Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 346,919 G→C 12.5% T250R (ACG→AGG)  prpR ← propionate catabolism operon regulatory protein PrpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779346,9190GC12.5% 226.7 / 2.4 98T250R (ACG→AGG) prpRpropionate catabolism operon regulatory protein PrpR
Reads supporting (aligned to +/- strand):  ref base G (36/48);  new base C (11/3);  total (47/51)
Fisher's exact test for biased strand distribution p-value = 1.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATAGATAAAAATTCCGGTCAT  >  NC_007779/346681‑347157
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
gCCAATCTCACCCAGAACCCGGCTCCCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCg                                                                                                                                                                                                                                                 <  1:2027653/239‑1 (MQ=255)
    aTCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAAATACC‑CATG‑AATCGCCCGGGCCGCCCGCCC‑TTTgcccgccc                                                                                                                                                                                                                                              >  2:1585351/1‑238 (MQ=255)
         aTCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTT‑ACGGCAACAAA‑CGGATGCGACTTTTTTGCCCTGTCGCGCCTCGTGGCGGGCAAAAAAATCCC‑GCTG‑AAACGCCTGGGCCGCCCGCTC‑TTTTCCCGTCCCCgct                                                                                                                                                                                                                                         >  1:26473/1‑236 (MQ=255)
          tCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑CATG‑AATCGCCCTGGCCGCCCGCCC‑CTTGCCCGTcccccgtt                                                                                                                                                                                                                                        >  2:1974588/1‑234 (MQ=255)
            cAGACACAGCGTCCCGCCGTGGGCAATTCCGAACACCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTccc                                                                                                                                                                                                                                     <  1:1939293/239‑1 (MQ=255)
            cAGAACCCGCGTCCCGCCGTGGGCAATTCCGAACAGCCCGGCGCC‑CCCTCCGCGCCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTccc                                                                                                                                                                                                                                     <  1:741772/239‑1 (MQ=255)
              gAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCCAAAAAATTCC‑GATT‑AATCGCCTGGTGCGCCCGCTC‑TTTTCCCGTCCCCCCTTcccc                                                                                                                                                                                                                                    >  2:2208360/1‑238 (MQ=255)
                   aGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GCTG‑AATCCCCTGGGCCGCCCGCTC‑TTTTACCGTCCCCGTTTCCCCCtcac                                                                                                                                                                                                                               >  1:403103/1‑237 (MQ=255)
                   aGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑CATG‑AATCGCCTGGGCCGCCAGCCC‑TTTTCCCGTCCCCGTTTCCCCCtcaa                                                                                                                                                                                                                               >  1:1381848/1‑238 (MQ=255)
                     cGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTTCC‑CGTT‑CATCGCCCGGGCCGCCAGCCC‑TTTGCCCGTTCCCGGTTccccccccaccc                                                                                                                                                                                                                            >  1:1355920/1‑232 (MQ=255)
                     cGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGGCGCCCGCTC‑TTTGCCCGTCCCCGTTTCCCCCcccatca                                                                                                                                                                                                                            >  1:1741801/1‑239 (MQ=255)
                       tACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGGAAAATATTCCC‑CATGAAATCCCCT‑GGCCCCCAGCTCTTTTCCCCGCCCCCGTTTCCCCC‑CAATCAac                                                                                                                                                                                                                          >  1:1213323/1‑239 (MQ=255)
                       tACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GATG‑AATCGCCCGGGCCGCCCGCCC‑TTTGCCCCTCCCCGTTTCCCCCCCCATCAac                                                                                                                                                                                                                          >  1:1456006/1‑239 (MQ=255)
                            ccgTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTTCC‑GATG‑AATCGCCCGGGCCGCCCGCCC‑TTTGCCCCGCCCCCTTTCCCCCCCCATCCACAccc                                                                                                                                                                                                                      >  2:417377/1‑237 (MQ=255)
                               tGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTTCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAAACAACAcccccg                                                                                                                                                                                                                   >  1:1591871/1‑238 (MQ=255)
                                gggCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCCC‑TTTGCCCGTCCCCCTTTCCCCCTCAATCAACAccgcccc                                                                                                                                                                                                                  >  1:370111/1‑236 (MQ=255)
                                      tttCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGt                                                                                                                                                                                                                                                <  1:199922/202‑1 (MQ=255)
                                      tttCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCCGGGCCGCCCGCTC‑TTTGCCCCGCCCCCTTTCCCCCCCACACAACCCCCCCCCCCTGGAg                                                                                                                                                                                                           >  2:1777751/1‑239 (MQ=255)
                                       ttCGAACCCCCCGGCGGGCCCCCCCGCGCGGCGCGCCGGTAAACGCCCCTCCCCCATAGCCAAACAGTTCTCCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTT‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAg                                                                                                                                                                                                           <  2:684738/239‑1 (MQ=255)
                                       tccGAACCGCCCGGCGCC‑CCCCCGGCTTCGCGAGCCGGTAACCGCCCCTTCCTCATAGCCAAACAGTCCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAC‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGc                                                                                                                                                                                                          <  1:1974588/237‑1 (MQ=255)
                                            accgCCCGGCGCC‑ACCCCCGCGCCGCGGGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                                                                                                                                                                                                     <  1:1779518/236‑1 (MQ=255)
                                            acacCCCGGGCCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTACTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTGGCCGGCAACAAA‑CGGATGCGAC‑TTTTTCCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGCCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCACCACCGCCGCACTGGAGCGGGCa                                                                                                                                                                                                     <  1:396111/239‑1 (MQ=255)
                                            aCAGCCAGGCGGC‑CCCTCCGCTCGGGGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                                                                                                                                                                                                     <  1:417377/239‑1 (MQ=255)
                                                cccGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAAATTCC‑GATG‑AAACGCCTGGGCCGCCAGCCC‑TTTGCCCCCCCCCCGTTCCCCCCCAATCAACCCCGCCGCACCGGCCCGGGCCTAc                                                                                                                                                                                                  >  2:2129570/1‑238 (MQ=255)
                                                 ccGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCCGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCCATCAACACCGCCCCAcccgg                                                                                                                                                                                                            >  1:1329520/1‑223 (MQ=255)
                                                 ccGGCGCA‑CCCTCCGGGCAGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAACCAGTTCTGCGTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAg                                                                                                                                                                                                <  1:531502/239‑1 (MQ=255)
                                                   ggCGCC‑ACCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCa                                                                                                                                                                                              <  1:1585351/239‑1 (MQ=255)
                                                         caCCCCCGCGTGGCAGGCCGGTAAACGCCCCTTCCCCATAGCCAAACAGTTCTGCTTCCAGCCGCGATTCGGCAATCGCCCCGCAGTGGACGGAAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATA‑AACCGCCTGGGCCGCCAGTTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATa                                                                                                                                                                                         <  2:100629/238‑1 (MQ=255)
                                                           ccccccgtccccgAGCCGTTAACCGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCATTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑t                                                                                                                                                                                      <  1:2085963/228‑1 (MQ=255)
                                                           cccccGCGTCGGGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑t                                                                                                                                                                                      <  1:1756037/236‑1 (MQ=255)
                                                           cccccGCGGCGCGAGCCGGTAAACCCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATa                                                                                                                                                                                         <  1:545285/234‑1 (MQ=255)
                                                           ccTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCCCAATCAACACCGCCGCACTGGAGCGGGCCAACAGCaaaaaaa                                                                                                                                                                                        >  1:354185/1‑235 (MQ=255)
                                                               cgcgTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCCC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACCCCCCCCCCCTGGAGCGGGCATACAGCaaaaaccgtct                                                                                                                                                                                    >  1:1043097/1‑231 (MQ=255)
                                                                     gcgAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AAACCCCCGGGCCGCCCGCTC‑TTTTCCCCGCCCCCGTTCCCCCCCAATCAACACCCCCCCCCTGGAGGGGGGATACCACAAAAAAA‑GCTGCCGGAc                                                                                                                                                                             >  2:545285/1‑238 (MQ=255)
