Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 3,545,460 C→G 22.4% V382V (GTC→GTG)  Y75_RS17610 → PTS fructose‑like transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077793,545,4600CG22.4% 97.8 / 6.3 40V382V (GTC→GTG) Y75_RS17610PTS fructose‑like transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base C (16/14);  new base G (3/6);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGTTTCTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACATTTCATTCATTAT  >  NC_007779/3545234‑3545696
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
cAGCGTTTCTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGGCTTGGGG‑CGGTCA‑TTTCGCCCCAACTGTTTTCCCCCCAGGGAAAAAAGGCGGGCAGAGGGGCCCTTGTTGTTGGGGCcccacc                                                                                                                                                                                                                                     >  2:349538/1‑236 (MQ=255)
        cTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCaacc‑ga                                                                                                                                                                                                                           <  1:721396/239‑1 (MQ=255)
        cTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCaacc‑ga                                                                                                                                                                                                                           <  1:1757724/239‑1 (MQ=255)
        cTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCaacc‑ga                                                                                                                                                                                                                           <  1:282829/239‑1 (MQ=255)
                tGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCC‑TTGCGCCCCAACCGCTTACCCGACCGGGAAAAGAGGCTGCCCCTAGTGCCCTTTTGGTGGGTGCCCCCGTGGCAACC‑CACCCGGc                                                                                                                                                                                                                     >  2:762579/1‑237 (MQ=255)
                ggctggcttGGCGTTCATTAGGGGCGCCCCAATAAATAAACCCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCt                                                                                                                                                                                                                    <  2:1447851/230‑1 (MQ=255)
                  ggattcgtgtgTTCGATAGGGCCGGCCCCATCAATAAACCCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTAt                                                                                                                                                                                                                  <  2:1926482/228‑1 (MQ=255)
                  ctgtGCTGGCTGTCGATTTGGGCGGCCCCCACAAAAAACCCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTAt                                                                                                                                                                                                                  <  2:1580706/235‑1 (MQ=255)
                     tGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGGCTTGCGC‑CGGTCATTTCCCCCCAACCCTTTTCCCCCACGGGAAAAA‑AGGCGGCCCGTAGGGCCCCTTTGGGCGGTGGCCGCCTCGCCACC‑CAACCGGcccattct                                                                                                                                                                                                              >  2:2227827/1‑232 (MQ=255)
                          gCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGGCTTCCTACCGTCCT‑TTGC‑CCCCCACCGTTTTACCGCCCCGACAAAAAGGGCGCCACTAGTGCCCTCCTGGTGGGTGCCGCCGACGCAAAC‑CCACCCCCTATTCCtcatg                                                                                                                                                                                                          >  2:1614255/1‑234 (MQ=255)
                                gATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGc                                                                                                                                                                                                   <  1:1241140/239‑1 (MQ=255)
                                            ccaaTCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGcc                                                                                                                                                                                       <  1:328981/236‑1 (MQ=255)
                                             ccATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCt                                                                                                                                                                                                                    <  1:1797013/209‑1 (MQ=255)
                                                            ccTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTTCGA‑CGGTCA‑TTGCGCCCAAACTGTTTACCCGCCAGGGAAAAGAGGCTGCCCGTAGTGCCCATGTGGGCGGGGCCCCAGGCGCAACC‑AGACCCGCCATTCCTTTCCCTCTGGCTCGCCCCTTGCCGGTGGTTACCCCCaac                                                                                                                                                                       >  2:1946421/1‑238 (MQ=255)
