| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_007779 | 2,153,826 | G→T | 24.9% | intergenic (+43/+22) | Y75_RS10870 → / ← pphC | YegJ family protein/protein‑serine/threonine phosphatase PphC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 2,153,826 | 0 | G | T | 24.9% | 38.9 / 4.8 | 36 | intergenic (+43/+22) | Y75_RS10870/pphC | YegJ family protein/protein‑serine/threonine phosphatase PphC |
| Reads supporting (aligned to +/- strand): ref base G (20/6); new base T (6/3); total (26/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01 | |||||||||||
GTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAC > NC_007779/2153611‑2154050 | gTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATAACATTTATCCTTTTTTTGTTACCTATCCCAAAAAAGAAAACGACGAGCGAAAAGCAAAAATAATTTGCTAATTAACCGGTATATTTTTTTTTTTAAATTGTGGGTGGAT‑TTGTTTTTTTTATCCCCCACGAGaaaac > 1:1419068/1‑237 (MQ=255) tATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAAAAAGAAGATATTACAGACTGGATGTATAGGCGCGGGAAAAAAATTAACGGCGATTACTCCATAGACCCTTTATTGGCCCCCTAGCAAAAGGAAAAAGCCGACGAGAAAAGAGAAAAATTAGTTCACTAATCACCGGTTATA‑TTTATTTTTAAAACTGGGTGTTTAT‑TTTTTGTCTACAACCGCCATGAGAAactc < 1:218749/238‑1 (MQ=255) agggAGTGAAAAAACAAAAAAGAAGATTTTTCAGATGGGATGTATCCGCGGGGGGAAAAAATTTCGGGCGGTTATACATTTGATCTTTTTTTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCTCTAATCAGCCTTTATA‑TTTATTTTTAAACCGGGCGGTTAAA‑TTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa < 1:291434/236‑1 (MQ=255) aaaaACAAAAAAGAAGTTATTTCAGACGGGTTTTATACGCGCGGGAAAAAAATTTACGGCGTTTATACATTTGATCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGAGGAGATAAGAAAAAAATTAGTTCGCAAATCAGCCGTTATA‑TTTATTTTTAAAACGGGGGTTTAAT‑TTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatca < 1:385325/239‑1 (MQ=255) ttATCCCATTTATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGAAGAGCAAAGAAAAAAATTAGTTCGCTAACCAGCCTTTATA‑TTTATTTTTAAAACGGGCGTTTGAT‑TTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTc < 1:80078/230‑1 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTTAA‑TTGTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCTGGTAATAAATCGAgttg > 1:1495269/1‑239 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTTTTTAACCGCCATGAGAAACTCACTCTTCCCACCGCGCCAGGTCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCC‑GGGCAAAAAATGCTTTAAC‑AATTCAGGGAATAAAACGAgttg > 1:1501585/1‑239 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGCTGAA‑TTGTTGTCTTCAACCGCCAGCAGAAAATCACTCTGCCCACAGCGCCAGCGTCAACGTTTTATCATCATCCGTACGCACGTTTACCGCCGGGCT‑GGACAAAAACACCTTTTAC‑AATTTTGGTCATAAATCGAgttg > 1:633257/1‑239 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTTTTGTCTTTAAACGCCCTGAGGAAATCACTCTTCCCACAGCGCCAGTGCCAACGGTTTTTTATCATCCGCAAGCCCCTTTTCCCCCGGGCTGGGACAAAACCTCCTTTAAA‑AATT‑AGGTAATAAATctctccct > 2:80078/1‑232 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAgttgt > 1:163507/1‑239 (MQ=255) ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTTAACCGCCATGAGAAAATCCATTTGCCCAAAGGGCCAGTGCCAAAGTTTTATCAACAACTGTACACTCGTTTTCCCACGGGCG‑GGCAAAAAACTGCTTTACA‑AATTCAGGTAATAAATCGAgatgtt > 1:1367182/1‑237 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTTTAATCATCCGGACGCTTGGTTACCGCCGGGCC‑GGACAAAGACTGCTTTTAA‑CATTTAGGTAATAAATCGAGTTGTTCCTGc > 1:1265655/1‑239 (MQ=255) tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGGCAAAAAATGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct > 1:1819195/1‑238 (MQ=255) tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct > 1:1919259/1‑238 (MQ=255) cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGc > 1:866984/1‑125 (MQ=255) aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCTCGTTTTCCCCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCATTGa > 1:856001/1‑239 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGaaa > 1:976355/1‑239 