BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	4,248,114	A→C	6.8%	S184R (AGT→AGG)	malF ←	maltose ABC transporter permease MalF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,248,114	0	A	C	6.8%	381.0 / 2.8	150	S184R (AGT→AGG)	malF	maltose ABC transporter permease MalF
Reads supporting (aligned to +/- strand): ref base A (77/58); new base C (10/3); total (88/62)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCG  >  NC_007779/4247888‑4248352

cGGTGGTCCCGGGTGAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGa                                                                                                                                                                                                                                         <  2:1343353/239‑1 (MQ=255)

cGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGCGCGGCGGCGTGCCAGAAAACTGGCGCAGGGAGATCAACATGACTTTTTTGCCATCCGGCAGACTGGC‑GGGAA‑GGCCACCCAGCACCTg                                                                                                                                                                                                                                          >  2:155742/1‑238 (MQ=255)

   tggtCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAATTGTC‑CTCCGCGCCTGACGa                                                                                                                                                                                                                                      >  1:205407/1‑239 (MQ=255)

                    gggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCAACCAGCGCCCCGCGGCTCTGGGGAATCAccc                                                                                                                                                                                                                      >  2:620457/1‑236 (MQ=255)

                     ggCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAAAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCAcgcg                                                                                                                                                                                                                     <  1:1671694/238‑1 (MQ=255)

                      gCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCCTCATCACTTTGTTGCCCTCCGGCAGAATGGCGGGGAA‑GTTCACCCTGCGCCTGCCGATTCTGGGaaaaccagcgc                                                                                                                                                                                                                    >  2:1139213/1‑231 (MQ=255)

                      gCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTTACCCACCCCCCGGCGAATTTTGGTaaaaacacccc                                                                                                                                                                                                                   >  2:2002598/1‑230 (MQ=255)

                               ttCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGGA‑AGGCACTCCGCGCCTTAAGAATCTTGGTGATCACCCGGAGATgcgcgc                                                                                                                                                                                                          >  1:1416496/1‑239 (MQ=255)

                                  atcaCCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGtt                                                                                                                                                                                                       <  2:1434707/239‑1 (MQ=255)

                                      ccccAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGCAA‑GGGTACCCCTGGCCCTGCGCTTCTGGGGGATCACGCGCAGATTCGCGGGTTCGc                                                                                                                                                                                                    >  1:1492506/1‑238 (MQ=255)

                                       cccAGTTGCCGTCGGCGGTAATGGACTGGTAAATGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCt                                                                                                                                                                                                  <  1:502764/239‑1 (MQ=255)

                                           gTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGGAAA‑GGTGAC‑CAGCGCCCGTCGAGTCTTGGTAATAACGCCCCGGCTTGCGCGTGCGCCCTccg                                                                                                                                                                                              >  2:1536474/1‑237 (MQ=255)

                                                       ggTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTc                                                                                                                                                                                   <  1:81733/238‑1 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCATGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAC‑TGTCACCCCGCCCCTGACGGTTCTGGGTAATCACCCCCAGATTCGCGCGTTCGCCTTCGGGCTGGGGGGGC‑GTTTCTTTCAggt                                                                                                                                                                     >  2:629546/1‑237 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCCCTCCGCGCCTGTCGCTTCTTGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GGTTCTTTCAGtt                                                                                                                                                                     >  1:988971/1‑239 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGtt                                                                                                                                                                     >  1:1534025/1‑239 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGACTCTGGGGAATCACGCGCAGAATCGCCCCTTCCCCCTCCGGGCGGGGGGGG‑Gtttttttccggt                                                                                                                                                                     >  2:235461/1‑235 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCTGACGGTTTTGGGGAATCAACCGCACATTCGCGCGGTTCCCCTCGGGGTGGGGGGGG‑GGTTTTTTCAGtt                                                                                                                                                                     >  2:1908741/1‑239 (MQ=255)

                                                                   gTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCTGACGATTCTTGGTAATCACGCGCAGATTCGCGCGTTTGCCCTCGGGCGGGGGGGGC‑GTTTCTTTCAGtt                                                                                                                                                                     >  2:2171112/1‑239 (MQ=255)

                                                                    tAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCGCCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCCCTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGtt                                                                                                                                                                                                       <  1:859195/205‑1 (MQ=255)

                                                                             aTTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTTCCCCAGCCCCCGACGGTTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGGGGGG‑GGTTCTTTCTGGTGCAGGTTTTg                                                                                                                                                           >  2:372625/1‑239 (MQ=255)

