Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,120,992	A→T	85.3%	L435Q (CTG→CAG)	wzxC ←	M‑antigen undecaprenyl disphosphate flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,120,992	0	A	T	85.3%	112.0 / 15.1	49	L435Q (CTG→CAG)	wzxC	M‑antigen undecaprenyl disphosphate flippase
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base T (27/13);  total (32/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

ACGGATAAGGCGTTTTACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTA  >  NC_007779/2120759‑2121225                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          gtcgagaaagCGGTTTACCCCGCATCCCGCAACGGTTTTTGGAACCCGAAACGCGAACCCCCCCCCGCCGTAAAAACCTTTTTCATTTTTCCG‑TGCCACAAAAACTGACGCTCCCCTCCCCCCCCCAGCGGAGGGCGCGACAGCACAACCACCACCCCAAACGCCGCCCCCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTgcg                                                                                                                                                                                                                                        <  2:232454/229‑1 (MQ=255)     aTAAGGCGTTTTACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAGACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATa                                                                                                                                                                                                                                    >  1:1880598/1‑237 (MQ=255)     aTAAGGCGTTTTACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATaa                                                                                                                                                                                                                                   >  1:840600/1‑238 (MQ=255)            gTTTTACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGacca                                                                                                                                                                                                                            <  1:1127336/238‑1 (MQ=255)              tttACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGAccacca                                                                                                                                                                                                                         >  1:1628544/1‑239 (MQ=255)               ttACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGAccacca                                                                                                                                                                                                                         >  1:1546703/1‑238 (MQ=255)                                      gTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACg                                                                                                                                                                                                 >  1:404501/1‑239 (MQ=255)                                      gTCGAAACCGAAACCACCAACTCCCCCCGCCCGT‑AAAAGCATTTCCATTTTTCCGCTGCGAC‑AAACCTGACGCTTCACTGCACCCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCCCCCCCGTGGTTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAACgaacaaaacaaac                                                                                                                                                                                                 <  2:1100902/239‑12 (MQ=255)                                               aacacagccAACTCGCCCCCCCCGG‑AAAGCAAGGTTTATTTTTTCCCGGCCAC‑AAACCTGACTCTACCGTTCCCCCACAAGGCGAGGGCGCGACAGCCAAAGCATCCCCACAACCGCCACCACCCCCGTGTCTATTTGCCCCGCCAGCAGCATCCCCAGCGCCAGTTGCCATTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATa                                                                                                                                                                                        <  2:1076307/231‑1 (MQ=255)                                                  aacgcccccccACCCCGCCCTT‑AAAAGCAGTTTCGTCTATCCGCTCCGAA‑ACAACTGTCCCTTCACTTCCACCGCCAGCGGATGGCCCGACAGCCCAACAATCCCCACAACCCCCCCACCCCCCGTGGCTATTGCCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAAc                                                                                                                                                                                     <  2:79680/229‑1 (MQ=255)                                                      cgtcacgcccccTCCCGA‑ACAGGCTTTTCAATTTTTTCCAGCCGAA‑AAAACGAACGCTCCCCTCCCCCCCCCCGCGGGTGGCGCGACGCCACAACCATCCCCACAACCCCCTCACCCCCCGTGGTTATTGGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCGTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAg                                                                                                                                                                                 <  2:764883/231‑1 (MQ=255)                                                                   cccgT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATaaa                                                                                                                                                                                      >  1:829022/1‑221 (MQ=255)                                                                   cccgT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGa                                                                                                                                                                    >  1:488569/1‑239 (MQ=255)                                                                   cccgT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGa                                                                                                                                                                    >  1:462410/1‑239 (MQ=255)                                                                                               tGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAatc                                                                                                                                         >  1:715436/1‑239 (MQ=255)                                                                                                         aCTGCCGCTTCACTGCCACCACCACGGCACGGCGCAACAACAAAATCCACCCCCAAAACTCCACCCCCCCCTTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCTCCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAg                                                                                                                                <  2:1675917/239‑1 (MQ=255)                                                                                                                  tCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGc                                                                                                                                                                      >  1:1748326/1‑192 (MQ=255)                                                                                                                  tCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGa                                                                                                                       >  1:872466/1‑239 (MQ=255)                                                                                                                              ccaGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATaa                                                                                                                                                                 >  1:151306/1‑185 (MQ=255)                                                                                                                                      tGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAg                                                                                                                                                      >  1:1236821/1‑188 (MQ=255)                                                                                                                                                           accacAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAat                                                                                                                                          >  1:2050849/1‑179 (MQ=255)                                                                                                                                                           accacAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGAc                                                                              >  1:346589/1‑239 (MQ=255)                                                                                                                                                            ccacAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATaa                                                                                                                                                                                                                                   >  1:330018/1‑89 (MQ=255)                                                                                                                                                              acaAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGcc                                                                           >  1:1492080/1‑239 (MQ=255)                                                                                                                                                                aaaCGCCAGCCCCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGacca                                                                                                                                                                                                                            <  1:146636/92‑1 (MQ=255)                                                                                                                                                                aaaCGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGacca                                                                                                                                                                                                                            >  2:146636/1‑92 (MQ=255)                                                                                                                                                                aaaCGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGa                                                                         >  1:307348/1‑239 (MQ=255)                                                                                                                                                                     ccAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGAAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATcgc                                                                     >  1:1213228/1‑238 (MQ=255)                                                                                                                                                                       aGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTg                                                                                                              >  1:1664116/1‑195 (MQ=255)                                                                                                                                                                       aGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTg                                                                                                              <  2:1664116/195‑1 (MQ=255)                                                                                                                                                                                  ggtctttgccACCGCCAGCAGCAACCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCGGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGc                                                        <  2:94445/229‑1 (MQ=255)                                                                                                                                                                                  gactcgttGCACCGCCAGCGGCATCCCCACCGCCAGTTCCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGcc                                                       <  2:842012/233‑1 (MQ=255)                                                                                                                                                                                        ttGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCta                                                 >  1:348669/1‑239 (MQ=255)                                                                                                                                                                                        ttGCACCGCCAGCAGCATCCCCAGCCCCAGTTCCCCTTTCAGCACAATTCCCTGCGCATAACTGACCACCAGCGTCGCCAGCGAGAGATAACACGGCAGCCATACACTCCGGATGCACTGGGGATAACTGGAACCACGAACCGGTTTAAGCATCACGACGTAACACCGAAGGGTGTTGCTAATTTGCACCAGCAGGATGCCACGCGCGGCGCTGCTCGCGCCCGCCATCTGCGTACCt                                                  >  2:837606/1‑238 (MQ=255)                                                                                                                                                                                              cGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGccc                                                      >  1:172861/1‑228 (MQ=255)                                                                                                                                                                                              cGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGccc                                                      <  2:172861/228‑1 (MQ=255)                                                                                                                                                                                               gCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACg                                                                                                                                   <  2:1123679/150‑1 (MQ=255)                                                                                                                                                                                               gCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACg                                                                                                                                   >  1:1123679/1‑150 (MQ=255)                                                                                                                                                                                                           cccAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATaaa                              <  2:404501/239‑1 (MQ=255)                                                                                                                                                                                                               gcgcCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACt                                                                                                                           >  1:648366/1‑142 (MQ=255)                                                                                                                                                                                                               gcgcCAGTTGCCCTTTCAGCACAATGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACt                                                                                                                           <  2:648366/142‑1 (MQ=255)                                                                                                                                                                                                                                  cacaATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGa       >  1:149517/1‑239 (MQ=255)                                                                                                                                                                                                                                     attGCCCGGCGCATAACAGACTGCCAACGTCGGCAGCGAGAGATAAATCGGCATCCATAAACTCAGGATGTACTGGCGATATCTGGAACCAATATCCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAATCAAAGCGTGGCTCCGATCGCGCCCGCCATCTGCCCTTCTATAACAATTGCCGGAATAAACAGAAATGATTTGAATACGtt         <  2:1033898/232‑1 (MQ=255)                                                                                                                                                                                                                                       tGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGaa                                                                                                                      >  1:1325642/1‑123 (MQ=255)                                                                                                                                                                                                                                       tGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGaa                                                                                                                      <  2:1325642/123‑1 (MQ=255)                                                                                                                                                                                                                                       tGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTa  >  1:494704/1‑239 (MQ=255)                                                                                                                                                                                                                                       tGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTa  >  1:862298/1‑239 (MQ=255)                                                                                                                                                                                                                                       tGCCCTGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTa  >  1:1962708/1‑239 (MQ=255)                                                                                                                                                                                                                                       tGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTa  >  1:2118995/1‑239 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          ACGGATAAGGCGTTTTACGCCGCATCCGGCAACCGTTGTCGGAACCGAAAACAGCAACTCACCCCGCCCGT‑AAAAGCATTTTCATTTTTTCGCTGCGAC‑AAAACTGACGCTTCACTTCCACCACCAGCGGATGGCGCGACAGCACAATCATCACCACAAACGCCAGCACCCCCGTGGCTATTTGCACCGCCAGCAGCATCCCCAGCGCCAGTTGCCCTTTCAGCACAATGCCCAGCGCATAACTGACCACCAGCGTCGGCAGCGAGAGATAAAACGGCAGCCATAAACTCAGGATGTACTGGCGATAACTGGAACCAAGAACCGGTTTAATCATCACGAAGTAACTCAGAATGGTGTTGATAATTTGCACCAGCAGGAAGCCAAGCGTGACGCCGATCGCGCCCGCCATCTGCCCACCTATAACAATCGCCGGAATAAACAGAAATGTTTTGAATACGTTGAATTTA  >  NC_007779/2120759‑2121225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