Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	712,875	+C	intergenic (‑73/‑112)	ybfF ← / → seqA	acyl‑CoA esterase/negative modulator of initiation of replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	712,875	1	.	C	97.5%	280.3 / ‑0.7	79	intergenic (‑73/‑112)	ybfF/seqA	acyl‑CoA esterase/negative modulator of initiation of replication
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (46/31);  total (47/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑T‑AAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGTCACCTGCTATCG  >  NC_000913/712617‑713155                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                           cATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGcc                                                                                                                                                                                                                                                               <  1:718713/289‑1 (MQ=255) cATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGGTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATTTTGACTATTGTG‑CAGAGTGGCTGC‑AACAAC‑TCAAGGTTTATTCCGGGTTTTTGTGAAAGcc                                                                                                                                                                                                                                                               >  2:151382/1‑289 (MQ=255)         gATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTTTTCCGCGTTTTTCTGAAAGCCAGGCTTGa                                                                                                                                                                                                                                                       >  1:780404/1‑289 (MQ=255)                                        atCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGCTGGGAGAAGTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTAATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGACACAG‑GGCTGC‑AACGAA‑TCAAGGTTTCTTCCGAGTTTGTCTGCAAGCCAGTCTTGACGCCACGTGCTGCCggggtcatttcagctac                                                                                                                                                                                                                        >  2:465356/1‑275 (MQ=255)                                        atCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCatat                                                                                                                                                                                                                          >  1:332977/1‑287 (MQ=255)                                                  atgatATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAT‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCACGGCTTGACGCTATCCTCTGCGGGGATTTCTTCTTTTACTCCTTGCGat                                                                                                                                                                                                              >  2:499896/1‑288 (MQ=255)                                                       atTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCg                                                                                                                                                                                                                                              >  1:33616/1‑252 (MQ=255)                                                       atTGGGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCg                                                                                                                                                                                                                                              <  2:33616/252‑1 (MQ=255)                                                        ttGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTAt                                                                                                                                                                                                        <  1:114727/289‑1 (MQ=255)                                                        ttGTGATAGTTTACCAGGTCGGGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTAt                                                                                                                                                                                                        <  2:642606/289‑1 (MQ=255)                                                           tgATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCa                                                                                                                                                                                                     >  1:816351/1‑289 (MQ=255)                                                           tgATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCa                                                                                                                                                                                                     >  1:642606/1‑289 (MQ=255)                                                           tgATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGGTGTTGGGTTAGGGTATTATGTTGTCCATTGTGCCACCG‑GGGCGC‑AACAAATT‑AAGGTTTATTTCGAGTTTTTTAGCAAGCCAGGGTTGACGTTATTCGATTGCGGGATTTAATCCTTTACTTCTGGCGACTTGttttga                                                                                                                                                                                                     >  1:461164/1‑287 (MQ=255)                                                               gtggtACCAGATGGCGAGCCCGTGCGACAATGTGGTTGAGGCTGCCAAACAGAGCGTGGACAAGAAGGATGGGAGAATTATTGGTCTGTTTTTGGGCAGTTTGTGCGCGGATATTCAATTTCAGGGCAAAGTTCTTTTTTTCGCGTTGGCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTa                                                                                                                                                                                                 <  2:308709/285‑1 (MQ=255)                                                               cGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGTCCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTa                                                                                                                                                                                                 <  1:419794/289‑1 (MQ=255)                                                                gTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCTGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCGGGGATTTATTCTTATTCTCCTGGCGACTTGTCTTCAGCtga                                                                                                                                                                                                >  2:575554/1‑287 (MQ=255)                                                                gTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCCGGCTTGACGCTATCCGCTGCCGGGATTTATTACTATACTCCTTGCTCCTTGTATTCGACTaa                                                                                                                                                                                                >  2:482773/1‑289 (MQ=255)                                                                gTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGAGACTTGTATTCAGCTaa                                                                                                                                                                                                >  1:412952/1‑289 (MQ=255)                                                                  ttACCAGATCGCGAGCCAGTACGCCAAGGTGGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGa                                                                                                                                                                                              <  1:231178/289‑1 (MQ=255)                                                                         aTCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGAAAGCCAGGCTCGACGCTATCCGCTGCCGGGATTTATTCATATCATCCTGGCGACTTGTATTCAGCTAATacaccgt                                                                                                                                                                                        >  1:679584/1‑285 (MQ=255)                                                                            gcgAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTTTTCATCTAAATccctgccctg                                                                                                                                                                                    >  2:513632/1‑289 (MQ=255)                                                                                   aGTACGCCAAGGTTGTCGAGGCTGCAAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTCTGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCACGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCTCTGCCGGGATTTATTCATATACTCCTGGCGAATTGTATTCAGATAAGCAACTGCACTGGAttcat                                                                                                                                                                             >  1:350392/1‑286 (MQ=255)                                                                                        gCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAATAACGATGGGAGAATGATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGaa                                                                                                                                                                        <  1:580146/289‑1 (MQ=255)                                                                                        gCCAAGGTTGTCAGGGCTGCAAAACAGACAGTGGAAAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTGTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGaa                                                                                                                                                                        <  2:679584/289‑1 (MQ=255)                                                                                            aGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGCGTTTTTCTGCAAGCCAGTCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTTGCGAATTGTATTCCGCTAAGATACTGCTCTGGCTTAAGATGAAAACg                                                                                                                                                                    >  1:222818/1‑289 (MQ=255)                                                                                                  tCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTCTTCATATACTCCTGGCGACTTGTATTCAGCTAAGCCAATGCACTGGATTAAGATGAAAACGATTGaa                                                                                                                                                              >  1:136949/1‑289 (MQ=255)                                                                                                  tCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGCTTAAGATGAAAACGATTGaa                                                                                                                                                              >  1:331495/1‑289 (MQ=255)                                                                                                  tCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTAGGGTTAGGGTATTATGTTGACCATTGTGCAACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCAGAGGTTTTCTGCAAGCCAGGCTTGACGCTAGCGGCTGCCGGGTTTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACGGCACTGGATGACGATGAACACGATTGaa                                                                                                                                                              >  2:181591/1‑289 (MQ=255)                                                                                                                    ccGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTa                                                                                                                                             <  1:513632/288‑1 (MQ=255)                                                                                                                        ggACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCt                                                                                                                                        >  1:249997/1‑289 (MQ=255)                                                                                                                             aGAACGATGGGGGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTGTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATAtt                                                                                                                                   <  1:57782/289‑1 (MQ=255)                                                                                                                                   aTGGGAGAATTTTTGGGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTgccagc                                                                                                                             <  2:141251/289‑1 (MQ=255)                                                                                                                                   aTGAGATAATTATTGTGCTGGTTTTGTGGAGTTTGCGCTCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTgccagc                                                                                                                             <  2:68827/289‑1 (MQ=255)                                                                                                                                    tGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTgccagc                                                                                                                             <  2:816351/288‑1 (MQ=255)                                                                                                                                                    cTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGACACACGACGAATATCg                                                                                                             >  2:64891/1‑288 (MQ=255)                                                                                                                                                       gTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGATACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCga                                                                                                         >  2:317381/1‑289 (MQ=255)                                                                                                                                                       gTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACAATAAGCATATCGGCga                                                                                                         >  1:214195/1‑289 (MQ=255)                                                                                                                                                       gTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCAAACTAAGCATAACGGCga                                                                                                         >  1:70885/1‑289 (MQ=255)                                                                                                                                                       gTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGACAGCCACACTAAGCATATCGgaga                                                                                                         >  1:107595/1‑289 (MQ=255)                                                                                                                                                         