Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,097,158	C→T	9.8%	intergenic (+329/+407)	ycdU → / ← serX	putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,158	0	C	T	9.8%	186.2 / 3.3	83	intergenic (+329/+407)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (34/38);  new base T (2/7);  total (37/46)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCC  >  NC_000913/1096859‑1097456                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                             atatatGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATac                                                                                                                                                                                                                                                                                                              <  1:877101/300‑1 (MQ=255)    tatGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACg                                                                                                                                                                                                                                                                                                           <  1:567699/300‑1 (MQ=255)       gTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑aaa                                                                                                                                                                                                                                                                                                       >  1:261774/1‑300 (MQ=255)         tCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTTTCGACCCCCGGTCGGGGCTCCTCATCCCCCAGGTGTGT‑TCAATCCACG‑caaaa                                                                                                                                                                                                                                                                                                     >  1:1299540/1‑300 (MQ=255)           tGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATTTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGTTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑aaaaaaa                                                                                                                                                                                                                                                                                                   <  1:276433/300‑1 (MQ=255)           tGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTACAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑aaaaaaa                                                                                                                                                                                                                                                                                                   >  1:1427801/1‑300 (MQ=255)                     tGCGCATTTTGATTTTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑aaa                                                                                                                                                                                                                                                                                                       <  2:276600/286‑1 (MQ=255)                             ttGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACg                                                                                                                                                                                                                                                                                 <  1:241762/300‑1 (MQ=255)                              tGATTGTTGACGGAGTAACCAGACAGTGGATGTGAACGATTTCCCCTCGCCCTAACAGACGGGGGCGGGGGCACCCGGTGGGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGa                                                                                                                                                                                                                                                                                <  1:618255/300‑1 (MQ=255)                               gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTTTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTGCTCATCCCCCCGGCGTGT‑GGCATACACG‑ACAAAAACGccc                                                                                                                                                                                                                                                                                              >  2:568923/1‑285 (MQ=255)                                  tgttgACTGGGTAACAAGACAGTTGATGTGAACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTGCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTAc                                                                                                                                                                                                                                                                                          <  2:761551/286‑1 (MQ=255)                                      gACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCACGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCtc‑ttct                                                                                                                                                                                                                                                                       <  1:99928/300‑1 (MQ=255)                                        cTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAACCCGCTCAATGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCACCCCGGTGTGT‑GCAATAACCG‑AAAAAAAGACCCGTACTTTCGTACGAGATC‑TTCgtt                                                                                                                                                                                                                                                                     >  1:521308/1‑300 (MQ=255)                                             tAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTCCTTTCTTACCAGCTCTTTCTTTaa                                                                                                                                                                                                                                                                   >  1:152137/1‑298 (MQ=255)                                             aaaCAAGACAGTTGAGGTGCACGATTTCCCCACGCCTGAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAat                                                                                                                                                                                                                                                                 <  1:1332643/298‑1 (MQ=255)                                              aaCCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAAGGTCTGTCCAACTGGCTGTCGCCAGATGGCGAGACCCCGTCTGGGCTTCTCCTCCCCCCGGTGAGT‑GCAATACACG‑CAAAAAACGCCCGTACTTCCGTAcacgt                                                                                                                                                                                                                                                                              >  2:463224/1‑282 (MQ=17)                                                         tGATGTGCACGATTTCCCCTCGCCCTACCGGACGTGGGCGGGGGCACCCGGGGTGGGAAAAATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCgg                                                                                                                                                                                                                                                          <  1:1171103/294‑1 (MQ=255)                                                         tGATGTGCACGATTTCCCCACGCCCTAACAGACGGGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAggg                                                                                                                                                                                                                                                    <  1:563525/300‑1 (MQ=255)                                                           aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAggggg                                                                                                                                                                                                                                                  >  1:649641/1‑300 (MQ=255)                                                            tgtgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCGTCGCAGGCCCGGCGGGCGGCACGCTCACTGAGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCAACCCCCCGGGGTGT‑GCAATACACG‑AACAAAAAGCCCGTAATTTCGTACGAGCTC‑TTCTTTAAatat                                                                                                                                                                                                                                                               >  2:545741/1‑286 (MQ=17)                                                             gtgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAggggggg                                                                                                                                                                                                                                                >  1:679404/1‑300 (MQ=255)                                                               gCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACGAAAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGa                                                                                                                                                                                                                                               >  1:1289394/1‑300 (MQ=255)                                                               gCACGAGTTCCCCTCGCACTAAAAGACGGGGGCGGGGGCACCCGGTTTGGTCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTGACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGc                                                                                                                                                                                                                                                            <  2:1233845/286‑1 (MQ=255)                                                                 aCGATTTCCCGTCGCCCGCACAGACGTGGGCGGGGGCACTCGGTGAGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGGGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTg                                                                                                                                                                                                                                            <  1:1179979/300‑1 (MQ=255)                                                                  cGATTTCCCCTCGCCCTAACCGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                                                                                                                                                                                           <  1:628453/300‑1 (MQ=255)                                                                            tCGCCCTAACAGACGTGGGCGGGGGCACCCGGGTTGTGTAATATATGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                                                                                                                                                                                                                 <  1:252435/300‑1 (MQ=255)                                                                                  tAACTGACGTGGGCGGGGGCACCCGGTGTTGGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCGGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGccc                                                                                                                                                                                                                           <  1:561210/300‑1 (MQ=255)                                                                                       gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGCCGGGGCTTCTCATCCCCCCGGTGCGT‑GCATTTCAAG‑AAAAAACAGCCCGTACTTTCGTTCGAGCTC‑TTCTTTAAATCTGGCGCTGAGGGTGGGAttttgca                                                                                                                                                                                                                                        >  2:1107488/1‑277 (MQ=11)                                                                                          gTGGGCGGGGGCACCCGGTGGGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGc                                                                                                                                                                                                                    <  1:149343/299‑1 (MQ=38)                                                                                           tGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGTCTTCCCATCCCCCCGGTTTGT‑GCAATCTGCG‑AAAAAAAAGCCCGTACTTTAGTCAGAGCTC‑TTCTTTAACTCTGGCGGTTAGGGGGGGATTTACTCGCTTCGCTTGCACTGCggccc                                                                                                                                                                                                                   >  1:579143/1‑296 (MQ=11)                                                                                            gggCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTTCCGGCAg                                                                                                                                                                                                                  >  1:1184746/1‑299 (MQ=37)                                                                                                ggtggCACCCGGTTTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGc                                                                                                                                                                                                             <  1:630882/297‑1 (MQ=21)                                                                                                     cACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTc                                                                                                                                                                                                           >  1:405605/1‑297 (MQ=18)                                                                                                             gttggCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAGTGACTCGCTTCGCTCGCCGGGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCTACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTGCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa                                                                                                                                                                                                 <  1:534965/295‑1 (MQ=16)                                                                                                                 gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACgg                                                                                                                                                                                              >  1:657980/1‑298 (MQ=33)                                                                                                                  cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTc                                                                                                                                                                                            <  1:990434/299‑1 (MQ=33)                                                                                                                      ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATTTGGCGGTGAGGGGGGGATTGACTCTCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTTGCTGTCGCCAGTTGTCGACCCCCGTTCGGGTCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTcc                                                                                                                                                                                       <  1:156578/300‑1 (MQ=12)                                                                                                                          gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGCGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa                                                                                                                                                                                      >  1:231804/1‑297 (MQ=17)                                                                                                                                   gCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTAAGAGCCC‑TTCTTTAAATATGGCGGTGAGGGGGGGAGTGACTCGCTTCGCTCGCCCTGCGGGCAGCACGCTCACTGGGTTCACGGTCTGTCAAACTGGCTGTCg                                                                                                                                                                           >  1:1205173/1‑299 (MQ=12)                                                                                                                                     ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGccc                                                                                                                                                                        >  1:687902/1‑299 (MQ=17)                                                                                                                                       gtccttgctgaCGCGCTCTTCTTTAAATAGGGCTGTGTGGGGGGGATTGACTCGCTTCGCTCTCCCGGCGTGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCCTTCGACAGTTTTCCCCCCCAGGTCGGTGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAAc                                                                                                                                                                                    <  2:350184/276‑1 (MQ=11)                                                                                                                                          cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCACCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGt                                                                                                                                                                   <  1:384555/300‑1 (MQ=17)                                                                                                                                                          