Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 4,104,263 A→C 11.1% K144N (AAA→AAC)  ompR → two‑component system response regulator OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,104,2630AC11.1% 233.4 / 2.9 99K144N (AAA→AAC) ompRtwo‑component system response regulator OmpR
Reads supporting (aligned to +/- strand):  ref base A (47/39);  new base C (2/10);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 2.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TTTGCCGACGTCTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAG  >  NC_007779/4104028‑4104500
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
tttGCCGACGTCTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACtt                                                                                                                                                                                                                                                 <  1:2228678/239‑1 (MQ=255)
tttGCCGACGTCTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACt                                                                                                                                                                                                                                                  <  1:593745/238‑1 (MQ=255)
                 aGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAc‑g‑cgcg                                                                                                                                                                                                                               <  1:293725/238‑1 (MQ=255)
                   tCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTTCTTTC‑GGGAAG‑TTCA‑AACTTAACCTCGGGAC‑G‑CGCGaaa                                                                                                                                                                                                                            >  1:1649167/1‑239 (MQ=255)
                   tCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑CTGCATTC‑GGTAAG‑GTCC‑AACTTAACCCCGGTAC‑G‑CGCGaaa                                                                                                                                                                                                                            >  1:2168683/1‑239 (MQ=255)
                    cAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGaa                                                                                                                                                                                                                             <  1:904356/237‑1 (MQ=255)
                       agCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑GTTCTTTC‑CGGAAG‑TTTA‑AAACTAACACCGGGAC‑G‑CGCGGAATggt                                                                                                                                                                                                                        >  2:770245/1‑237 (MQ=255)
                       agCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑GAA‑TTGCTTTCGGGGAAG‑TCCA‑AA‑TTAAACCCGGTAC‑G‑CGGGaaaagg                                                                                                                                                                                                                         >  1:594674/1‑235 (MQ=255)
                        gCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGtt                                                                                                                                                                                                                        <  2:2151666/238‑1 (MQ=255)
                            cccGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAc‑g‑c                                                                                                                                                                                                                                  <  1:1860315/224‑1 (MQ=255)
                                 tGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGCTAA‑TACCTTTC‑GGCAGT‑TTCA‑AAC‑TAACCCCGGGAC‑G‑CGGGAAAAGtttctcgcaacc                                                                                                                                                                                                              >  2:2258142/1‑229 (MQ=255)
                                      aTCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTTGTTTT‑GGTAAG‑TTCA‑AAATTTACCCCGGTAC‑G‑CGCGGAAAGtt                                                                                                                                                                                                                        >  2:1860315/1‑224 (MQ=255)
                                      aTCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑CAA‑AGTCTTTC‑GCTAAA‑TTCACAACTTAACC‑CGGTAC‑C‑CGCGCAATGTGCcgctgaaacgcgc                                                                                                                                                                                                          >  2:2142459/1‑228 (MQ=255)
                                                tGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTCAG‑TTTC‑AACTTAACCTCTGGAC‑G‑CGCGAAATGTTCCGCGCCGACGAGACGAT‑TCCGCt                                                                                                                                                                                               >  1:1781369/1‑238 (MQ=255)
                                                tGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑CAA‑TTGCTTTC‑GGTCAG‑TTCC‑AACTTAACCTCGGGAC‑C‑CGCGGCATGTTCCGCGAAGAACAGCCCAT‑GCCGcc                                                                                                                                                                                               >  1:1640504/1‑237 (MQ=255)
