| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,529,203 | C→A | pseudogene (1302/2037 nt) | rhsE → | pseudogene, Rhs family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,529,203 | 0 | C | A | 88.9% | 41.4 / NA | 27 | pseudogene (1302/2037 nt) | rhsE | pseudogene, Rhs family |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (9/15); minor base G (0/3); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAG > NC_000913/1529042‑1529468 | ctgGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCg < 1:291886/275‑1 (MQ=12) gaaATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCat > 1:359134/1‑276 (MQ=12) aggccGCGGGAGGAGTGAAAGGCGAGGGGGGCTGGCGCAGGGGGGGGTGACGGTGGGGCAACTGGCCAGACATGTGGAGCCGGAATAAAAACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt < 2:92109/272‑1 (MQ=11) agacgggttgggCAGTGAAAGCGGGGCGTGGCGTGCGCAGGGGGGGGTGAAGGGGGAGCAAATGGCCAGAAAGGTGGAGCCGGAATACACACCGGGGCGAAAAGCACAGCTTTAGCACGGCGACCAACGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGt < 2:364728/264‑1 (MQ=11) cgcgtgggtgtggAAAGCCGGGGGTGGCTGGAGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCgggg < 2:396415/264‑1 (MQ=11) gggTGGGCAGTGAAAGCCGGGGGTGGCGTGCGCAGGGCGGGCTGACGGGGGAGAAAATGGCCAGACAGGTGGGGCCGGAATAAACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGc < 2:377437/274‑1 (MQ=11) gTGAGCAGTGACAGCCGGTCGTGGCTTGCGCAGTGCGGGCGCACGGTGGAGCACCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTg < 1:146061/276‑1 (MQ=11) caggggaaGGCGGGCGGGGCTGGCGCAGTGCGGGCAGACGTGTGAGCAACTGGCCATACAGGTGGAGCCGGATGCCACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGTGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACt < 1:293775/269‑1 (MQ=11) cAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGCATCACCTGCACCACCCGTACCGTCTGCCAGGGCAGCAGCATGATGCGGAGTCGGGGGGGTACt > 2:348356/1‑275 (MQ=11) gggCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCAc < 1:78989/275‑1 (MQ=255) gtgggCTTGCGCAGTGCGGGCGGACGGGGGAGCAACTGGCCTGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCgg < 2:404859/272‑1 (MQ=255) cggggatggcgcAGTGCGGACTGACTGTGAGGAAAAGGGCAAGACAGGTGGACCAGGAATACACACCGGCGCGAAACGCTCATATTTATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCgg < 2:37299/267‑1 (MQ=11) ttGCGCAGTGCGGGCTGACGGTGGAGCAACGGGCCGGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCCCGGCGACCACCGGGGACTGCCGCTGGCGCTGATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGGCACCGGCACTACg < 1:20099/276‑1 (MQ=11) cTGACGGTGGGGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAgggg < 1:16818/276‑1 (MQ=255) ggACGGTGGATCAACGGGCCAGACAGGTGGAGCCGGAATACACCCCGGCTCGAAAATCTCATCTCTATCACCGCGAGCACCGGGGACTGCCGCTGGCGTTTATCATCTAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAgggg < 1:150579/274‑1 (MQ=11) cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGTGGCGCGGGGCGTCTGATGAATGGGGCAACCAGCTTAATGAGGAGAGCCCGCATCACCTGAACCAGCAGTACATTATGCCAGGGCAGCCGCATGATGAGCAGTCGGGTCTGACCTATACCCGGCTCAGCCACTCGCATCCGTTGCAGggggggg > 2:241407/1‑275 (MQ=11) cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCCGCAGACTGACGCGGAGTCGGGGCTGTACTATAACCGTACCCGGCACTACGACCCGTTGCCGGGGCgg > 2:252601/1‑275 (MQ=11) cggCCAGACAGGTGGAGCCGTAATCCACAGCGGCGAGAGAAGCTCATCTTAACCACTCCGACCGCCGAGGGCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATGCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTAGCAGGGGCGGTATATCACCCCgg < 1:132351/275‑1 (MQ=14) caCATGTGGAGCCGAAATGACCGCCGGCTCTACAAGCGCATCTTTTGCACTGCCACCACCGGGGAGTGCCGCTGGCGCTGATCAGCGAAGACTGCAAGACGGCGTGGCGCGGGGAGTATGATGAATTGGTCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGa < 1:174309/275‑1 (MQ=14) ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCCCCGGCACTCCGATCCGTTGCAGGGCGGGTTTAACACCCCGTGCCCCGTAGGGTTgaga > 2:404191/1‑276 (MQ=14) aTACACGCCGTCGCGAAAAGCGCGTCTTTGACACTGTGACCGCCGGGGGATGCGGCGGGTGCGTATCAGGGAAGCCGGCAATACGGCGGGGGGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGGGGGGAGCCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGtgg < 2:332217/276‑1 (MQ=14) cGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACAGTCACCGGCACTACGCTCAGTGGCAGGGGCGGTATATCCACCCGGACCCGATTGGGGTGGGCggtgggtgtgcgc > 2:330822/1‑271 (MQ=14) acctgtcgcagtGCGGCCACCGCGGGCGGCCGCGGGCGATGATCATCGAAGACGGCAATACGGCGGGGCGCGGGGAGTATGAGGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCg < 2:92010/264‑1 (MQ=17) gcggacccgggggACTCCCGCTGGGGCCTAGCAGGGAAGACGGAGATGCGGCGTGGCGCGGGGAGTATGCTGGATGGGGCAACCATCTTAATGAGGAGAACCCGCATCCCCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATAc < 2:375242/267‑1 (MQ=17) gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGGACTATAACCGTCACCGGCACTACGAGCCGTTGCAGGGGCGGTATATCACCCCGGACACGACTGGGGTGCGAGGTGGCTGGACTAGGTCTCAGTCTCCGTTGAATccccagc > 2:328412/1‑272 (MQ=17) cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGCTTGGGTTGAGTGGTGGAAGGAATATGTAAACGTCTCCGTTGACTCCCCTACa > 1:99090/1‑276 (MQ=17) accaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCCCCCCGGACCCGATTGGGTTGAGAGGTGGAGGGAATATGTATCAGTATCCGTGGATACCCATAAAAg > 2:311360/1‑276 (MQ=255) | CTGGACAGGCTGGAGGAAGAAATCCGGGCAGACCGCGTGAGCAGTGAAAGCCGGGCGTGGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGTTCATTTTTACCACTGCGACCACCGGGGCCTGCCGCTGGCGCTCATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAGTCGGGGCTGTACTATAACCGTCACCGGCACTACGATCCGTTGCAGGGGCGGTATATCACCCCGGACCCGATTGGGTTGAGAGGTGGATGGAATATGTATCAGTATCCGTTGAATCCCATACAAG > NC_000913/1529042‑1529468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |