BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	1,321,828	A→C	11.7%	V424G (GTT→GGT)	trpD ←	bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,321,828	0	A	C	11.7%	345.3 / 2.5	127	V424G (GTT→GGT)	trpD	bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
Reads supporting (aligned to +/- strand): ref base A (68/39); new base C (2/16); total (70/57)
Fisher's exact test for biased strand distribution p-value = 4.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCTTCATGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACA  >  NC_007779/1321591‑1322063

tCTTCATGGCCATGCAGGCGCATTAACATGTCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAAc                                                                                                                                                                                                                                             <  1:1243300/239‑1 (MQ=255)

tCTTCATGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAAc                                                                                                                                                                                                                                             <  2:389674/239‑1 (MQ=255)

   tCATGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTc                                                                                                                                                                                                                                          >  2:1432367/1‑239 (MQ=255)

    cATGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTca                                                                                                                                                                                                                                         >  1:720380/1‑239 (MQ=255)

      tGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTca‑tc                                                                                                                                                                                                                                      <  1:2021347/239‑1 (MQ=255)

         ccATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTca‑tcca                                                                                                                                                                                                                                    >  2:726495/1‑238 (MQ=255)

           aTGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCTTTTA‑CCTTGTACCAAACGTGTGAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCGGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCa‑tccatcc                                                                                                                                                                                                                                 <  1:808307/239‑1 (MQ=255)

            tGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACt                                                                                                                                                                                                                                            <  1:421261/229‑1 (MQ=255)

            tGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACt                                                                                                                                                                                                                                            >  2:421261/1‑229 (MQ=255)

            tGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATccc                                                                                                                                                                                                                                >  2:1354495/1‑239 (MQ=255)

            tGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGCCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATccc                                                                                                                                                                                                                                >  1:1731495/1‑239 (MQ=255)

            tGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGGGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATccc                                                                                                                                                                                                                                >  1:1063101/1‑239 (MQ=255)

                 gcgcATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCGCGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCccgccg                                                                                                                                                                                                                            <  1:1278045/238‑1 (MQ=255)

                 gcgcATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCccgcc                                                                                                                                                                                                                             >  1:525371/1‑237 (MQ=255)

                           aTGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGcacca                                                                                                                                                                                                                  >  1:313238/1‑238 (MQ=255)

                               cGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGTTGTGCACCAccgc                                                                                                                                                                                                              >  1:1848145/1‑238 (MQ=255)

                               cGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGATGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTCAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGAGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCAccgc                                                                                                                                                                                                              >  1:1795501/1‑238 (MQ=255)

                                  cGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACcgccgc                                                                                                                                                                                                           >  1:634854/1‑238 (MQ=255)

                                    ttCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCcgcgcg                                                                                                                                                                                                        >  1:431809/1‑239 (MQ=255)

                                      cGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGtt                                                                                                                                                                                                      >  2:1735521/1‑239 (MQ=255)

                                             aCGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATAcc                                                                                                                                                                                                >  1:610052/1‑238 (MQ=255)

                                                           ggcggcGCCGCCTTTC‑CCTTGTAACAAACGTGTTAAAATGTCAGGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAg                                                                                                                                                                                  <  1:1238363/238‑1 (MQ=255)

                                                               gcgTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTc                                                                                                                                                                             >  1:990332/1‑239 (MQ=255)

                                                               acaccGCTTTTC‑CTTTGAAACAAACGTTTAAAATTGTCCCGGTTTTTTT‑CCGG‑TGTC‑CCGCCTGCCATTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGTTTTTAATTTCGCCGTCATGCGGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAACCTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGttt                                                                                                                                                                              <  2:2065301/234‑1 (MQ=255)

                                                                cgTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCg                                                                                                                                                                            >  1:772266/1‑239 (MQ=255)

                                                                     cttttC‑CCTTGTAAAAAACTTTTTAAAAGTTCCGGGTTTTCTT‑CCGG‑GGTC‑CCGCCTGCCGGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAAt                                                                                                                                                                       <  2:185735/237‑1 (MQ=255)

