BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	632,320	T→C	25.5%	T127T (ACA→ACG)	hcxA ←	hydroxycarboxylate dehydrogenase HcxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	632,320	0	T	C	25.5%	323.6 / 120.5	184	T127T (ACA→ACG)	hcxA	hydroxycarboxylate dehydrogenase HcxA
Reads supporting (aligned to +/- strand): ref base T (80/56); new base C (29/18); total (109/75)
Fisher's exact test for biased strand distribution p-value = 8.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

Only 100 of 188 total aligned reads displayed.

CGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCC  >  NC_007779/632082‑632557

cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGgt                                                                                                                                                                                                                                                >  1:611186/1‑239 (MQ=255)

        gCTTGCGCTTTATTGACCCCCATGCGCGCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCCCCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAgg                                                                                                                                                                                                                                         <  2:1576515/238‑1 (MQ=255)

            gcgcATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAACTCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAggcggc                                                                                                                                                                                                                                     >  1:1533967/1‑238 (MQ=255)

            gcgcATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAggcggc                                                                                                                                                                                                                                     >  1:74298/1‑238 (MQ=255)

                tttattGACCCCCAGCCGCGCGGTTAGCGCAAACTTTTCTGGTTGCGGGGCCACCCCCACCGTTTAATCCCATTCCGCCAGC‑GTGTCACCGACCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAg                                                                                                                                                                                                                                 <  2:611186/237‑1 (MQ=255)

                            cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGAtcg                                                                                                                                                                                                                    >  1:160594/1‑239 (MQ=255)

                              gTCGCTCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GGGTCACCGATCCCCGCCAGCAGATATTGATGCGGTGCATTGGGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGAtcgtc                                                                                                                                                                                                                  >  2:1155955/1‑239 (MQ=255)

                              gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGAtcgtc                                                                                                                                                                                                                  >  1:1009344/1‑239 (MQ=255)

                              gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGAtcgtc                                                                                                                                                                                                                  >  1:1363836/1‑239 (MQ=255)

                                     ggTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACgg                                                                                                                                                                                                           <  1:1334418/239‑1 (MQ=255)

                                     ggTTAGCGCCAACGTTTCGGGTGCCGGAGCCAGCACCCCCGCTTCATACCTTTTCGCCAGC‑TTGTCACCGCTCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACgg                                                                                                                                                                                                           <  1:518447/239‑1 (MQ=255)

                                       ttAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGca                                                                                                                                                                                                         >  1:977617/1‑239 (MQ=255)

                                           cGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGcaaca                                                                                                                                                                                                      <  1:396568/238‑1 (MQ=255)

                                                 cGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCa                                                                                                                                                                                               >  1:175301/1‑239 (MQ=255)

                                                 cGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCa                                                                                                                                                                                               >  2:79224/1‑239 (MQ=255)

                                                 cGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAAAAAACGGCa                                                                                                                                                                                               >  2:840328/1‑239 (MQ=255)

                                                  gTTTCTGGTGGCGGGGCCACCACCACCGTTTCAAAACATTTGGACGGT‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCACCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGTCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCa                                                                                                                                                                                               <  2:1533967/238‑1 (MQ=255)

                                                           tGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGAcg                                                                                                                                                                                     >  2:1592265/1‑239 (MQ=255)

                                                           tGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGAcg                                                                                                                                                                                     >  1:1366509/1‑239 (MQ=255)

                                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGAcggc                                                                                                                                                                                   >  1:1555696/1‑239 (MQ=255)

                                                                  ccaccccccccGCTTAATCCCATTTCGCCAGG‑GGGTCCCCGACCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACggcgggc                                                                                                                                                                               <  2:1015169/229‑1 (MQ=255)

                                                                   cAGCACCCCCGCTTCATACCATTTCGCCAGC‑GTGTCCCCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACggcgggcg                                                                                                                                                                              <  1:1501426/238‑1 (MQ=255)

                                                                       acccccTCTTCTTCCCATTTCG‑CAGCGGTGCCCCCGACCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGc                                                                                                                                                                         <  2:1370650/239‑1 (MQ=255)

                                                                       accaccGCTTCATCCCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAg                                                                                                                                                                            <  1:258791/236‑1 (MQ=255)

                                                                       accaccGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAg                                                                                                                                                                            <  1:778616/236‑1 (MQ=255)

                                                                          accGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCtt                                                                                                                                                                       >  2:1366949/1‑238 (MQ=255)

                                                                            cGCTTCATCCCCTTCCGCCCGC‑GTGTCCCCGTTCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcg                                                                                                                                                                    <  2:160594/239‑1 (MQ=255)

                                                                            cGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcg                                                                                                                                                                    <  1:1366949/239‑1 (MQ=255)