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGGTTAA‑TTGTTTTTTTTAACCTCCATGAGAAAATCAATCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCCTCCGCCCCTTTACCCCCGGGCT‑GGGCAAAAAAAGCTTTTAC‑AAATCAAGGAATAAAACGCGTTGTTGCTTCGGTTCTGCCGCCCGTCCCTTTaaa > 2:1582856/1‑239 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGGTGGA‑TTGTTGTTTTCAACCGCCATGAAAAAATCACTCTGCCCCCAGCGGCAGTGCCCACGTTTTTTTATCATCCGTACGCTCCTTTACCGCCGGGCT‑GGGCAAAAACTGCTTTAACAAATCCAGGAAAAAAATC‑AGTTGTTCCTGCGTTGGTTCCCCCCAGCCATTGaaa > 2:483453/1‑239 (MQ=255) aaaTTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGTA‑TTTTTGTTTTTAAACACCAAGAGAAACTCACACTCCTCCAAGTCCCAGCGTCCACGCTTTATAATCATTCCCACGCTCCTTTATCTCCGGGGT‑GGCAAAAAAAAGCTTTAAC‑AACTAACGCAAAAAAACGAGGTATTCCTGCGTTTCTGGCGCCCGTCCAGTCAAAAAAGGGGGAAAAAAATGcaca > 2:101707/1‑239 (MQ=255) tAGTTCCTTAACCAGCCTTTATT‑TTTTTTGTTAAACCGGGCGTTTGAT‑TTGTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag < 1:126602/239‑1 (MQ=255) gTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga > 1:102000/1‑239 (MQ=255) aaccaccttttATT‑TTTTTTGTTTAACCGGGGGTTTATT‑TTTTTCTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt < 1:1312561/230‑1 (MQ=255) caccctttatttttttttttAACCGGGCGGTTAAATTTTTTGGCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTGCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCa < 1:552413/229‑1 (MQ=255) ccGTTATA‑TTTATTGTTTAAACTGGCGGTTTAA‑TTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTTAC‑AATTCCGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAAGCACATGAGGAGAATTATc > 1:414791/1‑234 (MQ=255) ccGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAACCGCCATTAGAAAATCAATCTGCCCACAACGACCATGGCCACGTTTTATTATCCTCCGTACGCCCGTTTACCCCCCGGCT‑GGACAAAAACTCCTTTAAC‑AATTCAGGTAATAAATCGAGTTTGTTCTTCGCTGCTGCCGCCCGTCCATTGAAAAACCGGGGAAAGAAAGGCACATGAAGAGAATTATCCAGCa > 1:1902415/1‑239 (MQ=255) tcttttttttttttGTTTAAATCTGCGTTTAAT‑TTGTTGCTTTACCCCGCCAGGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCTTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGATTTGTTCCTGCTTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa < 1:2109455/231‑1 (MQ=255) ttATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCCGGCT‑GGACAAAAAATGCTTTTAA‑AATTCAGGTAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCATTTAAAAAAGGGGTAAAGAAAAGCAAATGAGGAGAATTATCCAGCatat > 1:1313719/1‑239 (MQ=255) attGTTTAAACTGGCGGTTGGA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACACCGCCAGTGCCAACGTTTTATCATCATCCGTAAGCTCGTTTACCGCCGGGCT‑GGGCAAAAAATGCTTTAAC‑AATTAAGGTAATAAAACGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAAGCACATGAGGAGAATTATc > 1:94919/1‑223 (MQ=255) attGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGGAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc > 1:1500385/1‑239 (MQ=255) tGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAAACGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGGTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg > 1:1822572/1‑239 (MQ=255) tGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGGAATaaa > 1:1632022/1‑147 (MQ=255) gTTTAAACTGGCGGTTGAA‑ATGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCCACGTTTTATCATCATACGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGACTTAAC‑AATTTAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCAttt > 1:1292963/1‑181 (MQ=255) gCGGTTGAA‑TTTTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa < 1:1843128/238‑1 (MQ=255) ggtttGAT‑TTTTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc < 1:483453/235‑1 (MQ=255) cGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAg > 1:219897/1‑127 (MQ=255) | GTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATA‑TTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCT‑GGACAAAAACTGCTTTAAC‑AATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAC > NC_007779/2153611‑2154050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |