Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,859	C→T	intergenic (‑50/‑53)	dmlR ← / → dmlA	transcriptional activator of dmlA/D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,859	0	C	T	96.3%	61.4 / NA	28	intergenic (‑50/‑53)	dmlR/dmlA	transcriptional activator of dmlA/D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (14/12);  minor base G (0/1);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTA  >  NC_000913/1881589‑1882110                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            cGCTGCGCCCTTGCCTACATCTGCTCTCCTTCTTCGGTAATGGTGTCGCGGCGGTTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCCCCAGCAAGGCGATGCGTTTGCTGACGAGCGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGTCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCCCCAGTTACGGGGATTAATTCCTTTTCAGTTAATta                                                                                                                                                                                                                                                        <  1:303050/276‑1 (MQ=255)                         tctcCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTCCATTATTCATGCTTCGTGTGTTATGTTTCACCAGTTACGGGGATTAATTCATTTTCAGTTACTTATAGGAGGGTGagtttgcagatttt                                                                                                                                                                                                                                >  2:11206/1‑263 (MQ=255)                             cttcttCGGTAAGGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTCTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATg                                                                                                                                                                                                                            <  1:206676/276‑1 (MQ=255)                                           gTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGTTGTTGTCCATTTTGATGGTCAGGAAGTgaga                                                                                                                                                                                                              >  1:294437/1‑276 (MQ=255)                                              aCGTGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCACGACTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTTAATTTTGATGGTCAGGATGTGAGAAcc                                                                                                                                                                                                           >  1:313008/1‑276 (MQ=255)                                                                          ccacGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATtgctgc                                                                                                                                                                               >  1:117664/1‑276 (MQ=255)                                                                                  aGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCg                                                                                                                                                                        >  1:218827/1‑275 (MQ=255)                                                                                     gTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACCCGCAAATCATTCAGCAGCGGTAAATCATTCCTGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTACTTATAGGATGTTGATGTTGTTAATTTCTATGGTCAGTACGTTACAACCCACTTATAAACCCGTACCGTATTTCTGCGCCCCCgtcca                                                                                                                                                                    >  2:85718/1‑272 (MQ=255)                                                                                        tGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAATTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACg                                                                                                                                                                 >  2:314033/1‑276 (MQ=255)                                                                                               gCAAGGCGATGCGCTTGCTGACGAACGCCGGGGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTgg                                                                                                                                                          <  1:198752/276‑1 (MQ=255)                                                                                                                            cgTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGGCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGGTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTcgcg                                                                                                                             <  2:400774/275‑1 (MQ=255)                                                                                                                             gggAAGCGCCCAGCTGTTCCGCCACGGCGGCAAAACCAGCCCGGCTAGACACCAGCATATAGACGAGCAAATCATTCAGCAGCGGTAAATTATTCATGAGTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCGTTTCAGTTAATTATAGGATGGGGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAAACCAATTATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTcgcg                                                                                                                             <  2:72876/273‑1 (MQ=255)                                                                                                                                    gCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACa                                                                                                                      <  1:7042/275‑1 (MQ=255)                                                                                                                                      ccAGTTCTTCCCCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGAAGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGc                                                                                                                   <  1:312853/276‑1 (MQ=255)                                                                                                                                              gccgcaccggcgtCAAATCCAGCCAGGCGAGCCACCAGCATAAAGACGCGCAATTAATTCAGGAGCTGTAAATCATGCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTg                                                                                                           <  1:310044/269‑1 (MQ=255)                                                                                                                                                            aaaaCCAGCCCGGAGAGGCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGTTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAAGTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTc                                                                                             <  2:376478/276‑1 (MQ=255)                                                                                                                                                                 cAGCCCGGCGAGCCACCAGCATAAAGACGCTCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTGAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCtt                                                                                        <  1:357586/276‑1 (MQ=255)                                                                                                                                                                        gCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCCTGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGATAATTCTAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTTCTGCGATCCAGGGCGCCGGGATTGGCAAATACGTCCTTCCTGAAGGGATACGCGTGTGACAGGCTGCCGCTGAGCGCTGGGGCTGCGCCGTGAGt                                                                                    >  2:355580/1‑273 (MQ=255)                                                                                                                                                                         gtaGCCACCAGCATAAAGAAGCGCAAATCATTCAGCAGCGGTAAATTGTTCATGATTCGTGTTTTATGGTTCACCAGTTACGGGGAGTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTg                                                                                <  2:164753/274‑1 (MQ=255)                                                                                                                                                                         cGAGCCACCAGCATAAAGGCTCGCAAAACATTCAGAAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGTGGATTAATGCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCTAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTg                                                                                <  1:276176/276‑1 (MQ=255)                                                                                                                                                                                              cgcAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGCTCCCGGGAGACGTGATTGGCAAAGCAGTCCTTCCTGCAGGGCTTCGCGTGTTACGGGCTCCCGCTGAGCGCTGGGGCTTCGCCTTGCCTTTTGAGCAATTGGAGTGGGTGAGCt                                                           >  2:226079/1‑276 (MQ=255)                                                                                                                                                                                                  aaTCATTCAGCATCGGTAAATTATTCATGATTCGTGTGTTATGTGTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGGGATGTTGTCAATTGTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGc                                                         <  1:143646/274‑1 (MQ=255)                                                                                                                                                                                                          agtaggggTAAATTATTCATTGTGCGAGTTTTATGTATACCCAGTTACGGGGATTGATTCCTTTTCAGTTAATTAGAGGATGGTGATGTTGTCAATTTTGATGGTCAAGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACa                                               <  2:61783/270‑1 (MQ=255)                                                                                                                                                                                                                              gATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGGGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTCCAGACATCACGGTAGAatgatg                           >  1:57683/1‑276 (MQ=255)                                                                                                                                                                                                                              gATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAatgatg                           >  1:256307/1‑276 (MQ=255)                                                                                                                                                                                                                              gATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCGGCGGGTATTACAGCCATCACGGTAAAatgatg                           >  1:214507/1‑276 (MQ=255)                                                                                                                                                                                                                                    tgtTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGCGTTTTGCGCAAATGGAGTGGGCGGGCTGGGATTATTACTGCAATCACGGTAAAatgtgtccgtcc                     >  2:37270/1‑267 (MQ=255)                                                                                                                                                                                                                                                       tACGGGGATTAATTCCGTTTCAGTTAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGACCTTCTTGAAGGGACTAGCGTGTTACAGGATGCCGCTGAGGGCTGGGCATTCGCCTTGAGTTTTGTGCAAGTGGAGTGGGCGATCTGCGGGTATTACGGCCATCCCGGTCAACTGAGACCGGCCGACTGGCATGAGCacgttc  >  2:200289/1‑271 (MQ=255)                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            CGCTGCGCCCATTCATAAATCCGCTCTCCTTCTTCGGTAATGGTGACGCGGCGGGTGGTGCGGTGTAACAACACCACGTTTAGCGTTTGCTCCAGCAAGGCGATGCGCTTGCTGACGAACGCCGGTGAAACGCCCAGTTCTTCCGCCACGGCGGCAAAACCAGCCCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTA  >  NC_000913/1881589‑1882110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