BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,788	G→T	10.7%	T34N (ACT→AAT)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,788	0	G	T	10.7%	276.2 / 6.0	137	T34N (ACT→AAT)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base G (76/38); new base T (2/14); total (84/53)
Fisher's exact test for biased strand distribution p-value = 4.67e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954561‑955017

       ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  1:363093/239‑1 (MQ=255)

       ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  2:1788048/239‑1 (MQ=255)

       ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCACCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  2:357738/239‑1 (MQ=255)

        cAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGt                                                                                                                                                                                                                     <  2:2141092/239‑1 (MQ=255)

          ggTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  2:1016972/236‑1 (MQ=255)

          ggTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGCTTCg                                                                                                                                                                                                                      >  1:1016972/1‑236 (MQ=255)

            tGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGtt                                                                                                                                                                                                                    <  1:610511/236‑1 (MQ=255)

               tGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTta                                                                                                                                                                                                              >  1:179917/1‑239 (MQ=255)

                gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTtat                                                                                                                                                                                                             <  2:2183766/239‑1 (MQ=255)

                gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTtat                                                                                                                                                                                                             >  1:1314859/1‑239 (MQ=255)

                   cccACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCCGAT‑AGAAAGGCTTAAGCGGGTGGTTCGTTGGTTTATAg                                                                                                                                                                                                           >  1:1629599/1‑238 (MQ=255)

                        tCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTTTTGAAAcc                                                                                                                                                                                                      >  1:2212979/1‑238 (MQ=255)

                                      acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCctc                                                                                                                                                                                                >  1:1414367/1‑230 (MQ=255)

                                      acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGAGGTTTTGGTGCTTTATAGAAACCCGCCCCTTCGGcc                                                                                                                                                                                         >  1:818228/1‑237 (MQ=255)

                                       caaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCtt                                                                                                                                                                                                                                    <  1:1995970/193‑1 (MQ=255)

                                              cacGGTGGAGGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGGTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCa                                                                                                                                                                               >  1:896098/1‑239 (MQ=255)

                                               acGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTTGTTGGTTTATAGACACCCGACTCTTCGGCCACCTTGGCCAt                                                                                                                                                                              >  1:263964/1‑239 (MQ=255)

                                                  gTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCAGAAAGGCATTGTGCCT‑GTGCCAGGGGTTGCTGTGATATAGAAGACGAAAGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGATTCGTTGCTTTATACACACCCGCCTCTTCGGTCACTTTGGACATTgc                                                                                                                                                                           >  1:811039/1‑239 (MQ=255)

                                                   tGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAA‑AGAAAATCTTTAGCGGATGTTTTGTTGCTTTTTATACACCCGCCTCTTTCGCCACTTTGGGCAttttc                                                                                                                                                                          >  2:879057/1‑236 (MQ=255)

                                                     gaaaaaaaGAGATCGGCCCCACAGACAACAAAAAGAACCCGCCCC‑AGAGAGAAGAAAACGCCACCACCCTTTTTC‑GCCA‑GGCGGAAGGGCTTTGTGCGT‑GTGCGAGGGGTGGGTGTAATAAAGAAGAGAAAGGCGATGGAGAAAAAACCCCC‑GGCGTA‑AA‑CCGCAAGAT‑AAAAATTTTGAAGGGGTTGTTTCGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTgcag                                                                                                                                                                         <  2:350451/233‑1 (MQ=255)

                                                          aagaagaTCGGCCCCGAAGACAAAAC‑AAGAAACCGCCCCAAGGGAGAAGCAAAGGCCGCCACCAGGTTTT‑GCCG‑GGGAGAGGGGAGTTGTCCGT‑GTGCCTGGGGTTGGTTGAATAAAGAAGACAAATGAGATTAAGAGGAAACCCCG‑GGCTGT‑AA‑TCGCAAAAA‑AGAAATTTTAAGGCGTTTTTTTCTTTGTTTAATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                     <  2:2143744/233‑1 (MQ=255)