                                                            ccTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGGCTTCCGA‑CCGTCT‑TTGCGCGCAAAATGTTTACCCCACCGGCAAAAGAAGCCGGCAGTAGTTCCATTGTGGTGGGGGCCCCACTCGCTACCTAAACCCCCTGTCTCTTAC‑CCCTGGCAGCCCCCTTGCCGCAGATTTACGcccaa                                                                                                                                                                       >  2:1124789/1‑236 (MQ=255)
                                                                 ttcttcGGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTCCGCCATCGTCGGCGTTGTCGCGCTGATGCCTCCCGTCGCGGCGGGTCTTGCGA‑GGTTCA‑TTGCGCCCAAACTGTTTCCCCGACAGGAAAAAGAGGCTGCCAGTAGTCCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCg                                                                                                                                                                                      <  1:1806513/219‑1 (MQ=255)
                                                                                            cacaTTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCaacc‑ga                                                                                                                                                                                                                           <  1:1314068/155‑1 (MQ=255)
                                                                                                                               cggggggctGCCGCCGGCCGGGGGGGTTGTTGGGC‑CGTTCA‑TGGCCCCCAACTTGTTCCCCGGCAAGAAAAAAGGGGCGGCCGGTAGTGCATTGGTGGTGGGTCCCACAGGACCACCC‑GACCCGGTTATTCCTTCCTCTCGGGCGTCTCCTTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTCCCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGt                                                                                                     <  2:2015243/230‑1 (MQ=255)
                                                                                                                               cccggtGATGCCCCCCGGCGCGGGGGGTTTGGCGC‑GTTTCT‑TCGCCCCCAATTTTTTACCCCGACAGGAAAAAGGGGCTGCCGGTGGTGCCATTGTGGTCGGCCCCAGAGTCGCACCC‑GACCCGGCTATTCCTTCCGCGCGGGCAGCGCCGTTGCCGTTGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGt                                                                                                     <  2:956310/233‑1 (MQ=255)
                                                                                                                                  gcTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTGCGCTCTGGCAGCTCCGTTGcc                                                                                                                                                                                       <  1:2089451/153‑1 (MQ=255)
                                                                                                                                                                 gcgtttCA‑TTGCGCCCAATCTGTTTCCCCGACAGGAAAAAGAGGCTGCCAGTGGTGCCATTGGGGTGGGTGCCACAGTCGCAACC‑GACCCGGCTATTCCTTCCGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATg                                                                    <  1:38909/234‑1 (MQ=255)
                                                                                                                                                                              gcCCAACCTGTTACCCCGACAGGAAAAAGGGGCTGCGAGTAGTGCCATTGGGGTGGGTGCCACAGTCGCAACC‑GACCCGGCTATTCCTTACGCTCGGGCGGTTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGt                                                        <  1:2227827/238‑1 (MQ=255)
                                                                                                                                                                                                aCAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGGGATCGCTTTCGGAATCAAACGTCCGGCACCTGGGTTGGGGATCTTTTACCCGCTGATCGGCCTGATGTCGCCGGGGGGGTC‑GTTTTTTCTGGTGCTg                                     >  1:1327547/1‑238 (MQ=255)
                                                                                                                                                                                                aCAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGCCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTTGGAATCAAACGGCTGGCCCCCGGTTTTGGTGTCTTTGTCCCCCTGATCGGCCCGATGTCCCCGGTGGGTTC‑GTTTTAttttggtcctg                                    >  2:682767/1‑229 (MQ=255)
                                                                                                                                                                                                      aaaaGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGGGCCGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGGATCTTTGACCCGCTGATCGGCCCGATGTCGCCGGTGGGTTC‑GTTTTATCTGGCGCTGGCGATTg                              >  1:1427240/1‑239 (MQ=255)
                                                                                                                                                                                                            ggCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGGTCGCCTTCGGCATCAAACGTCCGGCACCCGGTTTGGGGGTTTTTTAACCCCCGGTCGGCCTGGTGTCCCCGGGGGGTGG‑GTTTTTTTTTGTGGTTGCGGGTGGcccc                         >  2:735674/1‑236 (MQ=255)
                                                                                                                                                                                                             gCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCga                                                                                                                                                                                     <  2:1220046/82‑1 (MQ=255)
                                                                                                                                                                                                             gCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCga                                                                                                                                                                                     >  1:1220046/1‑82 (MQ=255)
