Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C97.2% 235.8 / ‑1.5 73intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  major base C (39/30);  minor base T (1/1);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
*NC_0009134,296,3802.G95.9% 246.7 / 0.3 73intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (40/30);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTA  >  NC_000913/4296125‑4296652
                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                                   
cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGAc                                                                                                                                                                                                                                                      >  1:76967/1‑287 (MQ=14)
                                      ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                                                                                                                                                                              <  1:663528/289‑1 (MQ=19)
                                         ccgtCAACGCCTGATGCAACGCATGCCGCGTCTTATCAGGCCTACGCCCGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                                                                                                                                                                            <  1:152197/285‑1 (MQ=11)
                                                cGCCTGTTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                                    <  1:379949/289‑1 (MQ=255)
                                                cGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                                    <  1:12146/289‑1 (MQ=255)
                                                cGCCTGATGCAGAGCTTGCCGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                                                                                                                                    <  1:16006/289‑1 (MQ=255)
                                                       tGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                                                                                                                                                                              >  1:311366/1‑288 (MQ=255)
                                                            cGCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                        <  1:372452/289‑1 (MQ=255)
                                                            cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                         <  1:683885/288‑1 (MQ=255)
                                                            cGCTTGCCGCGTCTTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTAGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                         <  1:552663/288‑1 (MQ=255)
                                                                      ttcgtATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGGTCGGATAATGCGTTTATGCCGCATCCGACATCACCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                               <  1:237184/284‑1 (MQ=255)
                                                                      gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                               <  1:364552/288‑1 (MQ=255)
                                                                                    aCGCCAATCAGCTCAATATCCTTATTTAGCGTTATTTTTTAGGTCGTATAAGTCCTTTATCCCGCATCCTACATCAACGCCTGATGCCACGCTTAACGCATCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                                <  1:20529/289‑1 (MQ=17)
                                                                                     cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                                                                                                                                                <  1:481292/288‑1 (MQ=255)
                                                                                          gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCTTATCAGGCCTATCTTAATCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCAGGtt                                                                                                                                                          >  1:90028/1‑289 (MQ=255)
                                                                                          gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                                                                                                                           >  1:421866/1‑288 (MQ=255)
                                                                                           aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGTCAGCCAGAGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGT‑CGTTT‑‑ACGC‑C‑GCATCCGCCAACAATGCCTGTTGGTACGCTTGCCGTCTCTCTTCAGCCCTATCTTCACCGTTGGTTAATTTTTCACACTTTCCACCCCCGGTCACCAACCCTTCCAttccaatcagtttc                                                                                                                                                         >  2:698258/1‑276 (MQ=14)
                                                                                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCCTACGCCACACAGCGCAATAGCCTTATCTCGCGTGCTTTTGTATGTCGTATACTG‑CGTTTCCC‑‑C‑C‑GCCTTCGACATCATTTACTGATGAGACGTTTGCCTCTTCTTCTCATCCCTATCTTACACGTCGGTTAATTCTTCAACCTCTTCACTCCGAGTAACAAATCCTTCCATACacactacgtccaacc                                                                                                                                                     >  2:177796/1‑275 (MQ=9)
                                                                                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTACTTTTTCAACCTCTTACCACCCGGCATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                                      >  2:481292/1‑288 (MQ=17)
                                                                                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTCTCTTACACCTTGGTTAATTTTTCATACTCTTCACACCCGTTCTCAACCCCTTCcacacacataacgttcacc                                                                                                                                                      >  2:98486/1‑275 (MQ=14)
                                                                                               cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAACCGGTCTCAAACACTTCCATACAGccacggtctaacc                                                                                                                                                     >  2:203565/1‑277 (MQ=14)
                                                                                                       ccTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                               <  2:230801/255‑1 (MQ=255)
                                                                                                       ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                               >  1:230801/1‑255 (MQ=255)
                                                                                                         tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTTTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTGCAgcg                                                                                                                                            >  1:371212/1‑288 (MQ=255)
                                                                                                                                   aTAAGGCGTTAAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAAc                                                                                                                <  1:135838/289‑1 (MQ=255)
                                                                                                                                   aTAAGGCGGTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGttcttc                                                                                                                          <  1:247453/279‑1 (MQ=255)
