BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	1,521,798	G→A	10.6%	intergenic (+20/‑178)	curA → / → Y75_RS07665	NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,521,798	0	G	A	10.6%	311.5 / 8.8	151	intergenic (+20/‑178)	curA/Y75_RS07665	NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
Reads supporting (aligned to +/- strand): ref base G (40/92); new base A (13/3); total (54/97)
Fisher's exact test for biased strand distribution p-value = 1.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACC  >  NC_007779/1521563‑1522033

aaaCGTATGCGCTTGCAAGGTTTTATTATCGCTCAGGATTAGGGTACCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAgccg                                                                                                                                                                                                                                                <  2:2200375/239‑1 (MQ=255)

       ttCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGGTTCAACGGCGGGGTGAGCCG‑CCATaaa                                                                                                                                                                                                                                        >  2:844997/1‑239 (MQ=255)

               cAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGGTTAAAGTTTCAACGGACGGGTGAGACG‑CCATAAAAAAACAgg                                                                                                                                                                                                                                >  2:341268/1‑239 (MQ=255)

                   gTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTCCCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGAAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAATTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCaa                                                                                                                                                                                                                             <  1:844997/238‑1 (MQ=255)

                    ttttATTATCGCTAAGGATTATGGTCACCGCATCCGGGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAACCCACTACCGCGAAAAAATTACGGACGG‑TTTAGA‑GAAGGGCCCAAAGACTTTTATCGGCCTGCTGAAGGGAAAAAAC‑TTGGGCAAAGTGGTGACCCGCGGGGCGGGTGATGTGAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCaaaa                                                                                                                                                                                                                           <  2:730008/239‑1 (MQ=255)

                      ttattaTCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGGCGCGTAAACCG‑CCATAAAAAACCAGGCcaaaaa                                                                                                                                                                                                                         >  2:105523/1‑238 (MQ=255)

                              gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGGGGAAGCCG‑CCAAAAAAAACCAGGGAAAAAGGTTAtttt                                                                                                                                                                                                                 >  1:172428/1‑239 (MQ=255)

                              gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGGGTTAACCG‑CCCAAAAAAACCCGGGAAAAAACTtttttt                                                                                                                                                                                                                 >  2:744655/1‑239 (MQ=255)

                                 cAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAGC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAg                                                                                                                                                                                                              <  1:2012207/239‑1 (MQ=255)

                                  agaaTTATGGCACCCGCACCCATGGGTTTCAGAGGGAGATGGGGCATGGGGTGAAAGAGAAAAAACACCACTACCGCGAAGAAATTACTGAGGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGATAAAAAC‑TGCGGCAAAGGGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAg                                                                                                                                                                                                              <  2:1582697/235‑1 (MQ=255)

                                      ttATGGTAACCCCATCCATGAGTTTCAGAGGGAGAGGGGGCAAGGGGGGAAAGAGGATAAAACCCACTCCCGCGAAGAAATTACTGACGG‑TTAAGA‑GAATGCGCAACAGAGGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGTTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTtta                                                                                                                                                                                                        <  1:341268/239‑1 (MQ=255)

                                         tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGAGGATTAAAGTTTCAACGGCGGGGGGAACCG‑CCCTAAAAAAACAAGGAAAAAGAATATTTTTCG‑TTttaatt                                                                                                                                                                                                     >  2:1675335/1‑239 (MQ=255)

                                         tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGCGGAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTTAGCCTttaat                                                                                                                                                                                                      >  1:1843432/1‑239 (MQ=255)

                                             ccccGCATCCATGAGTTTCAGAGGGGGAGGGGGAAAGGGGGAAAAGAGGATAAAACCCACTACCGCGAAAAAATTACTGAGGG‑TTTAGA‑GAATGCCCCACAGACTTTTATCGGCCTGCGGAAGGGTAAAACC‑TTCGGCAAGGGGGTGATCCGCGTGGCGGGTGATGATAAAAGTTACAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATtaac‑t                                                                                                                                                                                                <  2:1632660/237‑1 (MQ=255)

