BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,315,451	A→C	15.4%	G211G (GGT→GGG)	ompC ←	porin OmpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,315,451	0	A	C	15.4%	286.4 / 6.2	102	G211G (GGT→GGG)	ompC	porin OmpC
Reads supporting (aligned to +/- strand): ref base A (62/20); new base C (2/16); total (64/38)
Fisher's exact test for biased strand distribution p-value = 4.99e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCA  >  NC_007779/2315214‑2315688

gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGaaa                                                                                                                                                                                                                                                 >  2:1212263/1‑238 (MQ=255)

    tctttcTGTTACTGAGCAACAGATTCGAATGTCTGGTCTTTGTTCGCCCAACCCAAGGAACCTACGCGAGTTGTGGT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCGCCAGTGTAGGTTTCAGCCCGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTATGAGCAGCAGTACTTTTGGAGCCGGCTGTTGCACCACCGATACCGAAGCC‑ttt                                                                                                                                                                                                                                           <  2:410647/234‑2 (MQ=255)

     cGAACTGGTATTGAGCACCAGCTTCGAAGTGCTGTGCTTTGTGCGCCCAACCCAGGGAACCTACGCGAGTTGCGTG‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGGGTCGTATTTCAGACAACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCCCCACCGATACCGAAACC‑TTCg                                                                                                                                                                                                                                          <  2:1697295/239‑1 (MQ=255)

     cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTc                                                                                                                                                                                                                                           <  1:40119/238‑1 (MQ=255)

     cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCg                                                                                                                                                                                                                                          <  1:31935/239‑1 (MQ=255)

     cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACACAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCATGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTc                                                                                                                                                                                                                                           <  2:642522/238‑1 (MQ=255)

       aaCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGATTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGAGTTCTGAGCATCAGTACGTCTGGAGCTGGAGATCGCACCACAGATACCGAACCC‑TTCGTa                                                                                                                                                                                                                                        >  2:1035092/1‑239 (MQ=255)

        aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTaa                                                                                                                                                                                                                                       <  1:415332/239‑1 (MQ=255)

             tACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAt                                                                                                                                                                                                                                   <  1:1156014/238‑1 (MQ=255)

              aCTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑a                                                                                                                                                                                                                                 <  2:1185804/238‑1 (MQ=255)

              aCTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑a                                                                                                                                                                                                                                 <  2:1851314/238‑1 (MQ=255)

               cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAg                                                                                                                                                                                                                               <  1:20238/239‑1 (MQ=255)

                  aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATTGCACCACCGATACCGAACCC‑TTCGTAATCAT‑AAGTGa                                                                                                                                                                                                                            >  2:124763/1‑239 (MQ=255)

                  aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGa                                                                                                                                                                                                                            >  1:292350/1‑239 (MQ=255)

                  aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGa                                                                                                                                                                                                                            >  1:504634/1‑239 (MQ=255)

                       cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGa                                                                                                                                                                                                                        >  1:1258723/1‑238 (MQ=255)

                       cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGa                                                                                                                                                                                                                        >  1:957604/1‑238 (MQ=255)

                            tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATCCCGAAACC‑TTCGTAATCAT‑AAGTGATAGAAccgcc                                                                                                                                                                                                                  <  1:1218961/239‑1 (MQ=255)

                            tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAAccgcc                                                                                                                                                                                                                  >  1:1685729/1‑239 (MQ=255)

                            tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATAGCACCACAGATACCGACACC‑TTCGTAATCAT‑AAGTGATAGAAccgca                                                                                                                                                                                                                  >  2:470392/1‑238 (MQ=255)

                                    cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGAcgccg                                                                                                                                                                                                           >  2:267162/1‑238 (MQ=255)

                                      tgttttttttCCCCCAACCCAGGAAACCT‑CGGGGATTGTGTTGGTGGGTTTGGGTGTCAGGGGCGGCCAGGTGGATGTTTTTGGGGTCGTTTTCAAGACCCCCGGTGTGGGTTTCAGCCCGGTCGCGGTTACC‑GATGTAAGCGGCGGTGTTCTGAGCATCAGTACGTTTGGAGCGGGAGATCGCCCCACCGATCCCGAACCT‑TTGGTAATCAT‑AAGTGATAGAACCGCCGAcgccgtcg                                                                                                                                                                                                        <  2:957604/235‑1 (MQ=255)

                                       tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGAcgccgtcg                                                                                                                                                                                                        >  1:620102/1‑238 (MQ=255)