                                                                             aTTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTg                                                                                                                                                           >  1:357838/1‑239 (MQ=255)

                                                                             aTTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACCCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GGTTCTTTCCGTTGCAGTTTTTg                                                                                                                                                           >  1:1942073/1‑239 (MQ=255)

                                                                             aTTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGGCCCTCAGCGCCTGTCGATTCTGGGGAAACACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GTTTTTTTCAGGTGCAGgtttt                                                                                                                                                            >  1:1071078/1‑238 (MQ=255)

                                                                             aTTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGGCACCCCGCGCCTGACGGTTCTGGGGAATCACGCGGAGATTCGCGCGGTTGCCCTTGGGGCGGGTGGGT‑GTTTCTTTCAGATGCCGgtttt                                                                                                                                                            >  2:714948/1‑238 (MQ=255)

                                                                              tttGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGc                                                                                                                                                                   <  1:630350/230‑1 (MQ=255)

                                                                              tttGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGGCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GGTTCTTTCCGTTGCAGttttt                                                                                                                                                            >  1:123487/1‑237 (MQ=255)

                                                                                   ttATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTCA‑TGTCACTCCGCGCCCGACGATTCTGGGGAATCACGCGCAGATTCGCGCGGTCGCCCTCCGGCTGGGCGGGC‑GTTTCTTTTAGGTTCAGTTTTTG‑CTcggc                                                                                                                                                    >  1:718275/1‑237 (MQ=255)

                                                                                      ttCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCg‑ccgcc                                                                                                                                                <  1:2002598/239‑1 (MQ=255)

                                                                                        cGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCaa                                                                                                                                              <  1:235461/239‑1 (MQ=255)

                                                                                           aCGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAAtt                                                                                                                                           >  1:419687/1‑239 (MQ=255)

                                                                                                  ttCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTTCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GTTTTTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGc                                                                                                                                    <  1:1705900/239‑1 (MQ=255)

                                                                                                        ccgcTCTGATTATTCGTCAACGTGCCGTCCCCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGga                                                                                                                              <  2:1058404/239‑1 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCTCCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGga                                                                                                                              <  1:139598/238‑1 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCACCGTCGTGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGGTTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GGTTCTTTTCGTTGCAGTTTTTG‑CTCG‑CCGCCCAACTTAAAACGGTCgggg                                                                                                                             >  2:607910/1‑237 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCCCTCAACGCCTGACGATTCTGGGGAATCAAGCCCCGCATGCCGCGGTCGCCCTCGGGGTGGGCGGGCTGTTTC‑TTCAGTTTCAGTTTTTGTCCCG‑CCCCCAATTTGaaaacggccgag                                                                                                                              >  2:1705900/1‑231 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCAccc                                                                                                                                                                                                                                                  >  1:753196/1‑123 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTc                                                                                                                                                                                                                                                  <  2:753196/125‑1 (MQ=255)

                                                                                                         cgcTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGGCCCTCCGCGCCTGACCATTCTGGGGAATCACGCGCCGAATTGCGCGGTCCCCTTCCGGCCGGGGCGGC‑GTTTCTTTCAGTTGCAGGTTTTT‑TCCG‑CCGCCCAATTTTAAAACggccgga                                                                                                                             >  2:1231624/1‑233 (MQ=255)

                                                                                                           ctctGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGt                                                                                                                                  <  1:1897320/232‑1 (MQ=255)

                                                                                                           ctctGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCTGACGGTTCTGGGGAATCACGCGCAGATTCGCGCGGTCGCCTTCGGGCTGGGGGGGT‑GTTTTTTTTAGGTGCGGGTTTTG‑GTTC‑CCGCCCAATTTAAAAGCGGCGGgga‑g                                                                                                                          >  1:1339903/1‑239 (MQ=255)

                                                                                                            tctGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCCCTCAGCGCCCGACGGTTCTGGGGAATCCCGCGCAGATTCGCGCGTTTCCCTTCGGGCTGGGGGGGC‑GTTTCTTTTCGGTGCCGTTTTTG‑CTCG‑CCGCCAAATTTATAAGCGTCGGAGA‑gg                                                                                                                         >  2:1067556/1‑239 (MQ=255)

                                                                                                            tctGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGGCACCCAGCGCCCGACGATTCTGGGGAATCACGCGCCGATTCGCGCGGTCCCCCTCCGGCTGGGCGGGC‑GGTTCCTTTCCTTTCAGGTTTTGCCTGC‑CCCCCAAATTAAAAACCGTGGgaggg                                                                                                                           >  2:2103405/1‑237 (MQ=255)