                                                            agagATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGG‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGt                                                                                                                                                                 >  1:306064/1‑239 (MQ=255)

                                                             gagaTCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAg                                                                                                                                                                                                                                 <  2:365402/174‑1 (MQ=255)

                                                             gagaTCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACAACCGCCTCTTCGGCCACCTTGGCCATTGCAGCAGGAAGTa                                                                                                                                                                >  1:516350/1‑239 (MQ=255)

                                                             gagaTCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAg                                                                                                                                                                                                                                 >  1:365402/1‑174 (MQ=255)

                                                               gaTCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaa                                                                                                                                                              >  1:810950/1‑239 (MQ=255)

                                                               acgcGGCCCCGCAAACAACAAAAAGACTGCGCCAG‑AGAGAAAAACAAACGCCGCCAACCAGTTTT‑GCCA‑TGCAGAAGGGCTTTGTGTCT‑GGGCCAGGGGTTGGTGTGATAAAAAAGAAGAATGCGATGGA‑AAGAAAACCCCGGGCGTT‑AA‑CCGCCAGAT‑AAAAATTCTTAAGTTGATGTTTCTTTGTTTTATATACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                               <  2:1321571/235‑1 (MQ=255)

                                                                atggcccccacAGAAAACAAAAAAACGCCGCCCA‑GGAGAGAAGCAAAGCCCGCAAACCAGTTTT‑CCCA‑GGCGGAGGGACTTGGTGCTT‑GTGCGGGGGGTGGCTGAAATAAAAAGGACAAATGCGTTGGATATAAAACCCCG‑GGCGGT‑AA‑CCGCCAGAT‑AGAAATTTTAAAGCGGTTGTTTGGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                             <  2:818228/229‑1 (MQ=255)

                                                                aTCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGGAAATCTTAAGCGGGTGTTTCGTTGCTTTATAGACACCCCCCTCTTCGGCCACTTTGGCCATTTTCGCAGGGAGTaaaa                                                                                                                                                             >  2:67597/1‑239 (MQ=255)

                                                                  cGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TTGCCAAAT‑AGAAAGACTTAAGCGGATGGTTAGTTGCTTTATAGACACCCGCCTCCTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGa                                                                                                                                                           >  1:126463/1‑239 (MQ=255)

                                                                     cTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGGTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGGAGTAAAAGATCa                                                                                                                                                        >  1:2111233/1‑239 (MQ=255)

                                                                       ccGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATACCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGCGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGCTTGAGATGACAACACC‑GGCGGT‑AA‑TCCCCAGAT‑AGAAATGATTAAGCGGATGTTTCGCTGCTTTATAGACACCCCACACTTCGGCCACTCTTGCCATTGCTGCAGAAAAAAAAAGATCaaa                                                                                                                                                      >  2:1708303/1‑239 (MQ=255)

                                                                        cGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  1:1406854/174‑1 (MQ=255)

                                                                        cGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      >  2:1406854/1‑174 (MQ=255)

                                                                          cAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      <  2:876227/172‑1 (MQ=255)

                                                                          cAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGtt                                                                                                                                                                                                                    <  2:1262675/174‑1 (MQ=255)

                                                                          cAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGtt                                                                                                                                                                                                                    >  1:1262675/1‑174 (MQ=255)

                                                                            aaccaaaaaaaGAATCACCCGA‑GGAAAGAAGCAACTGCCGCCAACCAGTTTG‑GCCA‑GGCGGAGGGGCA‑TGTGGCTGGGGCGGGTGGTAGTTGTAAAAAAAAAGAAGAAGGTGAGTGAGAGAAAAACACG‑GGCGGT‑AC‑ACGCAAAAT‑AAAAATTAGTAATGGTATGTTTCTTTGTTTAAAAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGt                                                                                                                                                 <  2:1112658/231‑1 (MQ=255)

