Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 220,098 G→A 10.4% intergenic (‑103/+15) rcsF ← / ← metQ Rcs stress response system protein RcsF/methionine ABC transporter substrate‑binding lipoprotein MetQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779220,0980GA10.4% 414.3 / 9.6 149intergenic (‑103/+15)rcsF/metQRcs stress response system protein RcsF/methionine ABC transporter substrate‑binding lipoprotein MetQ
Reads supporting (aligned to +/- strand):  ref base G (87/45);  new base A (2/15);  total (89/60)
Fisher's exact test for biased strand distribution p-value = 2.32e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTG  >  NC_007779/219863‑220333
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
gcggcgCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGaaa                                                                                                                                                                                                                                               <  1:2129/239‑1 (MQ=255)
 cggcgCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑c                                                                                                                                                                                                                                             >  1:468343/1‑239 (MQ=255)
               ttGCAGGCTTCGCTTTCAGCTGAGGTGCAGATCTTTGAACGGGTTCGGCAGGGGATCTGCATAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  1:1065252/239‑1 (MQ=255)
               ttGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  2:591794/239‑1 (MQ=255)
               ttGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  1:1341661/239‑1 (MQ=255)
               tggCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGTGGATCTGCTTAACATGGAACAGCCGCTTATCATGAGTGCTACTAAACAGAGCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  1:1468339/237‑1 (MQ=255)
                tGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAcc                                                                                                                                                                                                                             >  2:941742/1‑239 (MQ=255)
                      cTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGcc                                                                                                                                                                                                                         <  1:138880/237‑1 (MQ=255)
                               cGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCCACCTTT‑AACA‑‑GCt                                                                                                                                                                                                            >  2:32765/1‑238 (MQ=255)
                                           aGTGCTTTGACCGGGTTCGACGGGGGATCTGCTTACCATGGACCAGCGGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGAACAAAACGTCAATATTGAAAC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCGAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAac                                                                                                                                                                                                <  1:942463/238‑1 (MQ=255)
                                                         gTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGaaa                                                                                                                                                                                                                                               >  2:1346627/1‑182 (MQ=255)
                                                           tCGACAGGGGATCTGCTTAACATGGAACAGCCGCTAAGAATGAGTGCAAATAAACAGATCGGAAAAGCACGCATAGTATTTCCTCAATGTAGGAAAAAAACGACAATATAAAATC‑AGGGGCTTGT‑AAAAATGACAAGAGGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGAAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCtt                                                                                                                                                                                <  1:303363/238‑1 (MQ=255)
                                                                aggagaatggCTAAACATGAACCTGCCGCTTGTCAGAGGTGCAAATAAACAGATGGAAAAAGCACGATTAGTATTTCAAAAGTTAATAATAAAAACGCCATTATTAAATA‑AGGAGTTTAA‑AAAAATGAAAAGCCGGCAAAGCCCCCTCCCTG‑AATA‑ATAT‑AACAAATTGTGAAAC‑CAGCCTAAAAT‑TTCCCACCCTTA‑AACA‑‑CCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTaaa                                                                                                                                                                          <  2:173664/230‑1 (MQ=255)
                                                                       cTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              >  1:1973319/1‑183 (MQ=255)
                                                                       cTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  2:1973319/183‑1 (MQ=255)
                                                                         gCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑CAAAATGACAAGACAGGCAAGCGCCAGTCCTG‑AATG‑ATAT‑TACAATTTGTGGAAA‑CAGCCTAAACA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTAGCTGCGTCGTAAACTTCGTCAg                                                                                                                                                                 >  2:1332648/1‑239 (MQ=255)
                                                                              aCAGGGACCACCCGGTTAGCATGTGCGCTACTAAACAGATCAGAAAACCACGCATAGTTTTTCATAAATGTATAATCAACGCCTAACTGTTGAAAC‑AGGAGTTTGA‑AAAAACAAAAAGCGGGGACGGCCCCCCTCCGA‑CATG‑TACT‑TACAACTTGTCAAAA‑CA‑CCTAAAAATTTTCCACCCTTT‑ACCA‑‑GCCCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGa                                                                                                                                                            <  2:94931/239‑1 (MQ=255)
                                                                                         ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTaaa                                                                                                                                                                          >  1:1892323/1‑214 (MQ=255)
                                                                                         ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGa                                                                                                                                                  >  1:94931/1‑238 (MQ=255)
                                                                                                    gAGTGCTACTAAACAGATCGGAAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGTTTGA‑AAAAATGAAAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCa                                                                                                                                      <  1:1765695/239‑1 (MQ=255)
                                                                                                       tGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGt                                                                                                                                   >  1:1069423/1‑239 (MQ=255)
                                                                                                              aaaCAGATGGATAAAGCCTGCATATTTTTTCTACAATGTATAATAAATGAGACATTATTAAATA‑AGTAGCTTTA‑AAAAAAGAAAAAACGGGAAAGCGCGC‑TTCTG‑AAAT‑AAATATACAATTTGTGAAAC‑AATCCAAAAAT‑TTCCCCGATTAT‑AACA‑‑TCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGc                                                                                                                            <  2:129556/239‑1 (MQ=255)
                                                                                                               aacagttgtgaaaaGCACGCAAGGTATTCCCTAAATGTATGAAAAAAACGACAATATTGAACA‑AGGAG‑TTGTAAAAAATGAAAAGACGGGAAAGCACCCTTCTGT‑ATTT‑ATAT‑TAAAATTTGTGGAAA‑CAGCTAAAAAA‑TTCCCGGCCTTT‑AACG‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCg                                                                                                                           <  2:468343/228‑1 (MQ=255)
                                                                                                                 cAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGa                                                                                                                                                                >  1:834236/1‑200 (MQ=255)
