Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,613,473	G→T	D355Y (GAT→TAT)	yjjU →	putative patatin‑like family phospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,613,473	0	G	T	100.0%	117.9 / NA	40	D355Y (GAT→TAT)	yjjU	putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/19);  total (21/19)

CCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGC  >  NC_000913/4613212‑4613718                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ccgAAGCCATTACATAGTATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGAtt                                                                                                                                                                                                                                          >  1:373550/1‑275 (MQ=255)      gCCATTACATAGGATGCGGTTTGGCGGTCGGATTCCGGCGCTGCGTGAAGACTATAAAAGTGGGCGTTTAGGCGGTCGTTATTTCCTCGCCCCGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGt                                                                                                                                                                                                                                    <  2:200752/276‑1 (MQ=255)                  tATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGGTTTATCCACAcc                                                                                                                                                                                                                        >  1:241976/1‑276 (MQ=255)                       cgcTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCCTCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCCCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTAGCCACCCCCCCt                                                                                                                                                                                                                    >  2:192186/1‑275 (MQ=255)                            ggCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCCACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTCACAATGAGTATCTGGATTGATTTGCCTTTTTATCTACCCCCACTGCCAtt                                                                                                                                                                                                              >  1:195108/1‑276 (MQ=255)                            ggCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGCCGCTCCGCAGGCGACCGACCCGACATTTAACAATAAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCAtt                                                                                                                                                                                                              >  1:26139/1‑276 (MQ=255)                                 tCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATAGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGACCGACCCGACATTTAACAATGAGTATCTGGTGTGATTTGCCGTTTTCTCGACCCCCACGACCATTTTgca                                                                                                                                                                                                         >  1:392566/1‑274 (MQ=255)                                     agtCCGTCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTGTTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGGGACGCCGGAATCGATTGTCATGCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCGCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTcc                                                                                                                                                                                                     <  1:18618/274‑1 (MQ=255)                                       tCCTGCGCTGTGGGAAGACTTGAAACTTGGGCGTTTAGTAGTTCGTTTTTTCCCCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTGACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCccg                                                                                                                                                                                                   <  2:186166/276‑1 (MQ=255)                                                    gaatataagaaaCTGGGGCGTTTGTGGGGGGGTGATTTCCTGCCGACGGGGTGAACGCTATTAACTGAAAAATCGCCGCTGACCCGCCATGTGGGGTCAGTGGGGACGCCGGAATCGATTGGCATGCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCg                                                                                                                                                                                      <  2:341656/267‑1 (MQ=255)                                                           ataAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAag                                                                                                                                                                                >  1:274367/1‑275 (MQ=255)                                                               aCTTGAGCGTTTATGCGGTCGTTCTTGCCCCGCCACGGTTGGCAAGCGATTAACGGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGc                                                                                                                                                                            <  2:373550/275‑1 (MQ=255)                                                                    ggCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGCGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGACGCGGcacgcc                                                                                                                                                                       >  1:176206/1‑270 (MQ=255)                                                                        tttATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCa                                                                                                                                                                   >  1:60485/1‑275 (MQ=255)                                                                            ttgggTCGTTATTTGCTCGCCACGGTTGGCAAGCTATTAACGGAAACAGCGCCGCGTACCTGGCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCGGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGGTTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAAcgcg                                                                                                                                                              <  1:155846/273‑1 (MQ=255)                                                                                               gcACGGTGGGCAATCTCTTAACGCAAAAAGCGCCGCTTACCCGCCGTCTGGGGCCAGTGGTGCCGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAgg                                                                                                                                           <  2:353786/275‑1 (MQ=255)                                                                                                cccGGTTGGCAAGATATATACTGAAAAAGCTCCGCTTCCCCGCCATCTGTGGCCAGTGGTGAGCCCGGAATCGATTGTCATTCCGCCTGCGCCAGTAGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGc                                                                                                                                          <  2:56401/274‑1 (MQ=255)                                                                                                         cAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGGGGCCAGCCTGCAACGCGCGGCCCACGCGGGCGGCGGCCAGATCagt                                                                                                                                 >  2:155013/1‑274 (MQ=255)                                                                                                             cTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCGGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTGTTATCGACACCCACTGCCATTTTGATTTCCCGCCTTTTAGTGGCGCTGAGGGGGCCAGCCTTGAACGCGAGGCACAACCGGGCGTAGGCAAGAtaactttgc                                                                                                                             >  2:227964/1‑268 (MQ=255)                                                                                                                       aaaaaGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCATGCCTGCAACGCGCGGCACCAGCGGGCGTAAGCAAGATCATTGTTACGGCAACagc                                                                                                                   >  1:271914/1‑275 (MQ=255)                                                                                                                                      cccGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGc                                                                                                    <  1:37154/276‑1 (MQ=255)                                                                                                                                       ccgcaagcgggTGCCCGTTGTGGCGCCGGAATCCATTGTCATGCCGCCGGCGCACGTCGCCAACGAGCCGCGGGTTGCCGAAGTGAGGGCCGCTCCGCAGGCGAACGACCCGACATTGAACAAGGATTATCTGGCTTGATTTGCCGTTTTATCGACACCCCCTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGcc                                                                                                   <  2:22116/267‑1 (MQ=255)                                                                                                                                               tgtggCCGGTGGTGCGTCCGCAATGGATTGTCATTACGCCGGCGCCCGTCGCCAACGATACGCTGGTGGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGCCATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGtt                                                                                           <  2:74316/272‑1 (MQ=255)                                                                                                                                                       gtggtgACTCCGGAATCTCGTGTCATTCCTCCTGCGCCAGTCTCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGTAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGGTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAg                                                                                   <  2:56589/276‑1 (MQ=255)                                                                                                                                                       gtggtgACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTGTAGTGGCGATGAAGAAGCCAGCCTGCAACGCGCGGCACAAGCGGGCGGAGGCAAGATCATGGTTCCGGCACCAGCGGCCGAAAATTCTGAGCGTTCGTTGGCAtttg                                                                                   >  1:55123/1‑274 (MQ=255)                                                                                                                                                        tggtgACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTACGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATGTCCCGCCGTTTAGTGGCGATGAGGAGGCCAGCCTGCAACGAGCGGCACAAGCGGGCGTAGGCCAGATCATTGTCCCGGCAACAGAGGCGGAAAATTTTGGCCGCGCGTTGGCATTAg                                                                                   >  1:6531/1‑275 (MQ=255)                                                                                                                                                                    aaccGATTGTCATGCCCCCTGCGCCAGCCGCCAACGATACGCTGGTGGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATc                                                                       <  2:60485/272‑1 (MQ=255)                                                                                                                                                                      tCGATTGTCAGTCCGCATGCGCCAGTCGCAAACGATACGCTGGTTGCGGAAGTGAGCGACGCTCCGAAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCaa                                                                     <  1:15576/275‑1 (MQ=255)                                                                                                                                                                         aTTGTCAGTCAGCCTGCGACCGTCGCCAACGATACGCTGTTTGCCGAATTGAGCGACGCTCCGCAGGCGAACCACCCGCCTGTTAAAAATGAGTATCTGGCTTGATTGGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGAGGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCg                                                                  <  1:331690/275‑1 (MQ=255)                                                                                                                                                                                      cTGCGTCAGTCGCCAACGATACGCTGGTTGGCGAAGTGAGCGACGATCCGCATGCGACCGACCCGACCTTTAACAATGAGTATCTGGCTTGATTTGCCGTTGTATCGACACCCACTGCCATTTTGATTGCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCAt                                                     <  1:192186/275‑1 (MQ=255)                                                                                                                                                                                               tCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTCGCGGCAAATTATCACCCGCTGTATGCAGCATTGGGCTTGCa                                           >  1:244588/1‑276 (MQ=255)                                                                                                                                                                                                   aaactgtACGCTGGTTGCTGAATGGAGCGATGCTCGGCAGGTGAAGGACCCGATATTTATCTATGATTATCTGGCGTGCTTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGGAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATcc                                        <  1:236413/269‑1 (MQ=255)                                                                                                                                                                                                                gTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGCAAATTTTGCCCGTGTGTTGGCATTa                                                                                    >  1:238019/1‑218 (MQ=255)                                                                                                                                                                                                                gTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAACTTTTGCCCGTGTGTTGGCATTAACGGAGAATTATCACCCGCTGTAGGCCGCATTGGGCTTGCCTCATGGTATGTTGGaca                          >  1:318015/1‑274 (MQ=255)                                                                                                                                                                                                                 ttGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCGGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGATTGCAGCCTGGTATGTTGGaaaa                         >  1:261058/1‑276 (MQ=255)                                                                                                                                                                                                                    ccGAAGGGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACa                      <  1:223620/276‑1 (MQ=255)                                                                                                                                                                                                                          tGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCg                 >  1:239302/1‑275 (MQ=255)                                                                                                                                                                                                                              cgcggCTCCGCAGGCGAACAACCCGACATTTAACAATGAGTATCGGGGTTGAGTTGCCGTTGTATCGACACCCACTGCCATTTTGATGTCCCGCCGTTTAGGGGCGAAGAAGAGGCCAGCCTGCGACGCGCGGCACAAGAGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGAtgt             <  2:271914/271‑1 (MQ=255)                                                                                                                                                                                                                                    tCCGCAGGCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACACGCGGGCCGAGGCAAGATCAGTGTGCCGGCAACAGAGGCGGAAAATGTTTCACGGGAGTTTGCATCAGCGGAAAAGGCACAAACTCGGTATGCAGTCTTGGTCTGGCATACTGGTGCGTGGGAAAGACACGGCGATgtgttgctt      >  2:368454/1‑271 (MQ=255)                                                                                                                                                                                                                                           gCGAACGACCCGACATTTAACAATGAGTATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGagc  >  1:210266/1‑273 (MQ=255)                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACCCGACATTTAACAATGAGGATCTGGCTTGATTTGCCGTTTTATCGACACCCACTGCCATTTTGATTTCCCGCCGTTTAGTGGCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGC  >  NC_000913/4613212‑4613718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