                                                                                                                                                                                                              cTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCCTTACCCGCGGGCTGGGGATCGCCTTCCGGAACCAACGGCTGGCACCTGGCTTGGGGATCTTTTGCCCGCTGATCGGCCTGGTTTCGCCGGGGGGGTCGGTTTTTTCTGG‑GGTGGCGATTGGCCTcgc                       >  2:476809/1‑238 (MQ=255)
                                                                                                                                                                                                                        ttccATTGTGGTGGGCGCCACAGTCGCAACC‑GACCCGGCTTTTCCTTCCGCTCTGGCAGCTCCGTTGCGGATGATTCCCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACAtt             <  1:1946421/236‑1 (MQ=255)
                                                                                                                                                                                                                        ggCCATGGGGGTGGGTGCCACGGTCGCAACC‑GAACCGGTTATTCCTTCCGCTCGGGCAGCTCCGTTGCCGATGTTTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACAtt             <  1:81681/237‑1 (MQ=255)
                                                                                                                                                                                                                        gcccttgggggtgggtgCCACGGTCGCAACC‑GACCCGGTTATTCTTTACGCGCGGGAAGGTCGGGTGCGGATGTTTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACAtt             <  1:179192/229‑1 (MQ=255)
                                                                                                                                                                                                                            aTTGTGGTGGGTGCCCCGGTCCCAACC‑CAACCGGCTTTTCCTTCCGCTCTGGCGGCGCGTTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACATttcatt        <  1:1216861/239‑1 (MQ=255)
                                                                                                                                                                                                                               gtgGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTTCCGGCGCGCTGGGGATCCCGTTTGGGATCAAACGGCTGGGCCCTGGTGTGGGGATCTTTGACCCGCCGATCGGGCTGATGTGCCCGGGGGGTGT‑GGTTTTTCTCGGGCCTGCGGATGGGCTCCCGCGGGACATTtctttaat     >  2:260523/1‑239 (MQ=255)
                                                                                                                                                                                                                               gtgGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCTGGAACAAACGTCTGGCACCTGGTTTGGGGATCTTTTACCCGCTGATCGGCCTGATGTCGCCGGGGGGGTC‑GTTTTTTTTGGTGCTGGCGATTGTCCGCGCCCTTGTCATttcattcct     >  2:1700350/1‑237 (MQ=255)
                                                                                                                                                                                                                               gtgGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGGGATCGCCTTCGGGATCAAACGGCCGGCCCCTGGGTTGGGTATCTTTTACCCGCCGGTCAGGCTGATGTCGCCCGTGGGGTC‑GGTTTTTTTGGGGGTGGCGGTTGGCCCCCCGCGTGACATTttcttcat     >  2:918709/1‑239 (MQ=255)
                                                                                                                                                                                                                               gtgGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCCGCGGGCTGGTGATCGCCTTCGGGATCAAACGTCTGGCACCCGGTTTTGGGGTCTTTGACCCCCTGATCGGCCTGATGTCGCCGGTGGGGGC‑GTTTTTTCTGGTGCTGGCGATTGGCCTCGCGCTGAACCTTtcatttat     >  2:809395/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGGTTGGGTATCTTTGACCCGCt                                                                                 >  1:1242232/1‑161 (MQ=255)
                                                                                                                                                                                                                                  gTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCCGGTTTGGGTATCTTTTACCCGCCGATCGGCCCGATGTCGCCGGGGGGTTT‑GTTTTATCTGGTGCTTGCGATTGGCCTCGCGCTGAAACTTTTATTCCTTAt  >  2:1957625/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCCTTCGGAATCAAACGTCTTGCACCTGGTTTGGGGTTCTTTTACCCGCCGATCGGCCTGATGTCGCCGGGGGGGTC‑GTTTTTTCTGGGGCTGGGGGTTTGGCCCCCGCTGGACAtttttttttttat  >  2:425663/1‑229 (MQ=255)
                                                                                                                                                                                                                                  gTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGGGATCGCTTTCGGAATCCAACGTCTGGCACCTGGTTTGGGTATCTTTTACCCGCTGATCGGGCTGATTGCGCCGGTGGGGTC‑GGTTTTTTTGGTGCTGGGGATTTGCCTCGCGCTGGAACTTTcattcatttt  >  2:1935289/1‑237 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              
CAGCGTTTCTGGTCGGTGCGATGCTGGCGTTCGATATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCATCGTCGGCGTTGTCGCGCTGATGCCGCCCGTCGCGGCGGGTCTTGCGA‑CGTTCA‑TTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTGCCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACC‑GAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATGATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTTGGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTGGGTTC‑GTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGAACATTTCATTCATTAT  >  NC_007779/3545234‑3545696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