                                                  aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTTCTTTC‑GGGAAG‑TTCA‑AACCTAACCTCGGTAC‑G‑CGCGGAAAGGTCCGCGGAGACGAGACGAT‑GCCGCTCa                                                                                                                                                                                             >  1:1996100/1‑238 (MQ=255)
                                                  aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCCCGGTCC‑G‑CGCGAAATGGTCCGCGACGAAGCGCCGAT‑GCCGCCCa                                                                                                                                                                                             >  1:1185905/1‑238 (MQ=255)
                                                  aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGGAAG‑TTCA‑AACTTAACCCCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAACCGAT‑GCCGCTCa                                                                                                                                                                                             >  1:31160/1‑238 (MQ=255)
                                                  aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑GAATTTGCTTCC‑GTAAGG‑TTCA‑ACCTAAACC‑CGGTACGG‑CGCG‑AATGTTCCGCGGAGACGGGCCGAG‑Gccccccc                                                                                                                                                                                             >  2:745362/1‑233 (MQ=255)
                                                  aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGACTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TGA‑TTGCTTTC‑GGGAAG‑GTCA‑AACTTAACCTCGGTAC‑G‑CGCCAAATGTTCCGCGAAGACGCGCCGAC‑GCCGCTCa                                                                                                                                                                                             >  1:1980704/1‑238 (MQ=255)
                                                      ggAAAGGGGAAGAAGTGGACCGTTTCGGGGGCCTGGAGATTGGCGCTAACAACTACATTCCAAACCCGTTTAACCCGCGTGACCTGCGGCCCCGTATCCGTGGGGTGCCGCGTCGTCAGGCGAACGACCTGCCGGGCGAACCGGCACGGGAAGAGGCGG‑TAA‑TTGTTTCG‑GGTAGG‑TCCA‑ACCTTACCCTCGGTAG‑G‑CGCGAATTGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAg                                                                                                                                                                                         <  2:425668/237‑1 (MQ=255)
                                                        aaaGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTTCTTTC‑GGTAAG‑TTCA‑AACTTAACCGCGGTAA‑G‑CGCGCAAAGGTCCGCGAAGACGAGCCCAG‑GCCGACCACCACCgg                                                                                                                                                                                      >  1:1495446/1‑239 (MQ=255)
                                                         agggggAAGGATGGGACCGTTTCGTAGGCCGGGAGATTGGCTCTGGCGACTACATTCCAACACCGTTTAACCCGCGGCAACTGCTGGCCCGTACCCGGGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGGGGGGG‑TAT‑TTCTTTTC‑GGTAAG‑TAAA‑AACTTACCCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCgg                                                                                                                                                                                      <  2:1601547/236‑1 (MQ=255)
                                                                agaggtgccccGTTTCGTGGGCGGGGAGGTTGGCGCTGCCGACTCCATTCCAAAACCTTTTACCCCGCGTGAACTGCTGGCCCGTATCCGGGCGGTGCGGCGTCGTCAGGCGAACGAACTGCCGGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTTCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  2:1781369/230‑1 (MQ=255)
                                                                agaagTGGACCGTATGGGAGGCCTGGAGATGGGCCCTGACGGCTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCGGTACCCGTGGGGTGCGGCGGCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGGGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  2:1844025/239‑1 (MQ=255)
                                                                agaagTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  1:34220/239‑1 (MQ=255)
                                                                agaagGGGCCCGTATCGTAGGCCGGGGGTTGGGGGCGAGCAACTCCATTCCAAAACCGTTTAACCCGGGGGACCTGCTGGCCCGTACCCGGGCGGTGCGGCGTCGTCGGGCGAACGAACTGCCGGGCGCACCGTCACAGGAGGGGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TCCA‑ACCTTACCCTCGGTAC‑G‑CGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  2:1640504/239‑1 (MQ=255)
                                                                   agTGGACCGTATCGTGGGCCTGGGGATTGGCGCTGACGACTACATTCCAAACCCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCg                                                                                                                                                                            <  1:184104/238‑1 (MQ=255)
                                                                       gACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACt                                                                                                                                                                                                                                                  <  2:1212969/167‑1 (MQ=255)
                                                                       gACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACt                                                                                                                                                                                                                                                  >  1:1212969/1‑167 (MQ=255)
                                                                        aCCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGGAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAAAGTTCCGCGAAGACGAGGCGAG‑GCCGCCCACCAGCGGTGAGTTTGCGGGAC‑Tg                                                                                                                                                                     >  1:1994029/1‑239 (MQ=255)
