Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,371,2710A.98.3% 259.0 / ‑3.2 59pseudogene (183/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (58/0);  total (58/1)
Fisher's exact test for biased strand distribution p-value = 1.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0077794,371,2720A.98.3% 259.0 / ‑3.2 59pseudogene (182/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (58/0);  total (58/1)
Fisher's exact test for biased strand distribution p-value = 1.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTA  >  NC_007779/4371037‑4371502
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                      
tCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaata                                                                                                                                                                                                                                     >  1:1764508/1‑237 (MQ=255)
    cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                  >  1:70121/1‑233 (MQ=255)
    cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                  >  1:1492022/1‑233 (MQ=255)
    cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                  >  1:943129/1‑233 (MQ=255)
    cgcgGATACCACTGCAGTAGACAGCGGACGGCAAGGATTACCGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa                                                                                                                                                                                                                                        >  1:398646/1‑232 (MQ=255)
     gcgGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcggc                                                                                                                                                                                                                                >  1:1287263/1‑232 (MQ=255)
                       gACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                                                                                                                                                                                            >  1:827714/1‑239 (MQ=255)
                          aGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCAAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                         >  1:817174/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:1148585/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:1195315/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:1843323/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:1483211/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAAATTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCCCGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGAACGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:268320/1‑239 (MQ=255)
                            cGGACGGCAAGGTTTAACGCCTTGGTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCAAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGACACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgaga                                                                                                                                                                                                       >  1:1829342/1‑239 (MQ=255)
                                                 ttGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGAGGTTa                                                                                                                                                                                   >  1:1157930/1‑238 (MQ=255)
                                                 ttGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGGTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTa                                                                                                                                                                                   >  1:1005159/1‑238 (MQ=255)
                                                          cGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTa                                                                                                                                                                          >  1:269457/1‑238 (MQ=255)
                                                                          aCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAg                                                                                                                                                         >  2:2249900/1‑239 (MQ=255)
                                                                          aCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGAAAg                                                                                                                                                         >  2:1028635/1‑239 (MQ=255)
                                                                               ggggggggcgagagagTGGTTGCCAGTA‑CAGAAAAGGATGGAAAGGAAATGGGTCAGGGGCGGGGGGGGGATGGT‑TTTTTTTGTGGGTTGCGGGCGCACCATTTTTTGTGTGTGATAAACGAGATAGCCCGCCCCGCCAGCAACCTGGAGGGC‑‑AAAAAGGGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAAcc                                                                                                                                                     <  2:1157930/228‑1 (MQ=255)
                                                                                  tgtCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGc                                                                                                                                                  >  1:1249432/1‑238 (MQ=255)
                                                                                  tgtCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGc                                                                                                                                                  >  1:267638/1‑238 (MQ=255)
                                                                                  tgtCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGc                                                                                                                                                  >  1:543537/1‑238 (MQ=255)
                                                                                    tCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAc                                                                                                                                                >  1:2067127/1‑238 (MQ=255)
                                                                                                tGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGACCGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCg                                                                                                                                      >  2:2086028/1‑236 (MQ=255)
                                                                                                 gTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCt                                                                                                                                   >  2:1427656/1‑238 (MQ=255)
                                                                                                                    gATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTAtt                                                                                                                 >  1:257052/1‑238 (MQ=255)
                                                                                                                           aGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAAt                                                                                                                                         >  1:395419/1‑207 (MQ=255)
                                                                                                                               aaTAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATa                                                                                                                                                                 >  1:58269/1‑179 (MQ=255)
                                                                                                                                   ggTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGGTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCAAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCAAATCTGgcg                                                                                                  >  1:381316/1‑238 (MQ=255)
                                                                                                                                    gTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGgcgc                                                                                                 >  2:948679/1‑238 (MQ=255)
                                                                                                                                       aCGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAAt                                                                                                                                         >  1:412014/1‑195 (MQ=255)
                                                                                                                                       aCGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCaa                                                                                             >  1:693036/1‑239 (MQ=255)
                                                                                                                                       aCGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCA‑CCCCAATCCGCCTGCAAACCCAATACCTATTCCCCCCAATCCGGCGCCCaag                                                                                            >  2:444703/1‑239 (MQ=255)