                                             cACCGAATCCAGGCGTTTCAGAGGGAGAGGGGGCAAGGGGTAAAAAAGGATAAAATCCACTACCGCAAAAAAATTACGGACGT‑TTAAGA‑GAAGGCGCCACAGACGTTTAGCGGCCTGCTGAAGGGAAAAAAC‑TTCGGCAAAGTGGTGACCCGCGTGGCGGGTGATGATAAAATTTTCACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATtaac‑t                                                                                                                                                                                                <  2:1088341/239‑1 (MQ=255)

                                              aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGGTGATTAAAGTTTCAACGGCGGGGGGAACCG‑CCCTAAAAAACCACGGAAAAAACTTTTTTTTAG‑CTTTTATtaac‑ta                                                                                                                                                                                               >  2:2119965/1‑239 (MQ=255)

                                              aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGGGGTAGCCC‑CCCTAAAAAAACAGGCAAAAAAATTATTTTTAG‑ATTTTATtaaa‑ta                                                                                                                                                                                               >  2:1026526/1‑239 (MQ=255)

                                              aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACAGCGGCGTAACCCG‑GCATAAAAATCCCGGCAAAAAGATTATTTTCAG‑CTTTAATtaaa‑ta                                                                                                                                                                                               >  1:1214784/1‑239 (MQ=255)

                                              aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAGTAAAATTTCAACAGCGGAGGAAGCCC‑CCATAAAAAACCAGGGAAAACAATTATATTTAG‑CCTTAATtaac‑ta                                                                                                                                                                                               >  1:2167522/1‑239 (MQ=255)

                                              aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAAACCAGGCAAAAAGCTTATTTTCAG‑CTTTAATttac‑ta                                                                                                                                                                                               >  1:175507/1‑239 (MQ=255)

                                                cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAAAGGCGGCGCAAGCCG‑CCATAAAAATCCAGGCAAAAAGCGTATTTTTAG‑CTTTAATtaac‑taac                                                                                                                                                                                             >  1:1657501/1‑239 (MQ=255)

                                                cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCAGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGGAAGCCC‑GCAAAAAAATCCAGGTAAAAAGCTTATTTTCTA‑CTTTAATtcac‑aaa                                                                                                                                                                                              >  1:1939285/1‑238 (MQ=255)

                                                  cctCCATGAGTTTCAGAGGGAGAGGGGGCAATGGGTGAAAGAGAATAAAACCCACGCCCGCGAAGAAATTACTGACTT‑TTTAGA‑GAATGCGCCACAGAGTTTTAGCGGCCTGTGGAGGGGTAAAAAC‑TGCGGCAAAGGGGTTAGCCGCGTGGCGGGTGAGTATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACca                                                                                                                                                                                           <  1:431158/237‑1 (MQ=255)

                                                   aTCCATGATTTTAAGGGGGAGATGGGGCAATGGGGGAAAGAGAAAAAAATCCACTACCGGAAAAAAATTACTGACGT‑TTAAGA‑GAATGCCCAACAGACTTTTAGGGGCCTGCGGAAGGTAAAAAAC‑TTCGGCAAGGTGGTGATCCGGGTGGCGGGTGATGATAAAAGTTTCAGCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACca                                                                                                                                                                                           <  2:1843432/238‑1 (MQ=255)

                                                   aTCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGGGGTAACCG‑CCCTAAAAATACAGGGAAAAAACCTTTTTTCTC‑GTTTTATTAAC‑TAACcaa                                                                                                                                                                                          >  2:392811/1‑238 (MQ=255)

                                                   aTCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGAAAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACca                                                                                                                                                                                           <  1:845641/238‑1 (MQ=255)

                                                     ccATGAGTTTCAGGGGGAGATGGGGCAAGGGGAGAAAGAGAATAAAACCCTCTACCGAGAAGAAAATACTGAGGG‑TTTAGA‑GAATGCCCAACAGACGTTTAACGGCATGCTGAAGGAAAAAAGC‑TCCGGAAAAGGGGTGAGCCGCGTGGGGGGTGATGATTAAAGTTTAAGGGGCGACAAAACCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCAcat                                                                                                                                                                                        <  2:260714/239‑1 (MQ=255)

                                                     ccATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCAcat                                                                                                                                                                                        <  1:1499672/239‑1 (MQ=255)