                                                                            tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATg                                                                                                                                                                                                                          <  2:1658851/184‑1 (MQ=255)
                                                                            tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATg                                                                                                                                                                                                                          >  1:1658851/1‑184 (MQ=255)
                                                                            tATCGTAGGCCTGGAGATTGGCGCTGACGACTACACTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑GAA‑TTTCTTTT‑GGGAAG‑TTTA‑AACTTAAACTCGGGAC‑G‑CGCGAAATGGTCCGCGCAGACGAGCCCGT‑GCCGCCCCCCAGCGGGGAGGTTGGGGGAC‑TGAAgg                                                                                                                                                                 >  2:686324/1‑239 (MQ=255)
                                                                              cggtgggCCGGGAGGTGGGCGCTGACGACCACCTCCAAAACCCGTTTACCCGGGGTAAATGGCGGCCCCGTACCGGGGCGGGGCGGGGGCGGCGGGCGAACGAACGGCAGGGCCCACGGCCGCAGGAAGAGGGGG‑TAA‑TTGCTTGC‑GGAAAG‑TACA‑AACTTACCCTCGGTAC‑G‑CGGGAAGTGTCCCGCGAAGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCa                                                                                                                                                               <  2:1586946/234‑1 (MQ=255)
                                                                                  gggCCGGGGGGTTGGCGCTGCCGCTAACATTCCAAACCCGTTTACCCGGCGTGAACTGCGGGCCCGTATCCGTGCGGTGGTGGGTCGTCAGGCGAACGAACTGCGAGGCGCCCCGTCACAGGAAGGGGCGG‑TAA‑TTGTTTTC‑GGTAAG‑TTCA‑AACTTACCCTGGGTAC‑G‑CGCGAATTGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTg                                                                                                                                                            <  2:2157101/237‑1 (MQ=255)
                                                                                    gCCTGGAGATTGGCGCTGACGGCGTCATTCCAAATCCGTTTACCCCGCGTGAACTGCTGGCCTGTATCCGTGGGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTG‑GGTAAT‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGGGAAGACGAGCCGAT‑GCTGCTCACCAGCGGTGAGTTTGCg                                                                                                                                                                            <  1:636831/221‑1 (MQ=255)
                                                                                     ccggtgaaTTGGCGCTAGCGACTACATTCAAAAACCTTTTAGCCCGGTTAAGCGGCTGGCCCGACTCCTGGCGGTGCTGCGTCTGCAGGCGAACGAACTGCAGGGCGACCCGCCCCAGGAGGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TACA‑AACTTACCCTCGGTAC‑G‑GGCGAAAGGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTc                                                                                                                                                         <  2:2195920/231‑1 (MQ=255)
                                                                                      cTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑ATGCTTTC‑GGTAAG‑TTCA‑AACCTAAACTCGGGAC‑G‑CGCGGAATGTTCCGCGGAGACGAGGCGAT‑GCCGCCCACCAGCCGGGGGGTTGCGGGACTTGAAGCCACGGGTc                                                                                                                                                         >  2:483264/1‑238 (MQ=255)
                                                                                      cTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑GAA‑ATGCCTTC‑GGGAAA‑TTCA‑AACTTAACCTCGGGAC‑G‑CGCGGAAAGGTCCCCCGAGACGACGCGAA‑GCCGCACCCCCGCGGCGAGGTTTCGGTAC‑TGGAGACagcggtac                                                                                                                                                        >  2:1358470/1‑231 (MQ=255)
                                                                                      cTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑CTA‑TTTCTTTC‑GGGAAA‑GTCA‑AACCTAACCTCGGTAC‑G‑CGCGAAATGTTCCGGGAAGACGGGCCGGT‑GCCGCCCACCAGCGGTGAGCTTGCGGGTC‑TTAAGGCACCGGTCa                                                                                                                                                        >  1:2171564/1‑238 (MQ=255)
                                                                                       tGGGGATTGGCGCTGCCGACTACATTCCAAAACCGTTTACCCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑ACCTTACCCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCg                                                                                                                                                                                                 <  1:1062786/197‑1 (MQ=255)
                                                                                       tGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGGAAG‑TTCA‑AACTTAACCTCCGTAC‑G‑CGCGAAATGTTCCGCGGAGACGAGCCGAT‑GCCGCCCACCAGCGGGGAGCTTTCGGGAA‑GGAAGGCACCGGGCAg                                                                                                                                                       >  1:1016187/1‑238 (MQ=255)
                                                                                         gagaTTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGGCGAT‑GCCCCCCCCCAGCGGGGAGGTTGCGGGTC‑TGAAGGGACTGGGCAGCCa                                                                                                                                                    >  1:1257183/1‑239 (MQ=255)