                                                                                                                                    taagggtgttATCCCGCACCCGACATCACCGCCGGAGTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGGTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAAc                                                                                                                <  2:284131/280‑1 (MQ=21)
                                                                                                                                        gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACtttct                                                                                                           >  1:291157/1‑289 (MQ=255)
                                                                                                                                            ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                                                       <  1:606681/289‑1 (MQ=255)
                                                                                                                                             tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGTCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACATCCGTTATCAACCCCTTCCATACCGCTCAGGTCAATCCAGTCCAGCCCCTTTTG‑CTTGTCCTTCTCCCTAACCCCTTCTTCa                                                                                                        >  2:631164/1‑287 (MQ=18)
                                                                                                                                             tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAACGCTCAGTTTCAACCAGTGCCGCGCCTTTTG‑CTTGTTCTTCTCGATAAAAc                                                                                                                >  2:247453/1‑279 (MQ=255)
                                                                                                                                                  cGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ATGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGTTTCAACCAGTGCCGCCCCTTTTG‑CTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTa                                                                                                 >  2:95455/1‑289 (MQ=255)
                                                                                                                                                   gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTaa                                                                                                >  1:304620/1‑289 (MQ=255)
                                                                                                                                                    cATCCGCAATCAACGCCTGCTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                               <  1:756930/289‑1 (MQ=255)
                                                                                                                                                         gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                                                                          <  1:185855/289‑1 (MQ=255)
                                                                                                                                                              cAACGACTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCATAAAGCGCATTAGCCTGAATTAGCGTGATTTTGTAGGTCGGATAAGG‑CGGTT‑‑A‑‑CGCTGCATCCGACATCAATTCCTGATGCGACGCTGGACTCGTCGTAGTAGGCCTATCTTAACCGTTAGTTAATTTTTCAAACTCTTCACTCCCGGTCTCAACCCTATCATTACAACCAAGCTTCAACCAGTTCACCTCCTTTTG‑CTTGTTATTCCGTATCAAACTCTCTTCATCGTTCACGCACCTCAtta                                                                                     >  2:608751/1‑288 (MQ=12)
                                                                                                                                                              aaacggatgattgtaCGATTAACGCGTATTATCAGGCATACGCCAGACAGTGCAATAGGCTGATTTAGCGTGATTTTGTAGGTAGGATAAGG‑CGTTT‑‑ACGC‑C‑GCAGCCGACATCAATGCCTGATGCTACGCTTGCCGCGGCTTATCATGCCTATCTTAACCTTTTGTTAATTTTTCAAACTCTTCACACACTGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTCAAGAACATCATTc                                                                                     <  2:727553/275‑1 (MQ=255)
                                                                                                                                                                aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTccc                                                                                   <  1:474320/289‑1 (MQ=255)
                                                                                                                                                                 cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTccc                                                                                   >  1:613138/1‑288 (MQ=255)
                                                                                                                                                                       aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTCTCAAACCCTTCCATACAGCTCAGGTTCAACCCGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAACCCTTCTCCACCGTTTAATAACCTCAATCCCGCCCCGt                                                                            >  2:17342/1‑289 (MQ=21)
                                                                                                                                                                       aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                            >  1:407915/1‑289 (MQ=255)
                                                                                                                                                                                  aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCTCCCAGTACTCGGAATa                                                                  >  1:356193/1‑288 (MQ=255)
                                                                                                                                                                                   cctgttCTTTTCAGGCCTACGCCAGAAAGAGCAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc                                                                <  2:583762/284‑1 (MQ=255)
                                                                                                                                                                                        tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACGTCAATGCCTGAGGCGACGCTTGCCGCGGCTTCTCAGGCCTATCTTAACCGTCTGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAACCCTTCCATCAAGCTCCGGTTGACCCAACAACGCGCCTTTTGCCTTGTT‑TTCACAATACATCCTCTCTCACCTTTTAAGAACATCATTCCACTCCCGTACTCGCAATCACCGGtt                                                           >  2:286121/1‑289 (MQ=12)
                                                                                                                                                                                         cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                         <  2:399697/163‑1 (MQ=255)
                                                                                                                                                                                         cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                                                                         >  1:399697/1‑163 (MQ=255)
                                                                                                                                                                                         cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTc                                                          >  1:77851/1‑289 (MQ=255)
                                                                                                                                                                                             tccggCCTACGCCAGACAGCGCAATAGCCTTATTTCGCGTGATTTTGTATGTCGTATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGGATAACCGGTTCGGGa                                                      <  1:628648/286‑1 (MQ=255)
                                                                                                                                                                                                 gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                                                                          >  1:84069/1‑249 (MQ=255)