                                                                           cGGTAAACGCCCTTTCCTCATAGCAAACCAGTTCTCCTCCCAGCAGCTCTTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑GGGATGCGAT‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATCCCC‑GATG‑AACCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTcc                                                                                                                                                                      <  2:1381848/239‑1 (MQ=255)
                                                                              tAACCGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTAc                                                                                                                                                                             <  1:622343/229‑1 (MQ=255)
                                                                              tAAACGCCCCTTCCTTATAGCCAAACAGTTCTTCTTCCAGCAGCGATTCGGCAATCCCCCCGCAGTTGACGGCAACAAC‑CGGATGCGAC‑TTTTTGCCCTGCCGCGCATCGTGGCGGGCAAAATTTTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCAt                                                                                                                                                                    <  1:1860890/238‑1 (MQ=255)
                                                                              tAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATCCCC‑GATG‑AATCGCCGGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCAt                                                                                                                                                                    <  1:503922/238‑1 (MQ=255)
                                                                              tAAACGCCCCTTCCTCATACCCAAACAGTTCGGCTTCCAGCAGCTCTGCACGATCCGCCCCGCCGTCGAGGGCAAAAAA‑CGGACGGGAA‑TTGTGACCCTGCCGATCATCGGGGCGGAAAAAAAATCCCC‑GATA‑AACCGCCGGGGCCCCCAGCTT‑TTTGCCGGTCCCCGTTCCCCCCCCAAACAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCAt                                                                                                                                                                    <  2:1741801/238‑1 (MQ=255)
                                                                                       cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTTGGCCGCCAGCTC‑TTTTCCCGTCCCCCTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAC‑TCCTCCGTACTTGTTCCATCTGGGGTGAt                                                                                                                                                          >  1:1992945/1‑239 (MQ=255)
                                                                                       cTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCa                                                                                                                                                                                                     <  1:1658511/197‑1 (MQ=255)
                                                                                        ttCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGTTTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GGTT‑AAACTCCTTGGCCGCCCGCTC‑CTTGCCCGCCCCCCTTTCCCCCTCAATCAACACCGCCCCCCAGGGGCGGGCACACCACAAAAAAG‑TCTCACACACTTTTTCCacc                                                                                                                                                                   >  2:622343/1‑227 (MQ=255)
                                                                                            tCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGAcc                                                                                                                                                     <  1:504942/239‑1 (MQ=255)
                                                                                            ccATCGCCAAACAGTTCTGCTCCGAGCAGCGATTCGGCAATCGCCCCGCAGTGGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCAGCGTGGCGGGCAAAATATCCCC‑GATG‑AATCGCCTGGGCCGCCAGCTT‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGAcc                                                                                                                                                     <  2:488385/238‑1 (MQ=255)
                                                                                                 gCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTTCCCCCCCCCCTTTCCCCCCCAATCCACCCCCCCCCACTGGAGCGGGCATACCGCcaaa                                                                                                                                                                                           >  2:1658511/1‑197 (MQ=255)
                                                                                                     aaCAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCatat                                                                                                                                             >  1:424982/1‑238 (MQ=255)
                                                                                                     aaCAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCCC‑TTTGCCCGTCCCCGTTTCCCCCTCACTAAACACCGCCGCACCGGAGCGGGCATACAGCACAATAGATCT‑CCGCACCTGGTCTCTCTGTGGTGATTGTCCCGGCATATc                                                                                                                                            >  2:1218121/1‑239 (MQ=255)
                                                                                                          ttCTGCCTCCAGCACGGATTCGGCAATCCCCCCGCGGTTGACGGCAAAAAA‑CGGATGCGAT‑TTTTTGCCTTGTCGCCCATCGGGGCGGGAAAAATATTCCC‑GATG‑AACCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGccc                                                                                                                                        <  2:1403022/238‑1 (MQ=255)
                                                                                                            cTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATAATCCC‑GATT‑AATCCCCCGGGCCGCCACCTC‑CTTTCCCGTCCCCGTTTCCCCCCCAAACAACACCCCCGCCACGGAGCGGGCCTCCCGCAAAATAG‑ACCGCCGGACTCTTTCCATCCGGGGGGATTGCACGAAGATAACGccccg                                                                                                                                      >  2:1595039/1‑236 (MQ=255)
                                                                                                                          gATTCGGCATCCCCCCCGCAGTTGACGGCAAAAAC‑CGGTTGCGAC‑TTTTGCCCCTGTCCCGACTCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCTCCTT‑TTTGCCCGCCCCCGTTCCCCCCCCAACCACCACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACg                                                                                                                       <  2:837026/239‑1 (MQ=255)
