Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,154	Δ2 bp	coding (486‑487/786 nt)	yihW →	putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,154	0	G	.	100.0%	260.3 / NA	60	coding (486/786 nt)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (36/24);  total (36/24)
*	NC_000913	4,075,155	0	T	.	100.0%	260.3 / NA	60	coding (487/786 nt)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (36/24);  total (36/24)

CGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGCGTCCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAAT  >  NC_000913/4074880‑4075423                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                             cGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGTTTCCCGGCGGTACGTTGCGCTGTCCTAATAGCGGGCTCTTTTGACCTTCCGCGCC‑‑CCTTGGTGGCCGAtt                                                                                                                                                                                                                                                               >  2:343933/1‑289 (MQ=255)           gcGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGa                                                                                                                                                                                                                                                     >  1:395022/1‑289 (MQ=255)                aGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTAt                                                                                                                                                                                                                                                 <  1:432040/288‑1 (MQ=255)                      cGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCCGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTa                                                                                                                                                                                                                                           >  1:242099/1‑288 (MQ=255)                                  aaaaacaaTCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTggg                                                                                                                                                                                                                               <  1:47916/288‑1 (MQ=255)                                                           ggCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAt                                                                                                                                                                                                     >  1:118539/1‑289 (MQ=255)                                                                aCTATATTCTGGATGGGTCAACAAGATTTAGAACCATTGGTACGAATGTTTAGCAGTTTGCCCGGGCGTTACTGAACCATAAGCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCAGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGt                                                                                                                                                                                                <  2:108059/289‑1 (MQ=255)                                                                 cTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGtt                                                                                                                                                                                               <  2:85671/289‑1 (MQ=255)                                                                    tatTCCTGATGGTTCAACAATATTTATCACCAGTGATACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATACTTTACGGATAATCACCACCAGCCTGCGTGTGGCGCATATTCTTTACCACCACCCGCGCTTTGCAGTGATGGTGCCCGGCAGTACGTTGCGCTCTCATACTATCGGAATCATTTGCCCTTCAGCTGC‑‑CCGTTGTGGGTTCTTTTCGTTCTGGTTCTCTGGTACCAAGCTTTGGGGTCATTGGGTGCTGTGGCGCTTGTATGGAGttggg                                                                                                                                                                                            >  2:678317/1‑286 (MQ=255)                                                                       tCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGGGATGGGGCGTTGATGGAGTTGGATGt                                                                                                                                                                                         >  1:189332/1‑289 (MQ=255)                                                                         cTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTaa                                                                                                                                                                                       >  1:151200/1‑289 (MQ=255)                                                                          tGAGGGGTCAACAATATTTATCACCCCTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAAGCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCGTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTaaa                                                                                                                                                                                      <  2:100401/289‑1 (MQ=255)                                                                            aTGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACg                                                                                                                                                                                    >  1:325757/1‑289 (MQ=255)                                                                            aTGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATATGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAGGGAGTTTGATGTAAACg                                                                                                                                                                                    >  1:680346/1‑289 (MQ=255)                                                                                   aacaaTATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATAGTCTTTACCACAACCAGCGCTTTGCAGTGATGGTGACCGGCGGTCTGTTGCGCTCTCATAAGATCGGGATAATTGGCACTTCAGCGGC‑‑CATTTGGGGCTGAGTTTAGTTCTGATCCTCCGGTAACAAGTGCTAGGGGGGTTGCGAGCGGTGGCGCGGTGATGTAGGTTGATATacactcagatac                                                                                                                                                                             >  2:661837/1‑281 (MQ=255)                                                                                        tatTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTTGGGTAAACGAGGCTAACgtgg                                                                                                                                                                        >  2:204595/1‑289 (MQ=255)                                                                                          tttATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACgtggtg                                                                                                                                                                      <  2:118539/289‑1 (MQ=255)                                                                                            tgttaCCATTGGTAAGACGTTTAGGCATGTTGGCAGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGaa                                                                                                                                                                    <  2:404620/285‑1 (MQ=255)                                                                                                aCCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAGGCTACCGTGGTGAAAACg                                                                                                                                                                >  1:144549/1‑289 (MQ=255)                                                                                                  cATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTTCCCGGCGGTACGTTGCGCTCTCATAGTAGCGGGATCATTGGCCCTTCGGCGGC‑‑CCTTTGTGGCTGATTTTCTTGCTGATTTTCTTGTAACAAGCGTTGGGGCCATTTAGATCGCTGGCACGTTTTGGTAGTGTGATTTAAACGAACTTCAAGTGGTGACAATgag                                                                                                                                                              >  2:567381/1‑288 (MQ=255)                                                                                                    ttGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTgggg                                                                                                                                                                                                                              <  1:462716/223‑1 (MQ=255)                                                                                                    ttGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTgggg                                                                                                                                                                                                                              >  2:462716/1‑223 (MQ=255)                                                                                                    ttGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGGGAAAACgatga                                                                                                                                                            >  1:116156/1‑289 (MQ=255)                                                                                                         aCGACTGTTGAGCATGTTGCCCGGGCTTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGgcg                                                                                                                                                       <  1:16852/289‑1 (MQ=255)                                                                                                          cGACGGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGgcgc                                                                                                                                                      <  1:574378/289‑1 (MQ=255)                                                                                                           taCTGTTGAGCAGTTTGCCCGTGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGGGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCa                                                                                                                                                     <  2:261936/288‑1 (MQ=255)                                                                                                            aCTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTTGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGTTTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAGGGAGTTTGATGTACACGAAGCTAACGTGGTGAAAACAATGATGGCGCAc                                                                                                                                                    >  1:401779/1‑289 (MQ=255)                                                                                                              tGTTGAGCATGTTGCCGGGGCGTGACTTAACCATAATCATTTGCGGATAATGACCAACAGCCTGCGTGTGGCGAATAGTCTTTACCACAACCCGCGATTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCAcgc                                                                                                                                                  <  1:139725/289‑1 (MQ=255)                                                                                                                  gAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCgaga                                                                                                                                              >  1:364566/1‑289 (MQ=255)                                                                                                                     cATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGACCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCCTTGTGGCGGATTTTCGTGCTGCTTATCTGGTAACAAGCGTTGGGGCGATTGAGAGAGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGATAACGTGGTGAAAACGAGGATGGCGCAAGCGAGAAat                                                                                                                                           >  1:519321/1‑289 (MQ=255)                                                                                                                                       cTTAACTATAATCATTTGCTGTGAACCACCAACAGCCTGCGTGTGGCGCATATTCGTTACCACAACCCGCGCTGTGAAGGGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGAt                                                                                                                         <  2:369925/289‑1 (MQ=255)                                                                                                                                        ttAACCATCATCATTTGCGGATGATCCCCAACAGCCTGCGTGTGGCGCATATTCTTTACCACTACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATc                                                                                                                        <  1:296876/289‑1 (MQ=255)                                                                                                                                           aCCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCTCGTTGATGGATGTTGATGTACAAGAAGATAACGTGGTGACAACGATGATGGCGCCCGCGAGAAATAGTCTGATGGTCGCCGATcca                                                                                                                      >  1:338855/1‑286 (MQ=255)                                                                                                                                             cATAATCGTTTGCGGGTAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTGTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACt                                                                                                                   <  1:411339/289‑1 (MQ=255)                                                                                                                                             cATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACt                                                                                                                   <  1:654833/289‑1 (MQ=255)                                                                                                                                                 aTCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTCTCTGGTAACAAGCGTTGGGGCGATTTCGAGCGATGCGGCGTTGATTGAGTTTGATGGAAGCGACGCTAACGTGGTGAAAACGATGTGTGCGTAACCGAGAAACATGAGTCTGCTCGCCGATCACACTaatg                                                                                                               >  2:352906/1‑287 (MQ=255)                                                                                                                                                              aaTCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAt                                                                                                                                                                                                     >  1:358242/1‑190 (MQ=255)                                                                                                                                                              aaTCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGAt                                                                                                                                                                                                     <  2:358242/190‑1 (MQ=255)                                                                                                                                                                          ccTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGATTTTGATGTAAACGAAGCTAAAGTGGTGAAAACGATGATGGCGCACGAGAGAAATATTCTGCTGGTCGCAGATCACACTCAGTCTCATGCTTCGGCGGGGTGTGAAAt                                                                                      >  1:373024/1‑289 (MQ=255)                                                                                                                                                                          ccTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGAGAAAACGATGATGGCGAACGAGAGAAATATTCTGCTGGTCGACGAGCACACTAAGTATCATGCTTCGGCGGCGGTTGAAAt                                                                                      >  1:19854/1‑289 (MQ=255)                                                                                                                                                                              cGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGACGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTgg                                                                                   <  1:204595/288‑1 (MQ=255)                                                                                                                                                                                 gtgGCGCATATTCTTTACCACAACCCGCTAGTTGAAGTGAGGGTGCCCGGCGGTACGGTGCGCTCTCATAAGAGCGGGATCATTGGCCCTTCATAGGC‑‑CCGTTGTGGCTGATTTTCGGGCTGAGTAGCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGAGGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATGGGTaa                                                                                <  1:389422/288‑1 (MQ=255)                                                                                                                                                                                    gcgcATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTTAAATTGGTAACGTg                                                                            >  1:308543/1‑289 (MQ=255)                                                                                                                                                                                        atatTCTGTACCACAACCCGCGCTTGGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGca                                                                         <  1:131235/288‑1 (MQ=255)                                                                                                                                                                                        agatgttttACCATAACCCGCGCTTTGAAGTTGTGGGGCCCGGCGTGACGTTGCGCTCTAATAATAGCGGGGTCATTGGCCCTTCAGCGGC‑‑CTTTTGTGGCTGATTTGCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGcac                                                                        <  2:63169/283‑1 (MQ=255)                                                                                                                                                                                            tCTGTACCACAACACGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGAGCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGt                                                                    <  1:7379/289‑1 (MQ=255)                                                                                                                                                                                                   cgcaACCCGGGCTTTGAAGTGATGGGGGCCGGCGGTACGTTGGGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTgcg                                                             <  2:151200/287‑1 (MQ=255)                                                                                                                                                                                                      aaCCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTAATGGAGTTTGATGTCAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAACTTGGTAACGTGGCAAAGGTCACTgctc                                                            >  1:313791/1‑285 (MQ=255)                                                                                                                                                                                                             gcTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGGGAAAACGTTGATGGCGCACGCGAGAAATATTCTGCTTGTCGCCGATCACACTCAGTAACATGCATCGGCGGGGGTTTAAATTGGTACACTGGAACAGGTAACTGGCATCTTTAccc                                                   >  2:579597/1‑288 (MQ=255)                                                                                                                                                                                                                         tGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTGTACCGACGAGCTgccg                                        >  1:114750/1‑288 (MQ=255)                                                                                                                                                                                                                                           tGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGGTGGTCGCAGATCACACTAAGGATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGGAACTGCGCTCTTTACCGCCGACCTGCCGCCCGCTGCCCTCAACTCAc                     >  2:387038/1‑289 (MQ=255)                                                                                                                                                                                                                                           tGCGCTCTCATAATAGCGGGATCATGGGCCCTTCAGCTGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCAc                     <  1:300862/289‑1 (MQ=255)                                                                                                                                                                                                                                             cgcTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACCCTAAGTCTCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCAc                     >  1:320629/1‑287 (MQ=255)                                                                                                                                                                                                                                             cgcTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCAc                     >  1:68178/1‑287 (MQ=255)                                                                                                                                                                                                                                                            gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATCTTCTGCTGGTCGCCGATCAACATAAGTATCATGCCTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGACCTGGCGCCCGCTGCGCGAAAATCCCGCTTACGAGAACGCCaa    >  2:616689/1‑289 (MQ=255)                                                                                                                                                                                                                                                            gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCTCCCTTTACCCACGACCTGCCGCCCGCTGCGCTAAAATCCCGCTTACAAAACATCCaa    >  2:594606/1‑289 (MQ=255)                                                                                                                                                                                                                                                            gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCaa    >  1:154587/1‑289 (MQ=255)                                                                                                                                                                                                                                                            gggATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGAACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCCTACAAGACAGCCaa    >  1:266727/1‑289 (MQ=255)                                                                                                                                                                                                                                                              gATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAAt  >  1:578582/1‑289 (MQ=255)                                                                                                                                                                                                                                                              gATCATTGGCCCTTCAGCGGC‑‑CCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGACCCGGTCCATTAGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCCCGCCTACAAGACAGCCAAAt  >  2:664837/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                             CGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAGCATGTTGCCCGGGCGTTACTTAACCATAATCATTTGCGGATAATCACCAACAGCCTGCGTGTGGCGCATATTCTTTACCACAACCCGCGCTTTGAAGTGATGGTGCCCGGCGGTACGTTGCGCTCTCATAATAGCGGGATCATTGGCCCTTCAGCGGCGTCCTTTGTGGCTGATTTTCGTGCTGATTATCTGGTAACAAGCGTTGGGGCGATTGAGAGCGATGGCGCGTTGATGGAGTTTGATGTAAACGAAGCTAACGTGGTGAAAACGATGATGGCGCACGCGAGAAATATTCTGCTGGTCGCCGATCACACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAAT  >  NC_000913/4074880‑4075423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