                                                                                                                 cAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCg                                                                                                                           >  1:321441/1‑237 (MQ=255)
                                                                                                                    aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTAc                                                                                                                                                                                                                              <  1:1868220/138‑1 (MQ=255)
                                                                                                                    aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCttt                                                                                                                      >  1:286919/1‑239 (MQ=255)
                                                                                                                    aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCttt                                                                                                                      >  1:1482258/1‑239 (MQ=255)
                                                                                                                    aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTAAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAg                                                                                                                                                                 <  1:859579/196‑1 (MQ=255)
                                                                                                                    aTCGGTAAAACACGCAAAGTATCATCTCAATCTATGATCACAACGTAAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAAACGGATAAGCGCACGACCTG‑AATG‑ATAT‑TAAAAATTGTGAAAA‑AAGCCTAAAAA‑TTACTCACGTTT‑AAAA‑‑GTTCCTCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCACACTGATACTCCTCGACGAATTTCTCCACGTTCTCGCCGTCttt                                                                                                                      >  2:1997776/1‑239 (MQ=255)
                                                                                                                              cACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTc                                                                                                             >  1:771882/1‑238 (MQ=255)
                                                                                                                              cACGCATAGTATTTCCTCAATGTATGATCAAAACGGCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTACGCCGTTAAACACTTTGTTTGCTGCATCGTAAACTTCGACAGACTGATATGCCGGGACGAATTTCTTCACGTTCTCGTCGTCTTTGTTATCTTc                                                                                                             >  2:100735/1‑238 (MQ=255)
                                                                                                                                   aTAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCAcgcg                                                                                                        >  1:1591502/1‑238 (MQ=255)
                                                                                                                                         ttcccaaaaTGTATAAAAAAAACGTAAATAAT‑AATCAAGGACTTTAA‑AAAAGTGACAAGGC‑GGCAAACCCCCCGCCTG‑AAAGAATAT‑TCAAAATTGTGAAAC‑CACACAAAAAA‑TTCCCAGCCTTA‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGAt                                                                                                 <  2:1591502/232‑1 (MQ=255)
                                                                                                                                           tCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGc                                                                                                                                                       >  1:1942239/1‑183 (MQ=255)
                                                                                                                                           tCCTAAATGTATGATCAAAACGTAAATATTGAATC‑AGGAGTTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTCCCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCa                                                                                               <  1:32765/239‑1 (MQ=255)
                                                                                                                                            ccTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGa                                                                                                                                                                >  1:1699567/1‑173 (MQ=255)
                                                                                                                                            ccTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAg                                                                                              >  1:1738415/1‑239 (MQ=255)
                                                                                                                                               cAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAGCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGtt                                                                                           >  2:1671279/1‑239 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTTAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCTTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGtt                                                                                           <  1:737964/237‑1 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCTGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGtt                                                                                           >  2:737964/1‑237 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGcc                                                                                                                                                                                                                         >  2:1215847/1‑114 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTccg                                                                                                                                                                                                         >  2:1198890/1‑127 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTccg                                                                                                                                                                                                         <  1:1198890/127‑1 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  1:717698/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  1:1687290/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  1:1600973/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  1:1346391/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  2:621425/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGttt                                                                                          >  1:1468983/1‑238 (MQ=255)
                                                                                                                                                 aTGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGCCGTCTTTGTTATCTTCACGCTTCACGATTAGGttt                                                                                          >  2:1865146/1‑238 (MQ=255)
                                                                                                                                                       gATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGt                                                                                            >  1:1276019/1‑230 (MQ=255)
                                                                                                                                                       gATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTtacgtacg                                                                                   >  1:1352603/1‑239 (MQ=255)
