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCg                                                                                                                     >  1:1084722/1‑207 (MQ=255)

                                                                                                                                                       aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGCCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCt                                                                                                                                                                                                                     >  2:298454/1‑111 (MQ=255)

                                                                                                                                                         cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGa                                                                                  >  1:983145/1‑239 (MQ=255)

                                                                                                                                                            aCCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGc                                                                               >  1:1128835/1‑239 (MQ=255)

                                                                                                                                                                 tGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGCAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACGGACGGCAACCGGGCGAACTGCAACCTCAACGAAGGTATCGGCCAG‑GTGACGGGtttt                                                                          >  2:441347/1‑239 (MQ=255)

                                                                                                                                                                        ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCt                                                                    <  1:1616218/238‑1 (MQ=255)

                                                                                                                                                                          cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAAc                                                                                                                                          <  1:1742453/167‑1 (MQ=255)

                                                                                                                                                                          cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAAc                                                                                                                                          >  2:1742453/1‑167 (MQ=255)

                                                                                                                                                                          cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTAc                                                                 >  1:1322964/1‑239 (MQ=255)

                                                                                                                                                                          cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTAc                                                                 >  2:43534/1‑239 (MQ=255)

                                                                                                                                                                            tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACg                                                                >  1:1541716/1‑238 (MQ=255)

                                                                                                                                                                            tgcgtgGGCGCATGTCTGCTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGACACCATGTGAATGCCGTCGGGCCAATGTGCGGAATTACTTCTGCCAGTATGCCGACGCTGGTGGTTGAGAACGTGACCGACGACAACCGGGCGCACTGCACACTCAACGAAGGAATCGGCAAT‑GTGATGCGATTTGGCGCTTCCgg                                                               >  2:1095027/1‑239 (MQ=255)

                                                                                                                                                                              cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGGATCGGCAAAGGTGA‑GCGTTTTGGCGCTTACggag                                                             >  2:1358449/1‑237 (MQ=255)

                                                                                                                                                                               gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCg                                                             <  1:1144593/238‑1 (MQ=255)

                                                                                                                                                                                    cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACg                                                                                                     >  1:421762/1‑194 (MQ=255)

                                                                                                                                                                                           cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGa                                                 <  2:1437420/238‑1 (MQ=255)

                                                                                                                                                                                             gctCGACGGTTGGGCGATAGATGAAGCGCAGGCGCCGGCAATACTGGAG‑CA‑GTGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGAATTCCTTCGGCCAGTATGCGGATGCTGGCGGTTGAGGACGGGAGCGACGGCAACCGGGTGTACAGCCACCCCAACGAAGGATCCGGCAAC‑GAGATGCGTATTACCGCTTACGCCGAACACGTTCTGTCt                                               >  2:1575568/3‑238 (MQ=255)

                                                                                                                                                                                             gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTa                                                                  <  1:1173655/219‑1 (MQ=255)

                                                                                                                                                                                             gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTa                                                                  >  2:1173655/1‑219 (MQ=255)

                                                                                                                                                                                              ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGt                                                                                                                    <  1:1590150/169‑1 (MQ=255)

                                                                                                                                                                                                   aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAg                                                          >  2:158710/1‑221 (MQ=255)

                                                                                                                                                                                                   aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAg                                                          <  1:158710/221‑1 (MQ=255)

                                                                                                                                                                                                             aaaaATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTGATCGCCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATgcg                              <  1:1542676/239‑1 (MQ=255)

                                                                                                                                                                                                              aaGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATgcg                              <  1:924725/238‑1 (MQ=255)

                                                                                                                                                                                                               aGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGcgcg                            <  1:1060283/239‑1 (MQ=255)

                                                                                                                                                                                                                 aTGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGcgcg                            <  2:962601/237‑1 (MQ=255)

                                                                                                                                                                                                                  tGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                          <  2:1322964/238‑1 (MQ=255)

                                                                                                                                                                                                                  tGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                          <  2:1517492/238‑1 (MQ=255)

                                                                                                                                                                                                                  tGAAGCGCAGGCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                          <  2:86449/238‑1 (MQ=255)

                                                                                                                                                                                                                           ggCGCTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATg                 >  1:1655235/1‑238 (MQ=255)

                                                                                                                                                                                                                             cgcTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGcc               <  1:1358449/238‑1 (MQ=255)

                                                                                                                                                                                                                             cgcTGGAAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGcc               <  2:691293/238‑1 (MQ=255)

                                                                                                                                                                                                                               cTGGCAATACTGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCg                                                             >  2:1622370/1‑190 (MQ=255)

                                                                                                                                                                                                                                       ccTGGAG‑CAGG‑GGGACGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCCTCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcg    <  2:1358606/238‑1 (MQ=255)

                                                                                                                                                                                                                                         tGGAG‑CAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGcgcg  <  1:1095027/239‑1 (MQ=255)

GTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGC‑GCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAA‑GTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCG  >  NC_007779/547593‑548062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`