                                                    cAACCCGGTGAACGTGCGAGATTTGGTTT‑GTGGTTCGGGGTGTCCCGAGCCGCGAGGTAGAGGTTGTTAGACCCATATTTCAGCCCCCCAGTGTATGTTCCACCATGGTCGCCGTTACC‑GATGTAGGCAGGGGTGTTCTGACCATCATTCCGTTGGGAGTGGGGCCTCCCCCCACCTATACCGAACCC‑TTGGTAACCAA‑AGGTAATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAg                                                                                                                                                                                          <  2:953854/239‑1 (MQ=255)

                                                    cAACCCGGGGACCCTACGGGTGTTGGGTT‑GTGGGTTTGGGTGTACTGAGCAGCAAGGTAGATGTGTTTGGCGTCGTATTTCAGACCCCCAGGGTGGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTGGGAGCGGGAGATCCCCCCCCCGATCCCGAACCT‑TTCGTAATCAT‑AAGTGATAGACCCGCCGACGCCGTCGCCGTTTTGACGCAg                                                                                                                                                                                          <  2:639282/239‑1 (MQ=255)

                                                      aCCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCGGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTg                                                                                                                                                                                        <  1:267162/239‑1 (MQ=255)

                                                               aCCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATCCCGAACCC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGAc                                                                                                                                                                               <  1:163881/239‑1 (MQ=255)

                                                                 cTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGACACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCATGTCGTCACGAcc                                                                                                                                                                              >  2:1005127/1‑238 (MQ=255)

                                                                 cTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                                                             >  1:1144112/1‑239 (MQ=255)

                                                                    cgcgAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgttg                                                                                                                                                                          >  1:491905/1‑239 (MQ=255)

                                                                                tggtgggTTTGGGTGTACGGGGCGGCAAGGTAGATGTTGTTGGCGTCGTATTTCAGCCCCCAGGTGTGGTTTTCAGCACGGCCGCCGTTACC‑GATGTAAGCGGCGGTTTTCTGAGCATCAGTACTTTGGGAGCGGGGGACCGCCCCACCGATACCGAACCC‑TTCGTAATCAT‑AAGTGAAAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGc                                                                                                                                                                <  2:271344/233‑1 (MQ=255)

                                                                                        cTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTaa                                                                                                                                                       >  2:33685/1‑239 (MQ=255)

                                                                                             tgtACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑a                                                                                                                                                                                                                                 >  1:1665880/1‑160 (MQ=255)

                                                                                             tgtACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑a                                                                                                                                                                                                                                 <  2:1665880/160‑1 (MQ=255)

                                                                                             tgtACTGAGCAGCCAGGGAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCt                                                                                                                                                  >  1:395052/1‑239 (MQ=255)

                                                                                                   gAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCa                                                                                                                                            >  1:1638490/1‑239 (MQ=255)

                                                                                                    agcagcCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAg                                                                                                                                           >  1:998347/1‑239 (MQ=255)

                                                                                                              tAGATGTTGTTGGCGTGTTTTTCCAGCCCCCCGGTGTGGGTTCAAGCAGGGTCGCCGTTACC‑GATGTAAGCAGCGGGTTTCGGAACATAAGTACGTTGGGAGCGGGGGACCCCCCCCCCGATCCCGAACCC‑TTCGTAATAAG‑AAGGGAAAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGc                                                                                                                                  <  2:1258723/238‑1 (MQ=255)

                                                                                                                     tgttAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGttttt                                                                                                                           >  1:475499/1‑238 (MQ=255)

                                                                                                                     tgttAGCGCCGTATTTCAGACCCCCTGGGTAGTTTACAGCCCGGTCG‑CGTTACCGGATGTAGGCAGGGGTGTTCTGAGAAACATTAGGTTGGGAGGTGGAGACCCCCCCACCGATACCGAACCC‑TTCGAAACAAT‑AAGTAAAAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa                                                                                                                          <  2:504634/239‑1 (MQ=255)

                                                                                                                      gttAGCGTCGTATTTCAGACCACCAGTGTAGGTCTTAGCACTGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa                                                                                                                          >  2:314011/1‑238 (MQ=255)

                                                                                                                       ttAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAc                                                                                                                         >  1:1620226/1‑238 (MQ=255)

                                                                                                                        tAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTCCGTTTGGAGCTGGAGATCGCGCCCCCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                                                       <  1:1212263/239‑1 (MQ=255)

                                                                                                                           cgtcgtATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTgg                                                                                                                    >  1:1312751/1‑239 (MQ=255)

                                                                                                                           cgtcgtATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGGAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTgg                                                                                                                    >  2:1192357/1‑239 (MQ=255)