                                                                                                                                                       gATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTtacgtacg                                                                                   >  1:1742706/1‑239 (MQ=255)
                                                                                                                                                         taaacacaacAATATTAAATC‑AGAGTTTTAA‑AAAAATAACAAGCCGGCAAAGCGCCCGACCAG‑AATG‑AAAT‑TCAAAGTTTTGAAAA‑CATCATAAAAT‑TTCCCAGCTTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg                                                                                 <  2:1600973/230‑1 (MQ=255)
                                                                                                                                                         tCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg                                                                                 <  1:91772/239‑1 (MQ=255)
                                                                                                                                                         tCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg                                                                                 <  1:621425/239‑1 (MQ=255)
                                                                                                                                                         ataggaacaagatatTGAATA‑AGGATATTGA‑AAAAACGACAAAGCGAG‑TACCCCCCCTCCTGTATTA‑ATAT‑AAC‑AATTGAGAAAACCAGCTAAAATA‑TTCCCCGCCTTT‑AACA‑‑CCCCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg                                                                                 <  2:1467960/228‑1 (MQ=255)
                                                                                                                                                            aaaCGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCAcgc                                                                                                         >  1:91114/1‑212 (MQ=255)
                                                                                                                                                            aaaCGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACgggg                                                                                >  1:572643/1‑237 (MQ=255)
                                                                                                                                                            aaaCGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACgggg                                                                                >  1:199600/1‑237 (MQ=255)
                                                                                                                                                                  cAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAac                                                                                                                                                                                                >  2:788489/1‑119 (MQ=255)
                                                                                                                                                                  cAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAac                                                                                                                                                                                                <  1:788489/119‑1 (MQ=255)
                                                                                                                                                                         gAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCTTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAGCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaa                                                                 <  1:1671279/239‑1 (MQ=255)
                                                                                                                                                                          aaTC‑AGGAGCTTGT‑AAAAATGACCAGCCGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTCAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCtt                                                                    >  1:1558741/1‑235 (MQ=255)
                                                                                                                                                                          aaTC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaac                                                                >  1:1304089/1‑239 (MQ=255)
                                                                                                                                                                          aaTC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaac                                                                >  2:2025916/1‑239 (MQ=255)
                                                                                                                                                                          aaTC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTc                                                                   >  2:237505/1‑236 (MQ=255)
                                                                                                                                                                          aaTC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTA‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTc                                                                   <  1:237505/236‑1 (MQ=255)
                                                                                                                                                                                ggAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTtacg                                                                                       >  1:995545/1‑211 (MQ=255)
                                                                                                                                                                                      tGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTtgct                                                                                                                                                                                    <  1:559446/112‑1 (MQ=255)
                                                                                                                                                                                      tGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTAt                                                                       >  1:290427/1‑221 (MQ=255)
                                                                                                                                                                                            aaaTGACAAGACTGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTAACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTc                                                >  2:1762185/1‑239 (MQ=255)
                                                                                                                                                                                                   aaGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGa                                                                                                                                                  >  1:65141/1‑134 (MQ=255)
                                                                                                                                                                                                                gcCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATa                                                         >  1:612714/1‑210 (MQ=255)
                                                                                                                                                                                                                 ctcGTCCTA‑AATA‑ATAT‑TACAAATTGTGAAAA‑CAGCTTAAAAA‑TTACCAGCCTTA‑AACT‑‑GCTCCGCCGTTAAACGCTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                           <  1:287469/237‑1 (MQ=255)
                                                                                                                                                                                                                  ccGTGCGG‑AATG‑ATAA‑TACAACTTGTGAAAA‑ACCCCT‑‑AAA‑TTTCCCCCTTTT‑AAAAACGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggc                          <  1:100735/239‑1 (MQ=255)
