Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,050	T→G	F263V (TTT→GTT)	clpA →	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,050	0	T	G	100.0%	221.4 / NA	62	F263V (TTT→GTT)	clpA	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/30);  total (32/30)

ATGGAGAATTTCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAAT  >  NC_000913/923768‑924333                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                            agggAGAATTTAACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCGACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGGTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATTTGGGGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGaa                                                                                                                                                                                                                                                                                        <  1:623504/286‑1 (MQ=255)      gaATTTCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGt                                                                                                                                                                                                                                                                                  >  1:25487/1‑289 (MQ=255)        aTTTCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAACTATCGCGGCGACGTTGAAACACGttt                                                                                                                                                                                                                                                                                >  1:693841/1‑289 (MQ=255)         tttCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGtttt                                                                                                                                                                                                                                                                               >  1:223878/1‑289 (MQ=255)          ttCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTAGGACTTACTCTCTCGATAGCGTTTCCCTGTTGGCGGGCACAATATTTCGCGGCGCCGTTTAAAAACGTTTTa                                                                                                                                                                                                                                                                              >  2:70419/1‑289 (MQ=255)                               cTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTgg                                                                                                                                                                                                                                                         >  1:217751/1‑289 (MQ=255)                                    gcgcgTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCGCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAg                                                                                                                                                                                                                                                    <  1:143945/289‑1 (MQ=255)                                     cgggTGGGGGGAATCGAACCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAg                                                                                                                                                                                                                                                    <  1:70419/285‑1 (MQ=255)                                          gggCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCCATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGacac                                                                                                                                                                                                                                               <  1:646323/288‑1 (MQ=255)                                          gggCGGAATCCACCCACTGATTGGTCGTGAGAAGGGGCGTGAGAGTGCTATTCAGGTGCTCTGCCGTCTCCGTAAAACCAACCCGCTGCTGGTGGGGGGATCTGGTGTCGGTAAAGCCGGGATTGCGGAAGGGCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGacac                                                                                                                                                                                                                                               <  1:32439/288‑1 (MQ=255)                                                  tCGACTCACTGATTGGTCGGGGGAAGGGGCTGGGTCGTGCTATTCCGGTTTTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAAGCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCAt                                                                                                                                                                                                                                      <  2:223878/289‑1 (MQ=255)                                                   cGACCAACTGATGTGTCGTGGGAAGGAGCTGGGGCGGTGTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATc                                                                                                                                                                                                                                     <  2:25487/289‑1 (MQ=255)                                                          gtgaggggTCGTGGTAAGGAGCTGGAGGGTGCTAGTCAGGTCCTCTGCCGTGGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGGCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGGACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTa                                                                                                                                                                                                                              <  2:304395/283‑1 (MQ=255)                                                              ttgattgggagaAGGAGCTGGTGCGTGCTAGTCAGGGTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGGAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGACGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATtg                                                                                                                                                                                                                           <  1:397780/280‑1 (MQ=255)                                                               tggtctggggacggAGCGTGAGGGTGCTTTTGAGGTTGTCTGCCGGGGCCGTAAAAAAAAACCGCTGCTGGTGGGGGGATCTGGTGTCGGTAGAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTAATCTCGCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGGTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATtgat                                                                                                                                                                                                                         <  2:696325/277‑1 (MQ=255)                                                                      gagaAGGAGGTGGGGCGTGCTATTCAGGATCTCTGCCGTGGCCGTAAAAACAACCCGCTGCTGGTGGGGGAAGCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTATTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                                                                                                                                  <  2:384058/289‑1 (MQ=255)                                                                       agaAGTAGCTGGAGCGTGCTAGTCAGGTTCTCTGCCGGAGCCGGAAAAACAACCCGCCGCTGGGGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                                                                                                                                  <  2:237536/288‑1 (MQ=255)                                                                       agaAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCTCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                                                                                                                                  >  1:632631/1‑288 (MQ=255)                                                                        taaGGAGCTGGGACAGTCTATTCAGGGTCTCTGCGGGTGCCGTAAAAACAACCCGCTTCTGGGGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGAGTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCca                                                                                                                                                                                                                 <  2:693841/287‑1 (MQ=255)                                                                            gAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTGGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGACCAACATATCGCGGAGACGTCGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGACCAGGCCACGAACAGCATCCTGTTACTTGATGAGTGCCAAACCa                                                                                                                                                                                                            >  2:196196/1‑289 (MQ=255)                                                                             ggctgggtggTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATc                                                                                                                                                                                                                   <  1:606066/272‑1 (MQ=255)                                                                               cTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTATCGATATCGGTTCTCTGTTAGCGGGCCCAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCCGGACACTAACATAATCCTGTTTATTGAGGAGATCCACACGATTa                                                                                                                                                                                                         >  1:54414/1‑289 (MQ=255)                                                                                       tGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAAAGTTTTAAAGCGTTGCCCAAGCAGCAGGAGCAGGACACTAACAGCATCCTGTTCATTGATGAGATCAATCCCATTATctcgc                                                                                                                                                                                                   >  2:606066/1‑283 (MQ=255)                                                                                                 gTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACATCATCCTGTTTATTGATGAGAACTACACCATTATCGGGTGGGGTGCAGCGt                                                                                                                                                                                       >  2:551693/1‑289 (MQ=255)                                                                                                      ctGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCtggt                                                                                                                                                                                  >  1:268602/1‑289 (MQ=255)                                                                                                              gCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCTATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCCGCCGGAGCAGGACACTATCAGCATCCTGTGTATTGATGAGATCCACACCATTATCCGTGCGGGTGCAGCGTCTGGTGGTCAgg                                                                                                                                                                           >  1:79452/1‑288 (MQ=255)                                                                                                                       acaacaCCCTGCCGGGGGTGGAAGCTGGGGTCGGTAAAACCGCGATTGAGGAAGGTCTTTCCTGGCGAATTTGTGAGGGGGATGTGCCGGAAGTGATGGCTGACTGTACTGTTTACTCGCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGc                                                                                                                                                                 <  2:279772/289‑1 (MQ=255)                                                                                                                           cccGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAGGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAAc                                                                                                                                                             <  1:33700/289‑1 (MQ=255)                                                                                                                                   tggtggGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCa                                                                                                                                                      <  1:439300/288‑1 (MQ=255)                                                                                                                                     gtggGGGAATTTGGTTGTGGGAAAGCCGGGTGGGCGGAAGGTCTTGGCTGGAGAATTGTTCAGGGCGAGGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCaaa                                                                                                                                                    <  2:552407/288‑1 (MQ=255)                                                                                                                                      tggGGGAATCTGGTTTCGGTAAACCCGCGATTGCGGAAGGTCTTGCCTGGCGAATTTTTCAGGGCGCTGTGCCGGTAGTGATGGCTGACTGTACGATTTACTCTCTCGCTATCGGTTCTCTGTTAGCGGGCACAAAATATCGCTGCGACGTTGAAAACCGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAAc                                                                                                                                                   <  1:651443/288‑1 (MQ=255)                                                                                                                                            aaTCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGc                                                                                                                                             >  1:192751/1‑288 (MQ=255)                                                                                                                                                                     tGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAAtt                                                                                                                   <  1:507991/289‑1 (MQ=255)                                                                                                                                                                        ggATGGTCTGGCCTGGCGAATTGTGAAGTGCGCTGTGCGGTAAGTGATGGCGGACTGTACGATTTAATCTCTCGATATCGGTGCTCTGTTAGCGGGCAAAAAATTACGCGGGTACGGTGAAAAACGTTTTAAAGCGTTGATCAAGCAGCTGGAGCAGGAGACTAACAGCAGCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGGCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGt                                                                                                                <  2:332191/289‑1 (MQ=255)                                                                                                                                                                                       gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAGGCGTTGCTCAAGCAGCTGGAGCAGGACACTAGAAACATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCGAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTGAGACAACATATCAg                                                                                                 >  1:281185/1‑289 (MQ=255)                                                                                                                                                                                       gCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCATCGTCTTGTGGTCAGGTCGATTCGGCTACCCTAACCAAACCGTTGCTCTCCAGCGGTCAAATTCGTTTACTTGGTTTCACAACATACCAg                                                                                                 >  2:116505/1‑289 (MQ=255)                                                                                                                                                                                        cGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGCTGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAgg                                                                                                >  1:585775/1‑289 (MQ=255)                                                                                                                                                                                                    ggCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACa                                                                                    >  1:369406/1‑289 (MQ=255)                                                                                                                                                                                                       gATGTGCCGGAAGTGCGTGCTGACTGTTAGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACAttt                                                                                 <  1:583758/289‑1 (MQ=255)                                                                                                                                                                                                                             aCTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTCAAGCGTTGCTCAAGCAGCGGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTCAACTTCGTGTAATTGCTTCGACAACCTACCAGGCTTTCAGCAACATTTTCGTACAAGAcctcacgctggc                                                           >  2:348317/1‑279 (MQ=255)                                                                                                                                                                                                                                             tctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTACAGaac                                           >  1:227896/1‑288 (MQ=255)                                                                                                                                                                                                                                              