                                                               cAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTctg                                                                                                                                                                              <  1:2090325/239‑1 (MQ=255)

                                                               cAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACGACCGCGAATAAATTGCTGACGG‑TTTAGA‑GAATGCGCCGCAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTctg                                                                                                                                                                              <  1:1127027/239‑1 (MQ=255)

                                                               cAGAGGGAGATGGGGAAATGGGTGAAAGAGGATAAAACCCACTACCGCGAAGAAATGACTGACGG‑TGTAGG‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGTTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTctg                                                                                                                                                                              <  1:983272/239‑1 (MQ=255)

                                                                    ggAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGGTTAAAGTTTCAACGGCGGGGGGAGCCGCCCCTAAAATCCCGGGAAAAAAACTTTTTTTC‑G‑CGTTTATTAACATAACCACA‑CCTTGTTTtt‑ctgcc                                                                                                                                                                        >  2:2212268/1‑238 (MQ=255)

                                                                     gaggTGGGGGAAGGGGAGAAAGAGGATAAAATCCACTACAGGGAAGAAATTACTGACGG‑TTAAGA‑GATGGCGCAAGAGAGGTTTGT‑GGCCTGGGGGAGGGGAAAAACGTGCGGCAAGGGGGTGTTCGGGGGGGGGGGGGAGGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTg‑ctgct                                                                                                                                                                        <  2:1361821/238‑1 (MQ=255)

                                                                     gaggTGGGGAAATGGGTAAAAGAGGATAAAATCCACTCCCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTAGCGGCCTGCTGAAGGATAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATAAAATTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAA‑CTTTAATTAAC‑TAACCACATCATTGTTCTg‑ctgct                                                                                                                                                                        <  2:630789/238‑1 (MQ=255)

                                                                        agggggAAATGGGTAAAGAAGAAAAAAAACCACTCCCG‑GAAGAAATTTCCGGAGG‑TTTTGATGAATGCCCCACAGACGTTAACCGGCGTGCGAAAGGGTAAAAAC‑TCCGGCAAAGGGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCtaat                                                                                                                                                                     <  2:1754842/236‑1 (MQ=255)

                                                                        aTGGGGCAATGGGTGAAAGAGGATAAAACCCACTGCCGCGAAGAAATTACGGACGT‑TTTAGA‑GATTGGGCCCCAGACTTTTATGGGCCTGTTGAAGGGTAAAAAC‑TCCGGCAAAGTGGTTATCCGCGGGGCGGGTGATAATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCtaat                                                                                                                                                                     <  1:1689473/238‑1 (MQ=255)

                                                                         gggggAAATGGGTGAAAGAGGATAAAATCCACTCCCGCGAAGAAATTACGGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGTTAAAAAC‑TTCGGCAAAGTGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCtaat                                                                                                                                                                     <  2:1939285/236‑1 (MQ=255)

                                                                            ggCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGT‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAACC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGACGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATaaca                                                                                                                                                                 <  1:158226/238‑1 (MQ=255)

                                                                               aaTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAacaaca                                                                                                                                                              <  1:1109970/238‑1 (MQ=255)

                                                                                aTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGAAA‑CCATAAAAATCCAGGAAAAAAACTTATTTTCAG‑CTTTAATTAAA‑TAACCAAATCATTGTTctg‑ct                                                                                                                                                                           >  1:72763/1‑224 (MQ=255)

                                                                                   ggTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCTCCACAGACTTTTATGGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAATTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGtt                                                                                                                                                                                 <  1:1517774/216‑1 (MQ=255)

                                                                                    gTGAAAGAGGAAAAAATCCTTATCCGCGAAGAATTTACGGAGGG‑TTTAGA‑GAGTGCGCCACGGGTGTTTATCGGCCTGCGGAAGGGTAAAAAC‑TGCGGCAAAGGGGGGATCCGCGGGGCGGGTGATGATTAAAGTTTAAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGt                                                                                                                                                         <  2:2198525/238‑1 (MQ=255)

                                                                                     tGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGGGAGCCG‑CCATAAAAATACAGGCAAAAAACTTATTTTTCG‑CTTTAATTTAC‑CAAACCCATCATTTTTCTG‑CTTCCAATAAAAACaaaagggg                                                                                                                                                       >  2:281012/1‑234 (MQ=255)