tcttTAAATATGGCGGTGCGGGGGGTATTGACTCGCTTCGCTCGCCCTGCCGGCAGCCCGCTCATTGCATTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGCCGACCCCCGGTGGGGGCTTCGCATCCCCCCGGTGTGT‑GAAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgggg                                                                                                                                                   <  1:980025/300‑1 (MQ=255)                                                                                                                                                           cttTAAATGTGGGGGTGCGGGGGGTATTGACTTGCTTCGTGCGCCCTGCGGGCAGCCCGCTCATGGCGTTCACGGTCTGTCCAACTGTCGGTCGCCAGTTGTCGGCCCCCGGTCGGGGCTTCGCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCGACAGCCAGTTggaca                                                                                                                                                                <  2:123616/286‑5 (MQ=11)                                                                                                                                                           ctgtAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc                                                                                                                                                  <  1:654981/297‑1 (MQ=17)                                                                                                                                                              tcttccgatgtgTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGTGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc                                                                                                                                               <  1:277017/289‑1 (MQ=17)                                                                                                                                                                 atatGGGGGTGAGGGGGGTATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCa                                                                                                                                            <  1:1287941/300‑1 (MQ=17)                                                                                                                                                                    tGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAACAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATc                                                                                                                                          >  1:950871/1‑299 (MQ=17)                                                                                                                                                                       cGGTGAGGGTGGGATGGACTCGCTTCGCTCGCCCTGCGTGCAGCCCTCTCACTGCGTTCGCGGTCTTTCCAACTGGCGGTCGCCAGTCGTCGACCCCCGGTCGGGGCTTCGCAGCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg                                                                                                                                                    <  2:590385/286‑1 (MQ=11)                                                                                                                                                                            gggaggggATTGACTCGCTTCGCTCGCCTGGCGTGAAGCCGGCTCATTGCGTTGACGGTCTGTCCAACTGGCTTTCGCCATGTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAAGACACG‑AAAAAGAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc                                                                                                                                               <  2:598260/282‑1 (MQ=11)                                                                                                                                                                             gggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACTAGCTC‑TTCTTTAAATATGGCGGTGCGGGGGGGATTGACTCGCTTCGCCCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGGCTGTCCAACTTGCTGTCGCCCGTTGTCGAACCCCGGTCCGGGCTTCCCATCCCCCCGgtgt                                                                                                                                >  1:375578/1‑300 (MQ=255)                                                                                                                                                                                 gggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCCGCCCCCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCCGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGgtgtgtt                                                                                                                             >  1:925353/1‑298 (MQ=17)                                                                                                                                                                                  ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGc                                                                                                                            <  1:1371304/299‑1 (MQ=32)                                                                                                                                                                                       gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAACAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCCACTGGCTGTCGCCAGTTGTCGAACACCGGCCGGGGCTTCTACTctcccc                                                                                                                                     >  2:1253865/1‑285 (MQ=12)                                                                                                                                                                                       gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTTGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAatat                                                                                                                       >  1:1058957/1‑299 (MQ=17)                                                                                                                                                                                         cTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACg                                                                                                                    >  1:808615/1‑300 (MQ=32)                                                                                                                                                                                               tcgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGaaaaaa                                                                                                              >  1:649907/1‑300 (MQ=32)                                                                                                                                                                                               gcgctcgcCGTGTGGGCATCCCGCTCACTGCGTTCACGTGCTGTCCAACTGGCGGTCGCCATTTGTAGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAAAACACG‑AAACAAAAGCCCGTACTTCCGTCCGGGCGC‑TCCTTCGAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGtgtgtg                                                                                                                             <  2:820944/284‑1 (MQ=11)                                                                                                                                                                                                  cccgcCCTGCGGGAAGCCGGTTCAGTGCGTTCACGTTCTGTCCCACTGGCTGTCGCCAGGTGTCGACCCCCGGTCGGGGCTTCTCATCCCGCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAg                                                                                                           <  1:43858/298‑1 (MQ=255)                                                                                                                                                                                                    cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGc                                                                                                          >  1:990623/1‑299 (MQ=32)                                                                                                                                                                                                       ccggCGGGCCGCCCGCGCTCTGCGTTCACGGTCTGTCCAACGGGCGGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACg                                                                                                                    <  2:699782/283‑1 (MQ=12)                                                                                                                                                                                                               cAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGt                                                                                              >  1:762321/1‑300 (MQ=32)                                                                                                                                                                                                                    cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCCCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTTACGGTCTTTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGGTTGCAATATAAGAAACAAAAGCCCGTCCTTTCGtccca                                                                                          >  1:362680/1‑295 (MQ=255)                                                                                                                                                                                                                        cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCCCCGCGTTCCCCGTCTGTCCCACTTGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAACCCGTCCTTTCGCACGAGcctt                                                                                     >  1:6088/1‑297 (MQ=11)                                                                                                                                                                                                                                               tcggCTGTCTCCAGTTGTCGACCCCCGTGCGTGCCTTCCCCTCCCCCGGGTGTGT‑GAAATACACG‑AAACAAAAGCCCGTTCTTCCGTCCGAGCTC‑TTCTTTAAAGATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAat                                                                            <  2:912207/284‑1 (MQ=11)                                                                                                                                                                                                                                                          cAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtc                                                   >  1:95367/1‑300 (MQ=33)                                                                                                                                                                                                                                                               ttCGACGCCCGGGCGGGTCCTCTCATCCCCCCGGGGTTT‑GCAATATACG‑AAAAAAAAGCCCGTGCTTTCGTACGAGCTC‑TTCTTTAAATAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                              <  1:646109/299‑1 (MQ=255)                                                                                                                                                                                                                                                               gtttaCCCCCGGTCGTGTCTTCTCCTCCCCGCGTTTTCT‑GCAATATACG‑CAAAACATGCCCCGTCCTTCTTACGTTCTC‑TTCTTTAAATGTGTCGGTGAGGGGGGGATTTACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                              <  1:973951/296‑1 (MQ=11)                                                                                                                                                                                                                                                               gTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAAGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                              >  1:957259/1‑300 (MQ=25)                                                                                                                                                                                                                                                                 cGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGTTTGACtcgcttcg                                             >  1:599950/1‑299 (MQ=25)                                                                                                                                                                                                                                                                 cGAACCCCGGTCGGGGCTTCTCACCCCCCCGGTGTGT‑GCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                            <  1:225260/300‑1 (MQ=17)                                                                                                                                                                                                                                                                  gACCCACGGTCGGGGTGTCTCAACCACCCGGTGTGT‑ACAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCGGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttcgc                                            <  1:817753/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     gcccGGTCTGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcgc                                        <  1:1415834/299‑1 (MQ=37)                                                                                                                                                                                                                                                                     ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACGAAAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCGCCCCGGTGTGTGCAATGTACGGAGTAAAATACCTTACGTTTGTACTGGCCCTCTTTTAAATATGACGGAGAggtggggtac                                                        >  2:213093/1‑282 (MQ=11)                                                                                                                                                                                                                                                                           tCGGGGCTGATCATCCCCCCTGGGTGA‑AAAATATACA‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAAGATGGCGGTGAGGGGGAGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                  <  1:1204790/300‑1 (MQ=11)                                                                                                                                                                                                                                                                               ggCTTCTCTCCCCCCCGGTGGGG‑GCAAAATACG‑AAAAAAAAGCCCGTACTTTCGTACGGGCTT‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGc                               <  1:721847/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   tctcATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCCCTTCCTTCAATACGGCGGTGAGGGTGGGATTGACTCTCTCAGCTCCCCCTGCCGGCAAcc                           >  1:1007884/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                   tctcACCCCCCCCGTGTGTAGAAAAAAAGG‑AAAAAAAACCCCTTATGTTGGGACGGCGTC‑TTCTTTAAATATGGCGGTGAGGGGGGGGTTGACCCGCTTCCCTCGCCCTGCGGTCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcg                                         <  2:663012/286‑1 (MQ=11)                                                                                                                                                                                                                                                                                   tctcAACCCCCCGGTGTGT‑GCAATATACG‑AAAAAAAAGCCCGTACTTGCGTACGAGCTC‑TTCTTTAAAGATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGGTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCGGccc                          <  1:1363384/300‑1 (MQ=16)                                                                                                                                                                                                                                                                                             ccgttttgt‑gCAATTTACG‑AAAAAAAAGCCCGTTCTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTTCCTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCTGCCCGcagggcgag                <  1:210813/294‑9 (MQ=11)                                                                                                                                                                                                                                                                                             ccGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGCCGGGGCGTGTCATCCCCCCGGTGTGTGCAATCTACGCAAAAAAAGCCCGTACTTTCGTCCGAGCTCCTCTTTAAATATGGCGGTGAGGGGGGGATCTACTCGCTTCGCTCGTCCTCCGGGcc                              >  2:1146779/1‑285 (MQ=11)                                                                                                                                                                                                                                                                                               ggTGTGT‑GCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCCGCCCGCTCACTGCGt               >  1:1452611/1‑299 (MQ=14)                                                                                                                                                                                                                                                                                                       tcAATACACG‑AAAAAAAAGCCCGTACTTTCTTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGCGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTc       <  1:1392431/299‑1 (MQ=18)                                                                                                                                                                                                                                                                                                           tACACG‑AGAAACAAGCCCGTTCTTTCGTACGGGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGTATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc   <  1:477557/300‑1 (MQ=16)                                                                                                                                                                                                                                                                                                             cacG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACACACTTCCCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCCCGGTCTGtac  >  1:470282/1‑297 (MQ=17)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                             ATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGT‑GCAATACACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTC‑TTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCC  >  NC_000913/1096859‑1097456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