BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	954,796	C→G	9.6%	P31P (CCG→CCC)	focA ←	formate transporter FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	954,796	0	C	G	9.6%	262.5 / 2.6	118	P31P (CCG→CCC)	focA	formate transporter FocA
Reads supporting (aligned to +/- strand): ref base C (60/41); new base G (3/9); total (67/51)
Fisher's exact test for biased strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 119 total aligned reads displayed.

CAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954559‑955017

cAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGc                                                                                                                                                                                                                                  <  1:602884/238‑1 (MQ=255)

         ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       <  2:1080985/239‑1 (MQ=255)

         ccAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       <  1:871723/239‑1 (MQ=255)

         ccAGGCGATGGGCCCACTCGCCTTAGCAACAACAATGAGCACGGTGGAGGGAGAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAAGCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTGGCTGTGATATAGAAGACGAATGCGATTGGGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       <  2:554486/239‑1 (MQ=255)

                  gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCCGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTtat                                                                                                                                                                                                              >  1:162939/1‑239 (MQ=255)

                  gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGt                                                                                                                                                                                                                      <  2:1213545/231‑1 (MQ=255)

                  gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGt                                                                                                                                                                                                                      >  1:1213545/1‑231 (MQ=255)

                  gcgcCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAAACGCAAGATAGAAAAACCTTAGAG‑GATGTTTCCGTCCTTtat                                                                                                                                                                                                              >  2:1353042/1‑239 (MQ=255)

                   cgcCCACACGCCTTAGCAACAACAAGCAACACGGTGGAAGGATAGAGATCGGGTCCGCAGGCAACACCAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACGAGTTTTGTAAGGCCGAAGGGCATTGTGCATGGGCCAGTGGTTGCGGTGATATAGAAGACGAATGCGATTGAGATGAAAACGCC‑GTCGGTAATCGCCAAGTAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTtat                                                                                                                                                                                                              <  2:404151/238‑1 (MQ=255)

                                tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCctct                                                                                                                                                                                               >  1:1254903/1‑239 (MQ=255)

                                      caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACAACCGCCTCTTCGGc                                                                                                                                                                                          >  1:2062649/1‑238 (MQ=255)

                                       aacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGTCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTtat                                                                                                                                                                                                              >  1:694930/1‑218 (MQ=255)

                                       aacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGTCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTtat                                                                                                                                                                                                              <  2:694930/218‑1 (MQ=255)

                                        acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAAATAAAAAAGCTTGAGCG‑GGAGTTTAGGTGCTTTAT‑AAACACCAGCCCCTTCGGCCa                                                                                                                                                                                        >  2:1034940/1‑238 (MQ=255)

                                                 acGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       <  1:185790/199‑1 (MQ=255)

                                                    gTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGGAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGCAAGTATTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACCTTGGc                                                                                                                                                                                 >  1:978548/1‑233 (MQ=255)

                                                    gTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTg‑c                                                                                                                                                                          >  1:1702743/1‑239 (MQ=255)

                                                        aaGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGt                                                                                                                                                                                                                      <  1:1397886/193‑1 (MQ=255)

                                                          gaaaagatacaggCCCCGAAAACAACACAAGCAATCAGCCCCGGAAAGAAACAAATCCCGCCAACAATTTTTACCGGGCCGAAGGGCTTTGTGCCGGGGCCTGTGGTTGCTTTGAAATAAAAGAAAAATGCGGTTGAGAAGAAACCCCG‑GGCGTTAATCGCCAAATAGAATTTCTT‑AGCGTGATTTTTGTTTGTTTTAA‑GAACACCCGCCTCTTCGGCCACTTTGGCCATTg‑cagca                                                                                                                                                                      <  2:1067265/226‑1 (MQ=255)

                                                          gTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAgg                                                                                                                                                                    >  1:2032137/1‑239 (MQ=255)

                                                             aaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAg                                                                                                                                                                 >  1:473823/1‑239 (MQ=255)

                                                             aaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAg                                                                                                                                                                 >  1:194509/1‑239 (MQ=255)

                                                             aaGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAAACGCCACATAGAAAGTCTTTAGCG‑GAGGGTTCGGTGCTTTAT‑AAACAACCCCCTCTTTCGCCACCTTTGCCCTTG‑ACGCAGGAAg                                                                                                                                                                 >  2:849735/1‑239 (MQ=255)

                                                               gagaTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑cagcagctggc                                                                                                                                                                >  1:897754/1‑233 (MQ=255)

                                                                 gaTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGGGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACCCC‑GGCGGTAATCGCCAGATAGAAAGTCTAAAGCG‑GATGTTTCGTTGCTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTaa                                                                                                                                                              <  1:1179552/238‑1 (MQ=255)

                                                                  aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTTGCCATTG‑CAGCAGGAAGTaaaa                                                                                                                                                            >  1:1074716/1‑239 (MQ=255)

                                                                  aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACAACCGCCTCTTCGGCCCCTTTGGCCATTG‑CCGCAGGAAGTaaaa                                                                                                                                                            >  1:1834858/1‑239 (MQ=255)

                                                                  aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAAGCTTAAGCG‑GATGGTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCCTTG‑CAGCAGTAAGTaaaa                                                                                                                                                            >  1:1088233/1‑239 (MQ=255)

                                                                     ggCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       >  1:988005/1‑179 (MQ=255)

                                                                     ggCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCtt                                                                                                                                                                                              >  1:1265218/1‑203 (MQ=255)

                                                                     gcccccGCAAACAACACAAAGAATCAGCCCCAAAGAGAGGCAAACGCCGACAACCGTTATTGCCAGGCGGAAGGGAGTGGTGCGTGTCCCAGTGTTTGCTATAAAAAAGAAGACGAATGAGTTTGAGATAAAACCCCC‑GGGGATAATCGCAAGAAAGAAAGTTTTAAGGG‑GTTGTTTTGTTGTTTTAA‑AAACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAg                                                                                                                                                           <  2:1053636/233‑1 (MQ=255)

                                                                               acaacaCAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACCCC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGTTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGAt                                                                                                                                                         <  1:345154/229‑1 (MQ=255)

                                                                                      aaaGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCt                                                                                                                                        >  1:142899/1‑239 (MQ=255)

                                                                                             cAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTTGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGGCAGCTTTtac                                                                                                                                   >  1:2102316/1‑237 (MQ=255)

                                                                                             cAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAAACGCCCCATAGAAAGATTTTAGGG‑GATGGTTTTTTGCTTTAT‑ATACCCACCCCCCCTCTGCCACTTTGGCCATTG‑CAGCAGGTAGGAAAAAATCACAAGGCTTGTACACTTTCacac                                                                                                                                 >  2:1744397/1‑239 (MQ=255)

                                                                                              aGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACAAC‑GGCGGGAATCTCCAGATAGAAAGTAATAAGAG‑GATGTCTCTTTGCTTTTA‑TGAACCAAGCCTCTTTGGCCACTTTGACCATTA‑CAGCAGCAAATAAAAGATCAAAATGGTTGTCAgtattgtacct                                                                                                                                >  2:1864868/1‑229 (MQ=255)

                                                                                              aGCCCCAGAGAGAAGCAAACGCCGCCAACCAGTTTTGCCATGCCCAAGGGCACTGTGCCTGTGCCAGCGGTTGCTGTGATATAGCAGACGAATGCGATTGAGATGAAAACACC‑GGCGGCAACAGTAACGTAGAAAGTCATAACAG‑GCTGAGTATTTGCGTTCT‑AAATCCCTGCATCCTCAGCAAGCTTGAGCATTT‑CAGGAGCAAGAAAAAAGTCAGAAAGGTTTTCAGCTTTCaaacc                                                                                                                                >  2:376535/1‑238 (MQ=255)

                                                                                                ccccAGAGAGATGCCAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCCGTGGTTGCTGTGATCTAGAAGACGAATGAGATTGAGATGAAAACACC‑GGCGGCAACCGCCAGAAAGAAAAACTTAAGCG‑GACGATTCGTTACTTTAT‑AGACCCCCCCGCTTTCGCCCACCTCCGCCATTT‑ACGCAGAGAAAATAAAACCAAAAGGGCCGTGAGCTTTccacacaa                                                                                                                              >  2:257246/1‑232 (MQ=255)

                                                                                                ccccAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTTGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACCCTa                                                                                                                               >  1:1168587/1‑238 (MQ=255)

                                                                                                ccccAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTa                                                                                                                               >  1:939513/1‑238 (MQ=255)

                                                                                                    agagagAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGGTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActc                                                                                                                           >  1:2022685/1‑238 (MQ=255)

                                                                                                               aaTGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa                                                                                                               >  1:1065390/1‑239 (MQ=255)