                                                                                     agAAAGAGGAAAAAACCCACCACAGCAAAAAAAGT‑GTGAAGGTTTTAGA‑GAATGCGCCACAGCGGTTGAGCGGCTGGTTGAAGGGTAAAAAC‑TTCGGCAAAGGGGGGATCCGCGTGGCGGGTGATAATTAAAGTTTCAACGGGGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTg                                                                                                                                                        <  2:1364885/237‑1 (MQ=255)

                                                                                       aaggagGATAAATCCCACTACCTCAAAGAATTTCCTGACGG‑TTTAGA‑GAAGGCGCCACAGACGTTTAGCGGCGTGCTGAAGGGTAAAACC‑TTCGGAAAAGTGGTAATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAg                                                                                                                                                      <  1:1023089/235‑1 (MQ=255)

                                                                                       aagaagaaaaaaaCCCACTACCGAAAGGAAATTACTGACGG‑TTTAGA‑AAAGGCCCCACGGAGGTTTATCGGCCGGCTGAAGGATAAAACC‑TCCGGAAAGGTGGTGATCCGCGGGGGGGATGAAGATAAAATTTTAACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAg                                                                                                                                                      <  2:1778690/229‑1 (MQ=255)

                                                                                       aaaGAGGAAAAAACCCACTACCGGAAAGAAATTACTGACGG‑TTGAGA‑GAAGGCGCCACAGACGTTTATCGGCCGGCTGAAGGGTAAAACC‑TGCGGAAAAGGGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCACCGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAg                                                                                                                                                      <  2:1553242/238‑1 (MQ=255)

                                                                                             gATAAAATCCACTACCGCGAAGAAATTACTGACGT‑TTTAGA‑GAATGCGCCCCAGACGTTTATCGGCCTGCTGAGGGGTAAATAC‑TTCGGCAAAGTGGTGACCCGTGTGGGGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCtaataa                                                                                                                                                                   <  1:1679176/219‑1 (MQ=255)

                                                                                                aaaaTCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGAAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAATTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGa                                                                                                                                            <  1:879592/239‑1 (MQ=255)

                                                                                                  aaTCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCCGGCAAAAAACTTATTTTCAG‑GTTTAATTAAA‑TAAACACATCATTGTTttg‑ct                                                                                                                                                                           >  1:1303315/1‑206 (MQ=255)

                                                                                                   aTCCAGTCCCGGAAAAAATTTACTGAGGG‑TTTAGA‑GAATGGACAACAGACTTTTATCGGCCTGGTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGCGCCGCGTGGCGGGTGATGATTAAAGTTTCACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGatta                                                                                                                                         <  2:1192169/239‑1 (MQ=255)

                                                                                                    ccccctcccGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTACCGGCTGGCTAAAGGGTAAAAAC‑TTCGGCAAAGGGGTGAGCCGCGTGGGGGGGGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATc                                                                                                                                                                                                                                    <  1:1739864/143‑1 (MQ=255)

                                                                                                     ccactccgtagaagaaATTACGGTGGG‑TTTAAA‑GAATGCACCACAGTTGTTTAGCGGGTTGCTGAAGGGTAAAAAC‑TTCGGAAAAGGGTTGACCCGCGTGGGGGTTGAAGATAAAAGTTACAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGattatt                                                                                                                                       <  2:1576703/229‑1 (MQ=255)

                                                                                                     ccACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGattatt                                                                                                                                       <  1:263485/239‑1 (MQ=255)

                                                                                                     ccACAACCGCAAAGAAATACCGGGCGG‑TTTAGG‑GAATGCCCCACAGACGTTTACCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGTTAATCCGCGTGGGGGGTGATGATTAAAGTTTCAACGGCTGCGTAAGCCG‑CCATAAAAAGCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGattatt                                                                                                                                       <  1:2144984/239‑1 (MQ=255)

                                                                                                        cTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGGTTATTTTTAG‑CTTTAATTTAA‑TAAACAAATCATTg                                                                                                                                                                                   >  1:2190647/1‑193 (MQ=255)

                                                                                                              ggaagaaATTACGGAGGG‑TTAAGA‑GAATGCGCCACAGACTTTTCTCGGCCTGCTGAAGGGTAAAATC‑TTCGGCAAAGTGGTGATCCGCGTGGGGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTaa                                                                                                                               <  2:12240/237‑1 (MQ=255)

                                                                                                              ggaaaaaATTACGGGTGT‑TTAAGA‑AAATGCGCCACAGATGTTTAGCGGCGGGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGGGATCCGCGTGGGGGGTGAGAATAAAAGTTTCACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTaa                                                                                                                               <  2:1792363/237‑1 (MQ=255)

                                                                                                              cgAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGTTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAgccg‑c                                                                                                                                                                                                                                              <  1:1975021/130‑1 (MQ=255)

                                                                                                                   aaattcgtggggg‑TTTAGA‑GAAGGCGCCACGGGTGTTTAGGGGCGTGGAGAAGGGAAAAAAC‑TGCGGAAAAGGGGTGATCCGCGGGGGGGGTGATAATAAAAGTTTAAACGGCGGAGAAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACAcc                                                                                                                         <  2:2133771/228‑1 (MQ=255)

                                                                                                                   aaaTTACTGACGT‑TTTAGA‑GAATGCGCAACAGACGTTTAGCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAGGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCAcat                                                                                                                                                                                        <  1:262102/177‑1 (MQ=255)

                                                                                                                         cTGACGG‑TTTAGA‑GAATGCGCCATAGTCGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGAATAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑a                                                                                                                  <  1:392811/239‑1 (MQ=255)

                                                                                                                                 tttAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTAt                                                                                                                                              <  1:1435625/205‑1 (MQ=255)

                                                                                                                                         aaTGCGCCACAGTCGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGGGTGGCGGGTGATGATTAAAGTTTCACCGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTAtttt                                                                                                                                                                                                                 <  1:1415605/134‑1 (MQ=255)

                                                                                                                                          ctGCGCCACAGCCTTTTACCGGCCTGCTTAAGGGTAAACAC‑TTCGCCAAAGTGGTGGTCCGCGTGGCGGGTTATGATAAAATTGTCAGGGGGGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCAt                                                                                                    <  2:1007167/237‑1 (MQ=255)

                                                                                                                                          aTGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGGTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCTCGGCAAAAAGCTTATTTTTAA‑ATTTAATTAAC‑TAACCACATCAATGTTTTG‑CTGCTAATAACAACCAAAGGGAGTGGTTTTTGATTATTCACTTTAATa                                                                                                                             >  1:1876102/1‑214 (MQ=255)

                                                                                                                                          aTGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGCGGAAGCCG‑CCCTAAAAATCCAGGCAAAAAACTTATTTTTAG‑CTTTTATTAAA‑TAACCACATCATTGTTCTG‑CTTCTAATAAAAACAAAAGTGAGTGGGTTATGATTTTTTACTTTTATAAACCAGGGG‑AAATTCTTCTTCCAt                                                                                                    >  2:1951477/1‑238 (MQ=255)

                                                                                                                                          aTGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGCGGAAGCCG‑CCCTAAAAAAACAGGCAAAAAACTTATTTTTTG‑CTTTTATTAAA‑TAAACACAACAATTTTTTT‑CTGCCAAAAACAACAAAAGGGAGTGGTTTATGTATATTTACTTTAATACACCAAGTGAAATTCCTT‑TGCCAt                                                                                                    >  2:565635/1‑238 (MQ=255)

                                                                                                                                           tGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGGAAGGCG‑CCATAAAAATTCAGGGAAAAAAATTATTTTTCG‑GTTTAATTTAC‑TAACCACATCATTTTTCTT‑CTGCTAATAAACACAAAGGTGAGTGGGTTTTGATTTTTTCCTTTAAAAAACCAGGTG‑AAATCCTTTTGCCAttc                                                                                                  >  2:1605542/1‑237 (MQ=255)

                                                                                                                                              gcacaaGACGTGTTTCGGCCTGCTGAAGGTTAAAACC‑TTCGGAAAAGTGGGGTTCCGCGTGGCGGTTGATGATAAAATTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATgcagg                                                                                               <  2:1874163/234‑1 (MQ=255)