                                                                               tcaaaaaaaCGTTTGAAAATGCCCGGGTTTTTTT‑CGGT‑TTTC‑CCGCCT‑CCAGGTGGTCCTTGTTGGGAGGTTGTACGGCCAAGGTCTGCGGGGGGGGGCTGATATTTTTAATTTTCGCCGTCATGCAGTG‑CGGCACCGATTGGCGGCGCGTGTAATGAATCTCCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCAc                                                                                                                                                              <  2:2108029/233‑1 (MQ=255)

                                                                               taggagacggttgtTAAAGTTTCTGGTTTTTCTC‑CCGT‑TGTT‑CCGCCGGCCAGTTGCTCT‑GGGTGGTAGGGTGCAAGCCCAAAGTCTTCGGCGGTGAGCTGTTGGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAACCTTCA‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCAc                                                                                                                                                              <  2:476947/228‑1 (MQ=255)

                                                                                ttAAAAAACGTTTTGAAATGTTCGGGTTTTTTTCCCGG‑TTTC‑CCCCCTGCCGTTTGTTCC‑GGGTGGTAGGTTTTCGGGCCAAGTTCTTCGGCGGTGGGCTGAGAGTTTTTAATTTCCCCGTCAGGCAGTC‑CGGCAACTATTGTCGGCGCGTGTAATGAAACTTCA‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCAc                                                                                                                                                              <  2:1245662/238‑1 (MQ=255)

                                                                                 tAACAAACGTGTTAACATGTCACGGTTTTCTC‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGCTAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAACCTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCACAGCACGCGCAAGGTTTCGGCAATCGGCAGCAc                                                                                                                                                              >  1:1872239/1‑237 (MQ=255)

                                                                                      aaCGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTTAGCTGATAGCTTTTAATTTCGACGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGca                                                                                                                                                                                                                     >  1:453063/1‑177 (MQ=255)

                                                                                       aCGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CTGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCg                                                                                                                                                      >  2:599698/1‑239 (MQ=255)

                                                                                           gtTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACt                                                                                                                                                  >  1:561873/1‑239 (MQ=255)

                                                                                            ttAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGCTTCGGCCATCGGCAGCACCAGTTCCGGACta                                                                                                                                                 >  1:1437864/1‑239 (MQ=255)

                                                                                             tAAAATGTCCCGGTTTGCTT‑CCGG‑TCGT‑CCGCCGGCCGTTGGCTCC‑GGGTGTTAGGTGTTCAGGCAAAAGTCTTCGGCGGGGGGCTGATGGTTTTTAATTCCGCCTTCATCCGGTT‑CGGCAACGATTGCCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACtat                                                                                                                                                <  2:2228490/239‑1 (MQ=255)

                                                                                                aaTGTCTGGGTTTT‑TT‑TCGG‑TGTTGCCGCCTGCCAGTTGGTCC‑TGGTGGAAGGGGTTCAGGCCAAAGTCTTCTGGGGTGAGCGGATAGTTTTTAATTTCGCCGTCATGCAGTT‑CGGCACCGTTTGTCGGCGGGTGTAATGAAACTTCA‑TCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATaaa                                                                                                                                             <  2:938185/239‑1 (MQ=255)

                                                                                                    gtgcGGTTTTTCT‑CCGGTTGTC‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCACCGATTGTCGGCGCGTGTAATGAAACTTCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAacac                                                                                                                                          <  2:634854/236‑1 (MQ=255)

                                                                                                      aCGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTTCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGACACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCaa                                                                                                                                       >  1:892511/1‑239 (MQ=255)

                                                                                                      aCGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCaa                                                                                                                                       >  1:1396603/1‑239 (MQ=255)

                                                                                                                     g‑tgtT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAaca                                                                                                                                           >  1:221886/1‑221 (MQ=255)