                                                                                           ggttGGCGCGTACGACTACATTCCAAAACCGTTTACCCCGCGTGAGCTGCTGGCCCGTCTCCGGGCGGTGCGGGGTCGTCAGGGGAACGACCTCCCGGGCGCACCGTCACAGGAAGGGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTCC‑G‑CGCGAAATGTCCCGGGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCAt                                                                                                                                                   <  2:1843924/236‑1 (MQ=255)
                                                                                                      acgacTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTT‑GGTAAG‑TTCA‑AACTTAACCCCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCCCCCCAGCGGGGGGTTTGGGGTAC‑TGAAGGGACCGGTGCGCCCTCCCC‑GTGAGcc                                                                                                                                       >  2:1192476/1‑238 (MQ=255)
                                                                                                      acgacTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑GTCA‑AACTTAACCTCGGGAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGcc                                                                                                                                       >  1:1911983/1‑238 (MQ=255)
                                                                                                      acgacTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑CAA‑TTGCTTTC‑CGTAAGTTTCA‑ACCTAAACCTCGG‑AC‑G‑CGCGAAAAGTTCCCCGAAGACGATCCCAA‑GCCCCTCACCAACGGTGAGGTTGCGGTAC‑TGAACGCTCCGGTCCGCCACACGC‑GCGCGcc                                                                                                                                       >  2:820470/1‑238 (MQ=255)
                                                                                                           tACATCCCAAACCCGTTTACCCGGCGTGACCTGCTGGCCCGTATCCGGGCGGGGTGGGGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTAC‑G‑CGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCa                                                                                                                                                               <  2:1778454/210‑1 (MQ=255)
                                                                                                                 ccAAACCCGTTTACCCCGCGGGAACTGCTGGCCCGTTTCCGTGGGGGGCGGGGTCGTCAGGCGAACGAACTGCCGGGCGCACCGTCACGGGAAGGGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGa                                                                                                                           <  2:2207651/239‑1 (MQ=255)
                                                                                                                   aaacCCTTTTACCCGGCGTGACCTGCTGGCCCGTCTCCGGGGGGGGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCAGAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TACA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATa                                                                                                                         <  2:1016187/239‑1 (MQ=255)
                                                                                                                   aaaaCCGTTAACCCCGCGGGACCGGCGGGCCCGTGCCCGGGCGGTGCGGCGTGGTCAGGCGAACGACCGGCCAGGCGCCCCGTCACAGGAAGGGGGGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTAC‑G‑CGCGAATTGTCCCGGGAGGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATa                                                                                                                         <  2:80884/239‑1 (MQ=255)
                                                                                                                     acccttttACCCCGCGTGACCTGCGGGCCCGTTCCCGGGCGGTGCGGCGTCGTCAGGAGAACGAACGGCCAGGCGCACCGTCACAGGAAGGGGCGG‑TAA‑GTGTTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTCC‑G‑CGCGAAAGGTCCCGGGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAg                                                                                                                       <  2:362180/234‑1 (MQ=255)
                                                                                                                     aaCCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGGCAGCCATCCGC‑GGGAGCCGCTCTCCCGCGATaa                                                                                                                        >  1:80884/1‑238 (MQ=255)
                                                                                                                     aaCCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGGTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCCGcc                                                                                                                                                     >  1:1778454/1‑210 (MQ=255)
                                                                                                                          tttAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGGAC‑G‑CGCGAAATGTTTCGCGAAGACGAGCCGAT‑GCCGCCCACCAGCGGTGAGTTTGCGGGAC‑TGAAGGCACTGGTGAGCCCTCCGC‑GGGAGGCGCCCTCCCCCGATAAGCt                                                                                                                     >  2:2142893/1‑236 (MQ=255)
                                                                                                                            tAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCCCACCAGCGGGGAGTTTGCGGTAC‑TGAAGGCACCGGTCAGCCCTCCGC‑GTGAGCCGCTCCCCCGCGATAAACtgatga                                                                                                                >  2:1747696/1‑239 (MQ=255)
                                                                                                                                  gcgTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGGAAA‑TTCA‑AACTTAAACTCCGTCA‑G‑CGCGGAATGTTCCGCGGAACCGCGCCCATCGCCATCCACC‑CCGGGGGGGGTGCGGGAC‑TGCAAGGACTCGTGTGCCCTTCGC‑CGGGGGCGCGCTCCCGCGCTAAACTGATGAACCTTg                                                                                                          >  2:1613254/1‑239 (MQ=255)