                                                                                                                                                     aCGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TGCGGCAAAGGGGTGATCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATgcagg                                                                                               <  1:1389671/232‑1 (MQ=255)

                                                                                                                                                       ttttATCGGCCTGCTGAAGGGTAAAAAC‑TTCGACAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAATTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTctg                                                                                                                                                                              <  1:1815277/152‑1 (MQ=255)

                                                                                                                                                       gTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAg                                                                                                                                                      <  1:555106/176‑1 (MQ=255)

                                                                                                                                                       gTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGGGTAAGCCG‑CCATAAAAATCCAGGCAAAAAAATTATTTTTCG‑CTTTAATTAAA‑TAACCACAACATTGTTCTT‑CTGCTAATAAAAACAAAAGGGAGTGGGTTATGATTATTTACTTTTAAACAACAGGTG‑AAATTCTTTTGGCAAGTAGGCA‑GGGGTgg                                                                                     >  2:1536172/1‑239 (MQ=255)

                                                                                                                                                         ttATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATACAGGCAAAAAGGTTATTTTCAG‑CTTTAATTAAC‑TAACCAAATCATTGTTCTG‑CTGCTAATAACAAAAAAAGTGAGTGGTTTATGATTATTCACTTTTATACACCACGGG‑AATTCCTTCTGCCATGCATGCA‑GGGTTTGaa                                                                                   >  1:1916964/1‑238 (MQ=255)

                                                                                                                                                               gCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGCGGAAACCC‑CCCTAAAAAACCCGGCAAAAAACTTATTTTTAG‑CTTTTATTTAA‑TAAACCCATCATTTTTCTT‑CCTCTAATAACAACAAAAGTGAGTGGGTTTTGATTTTTTCCTTTTATAAACCCAGGG‑AATTCCTTCTGCCATTCAGGCA‑AGGTTGGa                                                                                    >  2:1389671/1‑232 (MQ=255)

                                                                                                                                                               gCCTGCGGAAGGGTAAAATC‑TTCGGCAAAGTGGTGACCCGCGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATg                                                                                                   <  1:18919/218‑1 (MQ=255)

                                                                                                                                                                    ctgAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAg                                                                                                                                                      <  1:1016057/163‑1 (MQ=255)

                                                                                                                                                                      gAAGGGAAAAAAC‑TTCGGCAAAGTGGTGAACCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCTTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGa                                                                      <  1:59148/239‑1 (MQ=255)

                                                                                                                                                                            tAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGGGTAAGCCC‑CCCTAAAAAACCAGGCAAAAAACTTATTTTCAG‑CTTTTATTAAC‑TAAACACAACATTGTTCTG‑CTGCTAATAACAACAAAAGTGAGTGGGTTATGATTATTTACTTTAATACAACAAGTG‑AATTTCTTCTGCCCTTCAGGGA‑GGGTTTGGCAGAAAAAACGCCAAGGaaa                                                                >  2:1090789/1‑238 (MQ=255)

                                                                                                                                                                            tAAAAAC‑TTCGGAAAAGTGGTCATCCGGGGGGGGGGTGAAGATTAAAGTTAAAACGGCGGCGAAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGaa                                                                 <  2:1404065/238‑1 (MQ=255)

                                                                                                                                                                                aaC‑TGCGGCAAAGGGGTGCTCCGGGTGGGGGGTAATAATAAAAGTTTCACCGGCGGCTAAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTaaa                                                            <  2:1709964/239‑1 (MQ=255)

                                                                                                                                                                                     tCGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAACGGCGGGGTAAGGCG‑CCCAAAAAATTCAGGGAAAAAAATTTTTTTTAG‑GTTTTATTAAA‑TAAACACATCATTGTTCTGCCTGTTATTAA‑AACAAAAGTGAGTGGGTTATGATTATTTACTTTTATTCAACAGGTG‑AAATACTTCTGTCATGGAGGCA‑GGGTTGGGAAGAAAAACAGCGAAGGAATTtaaaag                                                         >  2:249161/1‑236 (MQ=255)