                                                                                                                attattCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGga                                                                                                                              <  1:1890145/231‑1 (MQ=255)

                                                                                                                  tattCGTCAACGTGACGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTcg                                                                                                                                                      <  1:2185181/206‑1 (MQ=255)

                                                                                                                     tCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCCGGGCGGTC‑GTTTCTTTCCGTTGCAGTTTTTT‑CTCG‑CCGCCCAATTTAAAAACGTCGGGGG‑GGGAAATTTTg                                                                                                                >  2:2193839/1‑239 (MQ=255)

                                                                                                                     tCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACCCAGCGCCTTACGATTCTGGGGAATCACGCGCAGATTCGCGCCTTCGCCCTCGGGCCGGGGGGGC‑GGTTCTTTCAGTTGCGGTTTTTG‑TCCG‑CCGCCCAATTTAAAAGAGGCGGAGA‑GGTaaacgtc                                                                                                                 >  2:1897320/1‑233 (MQ=255)

                                                                                                                        tCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GTTTCTTTCCGGTGCAGTTTTTG‑CTCG‑CCGCCCAATTTAAAAGGGTCGGGGAGGGTAAtttttccg                                                                                                              >  2:177982/1‑236 (MQ=255)

                                                                                                                        tCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCCGACGATTCTGGGGAATCACGCGCCGATTCGCCGGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑TTCG‑CCGCCCAATTTAAAAGCGTCGGGGA‑GGTAATTTTTTCCg                                                                                                             >  2:1231789/1‑239 (MQ=255)

                                                                                                                        tCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCACCGCCTCACGCATCTGGGTAATCACGCCCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GCTTCTTTCACTTCCCGGTTTTT‑CTCG‑CCCCCCACTTTAAAAGCGTCGGTGC‑GGTAACTTTTTCCg                                                                                                             >  2:2216768/1‑239 (MQ=255)

                                                                                                                        tCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGGCCCTCAGCGCCTGACGATTCCGGGGAATCACGCGCGGGTTCGCGCGGTCGCCCTCCGGCTGGGCGGGC‑GTTTTTTTCCGTTTCCGTTTTTG‑CGCG‑CCGCCCAATTTAAAAACGTCGGAGA‑GGGAAtttttttcg                                                                                                             >  2:376328/1‑235 (MQ=255)

                                                                                                                         cAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAGATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCgg                                                                                                            <  1:1621644/239‑1 (MQ=255)

                                                                                                                          aaCGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑AGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCCGATTTGCGCGTTCGCCTTCGGGCGGGGCGGTC‑GTTTCTTTTCGGTGCAGGTTTTG‑CTCG‑GCCCCCAATTTAAAAACGTCGGGGG‑GGTGATTTTTGCCGGt                                                                                                           >  2:4465/1‑239 (MQ=255)

                                                                                                                          aaCGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCCGCCGATTTTGGGGAATAACGCGCAGGATCGCGCGTTCGCCCTCCGGCTGGGGGGTC‑GTTTCTTTCAGTTTCAGGTTTTG‑CTCG‑GCCCCCAATTTAAAAGCGTCGGGGG‑GGGTAttttttttc                                                                                                             >  2:1890145/1‑233 (MQ=255)

                                                                                                                            cGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAgcg                                                                                                                                                                                                                                              <  2:444120/110‑1 (MQ=255)

                                                                                                                            cGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAgcg                                                                                                                                                                                                                                              >  1:444120/1‑110 (MQ=255)

                                                                                                                            cGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCCGATTCGCGCGGTCGCCTTCGGGCCGGGCGGGC‑GTTTCTTTCAGTTGCAGTTTTTT‑CTCG‑CCCCCCAAtt                                                                                                                                           >  2:2185181/1‑206 (MQ=255)

                                                                                                                                   tCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGGATCTGGGGAATCCCGCGCAGATTCGGGCGGTCGCCTTCCGGCCGGGCGGTG‑GTTTCTTTCAAGTGCAGGTTTTG‑CTCG‑CCGCCCCACTTAAAAGCGGAGGAGA‑GGGCATTTTTTCCGGTTTTCCCGt                                                                                                   >  2:1529461/1‑238 (MQ=255)

                                                                                                                                          cGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAg                                                                                            <  1:758048/238‑1 (MQ=255)

                                                                                                                                              tgtgtAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCTCGCGTTCTCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGcc                                                                                        <  1:404459/238‑1 (MQ=255)