                                                                                                                                                                                                                   cgctgctaaaTG‑ATAT‑TGAAAGTTGTGGAAT‑CGGTGT‑AAAA‑TTGTCTGCCTTTGAACA‑‑GCTCCGCCGTTAAACACGTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTGCGGGGACTCTTTATCGTCAACAGAGATACCGTCTGTCGCCGGGGTCAGGCCAATctggc                          <  2:219149/231‑1 (MQ=255)
                                                                                                                                                                                                                       ctgaaaTG‑ATAT‑TA‑AAATTGTTGAAACCAGGTTAAAAA‑TTCCCTGCCATT‑ACCA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                      <  2:287979/235‑1 (MQ=255)
                                                                                                                                                                                                                           aaTG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                  <  1:2025916/239‑1 (MQ=255)
                                                                                                                                                                                                                           aaTG‑ATAT‑AACAAATGGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                  <  1:1365240/239‑1 (MQ=255)
                                                                                                                                                                                                                            agg‑ATAT‑TACAAATTGTGAAAC‑CAGCCTAAAAA‑TTCCCAGCTTTA‑AACA‑‑GCCCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                 <  1:1733645/237‑1 (MQ=255)
                                                                                                                                                                                                                            aTG‑ATAT‑GTCAAGTGGTGGAAA‑CAGCATAAAAA‑TCCCGACAATTT‑ACCA‑‑GCTCCGCCGTTAAACACTTTGTGTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                 <  2:1347553/239‑1 (MQ=255)
                                                                                                                                                                                                                             tG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt                >  1:999192/1‑239 (MQ=255)
                                                                                                                                                                                                                             attatat‑TAAAATTTGTTGAAA‑CAGACTAAAAA‑TTACCGGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTTCTTCGTCAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                 <  2:1857011/236‑1 (MQ=255)
                                                                                                                                                                                                                              atgaattaccAATTTGTGAAAC‑CAGCTTAAAAT‑TTCCCACCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                 <  1:1506746/230‑1 (MQ=255)
                                                                                                                                                                                                                                 tat‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTAt                                                                       >  1:1824752/1‑181 (MQ=255)
                                                                                                                                                                                                                                     tACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAtt          >  1:1198417/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGAc                                                                                                                                                 >  1:1893592/1‑102 (MQ=255)
                                                                                                                                                                                                                                       cAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa        >  1:428953/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTAACGCGTCACGATCAGGTGTACGTACggg                                                                                 >  2:1469596/1‑166 (MQ=255)
                                                                                                                                                                                                                                        aatgtgtgAAAT‑CAGTATAAACA‑TCCCCAGCCTGT‑ATCA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt       <  2:286919/235‑1 (MQ=255)
                                                                                                                                                                                                                                            tgtgGAAC‑CAGCCTAAAAA‑TTACCAGCCTTT‑AAAA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCCCGCGTCACGATCAGGTTtac                                                                                        >  2:1991574/1‑154 (MQ=255)
                                                                                                                                                                                                                                            tgtgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct                         >  2:987569/1‑217 (MQ=255)
                                                                                                                                                                                                                                            tgtgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggct                         <  1:987569/217‑1 (MQ=255)
                                                                                                                                                                                                                                            tgtgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGCCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATa      >  1:1527777/1‑236 (MQ=255)
                                                                                                                                                                                                                                            tgtgGAAA‑CAGCCTAAAAA‑TTAACAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGATTACTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGAGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGCGGACTCTTTATCTTCAACAAAAATACCTTCTTTCGCCCGAATCTGGCCAATCTGTCTGGCATACGTGGTATTGAtc      >  2:1780541/1‑235 (MQ=255)
                                                                                                                                                                                                                                              tgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGt                                                                                            >  2:883364/1‑148 (MQ=255)
                                                                                                                                                                                                                                              tgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAtt          >  1:1406108/1‑230 (MQ=255)
                                                                                                                                                                                                                                              tgGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTg  >  1:1763909/1‑238 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
GCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATC‑AGGAGCTTGT‑AAAAATGACAAGACGGGCAAGCGCCCGTCCTG‑AATG‑ATAT‑TACAAATTGTGGAAA‑CAGCCTAAAAA‑TTACCAGCCTTT‑AACA‑‑GCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTG  >  NC_007779/219863‑220333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