                                                                                  acaAAGAATCAGCCCC‑AGTGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGAGCCT‑GCGCCAGTGGCTGCTGTAATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TAGTCAGAT‑AGGAATGCTTAAGCGGATGTTTAGGTCATCTATTGAAACCCGCCTCCTCTGCCACTTTTGCCTTTGCAGCAGGAAGTAAAAGATCAAAAGGGTTgcaa                                                                                                                                            >  1:2060034/1‑235 (MQ=255)

                                                                                      aaaaTCAGCCCA‑AGAG‑GAAAAAAAAGCCGGCCAACAGTTTTAGACA‑GGCGGAAGGGATTGGTGCCT‑GGGCCGGGGTTGGTTGTAATAAAAAAGAAGAAGGAGATTGAGAAAAAAACCCG‑GGGGGT‑AC‑GCGCAAGAT‑AGAAGTTATTAGGCGGTTGTTTGGTTGATTTATGGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCttt                                                                                                                                      <  2:1815867/237‑1 (MQ=255)

                                                                                         aTCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGACTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTtac                                                                                                                                   >  1:985197/1‑239 (MQ=255)

                                                                                         aTCAGCCCC‑AGAGAGAAGCAAATGCCGCAAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAAGGGTGT‑CAGCTTTcac                                                                                                                                   >  1:1476213/1‑239 (MQ=255)

                                                                                           cAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCg                                                                                                                                                                                                                      >  2:613989/1‑155 (MQ=255)

                                                                                           cAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTcaa                                                                                                                                   >  1:872039/1‑236 (MQ=255)

                                                                                            aGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTaa                                                                                                                                                                                                                                  <  1:488664/142‑1 (MQ=255)

                                                                                            aGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTaa                                                                                                                                                                                                                                  >  2:488664/1‑142 (MQ=255)

                                                                                            aGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACt                                                                                                                                >  1:895126/1‑239 (MQ=255)

                                                                                              ccca‑AGAGAGAAGCAAATGCCGCCAACCATTTTT‑GCCA‑TGCGGAAGGGAATTGTGCCT‑GGGCCAGTGGTTGCTGTAATATAGAAGACGAATGGGATTGAGATAAAAACCCC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTAAAGCGGATGTTTCGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                             <  1:1127133/209‑1 (MQ=255)

                                                                                                   agagagAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGGGGATGATTCGTTGGTTTATAAAAACCCGCCTCTTCGGCCACTTTTGCCATTGCAGCAGGAAGTAAAAAATAAAAAAGGTTGT‑CAGCTTTCACACCCActc                                                                                                                           >  1:573484/1‑238 (MQ=255)

                                                                                                   agagagAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGAAGGTTCGTTGCTTTATAGACACCCGCCTCTGCGGCCACTTTGGCCATTGCAGTAAGAAGTAAAAAAACAAAAGGGTTGT‑CAGGTTTCACACCAAccg                                                                                                                           >  2:1459716/1‑236 (MQ=255)

                                                                                                      gagcgaaaaaTGCC‑CCCACCAGTTTT‑GCCAGTGCCGAGGGGCTTGGTGCGT‑GTGCCGGGGTTTGTTGTAATAAAGAAGACAAATGCGATTGAGATAAAACCCCC‑GGCGTT‑AA‑CCGCCAGAT‑AGAATGTCTTAAGCGTTTTTTTTTTTTTTTTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAActctct                                                                                                                        <  2:573484/231‑1 (MQ=255)

                                                                                                         aaGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGAGGAGGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCttt                                                                                                                    >  1:1459313/1‑239 (MQ=255)

                                                                                                         aaGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGG‑AA‑TCGCCAGAT‑AGATAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCCGGAAGTAAAAGATCAAAAGGGTTGC‑TAGCTTTCACACTAACTCTCTCttt                                                                                                                    >  1:951485/1‑239 (MQ=255)