                                                                                                                           atccGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGTTGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGCCAAAATATTCCC‑GATG‑AAGCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGc                                                                                                                      <  1:2129570/236‑1 (MQ=255)
                                                                                                                           aTTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATAGCCCGGGCCGCCAGCCC‑TTTTTCCCTCCCCCTTTCCCCCCCAATCAACACCCCCCCCCTGGAGCGGGCCAACAGCAAAAAAG‑GCCGCCGTACTTGTTTCATCTGTGGGTATTGACCGAGCATAACGCCCAACACGTAACGAGTACGc                                                                                                                      >  2:665694/1‑239 (MQ=255)
                                                                                                                           aTTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GATG‑AATCGCCTGGGCCGCCCGCTC‑TTTGCCCCTCCCCCTTTCCCCCCCCATTAACACCCCCCCACCGGGGCGGGCCAAAAGCAAAATAA‑TCTTCCGTACATGTTCCCTCTGTGGGGGTTGACCGAGACAAACGCCCACCCACTAACGAGTAcc                                                                                                                       >  2:2016133/1‑237 (MQ=255)
                                                                                                                           aTTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCCC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCCCACCGGAGCGGGCATACAGCAAAATAG‑TCTGCCGCACTTGTTCCCTCTGTGGTGATTGACCGAGCATATCGCCCCCCACGTAACGGGTACg                                                                                                                       >  2:808219/1‑238 (MQ=255)
                                                                                                                             tCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCTTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑Tc                                                                                                                                                                                     <  1:955354/174‑1 (MQ=255)
                                                                                                                             tCGGCAATCGCCCCGCAGTTGACGGAAACAAA‑CGGATGCGCC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCGGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACa                                                                                                                                                                                                 <  1:785332/163‑1 (MQ=255)
                                                                                                                                   aTCCCCCCGCGGTTGACGGCAAAAAG‑CGGATGCGTC‑TTTTTCCCCTGCCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AACCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACCCCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGtt                                                                                                              <  2:944076/239‑1 (MQ=255)
                                                                                                                                       ccccGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCCAAAAATTCCC‑GATG‑AATCGCCTGGGCCGCCCGCTC‑TTTGCCCGTCCCCCTTTCCCCCCCAACCAACACCCCCGCCCTGGGGCGGGCCCACAGCAAAATAG‑t                                                                                                                                                                                      >  2:785332/1‑163 (MQ=255)
                                                                                                                                           gCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑ACTCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTgg                                                                                                      <  1:398864/239‑1 (MQ=255)
                                                                                                                                                 gACGGCAACAAA‑CGGATGCCAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGCCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACTCCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGt                                                                                                <  1:1701679/239‑1 (MQ=255)
                                                                                                                                                   acggaacaaACCGGAGGCGAC‑TTTTGCCCCGGTCGCGCTTGGTGGCGGGAAAAATATTCCC‑GATG‑ACTCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGt                                                                                                <  2:354185/234‑1 (MQ=255)
                                                                                                                                                    ggaaacaaA‑GGGATGCGAC‑TTTCTGCCCTGGCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑ACTCGCCTGGGCCGCCATCTC‑TTTGCCCGTCCCCGTTTCCCCCGCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGa                                                                                              <  1:2016133/235‑1 (MQ=255)
                                                                                                                                                    ggCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCCC‑TTTGCCCGTCCCCGTTTCCCCCTCACTCAACACCCCCGCACCCGGGCGGGCCTACAGCAAAATAC‑TCTGCCGTACCTGTTCCCTCTGTGGTGGTTGACCGCGCATATCCCCCAGCACGTAACGAGTACCCAGGGCGTTTCGggcggcccccggag                                                                                             >  2:1739754/1‑231 (MQ=255)
                                                                                                                                                      caacaaA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTTCC‑GATG‑AATCGCCTGGGCCGCCCGCCC‑TTTGCCCGCCCCCCTTTCCCCCCCCATCAACACCCCCCCACCGGAGCGGGCCCACAGCCAAATAA‑TCCTCCGCACCTGTTCCATCCGTGGGGGTTTACCGAGCATAACGCCCAGCACCTAAACAGTACCCCGGGCGGTGCGGGTGGCATCGCTAgtg                                                                                           >  2:281998/1‑239 (MQ=255)
                                                                                                                                                      caacaaA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTTCC‑GATG‑AATAGCCTGGGCCGCCCGCTC‑TTTGCCCCTCCCCCCTTCCCCCCCAATCAAAACCCCCGCCCCGGGGCGGGGCTACAGCAAAATAA‑GCCTCCGGACTTGTTCCAACTGGGGTGGTTTAACGAGCAAATCTCCCCGCACCGAAAAGGTACGCAGGGCGGTGCGggcggcggcggggggg                                                                                           >  2:582881/1‑229 (MQ=255)