                                                                                                                aTGCCGCCATCCTGTTTTGCCATGAGGAAGGGTATTGTGGCTGTGCAGGTGGTTGCTGTAATAAAGAAGACGAATGCGATGGAGATAAAACCCCC‑GGCGGTAACCGCCAGATAAAATGTTTAAAGGG‑GTTTTTTGGTTTCTTTAA‑AGCCCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAg                                                                                                              <  2:473823/239‑1 (MQ=255)

                                                                                                                aTGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGc                                                                                                                                                                                 >  1:1737145/1‑173 (MQ=255)

                                                                                                                                   ccAGGCGGAAGGGAATGGTGCCTGGGCAAGGGTTTGCTGTGATATAGAAGACAAATGCGATGGAGATAAAACCACC‑GGCGGTATTCCCCATATGGAAAGTTATATGTG‑TATGTTGCGTTCCTTATA‑AAACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAAc                                                                                           <  1:1394620/239‑1 (MQ=255)

                                                                                                                                        ccGAAGGGCTTTGTGCCTGTGCCGGTGGTTGCTTTGATATAGAAGACGAATGCGATTAAGAGAAAAACCCC‑GGCGTTAACCGCCAGATAGAAAGTCTTAAGCG‑GTTGTTTCTTTGTTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAct                                                                                                                            <  1:1198194/201‑1 (MQ=255)

                                                                                                                                                cATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       <  1:2112734/104‑1 (MQ=255)

                                                                                                                                                  ttGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGGG‑Ga                                                                                                                                                                                                                              >  2:1238668/1‑95 (MQ=255)

                                                                                                                                                  ttGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑Ga                                                                                                                                                                                                                              <  1:1238668/95‑1 (MQ=255)

                                                                                                                                                   tgtgcctgtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGTTTGAGATAAAACCCCC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GTTGTTTCTTTGTTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           <  1:44042/239‑1 (MQ=255)

                                                                                                                                                     tgcctgtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCCGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATgaga                                                                         >  1:1236503/1‑239 (MQ=255)

                                                                                                                                                     tgcctgtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATgaga                                                                         >  1:296262/1‑239 (MQ=255)

                                                                                                                                                       cgtgggccGGTGGTTGCTGTGATATAAAAGAAGAATGATATGGAAAGAAAAACCCC‑GGGGGTAACCGCCAGATAGAAAGTCTAAAGCG‑GTTGTTTCGTTGTTTTAA‑AGAAACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaa                                                                        <  1:1101858/233‑1 (MQ=255)

                                                                                                                                                       cctgtgccGGTGGTTGCTGTAATATAGAAGACAAATGCGATTGAGATAAAACCCCC‑GGCGTTAATCGCAAGAAAGAAAGTCTTAAGCG‑GATTTTTCTTTGTTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  1:2063749/208‑1 (MQ=255)

                                                                                                                                                        gtgggccAGTGGTTGCTTTGATATAGAAGACGAATGCGATTGAGATGAAAACCCC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTTCTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  1:1092519/234‑1 (MQ=255)

                                                                                                                                                         tgtgccAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAtt                                                                     >  1:1369555/1‑239 (MQ=255)

                                                                                                                                                           tgccATTGGTTGGTTTGATAAAGAAGACAAATGGAATGAAGATAAAACCCCC‑GGCGGTAACCGCCAAAAAAAAAGTTTTAGGCG‑GATGTTTCGTTGTTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTg                                                                    <  1:277585/238‑1 (MQ=255)

                                                                                                                                                              cAGTGTTGGCTGGGAAATGGAAGGGGAATGGTATTGAGATAAAAGCCCG‑GGCGTTAATGCCCAAATGAAAGGTCTTAAGCG‑GTTTTTTCG‑TGTTTTATAATACTCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGATATACTAACAAAGCATTATAGATGAGAAATTGatat                                                                <  1:1864868/239‑1 (MQ=255)

                                                                                                                                                               aGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCg                                                                                                                                                                                                                       >  1:1552283/1‑89 (MQ=255)

                                                                                                                                                               aGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGAtata                                                               >  1:413788/1‑239 (MQ=255)

                                                                                                                                                                 tGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAgg                                                                                                                                                  >  1:1624013/1‑154 (MQ=255)

                                                                                                                                                                  ggTTGCTGTAATAAAGAAGACGAAGGCAATTGA‑AAGAAAACCCCGGGCGGTAATCG‑CAGATAGAAAGTCTGAAGCG‑GTTGTTTCGTTGCTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGAt                                                            <  1:228352/238‑1 (MQ=255)

                                                                                                                                                                   gTTGCTGTGATATAGAAGACGAATGCGCTTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGCATTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTTACAATAACTCTCTCTTTATTAAAGCGGAGCAGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATc                                                           >  1:1857953/1‑239 (MQ=255)