                                                                                                         aaGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGG‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCCAGAAGTAAAAAATCAAAAGGGGTGT‑CAGCGTTCCCACTAAATCTCTCttt                                                                                                                    >  2:980785/1‑239 (MQ=255)

                                                                                                         aaGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATG‑AAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTtta                                                                                                                   >  1:847630/1‑239 (MQ=255)

                                                                                                              aaTGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑ACGCCAGAT‑AGAAAAGCTTAAGCGGATGTTTTGTTGCTTTATAGACAACCGCCACTTCGGCCACTTTGGCCATTGCCGCAGGAAGTAAAAAATCAAAAGGGTTGT‑CAGATTTCAAACTAACTCTCTCTTTATTaa                                                                                                               >  1:1675979/1‑239 (MQ=255)

                                                                                                               acccccccAACCGGTTTG‑GCCA‑TGGGGAGGGGGTTTGTGCCT‑GTGCGAGTGGTTGTGGTAATATAGAAAACGAATGCGATTGAAAT‑AAAACCCC‑GGGGGG‑AA‑TCCCCCGGTAAGAAAGTCTGAAGGGTATGTTTCTTTGTTATATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAg                                                                                                              <  2:263964/233‑1 (MQ=255)

                                                                                                                  ccgaaaacgtttttt‑GCAA‑GGCGAAAGGGTTTTGTGCGT‑GGGCCGGGGGTTGCTGTAATATAGAAGACAAATGCGTTTGAGAT‑AAAACCCC‑GGGGGTAAA‑TCCCAAGAG‑AGAAAGTTTAAAGGGGTTTTTTTGTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCg                                                                                                           <  2:1476213/228‑1 (MQ=255)

                                                                                                                       aacctttttt‑GCCG‑TGCAGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTAATATAGAAAACGAATGCGATTGAGATGAAAACCCC‑GGCGTT‑AA‑TCGCAAGAA‑AGAAAGTCTTAAGCGGATTTTTCGTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  1:1488756/233‑1 (MQ=255)

                                                                                                                            ttttt‑GCCA‑GGCGGAGGGGTTTTGTGCGT‑GTGCCGGTGGTGGTTGTGATAAAGAAGAGAAATGCGATGGAGATAAAACCCCC‑GGCGGA‑AA‑TCGCAAGAT‑AGAAAGTTTAAGGGGGTTTTTTCGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGAt                                                                                                 <  2:895126/238‑1 (MQ=255)

                                                                                                                               tt‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACt                                                                                              >  1:1338239/1‑239 (MQ=255)

                                                                                                                                   ccA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGGCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAAc                                                                                           >  1:881277/1‑239 (MQ=255)

                                                                                                                                    cA‑TGCAGAAGGGTATTGTGCCT‑GTGCAGGTGGTTGGTGTGATATAGAAGACGAATGCGATTGAGATGAAAACCCC‑GGCGGT‑AA‑TCGCCAGAG‑AGAAAGTCTTAAGCGGTTTTTTCGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:1235224/239‑1 (MQ=255)

                                                                                                                                     cgtGCGGAGGGGCATTGTGCGT‑GGGCCAGGGGTTGTTGGAATATAGAAGACGGATGCGTTTGAGAGGAAACCCCG‑GGCGAT‑AA‑CCGCCAGAA‑AGAAAGTCTTAAGCGGTTGTTTCGTTGTTTAAAAACCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  2:222894/237‑1 (MQ=255)

                                                                                                                                         gggAAGGGCTTTGTGCTT‑GTGCCGGGGGTTACTGTAATATAAAAGAGGATTGCGATGGA‑AAGAAAACCCC‑GGGGGTAAA‑GCGCCAAAT‑GAAAAGTTTAAGGGGGTTTTTTGGTTGTTTTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:1338239/236‑1 (MQ=255)