                                                                              cTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGCCGGCGGGGGGCTTTcgcg                                                                                                                                                                  >  2:237242/1‑239 (MQ=255)

                                                                               ttCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                                                                                                                  <  1:87242/238‑1 (MQ=255)

                                                                               ttCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                                                                                                                  <  1:1390450/238‑1 (MQ=255)

                                                                                tCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGGCAGGGCTTTCGCggg                                                                                                                                                                >  2:1783440/1‑238 (MQ=255)

                                                                                   tACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCCGGCGAGGGCCTTCGCGgggt                                                                                                                                                              >  2:367280/1‑238 (MQ=255)

                                                                                      cATTTCCCCAGC‑GTGTCCCCGACCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAg                                                                                                                                                          <  1:1713970/239‑1 (MQ=255)

                                                                                        tttCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCa                                                                                                                                                        >  1:1484365/1‑239 (MQ=255)

                                                                                             ccAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGc                                                                                                                                                    >  1:1133491/1‑238 (MQ=255)

                                                                                                      tCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCAcc                                                                                                                                           >  1:1594948/1‑239 (MQ=255)

                                                                                                              ccccGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCaa                                                                                                                                   <  1:1696555/239‑1 (MQ=255)

                                                                                                                ccGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATc                                                                                                                                 >  1:933582/1‑239 (MQ=255)

                                                                                                                           tatTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTg                                                                                                                        <  1:1570344/237‑1 (MQ=255)

                                                                                                                             ttgttgCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgt                                                                                                                    <  2:464389/239‑1 (MQ=255)

                                                                                                                             ttgttgCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgt                                                                                                                    <  1:1661912/239‑1 (MQ=255)

                                                                                                                                  gCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgtcgtc                                                                                                                >  1:1704279/1‑238 (MQ=255)

                                                                                                                                  gCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCa                                                                                                               <  1:250587/239‑1 (MQ=255)

                                                                                                                                  gCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCa                                                                                                               <  1:601507/239‑1 (MQ=255)

                                                                                                                                     gTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGa                                                                                                                                                           <  1:1048665/192‑1 (MQ=255)

                                                                                                                                     gTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGa                                                                                                                                                           >  2:1048665/1‑192 (MQ=255)

                                                                                                                                        cATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCgg                                                                                                           >  1:1239091/1‑237 (MQ=255)

                                                                                                                                               gATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAgcc                                                                                                   <  2:1663634/238‑1 (MQ=255)

                                                                                                                                                aTAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAgcc                                                                                                   <  1:991229/237‑1 (MQ=255)

                                                                                                                                                  aaTCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCa                                                                                               >  1:203933/1‑239 (MQ=255)

                                                                                                                                                  aaTCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCa                                                                                               >  1:1358132/1‑239 (MQ=255)

                                                                                                                                                  aaTCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAACAGGGCACCGCCCCCGACGCCAATCACCACGCTGCGGGCGTCACCGGACCCAgccccc                                                                                                >  2:1171548/1‑238 (MQ=255)

                                                                                                                                                    tctcCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCggg                                                                                                                                                          >  1:666307/1‑176 (MQ=255)

                                                                                                                                                    tctcCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAgt                                                                                             >  1:623718/1‑239 (MQ=255)

                                                                                                                                                       ccGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGCCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAgttgt                                                                                          >  1:756642/1‑239 (MQ=255)

                                                                                                                                                       ccGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGTGCACCGCCACCGACGCCAATCACCCCGCTCCGGTCGTCACCGGACCCAGCCCCCCgttgt                                                                                          >  2:396490/1‑239 (MQ=255)

                                                                                                                                                              caccaGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑t                                                                                  <  2:1704279/239‑1 (MQ=255)

                                                                                                                                                              caccaGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACa                                                                                    >  1:811024/1‑238 (MQ=255)

                                                                                                                                                              caccaGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACa                                                                                    >  1:1589206/1‑238 (MQ=255)

                                                                                                                                                                        aTAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTc                                                                           >  1:1811918/1‑236 (MQ=255)

                                                                                                                                                                         tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGGGCCCCGCCCCCGACGCCAATCACCCCCCTGCGGGCGTCCCCGGCACCAGCCGCCCGTTTGTGTACA‑ACGCTTTCgtc                                                                        >  2:673388/1‑236 (MQ=255)

                                                                                                                                                                         tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgt                                                                                                                    >  1:82153/1‑195 (MQ=255)

                                                                                                                                                                         tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCt                                                                        >  1:1646454/1‑238 (MQ=255)

                                                                                                                                                                                 gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAgg                                                                                                                                                                                                                                         >  1:1471669/1‑70 (MQ=255)

                                                                                                                                                                                 gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACg                                                                                                                                       <  1:383786/168‑1 (MQ=255)