                                                                                                                              cgaaTTACAGCCCGCCAGTGAAGGTTTCAGCGCGTTGGCCGTTCCC‑GAGGTAAGCAGCGGTGTTCGGAACATCAGTACGTTGGGAGCTGGAGACCGCCCCACCGATCCCGAACCT‑TTGGAAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTa                                                                                                                  <  2:491905/235‑1 (MQ=255)

                                                                                                                                  tttCAGCCCACCGGTGTTGTTTCCAGCACGTCGGCCGTTACC‑GATGTAAGCAGCGGTTTTTGGGGCATCAGTCTGTTGGGAGGGGGAGATCCCCCCCCCGATACCGAACCT‑TTCGTAATCAT‑AGGTGAAAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa                                                                                                             <  2:1144112/239‑1 (MQ=255)

                                                                                                                                  tgtgagcccaccgGGGTAGTTTCCAGCACGGTCGCCGTTACC‑GGTGTAGGAAGCGGTTTTGGGAGCATCAGTGCTTTGGGGGAGGGAGCCCGCCCCACCGATACCGAATCT‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa                                                                                                             <  2:292350/232‑1 (MQ=255)

                                                                                                                                          caccaGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                                                                     >  1:307641/1‑239 (MQ=255)

                                                                                                                                          caccaGTGTAGATTTCAGCACGGGCGGCGTTATT‑GATGAAAGAAACGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGATATCGCACCACCGATACAGAAACC‑TTCGTAATCCT‑AAGTGATAGAACCGTCGTCGCAGTCCCGTTTTTGAGTCATGGCGTCACGACCCTTGTTGGTTAGCCCACCAGTACAGCCTCCACCACACAGGGTCACGTTTTTAACCTGGTGCGGCACACCAAAg                                                                                                     >  2:1534940/1‑239 (MQ=255)

                                                                                                                                            ccaGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt                                                                                                    >  1:1114792/1‑238 (MQ=255)

                                                                                                                                                tgtggttttCAGCCCGGGCGCCGTTCCC‑GAGGAAAGCGGCGGTGTTCGGAGCATCAGTACGTTGGGAGCTGGAGATCGCACCCCCGATACCGAACCC‑TTCGTAATCAT‑AAGTAATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGc                                                                                                                                                                <  2:1204290/168‑1 (MQ=255)

                                                                                                                                                  tgggTTTCAGCCGGGCCGCCTTTACG‑GATGTAGGCGGGGTTTTTCTGAGCATCAGTCCTTTGGGAGCTGGAGATCCCCCCACCGATACCGAACCC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc                                                                                             <  1:619413/237‑1 (MQ=255)

                                                                                                                                                   gggTTTCAGCCGGGTGGCCTTTACC‑GATGTAAGCAGCGGTTTTCTGAGCCGCAGTACGTTTGGAGGTGGAGCCCCCCCCACCGATCCCGAACCC‑TTCATAATCAA‑AGGTGAAAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGcc                                                                                             <  2:112821/237‑1 (MQ=255)

                                                                                                                                                     gTTTCAGCACGGTCGCCGTTACT‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc                                                                                          >  2:1340792/1‑239 (MQ=255)

                                                                                                                                                     gTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc                                                                                          >  1:315993/1‑239 (MQ=255)

                                                                                                                                                       ttCGGCAGGGTCCGCGTCATC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa                                                                                        >  2:652821/1‑239 (MQ=255)

                                                                                                                                                          ggcggggTCGGCGTTACT‑GAAAAAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGAACGTTGTTAGTTACGCGACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAGAGTTCAGGCCGACacaca                                                                                     >  2:1241084/6‑235 (MQ=255)

                                                                                                                                                          aGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTaaa                                                                                                                                                      >  1:1204290/1‑174 (MQ=255)

                                                                                                                                                          aGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     >  1:1547738/1‑239 (MQ=255)

                                                                                                                                                          aGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     >  2:1135080/1‑239 (MQ=255)

                                                                                                                                                          aGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     >  1:794056/1‑239 (MQ=255)

                                                                                                                                                          aGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     >  1:1710436/1‑239 (MQ=255)

                                                                                                                                                               ggTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg                                                                                >  2:158296/1‑239 (MQ=255)

                                                                                                                                                                 tCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                                              >  2:1014710/1‑239 (MQ=255)

                                                                                                                                                                        tACC‑GATGTAAGCAGCGGTGTTCTGAGCACCAGTACTTTTGGAGCTGGAGACCGCCCCACCGATACCGAAACC‑TTCGAAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa                                                                       <  1:333601/239‑1 (MQ=255)

                                                                                                                                                                                gTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCC‑CTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACg                                                               >  1:420540/1‑239 (MQ=255)

