Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,075,154 Δ2 bp coding (486‑487/786 nt) yihW → putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,075,1540G.93.3% 236.3 / 8.1 60coding (486/786 nt)yihWputative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base . (31/25);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,075,1550T.94.9% 227.0 / 6.4 60coding (487/786 nt)yihWputative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (31/25);  minor base A (3/0);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGCGTCCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAAT  >  NC_000913/4074881‑4075423
                                                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                            
gTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCCTGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACCAACCTCGCTTTGAAGTGATGGTGCCCGGCGGTACGGTGCGCTTCCATACTCGCGGGATCCTTCGCCCTTCCGCG‑‑GACCTTCGTGGCTGAttt                                                                                                                                                                                                                                                              >  2:481454/1‑289 (MQ=255)
gTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCGCATAATAGCGGGATCATTGGCCCTTCA‑‑AGCGCCATGTGTGGCTGAttt                                                                                                                                                                                                                                                              >  1:456051/1‑289 (MQ=255)
            tGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGCTTa                                                                                                                                                                                                                                                  >  1:90528/1‑289 (MQ=255)
                    cGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCCTTGGCCCTTCAGCGGC‑‑CCTTTGGGGCTGCTTTTCGTGCTTATTACCTgttct                                                                                                                                                                                                                                          >  2:375956/1‑285 (MQ=255)
                    cGCAAACCGAGG‑AAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAAc                                                                                                                                                                                                                                         <  1:15691/289‑1 (MQ=255)
                         aCCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGGGGCTGATTTTCGTGCTGATTATCGGGTAACAAGc                                                                                                                                                                                                                                     >  1:436136/1‑289 (MQ=255)
                           cGAGGAAAAAAAAGCGATTGCCGAAGCTGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGt                                                                                                                                                                                                                                   <  1:375956/289‑1 (MQ=255)
                                                                  tatTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCTTGCTGATTATCTGGTAACCAGCGTTGGGGCGATTGAGATCGATGGCGCGGTGATGGTGGTTGa                                                                                                                                                                                            >  2:446682/1‑289 (MQ=255)
                                                                        tGATGGTTCAACAATATTTATCACCATTGGTTCGACAGTTGAGCATGTTGCCCTGGCGTTACTTAACCATAATCATTTGCGGATAGTCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCAGTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTaaa                                                                                                                                                                                      <  1:460145/289‑1 (MQ=255)
                                                                        tGATGGTCCAACAATATTTTGAACCATTGGTACGACTGTTGGGCATGTTGCCCGTGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCGTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTaaa                                                                                                                                                                                      <  1:506579/289‑1 (MQ=255)
                                                                         gATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGATCATGTTGCCGGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAAc                                                                                                                                                                                     <  1:614884/289‑1 (MQ=255)
                                                                           tgtttCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGa                                                                                                                                                                                   <  1:481454/286‑1 (MQ=255)
                                                                           tGGTTCAACAATATTTATCACCATTGTGACGACTGTTGAGCATGTTGACCGGGCGTTACTTAACCATAATCTTTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGa                                                                                                                                                                                   <  1:234493/289‑1 (MQ=255)
                                                                            ggTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGGGGCTCATATTCTTTACCACAAACCGCGCTTTGAAGTGAAGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCCTCGGC‑‑ACTTTGTGGCGGTTTTTCGTGCTGAATATCTTGTCACAAGCGTGGGTGCGGTTGCAGGCCGTGGCGCGTTTGTGGCGTTTGATGTGAACgtg                                                                                                                                                                                  >  2:484013/1‑287 (MQ=255)
                                                                                 aacaaTATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTa                                                                                                                                                                              >  1:781588/1‑288 (MQ=255)
                                                                                  acaaTATTTATCACCATTGGTACACTTTTTGAGCATGTTGCCCTGGCGTTACTTAACCATAATCATTTGCGGAGAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCAGTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAAc                                                                                                                                                                            <  2:397402/289‑1 (MQ=255)
                                                                                      tatTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGTTAACACGCGTTGGGGCGATTGAGGGCGTTGGGGCTGTGATGGAGTTTGATGGATACGAAGCTagagtga                                                                                                                                                                        >  2:491915/1‑283 (MQ=255)
                                                                                        tttATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCG‑‑GACCGTTGGGGCGGATTGTCGGGCTGATTATCTGGTAACAAGCGTTGGGGCGATGGAGAGCGATGGCGCGTCGAGGGAGTTTGATGGAAAAGAAGCTAAAgtggtg                                                                                                                                                                      >  1:359434/1‑289 (MQ=255)
                                                                                             cACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATTTGGTAACAAGCGTTGGGGCGGTTGAGTGGGATGGCGCGTTGAGGGAGTTTGATGGAAGCGACGGTCACGGGGGGGAAAc                                                                                                                                                                 >  2:756192/1‑289 (MQ=255)