                                                                                                                                                                                 gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCCGTTGTTGTACA‑TCGCTTTCGCTGCAATGAc                                                                >  2:566917/1‑238 (MQ=255)

                                                                                                                                                                                 gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGAcc                                                               >  1:446739/1‑239 (MQ=255)

                                                                                                                                                                                 gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGATGTCCAgg                                                                                                                                                                                                                                         <  2:1471669/70‑1 (MQ=255)

                                                                                                                                                                                                 cATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAatat                                                 >  1:378573/1‑237 (MQ=255)

                                                                                                                                                                                                  aTAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCt                                              <  2:203933/239‑1 (MQ=255)

                                                                                                                                                                                                  aTAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCt                                              <  2:719246/239‑1 (MQ=255)

                                                                                                                                                                                                      tGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGc                                          <  1:101415/239‑1 (MQ=255)

                                                                                                                                                                                                        cAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGCGACGGCGGGCGGGGGCCTTCGCGGGTTCTAGCACTGCCCCCCCACCGACGCCACTCACCCCCCCGCGGGCGTCACCCGGCTCACCCGCCCGTTGTTGTACATTCCCTTCCCCGCCAATCA‑CGGGAAACAAAACATGGTTTgtc                                         >  2:1001715/1‑236 (MQ=255)

                                                                                                                                                                                                         aGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGccc                                        >  2:86959/1‑238 (MQ=255)

                                                                                                                                                                                                           cgcCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTTCAATGACCGCGAAACAAAATATGCTTTGCCCCTg                                     >  1:21296/1‑239 (MQ=255)

                                                                                                                                                                                                           cgcCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTg                                     >  2:1495194/1‑239 (MQ=255)

                                                                                                                                                                                                            gcCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTgg                                    <  2:933582/239‑1 (MQ=255)

                                                                                                                                                                                                            gcCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTgg                                    <  1:1313163/239‑1 (MQ=255)

                                                                                                                                                                                                            gcCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTgg                                    <  2:623718/239‑1 (MQ=255)

                                                                                                                                                                                                            gcCTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTgg                                    <  1:716433/239‑1 (MQ=255)

                                                                                                                                                                                                             ccTGTCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTTCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGc                                   <  2:21296/239‑1 (MQ=255)

                                                                                                                                                                                                                gTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGCCGCCAATCACCACGCCGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAATAATACTTTGCCCCTgcccg                                 >  2:1414924/1‑234 (MQ=255)

                                                                                                                                                                                                                 tCCGGCATCATTATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGt                                >  1:683080/1‑238 (MQ=255)

                                                                                                                                                                                                                     gCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCaa                            >  1:723633/1‑238 (MQ=255)

                                                                                                                                                                                                                     gCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCaa                            >  1:297072/1‑238 (MQ=255)

                                                                                                                                                                                                                           ttATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCCCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGccg                      >  2:626359/1‑236 (MQ=255)

                                                                                                                                                                                                                           ttATACCAGACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTg                      >  1:428772/1‑238 (MQ=255)

                                                                                                                                                                                                                             ataCCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCg                   <  2:1358132/239‑1 (MQ=255)

                                                                                                                                                                                                                             ataCCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCg                   <  1:1291762/239‑1 (MQ=255)

                                                                                                                                                                                                                                   gACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCCGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCCGGCAGGCCAAACGCcggcgggaatt             >  2:650095/1‑234 (MQ=255)

                                                                                                                                                                                                                                   gACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAgtt             >  2:162156/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACg                                                                                                                                                                                                  <  2:1868139/59‑1 (MQ=255)

                                                                                                                                                                                                                                   gACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACg                                                                                                                                                                                                  >  1:1868139/1‑59 (MQ=255)

                                                                                                                                                                                                                                   gACGGAGAGCGGCGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAgtt             >  1:366258/1‑239 (MQ=255)

                                                                                                                                                                                                                                      ggAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAgtttgg          >  1:1729977/1‑239 (MQ=255)

                                                                                                                                                                                                                                        agagCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCCATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCCCCTTCGCTGCAATGACCGCGAAACAAAATAAGCTTTCCCCCTGGCAGTCCAAAAGCACGAGGAAgattggct        >  2:486482/1‑237 (MQ=255)

                                                                                                                                                                                                                                        agagCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATcacca                                                                                                                             <  2:1563494/123‑1 (MQ=255)

                                                                                                                                                                                                                                              gtgtCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGcgcc  >  1:794054/1‑239 (MQ=255)

                                                                                                                                                                                                                                              gtgtCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGTGCACCGCCACCGACGCCCATCACCCCGCCGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCCTTGCCCCTGGCAGTCCAAACGCTGGCGGGAGTTTGGTTTtcgc   >  2:279449/1‑238 (MQ=255)

CGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGC‑GTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACA‑TCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCC  >  NC_007779/632082‑632557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`