                                                                                                                                                            accGGATGCGAC‑TTTTTCCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCACCACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTAc                                                                                                                                                                             <  1:2066186/150‑1 (MQ=255)
                                                                                                                                                              cGGAGGCGCC‑TTTTTGCCCGGCCGCGCATCGTGGCGGGCAAAATTTTCCC‑GATG‑AATCGCCTGGGCCCCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGc                                                                                    <  1:1248254/239‑1 (MQ=255)
                                                                                                                                                                 aTGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GATG‑AATCGCCTGGGCCGCCCGCTC‑TTTGCCCGTCCCCGTTTTCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAAAAG‑TCTTCCGTACTTTTTCCATCTGTGGGGATTGAACGAGCATAACGCCCAGCACGTCACGAGTACGCAGGGGGTTGCGGGTGGCATCGGGAGTGGTCTggggga                                                                                 >  2:1248254/1‑235 (MQ=255)
                                                                                                                                                                  tGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAAAATTCCC‑GCTG‑AATCGCCTGGGCCGCCAGCTC‑TTTTCCCGTCCCCGTTTCCCCCTCAATCAACACCCCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTTCCGTACTTGTTCCATCCGCGGTGGATGACCGAGCATAACCCCCACCACGCAAACAGGACGCCGGGCGTTGCGGGTGGCATCGTGGGTGTTATGGCGTaa                                                                                >  2:1101542/1‑239 (MQ=255)
                                                                                                                                                                      aC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTTCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTTCCCGGCCCCCTTTCCCCCTCAATCAACACCGCCCCCCTGGGGCGGGCATACCGCAAAATAG‑TCTGCCGTACTTGTTCCCTc                                                                                                                                                                   >  2:2066186/1‑152 (MQ=255)
                                                                                                                                                                      aC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATAATTCC‑GATG‑AATCGCCCGGGCCGCCCGCCC‑TTTTCCCGTCCCCGTTTCCCCCTCCATCCAAACCGCCGCACTCGGGCGGGCCTAACGGAAAATAC‑TCCGCCGTACTTGTTCCATCTGTGGTGGTTGACCTAGCACATCCCCCCGCCCGTAACGAATACGCAGGGCGTTGCGGGTGGCACACTGAGTGTTTTGGCGTAACGAc                                                                            >  2:1402769/1‑239 (MQ=255)
                                                                                                                                                                          ttttGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCTTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTTTTCCATCTGTGGTGATTGACCGAGCATATCGcc                                                                                                                                         <  1:1632230/175‑1 (MQ=255)
                                                                                                                                                                           tttGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATgc                                                                        <  1:1777751/239‑1 (MQ=255)
                                                                                                                                                                           tttGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AACCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATgc                                                                        <  1:1098103/239‑1 (MQ=255)
                                                                                                                                                                           ttggCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATgc                                                                        <  1:945350/236‑1 (MQ=255)
                                                                                                                                                                                ccTTTCGCGCATCGTGCGGGGCAAAATATTCCC‑GATG‑AACCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCAGCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTc                                                                   <  1:281998/239‑1 (MQ=255)
                                                                                                                                                                                  tGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GCTG‑AATTGCCTTGGCCGCCAGCTC‑TTTGCCCCTCCCCGCTTCCCCCTCCATCCACACCGCCCCACTGGAGCGGGCATACAGCAAAATAA‑TCTGCCGTACTTGTTCCCTCTGTGGGGATTGACCGCGCATATCCCCCAGCACGTAACGAGTACGCAGGGCGGTGCGGGGGGGATCGTGAGTGTTATGGCGGAACGACCTTCGCGTcct                                                                 >  2:2134381/1‑237 (MQ=255)
                                                                                                                                                                                   gTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTTGGCCGCCAGCTC‑TTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCata                                                                >  1:1696941/1‑239 (MQ=255)
                                                                                                                                                                                            cGTGGCGGGCAAAATATTCCC‑GATT‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCCTCCCCTTTCCCCCCCCAACCACCTCCTCCGCACTGGATCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCa                                                       <  1:2008757/239‑1 (MQ=255)
                                                                                                                                                                                              tGGCGGGCAAAATATTTCC‑GATG‑AAACGCCTGGGCCGCCCGCTC‑TTTGCCCGTCCCCGTTTCCCCCCCCATCAACACCCCCCCACTGGGGCGGGCCTACAGCCAAAAAA‑GCTGCCGGACTTGTTCCATCTGTGGGGGTTTACCGGGCATAAAGCCCAGCACGTAACGAGTACGCAGGGCGGTGGGGGGGGGATCGTGGGGGGTTAGGCGGAAAGACATGCGCGTCATATACacggcac                                                      >  2:162371/1‑232 (MQ=255)
                                                                                                                                                                                              tGGCGGGCAAAATATTCCC‑TATG‑AATCCCCTGGGCTGCCCGCTC‑CTTTCCCCTCCCCCTTTTCCCCCCCATCCACACCCCCCCACTGGAGCGGGCCCCCAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTTTGGTGGTTGACCCAGCCTAACGCCCCGCACGTAACCAGTACGCCGGGGGTTGCCGGGGGTCTCGCTGGGGGTATGGCGTAACGGCCTGCGCGCCCTATCCacgccat                                                      >  2:654804/1‑232 (MQ=255)