                                                                                                                     g‑tgtT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAgcggc                                                                                                                         >  1:174553/1‑239 (MQ=255)

                                                                                                                         tt‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGa                                                                                                                      >  1:1990256/1‑239 (MQ=255)

                                                                                                                            ccGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACCCCAGcggcggga                                                                                                                     >  2:683403/1‑236 (MQ=255)

                                                                                                                              gcgggccgtgtGCTCC‑TGGTGGTGGGGTGTCGGGCCAAATTCTTCTGCGGGGAGCTTGTAGCTTTTAATTCCGCCGTCAGGCATTT‑CGGAAACGTTTGTCGGCGCGTGTAATGAACCTTCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  <  2:1848145/229‑1 (MQ=255)

                                                                                                                              gccccccggTTGCCTC‑TGGGGGTAGGGTGTCAGCCCAAAGTTTTCTGCGGGGAGCTGTTATCTTTAATTTCCCCCGTCATGCAGTC‑CGGCAACTTTTGCCGGCGCGTGTAATGAACCTTCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  <  2:313238/231‑1 (MQ=255)

                                                                                                                              gCCTGCCATTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGGGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  <  1:1707547/239‑1 (MQ=255)

                                                                                                                              cccTGCCATGTGCTCC‑GGTTGGTAGGGTGCCAGGCCATAGTCTTGTGCGGTGAGCTGAGAGTTTTGAATTGCCCCTTCTGGCGTTT‑CGGCACGGTTTGTCGGCGCGTGAAATG‑AACTTCACTCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  <  2:1504942/238‑1 (MQ=255)

                                                                                                                                       ttGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGACGCCCCCGGGTaa                                                                                                         >  2:1159537/1‑239 (MQ=255)

                                                                                                                                          cTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAg                                                                                                                                                                                            <  1:318379/153‑1 (MQ=255)

                                                                                                                                          cTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAg                                                                                                                                                                                            >  2:318379/1‑153 (MQ=255)

                                                                                                                                            cc‑TGGGGGTGGGGTGTCAGCCCAAGGTCTTCTGGGGTTGGTGGTTAGTTTTTATTTGCCCCGTCAGGCGGTT‑CGGCAACGTTTGCCGGCGCGTGTAAGAAAACTTCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAAt                                                                                                    <  2:772266/239‑1 (MQ=255)

                                                                                                                                                 gtggtAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCg                                                                                                               <  1:1110107/224‑1 (MQ=255)

                                                                                                                                                 gtggtAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCg                                                                                                               >  2:1110107/1‑224 (MQ=255)

                                                                                                                                                     tggtggttagggCAAAAGTCTCCTGCGGTGGGCGAATGTCTTTTATTTCCGCCGTCAGCCATTT‑CGGCACCGTTGGCCGGCGCGTGTAATGAATCTTCC‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCa                                                                                             <  2:485340/228‑1 (MQ=255)

                                                                                                                                                     tatggttgcAGCCCAATGTTTTCGGCGTTGGGTTGAGAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGAACCGATGGCGGGCGCGTGTAATGAACCTTCA‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCa                                                                                             <  2:174553/230‑1 (MQ=255)

                                                                                                                                                     tAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCa                                                                                             <  1:599698/238‑1 (MQ=255)

                                                                                                                                                     tAGGGTGTCAGGCCAAAGTCTTCTGCGGCGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCCGGTTAAGCAATGGGCCCa                                                                                             >  1:1758100/1‑238 (MQ=255)

                                                                                                                                                      ggggTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTCCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGc                                                                                           <  1:80040/238‑1 (MQ=255)

                                                                                                                                                      aGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGACGCGCCGGGGTCATCAAAGGccccca                                                                                            >  2:2108794/1‑236 (MQ=255)

                                                                                                                                                        ggTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  >  2:907319/1‑214 (MQ=255)

                                                                                                                                                        ggTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATgcg                                                                                                                  <  1:907319/214‑1 (MQ=255)

                                                                                                                                                          tgtCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAaca                                                                                                                                           >  1:94356/1‑187 (MQ=255)