                                                                                                 aTTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGGTCTGGTGCGGGTTGATGGGGTTTGGTGTAAACGAGTGTAAAGGGGTGAAAACgatg                                                                                                                                                             >  2:33882/1‑289 (MQ=255)
                                                                                                  ttGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGGGAGTGAGAGCGATGGCGCGTTGATGGAGGTTGATGTAAACGAAGGTAACGTGGTGAAAACgagga                                                                                                                                                            >  1:433821/1‑289 (MQ=255)
                                                                                                       gctaCTTTTGCGCATGTTCCCCGGGCTTTCCTTCACCCGTATCATTTGCGGCTAATGCCCAACAGCCTGCGTGTTGCTCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCTTAATAGCGGGATCCTTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGgcg                                                                                                                                                       <  2:359434/286‑1 (MQ=255)
                                                                                                          aCTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGCTCATTGGCCCTTCAGCG‑‑GACCTTCGTGGCTGATTTTCGTGCTGATTGTCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGGGTGGATGGAGTTTGATGTAAACGAAGCTAACGAGGTGAAAAAGATGAGGGCGAAc                                                                                                                                                    >  1:581039/1‑289 (MQ=255)
                                                                                                            tGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCAcgc                                                                                                                                                  <  1:117766/289‑1 (MQ=255)
                                                                                                              ttGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCCTATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCGGGTAACAAGCGTTGGGGCGATTGAGAGGGAGGGCGCGTGTGTGGTGTTGGATGTAAACGACGCTAAAGGGGTACAAACGTTTATgggggtagcga                                                                                                                                                >  2:599728/1‑280 (MQ=255)
                                                                                                              ttGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGACGCTAACGTGGTGAAAACGATGATGGCGCACGCga                                                                                                                                                >  1:806634/1‑289 (MQ=255)
                                                                                                               tGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCgag                                                                                                                                               <  1:33882/289‑1 (MQ=255)
                                                                                                                      gTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAt                                                                                                                                                                                                     <  2:636114/228‑1 (MQ=255)
                                                                                                                      gTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAt                                                                                                                                                                                                     >  1:636114/1‑228 (MQ=255)
                                                                                                                                gcgtACTTAACCATAATCATTTTCGGTTAATCACCTACAGCCTGCTTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGTTAGTGCCCGGCGGTACGTTACGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTct                                                                                                                              <  1:420740/286‑2 (MQ=255)
                                                                                                                                     cTTAACCATAATCATTATCGGTCAAGCACCAACAGCCTGCGTGTGGCGCACATTCTTTACCCCAACCCACGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGAt                                                                                                                         <  2:436136/289‑1 (MQ=255)
                                                                                                                                             tAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGAGCATATTCGTTACCACAAACCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCCTACTAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTCGCTGATTTTCGTGCTGATAATCGTGTAACAAGAGTGTGGGCGAGTGCGAGAGATGGCGGGTGCATGGCGTTTGATGTAAAGGCAGAGCAAGTGGTAAAAACGGTGATGGCGCACACGGAAACTATTGTGCTTGTAGCGAATCACACtga                                                                                                                 >  2:363769/1‑287 (MQ=255)
                                                                                                                                               aTCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTTGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGCCGCAGGCGAGAAATATTCTGCTGGTTCCCGATCACACTAAGt                                                                                                               >  1:579138/1‑289 (MQ=255)
                                                                                                                                                   tttGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCa                                                                                                           <  1:491915/289‑1 (MQ=255)
                                                                                                                                                                        ccTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGGATCATGCTTCGGCGGCGGTTGAAAt                                                                                      >  1:786617/1‑289 (MQ=255)
                                                                                                                                                                            ggtttgGGGCATATTCGTTACCACAACCCGGGGTTTGAAGTGATGGTGCCCGGGGGTACGTTTCGCGCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCGTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTgg                                                                                   <  2:781588/284‑1 (MQ=255)
                                                                                                                                                                            cGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCg                                                                                                                              >  2:206971/1‑245 (MQ=255)
                                                                                                                                                                            cGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCg                                                                                                                              <  1:206971/245‑1 (MQ=255)
                                                                                                                                                                            cGTGTGGCGCATATGCTTTACCCCAACCCGCGCTTTGTAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTgg                                                                                   <  1:484013/288‑1 (MQ=255)
