Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,070,814	G→T	R315S (CGT→AGT)	yihS ←	sulphoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,070,814	0	G	T	100.0%	233.9 / NA	63	R315S (CGT→AGT)	yihS	sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/26);  total (37/26)

AGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCAGCGGATTCCACTGGGTA  >  NC_000913/4070534‑4071094                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         cgcatccccGGTGCAACCCCTGGATCCAGGCACGTCTCTAATGGGATTAGCGGATTCACCAGTCAATGCGGCATGGGATACCTCTCCTGTTGGCCGCCCCAGACGTTGGTGGTGACCTGATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCTGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGtt                                                                                                                                                                                                                                                                                  <  2:190646/283‑1 (MQ=255)   cagACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCCTGTTTGATACCAGGTTTCCTACTGGCTATCGCCCGTGACGGTGTAGAGCGCGTAGGCCGTACCCAGTGCTTCCACGCTAGCACAACTTAAACGTTc                                                                                                                                                                                                                                                                                 >  2:358133/1‑288 (MQ=255)    agACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTcg                                                                                                                                                                                                                                                                                <  1:511664/288‑1 (MQ=255)      acacGCTGCAACCGCTGGAGACATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGACCCAGAATTAGGTGGTGACCTGCTTGGACGCATCCTGCTACTGCCACCAGGAACCATTTTCAGAGTCCATCAGGTACTTAATGCAGTACTCCCAACATGTTTGAACCCAGGCTTGATACTGGCAATCCCCAGTGCCGGTGTAGAGCGCGTAGACCGTACCCATTGCTTCGAGAATCTGCCACCTTACAGGttagggt                                                                                                                                                                                                                                                                             >  2:247108/4‑284 (MQ=255)      aCCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCgg                                                                                                                                                                                                                                                                             >  1:251490/1‑289 (MQ=255)                              gCCGGGCGTTAACGGGGTACGCGGGATCACCAGGAAATGCAGCAGGTGATAAATTTCCTTTGTTCCGGCCCAGACTTTGGGGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCa                                                                                                                                                                                                                                                     <  2:284960/289‑1 (MQ=255)                               ccgttcgcTAACGGGCAACGCGGGTGCCCCGGGCAATGCAGCAGCTGATAAATATCCTGTTTGCCTTCCCCGACTTTGTTGGTGACCGTATTGTCCGCATCCAGCTCCTTCCACCAGGAACCATTTTCATAGTCCCTCAGTTACTTAATGCAGTACTCCCACCATGTTTGATACCAGATTGCATACTGGCTATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCaa                                                                                                                                                                                                                                                    <  2:654442/284‑1 (MQ=255)                               ccGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCa                                                                                                                                                                                                                                                     <  1:389409/288‑1 (MQ=255)                                                                cAATGCAGCCGGTGATAAATATCCTGGTTGCCGTCCCAGACTTTTGTTGTGACCTTATTGTCCGCAGCCAGCTCCTTCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCggg                                                                                                                                                                                                                     <  2:413440/287‑1 (MQ=255)                                                                             gATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATCGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCATCGGTATAAACATTCCCGTCCGCGCCCTCGGGTGCCCAGGCATcgc                                                                                                                                                                                                       >  2:206906/1‑288 (MQ=255)                                                                                  atatCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACgg                                                                                                                                                                                                 >  2:262172/1‑289 (MQ=255)                                                                                  atatCCCGTGTGCCGGCCCAGATCTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCGGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCAGGTTTGATACCAGGTGTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACgg                                                                                                                                                                                                 <  2:423308/289‑1 (MQ=255)                                                                                     tCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTg                                                                                                                                                                                               >  1:18822/1‑288 (MQ=255)                                                                                                         gttGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAAGAACCATGTTCATAGTCCATCAGGTACTTAATTCAGTACTCCCCCCATGTTTGAGACCAGGTTTCATACTGGCGATCGCCAGTGACTGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCTGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCa                                                                                                                                                                          <  1:286929/288‑1 (MQ=255)                                                                                                             gtggtgACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCAtcg                                                                                                                                                                       >  1:153714/1‑287 (MQ=255)                                                                                                                   aCCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTagca                                                                                                                                                                >  1:255107/1‑289 (MQ=255)                                                                                                                     cTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCCTCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTagcag                                                                                                                                                               >  1:404980/1‑288 (MQ=255)                                                                                                                              cGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCtggt                                                                                                                                                     >  1:398223/1‑289 (MQ=255)                                                                                                                              cGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGAGTACGGTGGCGTTAAACAGACCTTTGGCATCTTTTAGCAGCCATGCtggc                                                                                                                                                     >  1:179291/1‑288 (MQ=255)                                                                                                                               gCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTGGGCATCTTCTAGCAGCCATGCtggtt                                                                                                                                                    >  1:272148/1‑288 (MQ=255)                                                                                                                                                 caGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAAACCCGTCCGCGCCATCGGGTGCCCAGGCATCGCCCACGGTGGCGTTACACAGACCTTTGGCATCTTGTAGCAGCCATGACGCGGGGTGTTCGCAACggtcc                                                                                                                                   >  2:94634/1‑285 (MQ=255)                                                                                                                                                     aaCCATTTTCATAGTCCATCAGGTTCTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCg                                                                                                                                                                                            <  1:328098/227‑1 (MQ=255)                                                                                                                                                       ccATTTTCATAGTCCATCAGGGACTTAAGGCAGTACTCCCACCATGTTTGATACCAGGTTTCATCCTGGCGATCGCCAGTGACGGTTTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAggg                                                                                                                            <  1:358133/289‑1 (MQ=255)                                                                                                                                                                  gTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAtg                                                                                                                 >  1:32838/1‑289 (MQ=255)                                                                                                                                                                   tCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCCTCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGCCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCACCGGGCTTCCAGGGCAGCATgtagtg                                                                                                                >  1:304232/1‑284 (MQ=255)                                                                                                                                                                    ccttCAGGTCCTTAATGCAGTAACCCCACCATGTTTGTAACCACGTTTCATACTGGCGATCGACAGTGACGGGGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAtgtg                                                                                                               <  2:548530/286‑1 (MQ=255)                                                                                                                                                                    ccATCCGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGCTGCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTACAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAtgtg                                                                                                               <  1:423946/289‑1 (MQ=255)                                                                                                                                                                     cATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAtttg                                                                                                               >  1:151670/1‑288 (MQ=255)                                                                                                                                                                     cATCAGGTACTTAATGACTGACTCACACCATGGTTGCTACCAGGTTTCAGACTGGCGATGGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAtgtg                                                                                                               <  2:143887/288‑1 (MQ=255)                                                                                                                                                                             aCTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCCACGGTATAAACAATCCCGTCCGCGCAATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGCCATCTTCTAGCAGCCATGCGGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCAGGGATCGTCCGCATTaa                                                                                                      >  2:234864/1‑289 (MQ=255)                                                                                                                                                                             aCTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTTCAGCATTaa                                                                                                      >  1:654271/1‑289 (MQ=255)                                                                                                                                                                             aCTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaa                                                                                                      >  1:143887/1‑289 (MQ=255)                                                                                                                                                                              cTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaaa                                                                                                     <  1:206906/289‑1 (MQ=255)                                                                                                                                                                                   tGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCAATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGc                                                                                                 >  1:602724/1‑288 (MQ=255)                                                                                                                                                                                        tACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCggg                                                                                                                                                                                                                     >  1:507914/1‑167 (MQ=255)                                                                                                                                                                                        tACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCggg                                                                                                                                                                                                                     <  2:507914/167‑1 (MQ=255)                                                                                                                                                                                             ccaccaTGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGAt                                                                                       >  1:495647/1‑288 (MQ=255)                                                                                                                                                                                               accaTGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCa                                                                                    >  1:436374/1‑289 (MQ=255)                                                                                                                                                                                               accaTGTTTGATACCAGGTATCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCCCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAGGTGCAGCATTAAACGGGCCCATTCGTTcc                                                                                     >  1:338996/1‑288 (MQ=255)                                                                                                                                                                                                    gTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTAGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGTTTGTTCGCAACGGGCTTCCCGGGCAGCAGGGGTGTGGAGCATTAACCGGCCCCATTCGACCCGGTgg                                                                                >  2:141307/1‑288 (MQ=255)                                                                                                                                                                                                     tttGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGtt                                                                                                                                                                                                                                                                   <  1:436107/108‑1 (MQ=255)                                                                                                                                                                                                     tttGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGtt                                                                                                                                                                                                                                                                   >  2:436107/1‑108 (MQ=255)                                                                                                                                                                                                     tttGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAACCAGACCTTTGGCATCTTCTAGCAGCCATGCCGGCGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGCATGGTCAGCATGAAACGTCCCCCTTCGCTCCAGTgcc                                                                               >  2:63254/1‑286 (MQ=255)                                                                                                                                                                                                     tttGAAACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGGACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGcc                                                                              <  1:284735/289‑1 (MQ=255)                                                                                                                                                                                                          tgccAGGTTCCATACTGGCGATGCCCAGGAACGGGGGAAGGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAAGCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGgtg                                                                         <  2:251490/287‑1 (MQ=255)                                                                                                                                                                                                            ccAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGgtg                                                                         <  1:262172/287‑1 (MQ=255)                                                                                                                                                                                                             cAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAg                                                                                                                              <  1:487955/233‑1 (MQ=255)                                                                                                                                                                                                             cAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAg                                                                                                                              >  2:487955/1‑233 (MQ=255)                                                                                                                                                                                                               ggTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTAcc                                                                     >  1:425579/1‑288 (MQ=255)                                                                                                                                                                                                                          ggCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCCGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGAGGTGCAGCATTAACCGGCACCATCCCATCCACTGCCCTTGTGTACCCCCAAAcgctcg                                                         >  2:6158/1‑289 (MQ=255)                                                                                                                                                                                                                          ggCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCCGTGGCCTGGTGTACCTCCGAAcgcgag                                                         >  1:47442/1‑287 (MQ=255)                                                                                                                                                                                                                           gCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTTCAGCATTAAACTGCCCCCTTCGATCCACTGGCCTGGTGTCCCTCCGAAccgccgg                                                        >  2:455763/1‑283 (MQ=255)                                                                                                                                                                                                                                      ttaCGGTGGAGCGCGCTTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCATTCAACGTTATAAACAATCCCGTCCGCTCCATCGGGTGCCCAGGCATCGCTCACGGTGGCGTTAAACAGACCGTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCAGTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATgcg                                              <  1:247108/286‑1 (MQ=255)                                                                                                                                                                                                                                      tGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaaa                                                                                                     >  1:669770/1‑233 (MQ=255)                                                                                                                                                                                                                                      tGACGGGGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaaa                                                                                                     <  2:669770/233‑1 (MQ=255)                                                                                                                                                                                                                                       gACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTAGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATgc                                               <  1:141307/286‑1 (MQ=255)                                                                                                                                                                                                                                            tggagagCCCGTAGGCCGTACCCATTGCTTCGACGCTAGGCCACATCAAACCTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGGATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGt                                        <  2:436374/285‑1 (MQ=255)                                                                                                                                                                                                                                                                cccATTGCTGCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTACAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCa                   <  2:383323/289‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttGCTTCGACGATAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGCTGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGAGCGGAAGCGATGCGCCGGGTCATCTTTTGTGTAATCCGGCAGCgg               >  1:239036/1‑289 (MQ=255)                                                                                                                                                                                                                                                                          cgacgaTAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAATCGATGCGCCGGGTTATCTTTGTTGTAATCAGGCAGCGGAttac          >  1:397943/1‑286 (MQ=255)                                                                                                                                                                                                                                                                            acgaTAGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCAGCGGATTcc          >  1:240231/1‑286 (MQ=255)                                                                                                                                                                                                                                                                             cgctAGGCCAACTTACACGGTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCAGCGGATTCCACt       <  1:377088/285‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aGGCCAACTTAAACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCAGCGGATTCCACTGGGTa  >  1:482641/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         AGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATATCCTGTTTGCCGTCCCAGACTTTGGTGGTGACCTTATTGTCCGCATCCAGCTCCTGCCACCAGGAACCATTTTCATAGTCCATCAGGTACTTAATGCAGTACTCCCACCATGTTTGATACCAGGTTTCATACTGGCGATCGCCAGTGACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACGCGCGGAAGCGATGCGCCGGGTTATCTTTGTTGTAATCCGGCAGCGGATTCCACTGGGTA  >  NC_000913/4070534‑4071094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