                                                                                                                                              gTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCCGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATc                                                                                      >  1:1606819/1‑239 (MQ=255)
                                                                                                                                                   aGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGTTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCa                                                                                  >  2:629165/1‑238 (MQ=255)
                                                                                                                                                     gATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCa                                                                               >  2:1712587/1‑239 (MQ=255)
                                                                                                                                                         gTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACTAATCCCCCCCAACCTGACCCCCAAGAAGATCGCCACCaaa                                                                             >  1:1765888/1‑237 (MQ=255)
                                                                                                                                                         gTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCaaa                                                                             >  1:535691/1‑237 (MQ=255)
                                                                                                                                                         gTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCaaa                                                                             >  1:1626499/1‑237 (MQ=255)
                                                                                                                                                                  ttCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATAc                                                                                                                      >  1:1314708/1‑187 (MQ=255)
                                                                                                                                                                      ggTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCt                                                              >  1:1943663/1‑239 (MQ=255)
                                                                                                                                                                        ttAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTAc                                                            >  1:807015/1‑239 (MQ=255)
                                                                                                                                                                         tAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                         >  1:560867/1‑97 (MQ=255)
                                                                                                                                                                         tAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                           >  1:2013211/1‑239 (MQ=255)
                                                                                                                                                                           c‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCAGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                           >  2:1345957/1‑237 (MQ=255)
                                                                                                                                                                                  agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAACCTATGATGAGTTCTACAACTAGCat                                                   >  2:2196064/1‑239 (MQ=255)
                                                                                                                                                                                  agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCat                                                   >  2:2016116/1‑239 (MQ=255)
                                                                                                                                                                                       cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTa                                               >  1:120328/1‑238 (MQ=255)
                                                                                                                                                                                              gtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGccttcct                                       >  2:1191091/1‑239 (MQ=255)
                                                                                                                                                                                                 tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGt                                    >  1:1324412/1‑239 (MQ=255)
                                                                                                                                                                                                 tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGt                                    >  2:1619778/1‑239 (MQ=255)
                                                                                                                                                                                                    tgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGtttt                                 >  2:2037987/1‑239 (MQ=255)
                                                                                                                                                                                                           gATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACCCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACaa                          >  1:645782/1‑239 (MQ=255)
                                                                                                                                                                                                             tAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGt                        >  2:2088131/1‑239 (MQ=255)
                                                                                                                                                                                                                tCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACa                                                           >  1:1125737/1‑201 (MQ=255)
                                                                                                                                                                                                                   aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGatat                  >  1:411006/1‑239 (MQ=255)
                                                                                                                                                                                                                     aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTa                >  1:87242/1‑239 (MQ=255)
                                                                                                                                                                                                                     aCCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCAAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTa                >  1:1866883/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAg               >  1:1312264/1‑239 (MQ=255)
                                                                                                                                                                                                                         ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACt                                                                          >  1:479079/1‑177 (MQ=255)
                                                                                                                                                                                                                          cAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTg           >  2:546013/1‑239 (MQ=255)
                                                                                                                                                                                                                          cAGCAACCTGCATGGCAG‑‑AAATAGAGGCGATAACAGCCATGATAATGGAGATGACATCGAACGGGATGTTACCgg                                                                                                                                                                               >  1:321931/1‑75 (MQ=255)
                                                                                                                                                                                                                               aCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTAtt      >  2:406003/1‑239 (MQ=255)
                                                                                                                                                                                                                                   gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTa  >  1:53442/1‑239 (MQ=255)
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                      
TCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAA‑GATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTA  >  NC_007779/4371037‑4371502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