                                                                                                                                                       cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAAACACCCGCCGATTTGCGCGTTCGCCTTCGGGGCGGGCGGGC‑GTTTCTTTTAGTTGCAGTTTTTG‑CTCG‑CCGCCCAATTTAAAAGCGGTGGGGG‑GGGAAATTTTTCCGGTTTCGCCGTCGCTGAGCGCCCGTTTcccc                                                                               >  2:1892931/1‑236 (MQ=255)

                                                                                                                                                       cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑AGGCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCCGTTTCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGGGA‑GGTAATTTTTGCCGGGTTCGCCCTCCCTGAGCCCCCGGTGCCAc                                                                               >  2:562815/1‑238 (MQ=255)

                                                                                                                                                       cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCTGACGGTTCTGGGTAATTACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GTTTCTTTCAGTTGCAGGTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGGGG‑GGGAATTTTTGCCGGTTTTGCCGTCGCTGAGCGCCAGTTggcca                                                                               >  2:867160/1‑234 (MQ=255)

                                                                                                                                                          cTGCGTGCCAGAAAACTGGCGCAGTGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGATGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑gg                                                                                                                         <  1:1378651/193‑1 (MQ=255)

                                                                                                                                                            gCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCg‑ccgcc                                                                                                                                                >  2:283732/1‑169 (MQ=255)

                                                                                                                                                            gCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCg‑ccgcc                                                                                                                                                <  1:283732/169‑1 (MQ=255)

                                                                                                                                                               tGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCCAATTTAAAAGCGTCGGGGG‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcc                                                                       >  1:19610/1‑238 (MQ=255)

                                                                                                                                                                  cAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                   <  1:714948/239‑1 (MQ=255)

                                                                                                                                                                       aaCTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTa                                                              <  1:376328/239‑1 (MQ=255)

                                                                                                                                                                            gcgcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑AGTCCCTCAGCGCCTGCCGGTTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GGTTCTTTCAGTTGCAGATTTTG‑CTCG‑CCGCCCAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTTGCCGTCCCCGAACGCCAGTTGCCCCCCATCTCCCCCCGTGTTAAGa                                                          >  2:2145750/1‑238 (MQ=255)

                                                                                                                                                                             cgcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAc                                                         <  1:177982/238‑1 (MQ=255)

                                                                                                                                                                              gcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCa                                                       <  1:4465/239‑1 (MQ=255)

                                                                                                                                                                              gcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCa                                                       <  2:108438/239‑1 (MQ=255)

                                                                                                                                                                               cAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCa                                                       <  2:296856/238‑1 (MQ=255)

                                                                                                                                                                                     gccactcctcaCTTGGTTTCCATCCGGACGAATGGG‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTta                                                <  2:1416496/230‑1 (MQ=255)

                                                                                                                                                                                     gCTCATCATCGCTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCCCTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTta                                                <  1:579519/238‑1 (MQ=255)

                                                                                                                                                                                              caCTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTg                                       >  1:1902406/1‑238 (MQ=255)

                                                                                                                                                                                              caCTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGTC‑GTTTCTTTTAGTTGCAGGTTTTG‑CTCC‑CCGCCCAATTTAAAAACGTCGGAGA‑GGGAATTTTTTTCGGTTTCCCCCTCGCTTGGCGCCAGTTGCCCCTCACTCTCCGCCGGGGAAAGACCA‑AAGTTTAAGGctttt                                       >  2:2190749/1‑237 (MQ=255)

                                                                                                                                                                                              caCTTTGTTGCCATCCGGCAGAATGGC‑GGGAA‑TGTCCCTCAGCGCCTTACGATTCTGGGGAATCACGCGCAAATTCGCGCGGTCGCCTTCCGGGTGGGCGGGC‑GGTTCTTTTAGTTGCAGGTTTTG‑CTCGCCCGCCAAATTAAAAAG‑GGCGGAGA‑GGGAATTTTTTTCGGTTTCGCCGTCGCTTAGGCCCCGTTTCCACCCCACGCCCGCCGGGTAAATACCA‑AAGGTTTAGGgtttt                                       >  2:1720650/1‑237 (MQ=255)

                                                                                                                                                                                                cTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTg                                       >  1:189600/1‑236 (MQ=255)

                                                                                                                                                                                                 tttGTTGCCGTCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACTCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCGTTCAGTTGCGGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTg                                   <  2:511747/239‑1 (MQ=255)