                                                                                                                                                                      gCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCata                                                        >  1:1183563/1‑239 (MQ=255)

                                                                                                                                                                           gATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGAACAAAAAGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            >  1:1490624/1‑198 (MQ=255)

                                                                                                                                                                             tataGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGAAAGAAAGTCTTTAGGG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTTCCTCTTTATTAAGTCGGCGACGAGAAACTAACAAAACATTATAGATGAGAAAATGATATAGATCatattacg                                                   >  2:790997/1‑233 (MQ=255)

                                                                                                                                                                             tataGAAGAAAAAGGCGATTAAGATAAAACCACG‑GGCGGTATTCGCCAGATAGAAAGTCTTAAGCG‑GTTGTTTTTTTTCTTTAA‑AAACCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1694804/239‑1 (MQ=255)

                                                                                                                                                                             taaaaaagaCGAATGCGTTTGAGATAAAAACACC‑GGCGGTAATCGCAAGATAGAAATTCTTAAGCG‑GTTGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1433164/234‑1 (MQ=255)

                                                                                                                                                                                  aagaCGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGa                                                             >  1:28070/1‑222 (MQ=255)

                                                                                                                                                                                  aagaCGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAAATAGAAAGTCTTTAGCG‑GGTGTTTCGTTGCTTTTT‑AGACACCCGCCTCTTCGGGCACTTTGGCCATTG‑CAGCATGAAGTAAAAGATCCAAAGGGTTGTCCGCTTTCACACTCACTCTCTCTTTATTAAGTCGGCGACGAGATACTCACAAAACACTATAAATAATAAATTGATATAGATCATATCTCGCCTTGCt                                            >  2:951005/1‑239 (MQ=255)

                                                                                                                                                                                    gaCGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCCCTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          >  1:1928532/1‑239 (MQ=255)

                                                                                                                                                                                    gaCGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAAtt                                                                     >  1:757785/1‑212 (MQ=255)

                                                                                                                                                                                    gaCGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGa                                                             >  1:1906881/1‑220 (MQ=255)

                                                                                                                                                                                    gaCGAATGCGATTGAGATGAAAACACC‑GGCGGGAATTGCCAAATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGAAACCCGCCTCTTCGGCCACTTTGGGCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTCACAAAACATTATAAATAAGAAATTGATATAGATCATATCTCGCCTGGCCta                                          >  2:1507935/1‑239 (MQ=255)

                                                                                                                                                                                      cGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTtata                                        >  1:308063/1‑239 (MQ=255)

                                                                                                                                                                                      cGAATGCGATTGAGATGAAAACACC‑GGCGGGAATCGCCAGATAGAAAGGCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTtata                                        >  1:1786432/1‑239 (MQ=255)

                                                                                                                                                                                       gAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCCTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CCGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTTACTCTCTCTTTATTAAGACGGCGACGAGATACTAACAAAGAATTATAGATGTGAAATTTATATAGATCATATCTCCCCTGGCTtttaa                                       >  2:1433164/1‑238 (MQ=255)

                                                                                                                                                                                       gAATGCGATTGAGATGAAAACACC‑GGCGGTAATCACCAGATAGAAAATCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAAATCAAAAGGGGTTGCAGCTTTCAAACTAACTCTCTCTTTATTAATTCGGCGACGAGATACTAACAAAACATTATAGATTAAAAATTGATATAGAACATAACTCACCTGGCTTATAg                                       >  2:189009/1‑239 (MQ=255)

                                                                                                                                                                                         aTGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTTACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAgg                                      >  1:1714423/1‑238 (MQ=255)

                                                                                                                                                                                          tGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGc                                     >  1:363532/1‑238 (MQ=255)

                                                                                                                                                                                            cGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGGTAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAActctct                                                                                                                        >  1:274473/1‑153 (MQ=255)

                                                                                                                                                                                            cGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGGTTCGTTGCTTTAT‑AGACACCCCCCCCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACCCTAAATCTCTCTTTATTAAg                                                                                                              >  1:1248387/1‑163 (MQ=255)

                                                                                                                                                                                              aTTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAct                                                                                                                            >  1:1392061/1‑147 (MQ=255)

                                                                                                                                                                                                  agagaaaacCACC‑GGCGGTAATCGCCAGAAAGAAGGTCTTAGGCG‑GATGTTTCGTTGCTTTAA‑AAACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACt                             <  1:2075347/233‑1 (MQ=255)