                                                                                                                                                                                               gagcggCAAAAATTTCCCGGATG‑AACCGCCGGGGCCGCCAGCTC‑TTTGCCCGCCCCCGTTTCCCCCCCAACCACCACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCAt                                                      <  2:1829462/234‑1 (MQ=255)
                                                                                                                                                                                                   ggCAAAATATTCCC‑GATG‑AAACGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAAAAG‑TCCGCCGTACTTGTTCCATCTGTGGGGATTGACCGAGCATATCCCCCCGCACGTAACCAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGCGTTATGGCGGAACGACCAGCGCGCCATATCCAGCGCATCGCTgt                                                >  2:1979779/1‑238 (MQ=255)
                                                                                                                                                                                                    gCAAACTATTCCC‑GAGG‑ACCCCCCGGGCCCGCCAGCTT‑TTGACCGGTCCCTCTTCCCCCCCCATACACCTCCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGa                                                <  1:884226/238‑1 (MQ=255)
                                                                                                                                                                                                             tCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGgc                                       >  1:85947/1‑238 (MQ=255)
                                                                                                                                                                                                                   aTG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACgg                                 >  2:1803219/1‑239 (MQ=255)
                                                                                                                                                                                                                   aTG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACgg                                 >  2:553432/1‑239 (MQ=255)
                                                                                                                                                                                                                    tG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGt                                >  1:543807/1‑239 (MQ=255)
                                                                                                                                                                                                                    tG‑AATCGCCTGGGCCCCCAGCTC‑TTTGCCCGTCCCCGTTCCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGt                                <  1:162371/239‑1 (MQ=255)
                                                                                                                                                                                                                     g‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTg                               >  1:2210872/1‑239 (MQ=255)
                                                                                                                                                                                                                     g‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTg                               >  1:315572/1‑239 (MQ=255)
                                                                                                                                                                                                                             cTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGcggcgg                        <  1:896414/239‑1 (MQ=255)
                                                                                                                                                                                                                              tGGCCCTCCAGCTC‑TTTGCCCGTCCCCGTTCCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTGCTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGa                       <  1:981946/239‑1 (MQ=255)
                                                                                                                                                                                                                               gggCCGCCAGCTC‑TTTGCCCGTCCCTGTTGCCCCCTCAATCACCACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGaa                      <  1:1101542/239‑1 (MQ=255)
                                                                                                                                                                                                                               gggCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGaa                      <  1:934293/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgccAGCTC‑TTTGCCCGTCCCCTTTCCCCCCTCAATCATCACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATa                    <  1:1402769/238‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgccAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATa                    <  2:543807/238‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgccAGCTC‑TTGGCCCCTCCCCGTTCCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCGGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATa                    <  1:1739754/238‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCCCCACCCCGTTACCCCCCCAACAAAGCCCGCCGCACGGGAGCGGGCATACAGCCAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCTAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACTACATGCGCGTCATATCCAGCGCATCGCTGAACGCCAGGCGCACGGTGGCGGCGGAATAGATAAAAATTCCg       <  2:697871/239‑1 (MQ=255)
                                                                                                                                                                                                                                                    gTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATAGATAAAAATTCCGGTCAt  >  1:1798960/1‑239 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
GCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCG‑ACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAA‑CGGATGCGAC‑TTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCC‑GATG‑AATCGCCTGGGCCGCCAGCTC‑TTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAG‑TCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAACGACATGCGCGTCATATCCAGCGCATCGCTGAACGCCTGGCGCACGGTGGCGGCGGAATAGATAAAAATTCCGGTCAT  >  NC_007779/346681‑347157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