                                                                                                                                                                                                 tttGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCCCTCAGCGCCGGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTg                                   <  1:1046369/239‑1 (MQ=255)

                                                                                                                                                                                                 tttGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTg                                   <  1:607910/239‑1 (MQ=255)

                                                                                                                                                                                                   tgttgCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCg                                                                                                    >  1:1003871/1‑173 (MQ=255)

                                                                                                                                                                                                   tgttgCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCg                                                                                                    <  2:1003871/173‑1 (MQ=255)

                                                                                                                                                                                                   tgttgCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTTCCGGTTTTG‑CTCG‑CCGCCAAATTTTAAAGCGTCGGGGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTTAGCGCCCGTTGCCACTCATCCCCCGCCGGGTAAAGACCC‑AAGGTATAGGGTTTGCCTg                                   >  2:520436/1‑237 (MQ=255)

                                                                                                                                                                                                   tgttgCCATCCGGCAGAATGGC‑GGTAA‑TGTCACCCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GTTTCTTTCAGTTGCAGGTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCt                                 >  1:1756615/1‑239 (MQ=255)

                                                                                                                                                                                                   tgttgCCATCCGGCAGAATGGC‑GGGAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GTTTCTTTCAGTTGCAGGTTTTG‑CTCG‑CCGCCCAATTTAAAAGCGTCGGAGA‑GGTAATTTTTTCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCC‑AAGTTTTAGGTTTTGGCTg                                   >  2:742708/1‑237 (MQ=255)

                                                                                                                                                                                                     ttgCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGGTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGGGA‑GGGAATTTTTTCCGGTTTCGCCCTCGCTGAGCGCCAGTTGCCCCTCATCGCCCGCCGGGTAAAGACCA‑AAGGTATAGGTTTTGCcttctt                                >  2:77599/1‑238 (MQ=255)

                                                                                                                                                                                                        ccATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGGC‑GTTTCTTTCAGTTGCCGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGGGATTTTTGCCGGTTTTGCCCTCGCTGAGCGCCCGTTGCCACTCATCGCCCGCCGGGTAAAGACCC‑AAGTTATAGGGTTTGGCTGCtttgca                            >  2:1046369/1‑235 (MQ=255)

                                                                                                                                                                                                         cATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCa                            <  1:155742/238‑1 (MQ=255)

                                                                                                                                                                                                           tCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGGAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCCGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGGGA‑GGGAATTTTTGCCGGTTTTCCCGCCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGGGAAGACCACAGGTTATA‑GTTTTGCCTGCTTGCCCggc                         >  2:332669/1‑238 (MQ=255)

                                                                                                                                                                                                            ccGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAg                        <  1:1892931/239‑1 (MQ=255)

                                                                                                                                                                                                             cGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGc                       <  1:1820083/239‑1 (MQ=255)

                                                                                                                                                                                                                cAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGa                     <  2:205407/238‑1 (MQ=255)

                                                                                                                                                                                                                cAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGa                     <  2:1783008/238‑1 (MQ=255)

                                                                                                                                                                                                                        c‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa             <  1:2103405/238‑1 (MQ=255)

                                                                                                                                                                                                                          ggTAA‑TGTCTCTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca           <  1:495622/239‑1 (MQ=255)

                                                                                                                                                                                                                              a‑TGTTACTCAGCGCCTGACGATTTTGGGTAATCACGCGCAGATTCGCGCGTTCGCCCTCGGGCTGGGCGGGC‑GTTTCTTTCCGTTGCAGTTTTTG‑CTCG‑CCGCCCAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCCCCCTCGCTGAGCGCCAGTTGCCCCCCATCGCCCGCCCGGGGAAGACCC‑AAAGTATAGGTTTTGCCTGCTTGCCAGCGGGGATCTACCAacacct        >  2:209465/1‑236 (MQ=255)

                                                                                                                                                                                                                                    aCTCAGCGCCTGGCGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgcg  <  1:629546/239‑1 (MQ=255)

CGGTGGTCACGGTGTAACCGGGGCTTAGCTTTTCATCACCCCAGTTGCCGTCGGCGGTAATGGACTGGTAAAAGCCAATTTGGTTATTCGGACGATATTTCACGCCGCTCTGATTATTCGTCAACGTGCCGTCACCGTCGAGTGTGTAGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGC‑GGTAA‑TGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTC‑GTTTCTTTCAGTTGCAGTTTTTG‑CTCG‑CCGCCAAATTTAAAAGCGTCGGAGA‑GGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCA‑AAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCG  >  NC_007779/4247888‑4248352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`