                                                                                                                                    gTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGGTTGCGGGAA‑TGAAGGCACTGGTCAGCCATCCGC‑GTGGGCCGCTCTCCcgcg                                                                                                                            >  1:587563/1‑219 (MQ=255)
                                                                                                                                      gAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCg                                                                                                      <  1:1228008/239‑1 (MQ=255)
                                                                                                                                      gAACGGCGGCCCCGTACCGGGGCGGTGCGGCGTCGTCAGGCGAAAGAGCTGCCGGGCGCACCGTCAGAGGAAGGGGGGG‑TAA‑TTGCTTTC‑GGTAAG‑TTAA‑AACTTAACCTGGGTAC‑G‑GGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCg                                                                                                      <  2:2251150/239‑1 (MQ=255)
                                                                                                                                       acctcgtcgCCGGTATCCGGGCGGTGCTGGGTCGCCAGGGGAACGAACAGCCGGGCGCACGGTCACAGGAAGGGGGGG‑TAA‑TTGTTTTG‑GGTAAG‑TCCA‑AACTTACCCTCGGCAC‑G‑GGCAAAATGTCCCGCGAAAACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCg                                                                                                      <  2:1090312/230‑1 (MQ=255)
                                                                                                                                          tgctgGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTACCCTCGGTCC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGt                                                                                                                                                                          <  1:105671/169‑1 (MQ=255)
                                                                                                                                            cgggCCCGTGCCCGGGGGGGGGTGGGTCGTCGGGCGAACGAACTGCCAGGCGCACCGTCACGGGAAGGGGGGG‑TAA‑TTGCTTTC‑GGTAAG‑TAAA‑AACTTACCCTCGGTAC‑G‑CGAGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTc                                                                                                 <  2:2168683/236‑1 (MQ=255)
                                                                                                                                                  tgTACCCGGGCGGTGTGGGGTCGTCAGGCGAACGAACTGCCGGGCGACCGGTCACGGGAAGGGGGTG‑AAA‑TTGCTTTC‑GGAAAT‑TTCA‑ACTTTACCCTGGGTCC‑G‑CGCAAAATGTCCCGGGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGTCCGTGGTCGTGAata                                                                                          <  2:33055/238‑1 (MQ=255)
                                                                                                                                                     aTCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCAAAGGAAGAGGCGG‑TAA‑TTGCTTGC‑GTTAAG‑TTCA‑AACTTACCCTCGGTCC‑G‑CGCGAAATGTTCCGCGAAGAGTAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATAtt                                                                                        <  1:1864657/238‑1 (MQ=255)
                                                                                                                                                      cccGTGCGGGGGTGAGTCGTCAGGCGAACGAACTGCCAGGCGCCCCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑t                                                                                                                                                                      <  1:834567/159‑1 (MQ=255)
                                                                                                                                                          tGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAA‑GTTA‑AACTTAACCTCGGTAC‑G‑AGCGAAATGTTCCGCGAAGACGAGCCGAG‑GCCGCCCACCAGCGGGGGGTTTGCGGTTC‑TGAAAGCACTGGTCAGCCATCCTC‑GCGAGCCGCCCCCCCGCGATAAGCTGATGAACCTTGCCCGGGGTCCTGACAATTccccc                                                                                   >  2:1457270/1‑235 (MQ=255)
                                                                                                                                                          tGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGGAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CCCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCCCCAGCGGGGAGTTTTCGGTAC‑TGAAGGCACTGGTCACCCACCCCC‑GGGAGCCGCTCCCCCCCGAAAAGCTGATGAACCTTGCCCGGGGGGGGGCATATTCCGCac                                                                                  >  2:1216670/1‑238 (MQ=255)
                                                                                                                                                           gCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑TGTAAG‑TTCA‑AACTTTACCTCGGTAC‑G‑CGCGAAATTTTCCCCGGAGACGAGCCGAT‑GCCGCCCACCCGCGGGGAGGTTTCGGGAC‑TGAAGGGACCGGTCAGCCATCCCCGGTGA‑CCGGCCTCCCCCCCTAAACTCATGAACCTTTCCCCTGGGCGTGAATAATCCCCAAt                                                                                 >  2:1695912/1‑239 (MQ=255)
                                                                                                                                                                 ctgcGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑GTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGGGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCCCCCCCGCGATAAACTGATGAACCTTGCCCGGGGGCGTGAATATTCCGCAATGGAAc                                                                            >  1:2266192/1‑238 (MQ=255)
                                                                                                                                                                    cgtcgtCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑ATGCTTTT‑GGTAAG‑TTCC‑AACTTAACCTCGGTAC‑GCCGGGAAAT‑TTTCGCGGAGACGAGCCGAT‑GGCGCCCACCACCCGTGGGGTTGCGGGAC‑TGAAGGCCCTCGTCCGCCCCCCGC‑CGGAGGCGCTCCCCCGCGCTAAGCAGATGAAACTTGTCCGTGGTCGTGAATATTCCGCAATGGAAAGCt                                                                         >  2:1140012/1‑238 (MQ=255)