                                                                                                                                                                                                    ataaaaaCACC‑GGCGGTAACCGCCAGATAAAAAGTCTTAAGCG‑GATGTTTTGTTGTTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  1:2097269/172‑1 (MQ=255)

                                                                                                                                                                                                     tGAAAACCCG‑GGCGTTAATCGCCAGATAGAAAGTCTTAAGCG‑ATTTTTTCGTTGTTTAAT‑AGCCACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  1:1034940/239‑1 (MQ=255)

                                                                                                                                                                                                      aaaaccccc‑GGCGGAAATCGCCAGATAGAAATTCTAAAGCG‑TTTGTTTCGTGTTTTTAA‑AAACCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCAt                        <  1:790997/232‑1 (MQ=255)

                                                                                                                                                                                                       aaaccccc‑GGGGTTAATCCCCAGATAAAAAGTCTTAAGCG‑GTTTTTTCTTTCTTTAAT‑AGCCCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCAt                        <  1:1681179/232‑1 (MQ=255)

                                                                                                                                                                                                       aaaaCACC‑GGCGGTAATCGCCAGATAGAAAGTCTTTAGCG‑GGTGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGGCATTG‑CAGCAGGAAGGAAAAAATCAAAAGGGTTGTCCGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGAAACTAACAAAGCATTATAGATTAGAAATTGATATAGATCATATTCCGACTGGCTTATAGTCCCGAAACTCGCATg                       >  2:425892/1‑239 (MQ=255)

                                                                                                                                                                                                            acC‑GGCGGTAACCGCAAGATAGAAATTCTTAAGCG‑GTTGTTTCGTTGCTTTAA‑AGCCACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGtttt                  <  1:951005/239‑1 (MQ=255)

                                                                                                                                                                                                            acC‑GCCAGTAATCGACAGATAGAAAGTTTTAGGCG‑GTTTTTGCTTTGTTTAAT‑ACACCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCACGCCTGGCTTATAGGCCCGTAACTCGCATGGtttt                  <  2:413788/239‑1 (MQ=255)

                                                                                                                                                                                                             ccgggggatAATCGCCAGATAGAATGTCTTAAGGG‑GTTGTTTCTTTCTTTAAT‑AGCCCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGtttt                  <  1:1507935/231‑1 (MQ=255)

                                                                                                                                                                                                              c‑ggcggTAACAGCCCGATAAAAAGACTTAAACG‑GATGTTTCGTTGCTTTAT‑AGAAACCCGCCTCTTCGGCCACTTTGGCCATTGCCAACA‑GAAGGAAAAGATCAAAAGAGTTGTCCGCTTTCAACCCAACTCTCTCTTTATTAAGGCGGCGACGAGATACTAACAAAACATttta                                                                                >  2:2097269/1‑175 (MQ=255)

                                                                                                                                                                                                                 gcggTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACCTTGGCCATTT‑CAGCAGGAAGTAAAAAATCAAAAGGGGTGTCCGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGCCGAGAtaataa                                                                                            >  2:1207008/1‑161 (MQ=255)

                                                                                                                                                                                                                       accgcaaaaaaGAAAGTTTTAAGGG‑TTTTTTTTGTTTTTTTAT‑AAACTCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:257246/229‑1 (MQ=255)

                                                                                                                                                                                                                       aTCGCCAGATAGAAATTCTTAAGGG‑GTTTTTTCTTTGTTTTAT‑ACACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:448847/239‑1 (MQ=255)

                                                                                                                                                                                                                       aTCGCAAGATAGAAAGTCTTAAGGG‑GATTTTTCTTTGTTTTAA‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:189939/239‑1 (MQ=255)

                                                                                                                                                                                                                             aGATAGAATTTTTAAGGCG‑TATTTTTCGTTTTTTTAA‑AACCCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCACGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:1965657/239‑1 (MQ=255)

                                                                                                                                                                                                                             aGATAGAAGTTCTAAGGGG‑GTTGTTTCGTTGCTTTAG‑AGACCCCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:189009/239‑1 (MQ=255)

                                                                                                                                                                                                                                         ttAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAg                                       >  1:1764380/1‑190 (MQ=255)

CAACTGACCCCAGGTGATGCGCCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATGCCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGTGCCAGTGGTTGCTGTGATATAGAAGACGAATGCGATTGAGATGAAAACACC‑GGCGGTAATCGCCAGATAGAAAGTCTTAAGCG‑GATGTTTCGTTGCTTTAT‑AGACACCCGCCTCTTCGGCCACTTTGGCCATTG‑CAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_007779/954559‑955017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`