cgcgttagcGGTTAGCTGTGAGCGGGCACAAAATATCGCGGCGACGTTGCAAAACGTTTTAAAGCGTTGCTCAAGCGGCTTGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGACCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTGATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGaaaa                                          <  1:18836/281‑1 (MQ=255)                                                                                                                                                                                                                                               tcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATc                                                                                                                                                                                                                   >  2:9995/1‑119 (MQ=255)                                                                                                                                                                                                                                               tcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATc                                                                                                                                                                                                                   <  1:9995/119‑1 (MQ=255)                                                                                                                                                                                                                                                   tatCGGTTCTCTGTTAGCGGTCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGa                                      <  1:6642/288‑1 (MQ=255)                                                                                                                                                                                                                                                         ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTACGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGACAATTGATAtt                                  >  1:697716/1‑286 (MQ=255)                                                                                                                                                                                                                                                         ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAAAATTTTCGAGAAAGAACGTGCTCTGGCGCGTCGCTTCACGAAAATTGATCTTaat                               >  2:438666/1‑287 (MQ=255)                                                                                                                                                                                                                                                           ctctGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGATCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGa                             >  1:418739/1‑289 (MQ=255)                                                                                                                                                                                                                                                             ctGTTAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGaa                            >  1:139276/1‑288 (MQ=255)                                                                                                                                                                                                                                                                ttAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGCGGTCAGGTCGATGCGGCTAACCTAATCAAACCGt                                                                                                                                                <  1:284380/169‑1 (MQ=255)                                                                                                                                                                                                                                                                ttAGCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGCGGTCAGGTCGATGCGGCTAACCTAATCAAACCGt                                                                                                                                                >  2:284380/1‑169 (MQ=255)                                                                                                                                                                                                                                                                   gCGGGCACAAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTACAATTCGTGTAATTGGTTCGACAACCTATCAGGCGTTCAGCAACCTTTTCGAGAAAGACCGTGCTCTGGAGCGTCGCTTCCCGAAAATTGATATTAATGAACCTtcga                     >  2:558621/1‑289 (MQ=255)                                                                                                                                                                                                                                                                        cacaAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTAATGAACCGTCGCTCgaa                >  1:591565/1‑289 (MQ=255)                                                                                                                                                                                                                                                                        cacaAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATCGATATTACTGAACCGTCGATCgaa                >  1:484036/1‑289 (MQ=255)                                                                                                                                                                                                                                                                        cacaAAATAACGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCACAAGCAGCTGGAGCAGGACACTAACAGAATCCTGTATATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTAGATGCGGCTAACCTAATCAAACCGTTGCTACCCAGCGGTAAAATTCGTGTAATTGGTTCGATAACCTATAAGGATTTCCGAAACATTTTCGAGAAAGACTGTGCTCTGGCGCGTCGCTTACAGAAAATTGAAACTACTGAACCTtctatcgac                >  2:25007/1‑288 (MQ=255)                                                                                                                                                                                                                                                                        aacaAAATATGGCGGGGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGtcgatcga                 <  2:585775/287‑1 (MQ=255)                                                                                                                                                                                                                                                                          caAAATATCGCGGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCgaaga              <  1:196196/289‑1 (MQ=255)                                                                                                                                                                                                                                                                            aaaTATCGGGGGGAGGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGTAGCAGGACACTAACAGCATCCTGTGTATTGATGAGATCCACACCATTATCGGGGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGaaa            <  2:192751/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cgcgGCGACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTATCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATAAGGAGTTGAGCAACATTTTCGAGAAATACAGTGCTATGGCGCGCCGCTACCACAAAATTGATCTTACGAAACCGTCGATAGaaaaaaatctta      >  2:561878/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                     ggcgACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAAtt                                                                                                                   >  1:228126/1‑177 (MQ=255)                                                                                                                                                                                                                                                                                     ggcgACGTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAAtt                                                                                                                   <  2:228126/177‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gcgACGTTGCAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGAGACTAACAGCATCCTGGTTATTGATGAGATCCACACCATTATCGGTGAGGGTGCAGCGTCGGGTGGTCAGGTCGATGTGGCTAACCTAATCAAAACGTTGCTCTCCAGCGGTAAAATTCGTGTCATTGGTTAGACAACCTATCAGGAGTTAAGCAACATTTTCGAGAACGCCCGGTCTATGGCGCATCGCTTGCGGAAGATTGATAGTACTGAACAGCCCATCcaagctacgtgtacaat  >  2:330931/1‑276 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                            ATGGAGAATTTCACGACGAACCTGAATCAGCTTGCGCGCGTGGGCGGAATCGACCCACTGATTGGTCGTGAGAAGGAGCTGGAGCGTGCTATTCAGGTTCTCTGCCGTCGCCGTAAAAACAACCCGCTGCTGGTGGGGGAATCTGGTGTCGGTAAAACCGCGATTGCGGAAGGTCTTGCCTGGCGAATTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAAT  >  NC_000913/923768‑924333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