                                                                                                                                                          tgtCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGACGGCCCGGGCTAATCAATGGCCCCAGCCCAt                                                                                       >  2:2035278/1‑239 (MQ=255)

                                                                                                                                                           gtCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCAGTAGCCCCGGGTTAATTAACTGCCCCCGcacaat                                                                                      >  2:1531403/1‑237 (MQ=255)

                                                                                                                                                            tCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGa                                                                                                                                                    >  1:1853972/1‑176 (MQ=255)

                                                                                                                                                            tCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGGTAATCAATGGCCCCAAcacaatt                                                                                     >  2:716199/1‑236 (MQ=255)

                                                                                                                                                              gggCCAAGGTCTTCTGCGGTGAGCGTATAGCTTTTAATTTCGCCGTCATGCAGTC‑CGGCACCGATGGTCGGGGCGTGTAATGAACCTCCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGaa                                                                                   <  2:1219765/238‑1 (MQ=255)

                                                                                                                                                              gggCAAAGGTCTTGTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTC‑CGCCACCGTTTGTCGGCGCGTGAAATGAACCTTCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGaa                                                                                   <  2:799699/238‑1 (MQ=255)

                                                                                                                                                                gCCAAAGTCTACTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGACGCGCCGGGATCATCAATGGCCCCAACACAATGAACa                                                                                 >  1:2051487/1‑239 (MQ=255)

                                                                                                                                                                 ccAAAGTTTTCTGGGGTGGGCTGATAGCTTTTAATTTCGCCGTCATGCATTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGaa                                                                                   <  2:610052/236‑1 (MQ=255)

                                                                                                                                                                 ataaaGTTTTTTGCGGTGGGATGAAGGCTTTTAATTCCGCCGTCA‑GCAGTTCCGGCAAGGATGGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGaa                                                                                   <  1:2108794/234‑1 (MQ=255)

                                                                                                                                                                     aGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTg                                                                            <  1:2107564/239‑1 (MQ=255)

                                                                                                                                                                     aGTCTTCGGCGGGGTGTTGATAGCTTTTAATTTCGCCGTCATGCAGTC‑CGGCACGGATTGTCGGGGCGTGTAATGAACCTCCC‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTg                                                                            <  2:1063101/239‑1 (MQ=255)

                                                                                                                                                                          tctGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGGCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGGGCgggg                                                                       >  1:1804117/1‑238 (MQ=255)

                                                                                                                                                                          tctGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGGTAATCAAATGCCCCAGCCCATTGAACCGGGTGCGGGt                                                                       >  2:1112676/1‑239 (MQ=255)

                                                                                                                                                                            tGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGGTAATCAAAGGCCCCAGCACATTGAACAGGGGGCGGGgtt                                                                     >  1:1651534/1‑239 (MQ=255)

                                                                                                                                                                                 tGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACtata                                                                                                                                               >  1:34360/1‑160 (MQ=255)

                                                                                                                                                                                 tGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAacac                                                                                                                                          >  2:1622766/1‑165 (MQ=255)

                                                                                                                                                                                 tGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAacac                                                                                                                                          <  1:1622766/165‑1 (MQ=255)

                                                                                                                                                                                 tGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAac                                                                                                                                            >  2:1877746/1‑163 (MQ=255)

                                                                                                                                                                                 tGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAac                                                                                                                                            <  1:1877746/163‑1 (MQ=255)

                                                                                                                                                                                  gggcctaaatcTTTTAATTTCGCCGTCATGCATTC‑CGGCAACGCTTGTCGGCGCGTGTAATGAAACTCCA‑TCCATCCCGCCGCTGTGCCCCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGt                                                                <  1:233143/228‑1 (MQ=255)

                                                                                                                                                                                               ttAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGGTAATCAATGGCCCCAGCACATTGAACAGGGGGCGGGGTTTCCGTTGCTGGCGAACCg                                                   >  1:253523/1‑238 (MQ=255)