                                                                                                                                                                         tcAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTTA‑AACCTAACCTCGGGAC‑G‑CGCGGAATGGTTCGCGGAGACGAGCCGAT‑GCCGCCCACCAGCGGGGAGGTTTCGGGAC‑TGAAAGGCCTGGGCCGCCCTCCCC‑GCGAGCCGCCCTCCCGCGATAAGCTGATGAACCTTGCCCGGGGGCGTGAATATTCCGCAAAGGAACGCCCCCTc                                                                    >  2:1902847/1‑238 (MQ=255)
                                                                                                                                                                           aGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGGGAGTTTGCGGTAC‑TGaa                                                                                                                                                                   >  1:2144971/1‑142 (MQ=255)
                                                                                                                                                                                                    gTCACGGGGAGGGGGGG‑TAT‑TTTCTTTG‑GGTAAT‑TCAA‑AACTCACCCTGGGTAC‑G‑CGGGAAATGTCCGGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCa                                        <  2:1257183/239‑1 (MQ=255)
                                                                                                                                                                                                    gTCACAGGAGGGGGCGT‑TAA‑TGGTTTTC‑GGTAAG‑TCAA‑AACTCACCCTGGGTAC‑G‑GGCGAATTGTCCGGGGAGGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCa                                        <  2:1644720/239‑1 (MQ=255)
                                                                                                                                                                                                     tCACAGGAAGAGGGGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑ACCTTAACCTCGGTCC‑G‑CGCGAAATTTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATc                                                                    <  2:1975153/210‑1 (MQ=255)
                                                                                                                                                                                                     tCACAGGAAGAGGCGG‑TAA‑TTGTTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTCC‑G‑GGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGt                                                                                                                                                                                     <  1:819869/99‑1 (MQ=255)
                                                                                                                                                                                                           gAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑ATCA‑AACTTAACCCCGGGAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCCCCAGCGGGGAGGTTGCGGGAC‑TGAAAGCACTGGTCAGCCATCCGC‑GGGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGGCGTGAATATTCCGCAATTGAACGCTCCCTCCGCGTGCAGATTTCGCGTCTGCGCCGCCcggtggc                                 >  2:1505938/1‑238 (MQ=255)
                                                                                                                                                                                                            aaGAGGCGG‑TAT‑TTGCTTTC‑GGTAAG‑TTAA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGt                                                                                               <  1:43769/176‑1 (MQ=255)
                                                                                                                                                                                                               gggCGG‑TAA‑GTGCTTTA‑GGTAAG‑TTCA‑ACCTTAACCTCGGTAG‑G‑CGCGAAATGTTCGGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGgaaga                              <  2:588127/237‑1 (MQ=255)
                                                                                                                                                                                                               agGCGG‑TAA‑TTTCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGGAATGTTCCGCGGAGACGAGCCGAT‑GCCGCTCACCCGCGGGGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGCTAAACTGATGAAACTTGCCCGGGGTCGTGAAAATTCCGCCACGGGAAGCTCCCTCGACCTGCAGATTTCGCGCCTGCGCCGCAAGGGGgaagaa                             >  2:830770/1‑239 (MQ=255)
                                                                                                                                                                                                               agGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTGC‑G‑CGCGAATTGTTCCGCGAAGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGgaaga                              <  1:411844/238‑1 (MQ=255)
                                                                                                                                                                                                               agGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCCCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GGGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGGGGTCGGGAATATTCCGCAATGGAACGCTCCATCGACGGGCAGATTTCGCGTCTGCGCCGCATGGTGgaagaa                             >  1:1016663/1‑239 (MQ=255)
                                                                                                                                                                                                               agGCGG‑TAA‑TTGCTTTC‑GGTAAG‑GTCA‑AACTTAACCCCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGGGGGTTTGCgg                                                                                                                                                                           >  2:819869/1‑99 (MQ=255)