                                                                                                                                                                                                 gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcat                                                                                          <  2:84069/249‑1 (MQ=255)
                                                                                                                                                                                                      cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGACATTGCaga                                             >  1:636210/1‑289 (MQ=255)
                                                                                                                                                                                                      cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCaga                                             >  1:352994/1‑289 (MQ=255)
                                                                                                                                                                                                      cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGCTCACCCCTTCTTCACCGTTTAAGAACATCATTACCGCCCCTTACTCGGACTCACAGGACCGGGCAATTTCaca                                             >  2:565726/1‑287 (MQ=25)
                                                                                                                                                                                                       gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ATGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag                                            <  1:95455/289‑1 (MQ=255)
                                                                                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGTATAACCGGTTCGGGAAATTGCAGAGCTGc                                        >  1:270860/1‑289 (MQ=255)
                                                                                                                                                                                                           gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGACTAACCGGTTCGGGAAATTGCAGAGCTGc                                        >  1:166669/1‑289 (MQ=255)
                                                                                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑TCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCCCGATACAACCTTCTTCACCGTTTACGAACATCATTCCCGCCCATTTCCCGGAATAACCGGTTCCGACAATTTCAGACGTGCttttc                                   >  2:431227/1‑288 (MQ=37)
                                                                                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCCTTCCCGCCCAGTACTCGGAATCACCGGTTCGGGACATTGCAGAGCTGCCTTTg                                   >  2:399205/1‑289 (MQ=255)
                                                                                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGGTTg                                   >  1:715864/1‑289 (MQ=255)
                                                                                                                                                                                                                cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGAGAAAACCTTCTTCACAGTTTAAGAACATCATTCCCGCCCACTACTCGGACTAACCGGTTCGGGAACTTGCCGAGCTGAGtttt                                   >  2:165254/1‑288 (MQ=37)
                                                                                                                                                                                                                    aTAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGT‑CTGTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAAt                               <  1:649831/289‑1 (MQ=255)
                                                                                                                                                                                                                     tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATa                              <  1:316560/289‑1 (MQ=255)
                                                                                                                                                                                                                     gaGCCTGGTTTCGCTTGCTTTTGTGGGTCGGATAAGGCCGTTC‑‑AC‑C‑C‑GCATCCGACTGCAATGCCGGATGCGATGCTTGCCTCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATa                              <  2:724113/288‑1 (MQ=255)
                                                                                                                                                                                                                       gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATAcc                            <  2:352994/289‑1 (MQ=255)
                                                                                                                                                                                                                          tGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                         >  1:460103/1‑289 (MQ=255)
                                                                                                                                                                                                                          tGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                         >  1:454416/1‑289 (MQ=255)
                                                                                                                                                                                                                          tGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                         >  1:165346/1‑289 (MQ=255)
                                                                                                                                                                                                                                  cGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTACCGCCCAGTACTCGGAATAACCGGTTCGGGCACTTGCAGAGATGCGTTTGACATACCAGGCTGACTTg                 >  1:681515/1‑289 (MQ=38)
                                                                                                                                                                                                                                      aTTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTAGGGAAATTGAAGAGCTGCGTTTGAAATACCAGTTTGCCTTGTCa              >  1:406121/1‑288 (MQ=255)
                                                                                                                                                                                                                                      aTTTTGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTAATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAACTTGCAGAGCTGCGTTTGAAATCCCAGGTTCCCTTGTCa              >  2:210797/1‑288 (MQ=255)
                                                                                                                                                                                                                                         ttGTAGGTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgt          <  1:431227/289‑1 (MQ=255)
                                                                                                                                                                                                                                               gTCGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATCCCAGGTTGCCTTGTCATCGTCGGCCt    >  1:142126/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 cGGATAAGG‑CGTTT‑‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATCAAACCTTCTTAACCGTTTAAGAACATCATTCACGCCTATTACTCGGATTAACCGGTTCGGGCAATTCCAGAGCTGCGTTTGCAATACCAGTTTGCCTTGTCATTGTCGGCCTTa  >  2:214742/1‑289 (MQ=38)
                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                                   
CGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGG‑CGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG‑CTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTA  >  NC_000913/4296125‑4296652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