                                                                                                                                         ccGAGGGGCTTTGCGCTT‑GGGCGAGTGGTGGCTGTGATAAAAAAGAGAAAGGCGATGGAGA‑AAAAACCCC‑GGGGGG‑AACTCGCGAAAT‑AAAAATTTTTAGGGGTTTTTTTTTTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACaa                                                                                         <  2:872039/236‑1 (MQ=255)

                                                                                                                                                   ttGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGG‑AA‑TCGCCAGAT‑AGAAAGACTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAATTCGGCGACGAGATACTAACAAAGCATTATAGATg                                                                            >  1:635199/1‑239 (MQ=255)

                                                                                                                                                    tgtgcct‑gtgccAGTGGTTGTTGTGATATAGAAGAAGAATGCGATTAAGATGAAACCCCC‑GGCGTT‑AA‑TCGCCAGAA‑AGAAAGTCTTAAGCGGATGTTTCGTTTCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           <  1:879057/239‑1 (MQ=255)

                                                                                                                                                        cct‑gtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCCGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTTCAGCAGGAAGTAAAAGATCAAAAAGGGTGT‑CAGCTTTCACACTAACTATCTCTTTATTAAATCGGCGACGAGATACCAAAAAAAAATTATAATTGAGaaa                                                                       >  2:553034/1‑239 (MQ=255)

                                                                                                                                                         ct‑gtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAt                                                                      >  1:1555847/1‑239 (MQ=255)

                                                                                                                                                         ct‑gtgcaTGTGGTTGTGGGGAAAAAAAAGAAAAATGGGTTTGAGAAAAAAACCCG‑GGGGGT‑AA‑CCGCAAGAG‑AGAAAGTTTAAAGGGTTTGTTTCTTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  2:32473/238‑1 (MQ=255)

                                                                                                                                                         ct‑gggccAGTGGTGGCTGTAATATAGAAAAAGAAGGCGATTGAGATAAAAACCCC‑GGCGGT‑AA‑CCGCCAGAT‑AAAAATTCTAAAGCGGTTGTTTCTTTGTTTTAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  1:67597/238‑1 (MQ=255)

                                                                                                                                                            gtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGAATTATAGAt                                                                             >  1:2157378/1‑230 (MQ=255)

                                                                                                                                                            gtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGACTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGACACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTg                                                                    >  1:794021/1‑239 (MQ=255)

                                                                                                                                                                 aGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                               >  1:1129454/1‑239 (MQ=255)

                                                                                                                                                                 aGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                               >  1:206767/1‑239 (MQ=255)

                                                                                                                                                                         agatgATATAGAGGACAAATGGTATGAAGAAGAAAACCCG‑GTGGGT‑AA‑TCGCAAGAT‑AAAAATTCTAAAGCGTGTTTTTTTTTGTTTAAAAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCatat                                                       <  2:179917/236‑1 (MQ=255)

                                                                                                                                                                             gATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGGTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGAAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGGCGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCata                                                        >  1:1721418/1‑234 (MQ=255)

                                                                                                                                                                              ataaAGAAGACAAATGAGATGGAAAAAAAAACCCG‑GGCGGT‑AA‑CCGCCAGAT‑AGAAATTCTAAAGAGGTTGTTTCTTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                  <  2:1129454/239‑1 (MQ=255)

                                                                                                                                                                               tataGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGGTGTTTCGTTGCTTTATAGAAACCCGCCTCTTCGGCCACTTTGGCCATTGGAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGACTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAATGATATAGATCATAtc                                                      >  1:332641/1‑234 (MQ=255)

                                                                                                                                                                                    aagaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACCCTAACTCTCTCTTTATTAAGACGGCGACGAGATACTAACAAAGCATTATAGATGAGGAATTGAtataat                                                             >  1:1139956/1‑220 (MQ=255)

                                                                                                                                                                                      gaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGCTACTAACAAAg                                                                                       >  1:425250/1‑194 (MQ=255)