                                                                                                                                                                                        cGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTc                                                        >  2:1049679/1‑239 (MQ=255)

                                                                                                                                                                                         ggTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTc                                                        >  1:1814865/1‑238 (MQ=255)

                                                                                                                                                                                           tgtTCTGAGCATCAGTACGTTTGGCGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGAGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCTGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCTGAGGGGCTGACGTTTTTACCCTGGTACTGAACAGCAACGTTCAGGCCGTCAACAAGCCCGAAGAAGTCAGTGTTACGGTAGGTcggg                                                     >  2:1311364/1‑237 (MQ=255)

                                                                                                                                                                                            tttCTGGGCATCAGTGCTTTGGGGTCGGGAGACCGCCCCACCGATACCGAACCC‑TTCGTAAACAT‑AAGGGATGGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGa                                                    <  2:1638490/238‑1 (MQ=255)

                                                                                                                                                                                                  aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     >  2:1270096/1‑200 (MQ=255)

                                                                                                                                                                                                  aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                     <  1:1270096/200‑1 (MQ=255)

                                                                                                                                                                                                  aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                              >  1:429713/1‑239 (MQ=255)

                                                                                                                                                                                                  aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                              >  1:1808451/1‑239 (MQ=255)

                                                                                                                                                                                                   gCATCGTTACGTTTGGGGCGGGAGATCCCCCCACCGTTACCGAACCT‑TTCGTAATAAA‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                              <  2:475499/238‑1 (MQ=255)

                                                                                                                                                                                                     aTCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa                                            >  1:648250/1‑238 (MQ=255)

                                                                                                                                                                                                     aTCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa                                            >  2:750417/1‑238 (MQ=255)

                                                                                                                                                                                                       cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAcc                                          >  1:684281/1‑238 (MQ=255)

                                                                                                                                                                                                       cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAcc                                          >  1:1388043/1‑238 (MQ=255)

                                                                                                                                                                                                       cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAcc                                          >  2:113315/1‑238 (MQ=255)

                                                                                                                                                                                                           ccGTTTGGGGTTGGAGACCGCCCCCCCAATACCGACCCA‑TTCGTAACCAT‑AAGTGACAGAGCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGCCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgct                                     <  2:1696192/238‑1 (MQ=255)

                                                                                                                                                                                                               ttGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct                                  >  1:242397/1‑238 (MQ=255)

                                                                                                                                                                                                                    gcgggAGATCCCCCCCCCGATA‑CGAAACCTTTCATAATCAT‑AGGTAAAAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg                             <  1:1005127/235‑1 (MQ=255)

                                                                                                                                                                                                                    gCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc                                                      <  1:99728/213‑1 (MQ=255)

                                                                                                                                                                                                                    gCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa                            >  1:533441/1‑239 (MQ=255)

                                                                                                                                                                                                                    gCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa                            >  1:1751761/1‑239 (MQ=255)

                                                                                                                                                                                                                      tGGAGATCGCACCCCCGATCCCGAACCC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGaa                           <  1:119784/238‑1 (MQ=255)

                                                                                                                                                                                                                        gagaTCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAgg                                                          >  1:932151/1‑205 (MQ=255)

                                                                                                                                                                                                                        gagaTCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGtt                        >  1:1044294/1‑239 (MQ=255)

                                                                                                                                                                                                                         agaTCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg                       >  1:1222393/1‑239 (MQ=255)

                                                                                                                                                                                                                                  caccGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg              >  1:612960/1‑239 (MQ=255)

                                                                                                                                                                                                                                  aaccGATACGGAAACT‑TGCTTAA‑CATAAAGGGAAAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg              <  2:1501322/238‑1 (MQ=255)

                                                                                                                                                                                                                                   accGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGt             >  2:1516045/1‑239 (MQ=255)

                                                                                                                                                                                                                                       aTACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt         >  1:1372635/1‑239 (MQ=255)

                                                                                                                                                                                                                                       aTACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt         >  1:1294489/1‑239 (MQ=255)

                                                                                                                                                                                                                                          ccGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTc      >  1:577724/1‑239 (MQ=255)

                                                                                                                                                                                                                                            gAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca     >  1:518456/1‑238 (MQ=255)

                                                                                                                                                                                                                                             aaaCC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcac    >  2:266767/1‑238 (MQ=255)

                                                                                                                                                                                                                                               aCC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcacca  >  1:1811051/1‑238 (MQ=255)

GAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTT‑GTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACC‑GATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACC‑TTCGTAATCAT‑AAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCA  >  NC_007779/2315214‑2315688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`