                                                                                                                                                                                                                 gCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGGAATGTTCCGCGAAGACGAGCCGAT‑GCCGCCCCCCAGCGGGGAGATTGCGGGAC‑TGAAGGCACTGGTCAGCCATCCGC‑GGGAGCCGGTCTCCCGCGATAAGCTGATGAACCTTCGCCGGGGTCGGGAATATTCCCCCATGGAACGCCCCATCGACCGGCCGATTTCTCGTCCGCGCCGCAtggggggcaaaga                           >  2:1934327/1‑232 (MQ=255)
                                                                                                                                                                                                                          ttGCTTTC‑GGTAGG‑TCCA‑ACCTTACCCTCGGTGC‑G‑CGCGAAATGTCCGGCGAAGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAgcg                    <  2:2136237/239‑1 (MQ=255)
                                                                                                                                                                                                                              tttG‑GGTAAG‑TGCA‑ACTGAAACCTGGGTAG‑G‑CGGGAAAGGTTCCGCGAAGGCGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCAt                 <  2:31160/238‑1 (MQ=255)
                                                                                                                                                                                                                                    gTAAG‑TTCA‑ACCTTACCCTCGGCAC‑G‑GGGGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGT‑GTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt           <  1:1747696/238‑1 (MQ=255)
                                                                                                                                                                                                                                    gTAAG‑TCAA‑ACCTTACCCTCGGTAC‑G‑CGCGAAATGTCCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  2:357586/78‑1 (MQ=255)
                                                                                                                                                                                                                                     tAAG‑TCAA‑AGCTTAACCTGGGTCC‑G‑CGGGAAAGGTTCCGAGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt           <  2:1996100/238‑1 (MQ=255)
                                                                                                                                                                                                                                     tAAG‑TAAA‑AACTTACCCTGGGTAC‑G‑CGAGAATTGTCCGGCAAGGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt           <  2:2233735/238‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaG‑TTCA‑AACTGACCCTCGGTAC‑G‑CGCTAGATGTGCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt           <  1:1325326/237‑1 (MQ=255)
                                                                                                                                                                                                                                            cA‑AACTTACCCTCGGTCC‑G‑CGCGAAATGTTCGGCGAGGAGGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTc    <  1:820470/239‑1 (MQ=255)
                                                                                                                                                                                                                                            cA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTc    >  1:342850/1‑239 (MQ=255)
                                                                                                                                                                                                                                               aaCTTACCCTCGGTAC‑G‑CGCGAATTGTGCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAg  <  1:1391927/239‑1 (MQ=255)
                                                                                                                                                                                                                                               aaCTTACCCTCGAGGC‑G‑CGGGAAGGTTTCCGGGAAGAGGAGCCGAT‑GCCGCTCCCCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAg  <  2:1994029/239‑1 (MQ=255)
                                                                                                                                                                                                                                                aCTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              <  2:1700872/68‑1 (MQ=255)
                                                                                                                                                                                                                                                aCTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTg                                                                                                                                                                              >  1:1700872/1‑68 (MQ=255)
                                                                                                                                                                                                                                                aCTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGaa                                                                                                                                                                   >  1:357586/1‑78 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
TTTGCCGACGTCTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTGAACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGG‑TAA‑TTGCTTTC‑GGTAAG‑TTCA‑AACTTAACCTCGGTAC‑G‑CGCGAAATGTTCCGCGAAGACGAGCCGAT‑GCCGCTCACCAGCGGTGAGTTTGCGGTAC‑TGAAGGCACTGGTCAGCCATCCGC‑GTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAG  >  NC_007779/4104028‑4104500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