                                                                                                                                                                                      gaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            >  1:1401723/1‑189 (MQ=255)

                                                                                                                                                                                      gaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATta                                                                                  >  1:235702/1‑199 (MQ=255)

                                                                                                                                                                                      gaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGACATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGACGGCGAAGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGACTGGCTta                                          >  1:2226411/1‑239 (MQ=255)

                                                                                                                                                                                      gaCGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TAGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          >  1:323114/1‑239 (MQ=255)

                                                                                                                                                                                        gaaattctaTTGAAATCAAAACACC‑GGCGAT‑AC‑TCGCAAAAG‑AGAATTTTTTAAGGGTATTTTTTGGTGTTTTAAAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          <  2:323114/229‑1 (MQ=255)

                                                                                                                                                                                        cGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TTGCCAGAT‑AGAAAGTCTTAAGCGGGTGTTTCGTTTCTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGATAAATTGAtata                                                               >  1:1083931/1‑216 (MQ=255)

                                                                                                                                                                                        cGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGAAGTTTCGTTGCTTTATAAACACCCGCCTCTTCCGCCACTTTGGGCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAg                                                                                                              >  1:261058/1‑169 (MQ=255)

                                                                                                                                                                                        cGAATGAGATTGAGATGAAAACACC‑GGCGGA‑AA‑TCGCCAGAT‑AGAAAGGCTTAAGCGGATGTTTCGGTGCTTTATAGAAAACCGCCCCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAAGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                            >  1:892034/1‑219 (MQ=255)

                                                                                                                                                                                         gAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAA‑AGAAAGACTTAAGCGGGAGTTTCGTTGCTTTATAGACACCCGCCGCTTCGGCCACTTTGGCCATTGTAGCAGGAAGGAAAAGATCAAAAGGGTTGTACAG‑TTTCACACTAACTCTCTCTTTATTaa                                                                                                               >  2:172443/1‑167 (MQ=255)

                                                                                                                                                                                           aTGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTTGTTGCTTTATAGACACCCGCCTCTTTGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAgg                                      >  1:2051245/1‑238 (MQ=255)

                                                                                                                                                                                           aTGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTTTAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       >  1:103155/1‑189 (MQ=255)

                                                                                                                                                                                           aTGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGGTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCa                                                          >  1:1997517/1‑218 (MQ=255)

                                                                                                                                                                                                       tgaaacccgc‑ggcggT‑AA‑GCGCAAGAA‑AAAAAGCTTTAAGTGGTTTTTTTTTTTTTTTAAAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:2111233/230‑1 (MQ=255)

                                                                                                                                                                                                       taaaaccccc‑GGCGGT‑AT‑CCCCAATAT‑AGAAATTGTAAGGTGGATTTTTCGTGTCTTTTAACCCCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:784344/231‑1 (MQ=255)

                                                                                                                                                                                                               ccgggcgtT‑AC‑TCGCCAGAT‑AGATAGTATTAAGGGGTTGTTTCTTTTCTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGtttt                  <  1:1347233/236‑1 (MQ=255)

                                                                                                                                                                                                                c‑ggcggT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           >  1:653307/1‑180 (MQ=255)

                                                                                                                                                                                                                   gcggT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACACGCCTCTTCGGCCACTTTGGCCATTGCAGCCGGAAGAAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATATGCCCGTAACTCGCATGGTTTTTAt               >  1:2132854/1‑238 (MQ=255)

                                                                                                                                                                                                                                 agtt‑AGAAGGTCTGAGGGGGATTTTTCTTTGTTTAATAAACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  2:810950/236‑1 (MQ=255)

ACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCC‑AGAGAGAAGCAAATGCCGCCAACCAGTTTT‑GCCA‑TGCCGAAGGGCATTGTGCCT‑GTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGT‑AA‑TCGCCAGAT‑AGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGT‑CAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954561‑955017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`