                                                                                                                                                                                      cGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATtaac‑taa                                                                                                                                                                                              >  1:1259664/1‑107 (MQ=255)

                                                                                                                                                                                      cGGCAAAGGGGTGTTCCGCTTGGAGGATGATAATTAAATTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGcc                                                       <  1:1951477/239‑1 (MQ=255)

                                                                                                                                                                                       ggCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAAGTTTCAAAGGCGGCGTAAACCG‑CCATAAAAATCCAGGCAAAAAACTTATTTTTAG‑CTTTTATTAAC‑TAAACACAACATTTTTTTG‑CCGCTAATAAAAACAAAAGGGAGAGGGTTATGATTTTTTACTTTTATATACCCGGGG‑AATTCCTTTTGCCATTCAGGCAGGGGTTGGA‑AAAAAAAACCCGAAAAAATTtaaaatccc                                                      >  2:1159317/1‑234 (MQ=255)

                                                                                                                                                                                         cAAAGTGGTGACCCGCGGGGCGGGTGATGATTAAAGTTTCAACGGCGGCGGAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTctg                                                                                                                                                                              <  1:907761/120‑1 (MQ=255)

                                                                                                                                                                                              tggtgATCCGGGTGGCGGGTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTATC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGatta                                                                                                                                         <  1:782995/151‑1 (MQ=255)

                                                                                                                                                                                                      cgagTGGCGGGTGATAATTAAAGTTTCAACGGCGGCATAAACCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATg                                       <  1:2105462/236‑1 (MQ=255)

                                                                                                                                                                                                        cgTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGGAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAAACACATTATTGTTCTG‑CTGCTAATAAAAACAAAGGGGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTTTGGCATGCAGGCA‑GGGTTGGACAGAAAAAACGCGAAGGAATTAAAATGGCTGGGACGGGAAAAATGaa                                     >  1:1653477/1‑239 (MQ=255)

                                                                                                                                                                                                        cgTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGGAAGGCG‑CCATAAAAATCCAGGCAAAAAACTTATTTTTAG‑CTTTTATTAAC‑TAACCACAACATTTTTCTT‑CTGCTAATAAAAAAAAAAGGGAGTGGGTTTTGATTATTTACtttta                                                                                                                               >  2:782995/1‑149 (MQ=255)

                                                                                                                                                                                                               ggTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCCTAAAAATCCCGGCAAAAAGATTATTTTCAG‑CTTTTATTAAC‑TAACCACCTCATTTTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATTATTATTCCCTTTAATACACCAGGGG‑AATTCCTTCTGCCATGCAGGCA‑GGGGTGGACAGAAAACACCCGAAGGGATTAAAAATGCTGGAAAGGGGAAAAAGAAAAAAGTc                              >  2:2031580/1‑239 (MQ=255)

                                                                                                                                                                                                               ggTGATGATAAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGt                               <  1:69178/238‑1 (MQ=255)

                                                                                                                                                                                                                    agatTAAATTTACAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGttt                         <  1:1605542/238‑1 (MQ=255)

                                                                                                                                                                                                                          aaGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGa                                                   <  1:1804776/207‑1 (MQ=255)

                                                                                                                                                                                                                              ttCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGc                 <  1:1536172/237‑1 (MQ=255)

                                                                                                                                                                                                                                      cggcgTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTgaga        >  1:597693/1‑238 (MQ=255)

                                                                                                                                                                                                                                            aaGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGAcc  <  1:1771067/238‑1 (MQ=255)

                                                                                                                                                                                                                                             aGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGa                                                                      >  1:2042514/1‑169 (MQ=255)

                                                                                                                                                                                                                                             aGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGa                                                                      <  2:2042514/169‑1 (MQ=255)

AAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGG‑TTTAGA‑GAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAAC‑TTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCG‑CCATAAAAATCCAGGCAAAAAGCTTATTTTCAG‑CTTTAATTAAC‑TAACCACATCATTGTTCTG‑CTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTG‑AATTCCTTCTGCCATGCAGGCA‑GGGTTGGACAGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGGAAAAATGAAACATGTCAGTTTGACTCTGCAGGTTGAGAACGACC  >  NC_007779/1521563‑1522033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`