                                                                                                                                                                                                tAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCCGCACATTTAACAGGGGGCGGGGTTTCAGTTGCTGGCGGACCgg                                                  >  2:2200744/1‑238 (MQ=255)

                                                                                                                                                                                                      cGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGGAATGAAACTTCA‑TCCATCCCGACGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATcgcg                                           >  1:2084034/1‑239 (MQ=255)

                                                                                                                                                                                                      cGCCGTCATGCAGTT‑CGGCAACGATTGACGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGGAGCGCCGGGGTAATCAATGGCCCCAGCACATTGAAAAGGGGGCGGGGTTTCAGTTGCTGGGGAACCGGCCCcgcg                                           >  2:1081103/1‑239 (MQ=255)

                                                                                                                                                                                                                 aGTC‑CGGCAAGGATGGCGGGGGGGGGTAATAAACCTCCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATc                                 <  2:1660410/238‑1 (MQ=255)

                                                                                                                                                                                                                    tgcGGCACAGATTGTCGGCGCGGGTAATGAAACTTCA‑CCCACCCCGCCGCTGTGCCCCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCg                               <  2:1934035/236‑1 (MQ=255)

                                                                                                                                                                                                                      cgatcgggttggTCGGAGCTGGTAAGAAACCTCCA‑CCCACCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGgtg                            <  2:2051487/228‑1 (MQ=255)

                                                                                                                                                                                                                      cGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGAACATTGGACAGGGTGCGGGGTTTCAGTTGCTGGCGAACCGGCATTGCGTGGGGGAGTACgggg                            >  1:1599999/1‑237 (MQ=255)

                                                                                                                                                                                                                              aTTGTCGGCGCGTGTAATGAACCTTCA‑TCCATCCCGCCGCTGTGCACCACCGCcgcgc                                                                                                                                                                                                         <  2:636523/58‑1 (MQ=255)

                                                                                                                                                                                                                              aTTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCcgcgc                                                                                                                                                                                                         >  1:636523/1‑58 (MQ=255)

                                                                                                                                                                                                                                      ggcgTGAAATGAAACTTCA‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCa             <  1:1112676/237‑1 (MQ=255)

                                                                                                                                                                                                                                        cgTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGGTAATCAATGGCCCCAGCACACTGTACCAGGGGCGGGGTTTCAGGTGCTGGCGAAACGGCCTCCCGTGGGGGAAACCGGGGGGAGAATTTGGCGCaaaa          >  2:1206914/1‑238 (MQ=255)

                                                                                                                                                                                                                                         gtgtAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAag          <  1:1069463/238‑1 (MQ=255)

                                                                                                                                                                                                                                          tgtAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGgc                                                                                                                                                                   >  2:488923/1‑84 (MQ=255)

                                                                                                                                                                                                                                          tgtAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGgc                                                                                                                                                                   <  1:488923/84‑1 (MQ=255)

                                                                                                                                                                                                                                             attGAAACTCCA‑CCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGa      <  1:716199/236‑1 (MQ=255)

                                                                                                                                                                                                                                                gAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACa  <  1:726495/239‑1 (MQ=255)

                                                                                                                                                                                                                                                gAAACTTCA‑CCCCTCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACa  <  1:1206914/239‑1 (MQ=255)

TCTTCATGGCCATGCAGGCGCATTAACATGGCGACGTTCGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTA‑CCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTT‑CCGG‑TGTT‑CCGCCTGCCAGTTGCTCC‑TGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTTTAATTTCGCCGTCATGCAGTT‑CGGCAACGATTGTCGGCGCGTGTAATGAAACTTCA‑TCCATCCCGCCGCTGTGCACCACCGCCGCGCGTTGATACCCCAGCACGCGCAAGGTTTCGGCAATCGGCAGCACCAGTTCCGGACTATAAACACCAATTAACGCCAGCGGCGGATGCGCCGGGTTAATCAATGGCCCCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACA  >  NC_007779/1321591‑1322063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`