                                                                                                                                                                                gggcggcatttCTTTACCACCTCCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATACTTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACg                                                                              <  2:786617/280‑1 (MQ=255)
                                                                                                                                                                                 ggCGCATATTCTTTACCGCAGACCGGGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATAATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACg                                                                              <  1:7916/288‑1 (MQ=255)
                                                                                                                                                                                      atatTCTTTACCACAACCCGCGGTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGca                                                                         <  1:599728/288‑1 (MQ=255)
                                                                                                                                                                                      atatTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGca                                                                         <  1:756192/288‑1 (MQ=255)
                                                                                                                                                                                            tttACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCt                                                                                                                                                                                                                                                                   >  1:202766/1‑96 (MQ=255)
                                                                                                                                                                                            tttACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCt                                                                                                                                                                                                                                                                   <  2:202766/96‑1 (MQ=255)
                                                                                                                                                                                             ttACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAATCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGCCGGTTGAAATTGGTAACGTGGCACAGGTc                                                                   >  1:667578/1‑287 (MQ=255)
                                                                                                                                                                                                    aaCCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAAGATTCTGCTGGTCGCCGGTCACACTAAGTATAATGCTGCGGAGGCGGTTGAAATTGGTAACTGGGGACCGGGCAATgcgc                                                            >  1:743635/1‑287 (MQ=255)
                                                                                                                                                                                                      cccGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTAt                                                                                                                                                                                                                                                 <  2:478520/104‑1 (MQ=255)
                                                                                                                                                                                                      cccGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTAt                                                                                                                                                                                                                                                 >  1:478520/1‑104 (MQ=255)
                                                                                                                                                                                                      cccGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAACAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGGGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGGGGTGAAAACGATGATGGCGAACGCGAGAAAACTACTGATGGTCGCCGATCATACAAAGTATCATGCTGCGCCGGGGGGTGAAATTGGTCACGTGTAACAAGTAACTgcgcctca                                                        >  2:712351/1‑285 (MQ=255)
                                                                                                                                                                                                          cgcTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTAcc                                                    >  1:395295/1‑289 (MQ=255)
                                                                                                                                                                                                                           gCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGCCACTGCGCTCTTTACCGCCTAGCTgccgccag                                    >  1:31488/1‑286 (MQ=255)
                                                                                                                                                                                                                            cccGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACg                                                                              <  1:658345/245‑1 (MQ=255)
                                                                                                                                                                                                                            cccGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACg                                                                              >  2:658345/1‑245 (MQ=255)
                                                                                                                                                                                                                                 cggTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCt                             >  1:365828/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTAACGACGAGCTGCCGACCGCTggcgtaaa                          >  1:563941/1‑282 (MQ=255)
                                                                                                                                                                                                                                                tcATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGCGCTGCCGCCCGCTGCGCTAAAATCACGCTTACa              >  1:509036/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACCCTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCaa    >  1:334523/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTTGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTgccgcc                                      >  1:257781/1‑255 (MQ=255)
                                                                                                                                                                                                                                                            gATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAACTTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACTAGCTGCCGACAGCTGCGCTAACATCACGCCTACCAGACAGCCaact  >  2:571988/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                            
GTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGCGTCCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAAT  >  NC_000913/4074881‑4075423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