tttGTGCAGTTTGGGCGCGGATATTCAATTTCATTGACATGGTCTTTTTTTCGGTTTGTCGGGTTATGGTATTAACTTGACCCTTTGGCCACAG‑GGCTTC‑AACCAA‑TCAATGTTTATTCCTAGTTTTTCTGCAAGCCAGGCTTGCCGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCGCTGGATTAAGATGAAAACGATTGAAGTTGAGGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCgaga                                                                                                       <  2:478696/289‑1 (MQ=255)                                                                                                                                                          ttGTGCAGTTTGCGCGCGGATATTCAATTTAATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGagag                                                                                                      <  1:64891/289‑1 (MQ=255)                                                                                                                                                            gtgCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTgccagc                                                                                                                             <  2:527388/264‑1 (MQ=255)                                                                                                                                                            gtgCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTgccagc                                                                                                                             >  1:527388/1‑264 (MQ=255)                                                                                                                                                                  tttGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGaca                                                                                                                                                                                            <  1:453931/195‑1 (MQ=255)                                                                                                                                                                  tttGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGaca                                                                                                                                                                                            >  2:453931/1‑195 (MQ=255)                                                                                                                                                                  tttGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCg                                                                                              <  2:74521/289‑1 (MQ=255)                                                                                                                                                                   ttGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGATACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATc                                                                                                <  1:317381/286‑1 (MQ=255)                                                                                                                                                                                          tGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCAAACCTAAGCATATCAGGGAGATCGCAACAGACATGTTACGGCGTAGGTTGAAAt                                                                      >  2:303825/1‑289 (MQ=255)                                                                                                                                                                                                tggtCTTTTTTTACCGTTGTCGGGTTAGGGTATTATGTTGACCAATGTTCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTccg                                                                <  2:332977/286‑1 (MQ=255)                                                                                                                                                                                                aGTTTTTTTTTTGGCGTTGTGGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGA‑AACAAA‑TCAAGGTGTATGCCGAGTTGTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTccg                                                                <  2:331495/289‑1 (MQ=255)                                                                                                                                                                                                   tCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTAAGTTGAAATTTTccgc                                                               <  1:575554/287‑1 (MQ=255)                                                                                                                                                                                                     tttttttCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCa                                                           >  1:182261/1‑289 (MQ=255)                                                                                                                                                                                                            cgcgTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCC‑T‑CACAg                                                   >  1:757763/1‑288 (MQ=255)                                                                                                                                                                                                               gTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCctg                                               <  1:138344/289‑1 (MQ=255)                                                                                                                                                                                                                  gTCGGGTTAGTGTATTATGTTGACCATTTTGCCACAC‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCTAGTTTTTCTTCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATGCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCctgctg                                            <  2:136949/289‑1 (MQ=255)                                                                                                                                                                                                                         tAGTGTATTATTTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGt                                     <  1:470702/289‑1 (MQ=255)                                                                                                                                                                                                                         tAGGTTATTATGTTGCCCATTCGGCCCCCG‑GGCTGC‑AACAAA‑TCATTGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGt                                     <  1:181591/289‑1 (MQ=255)                                                                                                                                                                                                                                ttatGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTGTATTCCGAGGTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGaaa                              <  1:711054/289‑1 (MQ=255)                                                                                                                                                                                                                                 tatGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAag                             >  1:679716/1‑289 (MQ=255)                                                                                                                                                                                                                                 tatGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAag                             >  1:465756/1‑289 (MQ=255)                                                                                                                                                                                                                                 tatGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑ACCAGCCTGCTGCTCCGGTGACGaacg                             >  2:813029/1‑287 (MQ=255)                                                                                                                                                                                                                                       gACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGCGGttt                       >  1:222141/1‑288 (MQ=255)                                                                                                                                                                                                                                       gACCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCAAGCAACACTAAGCATATCGGCGAGAGCGAATCCGACATTTTACGGCGTAGGTTGAAATTTTCCGCTGCA‑T‑CACCACCTGCTGCTCCGGTGACGAAAGAGGTTc                       >  2:482450/1‑289 (MQ=255)                                                                                                                                                                                                                                        aCCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCTACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTCCCAGCCATACTACGCATATCGGCCATAGCGCCTCCGACATTTTACGGCGTATGTTGAAATTTTCCGCC‑CA‑TACACAGCCTTCTTATCCGGTGACGAAAGAGGTTc                       >  2:162167/1‑288 (MQ=255)                                                                                                                                                                                                                                        aCCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCTAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CCCAGCCTGCTGCTCCGGTGACGAAAGAGGTTc                       >  1:593245/1‑288 (MQ=255)                                                                                                                                                                                                                                        aCCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTc                       >  1:434362/1‑288 (MQ=255)                                                                                                                                                                                                                                        aCCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCAAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCC‑T‑CACCACCTGCTGCTCCGGTTACGAAAGATGTTc                       >  2:687925/1‑288 (MQ=255)                                                                                                                                                                                                                                        aCCATTGTGCCACAG‑GGCTGC‑AACAAA‑TCAAGGTGTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGATATCCGCTGCCGGGATGAATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGAATTAAGATGAAAACGATTGAAGTTGATACTGAACGCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGCGAGCGCATCAGAGAGTTTACGGCGTAGTGTGCAATTTTCC‑CCACACT‑CACCGCCTGCTGCGCCGGTGACGGACGATGTTc                       >  2:218267/1‑288 (MQ=255)                                                                                                                                                                                                                                            ttggtccACAG‑GGCTTC‑AACAAA‑TCAAGGTTTTTTCCGATTTTTTCTGAAATCCACGCTTTACGCTATCAGCTGCCGGGATTTATTCATATACTCCTGTCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGAGGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGtt                  <  2:222818/284‑1 (MQ=255)                                                                                                                                                                                                                                             tgtgCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTGAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGACACACTAAGCAAATCGGCGAGAGCGCCTCCGACATTTTACGGCGTATGATGAAATTTTCAGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAACGAGGTTCTCGtta                 >  1:627669/1‑288 (MQ=255)                                                                                                                                                                                                                                             tgtgCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGCGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGtt                  >  1:247145/1‑288 (MQ=255)                                                                                                                                                                                                                                             tgtgCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGtt                  >  1:402084/1‑288 (MQ=255)                                                                                                                                                                                                                                             tgggCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTg                 <  1:151382/286‑1 (MQ=255)                                                                                                                                                                                                                                              gtgCCACAG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTTACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGc                <  2:402084/289‑1 (MQ=255)                                                                                                                                                                                                                                                gtcacaG‑GGCTGCAAACAAG‑C‑AAGGTTTATTCCGAGTTTTCCTGCAAGCTAGGCTTGACGCTATCCGCTGCCTGGATTTAATCATATACTCCTGGCGAGTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGt              <  2:438451/287‑1 (MQ=255)                                                                                                                                                                                                                                                    caG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCTTGCTGCCCCGGTGACGAAAGAGGTTCGCGTTGCGTccg           >  2:84205/1‑286 (MQ=255)                                                                                                                                                                                                                                                    caG‑GGCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGTCa            >  1:214849/1‑287 (MQ=255)                                                                                                                                                                                                                                                        ggCTGC‑AACAAA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGACTGCTGCTCCGGTGACGCAAGAGGTTCGCGTTgatgcacctt        >  1:522160/1‑279 (MQ=255)                                                                                                                                                                                                                                                             c‑aacaaA‑TCAAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCAAACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGAAGAAAGAGGTTCGCGTGGCGTCACCTGcattcg  >  1:418869/1‑284 (MQ=255)                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                           CATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGGTTGTCGAGGCTGCCAAACAGACCGTGGACAAGAACGATGGGAGAATTATTGTGCTGGTTTTGTGCAGTTTGCGCGCGGATATTCAATTTCATGGCAAAGTTCTTTTTTTCGCGTTGTCGGGTTAGGGTATTATGTTGACCATTGTGCCACAG‑GGCTGC‑AACAAA‑T‑AAGGTTTATTCCGAGTTTTTCTGCAAGCCAGGCTTGACGCTATCCGCTGCCGGGATTTATTCATATACTCCTGGCGACTTGTATTCAGCTAAGACACTGCACTGGATTAAGATGAAAACGATTGAAGTTGATGATGAACTCTACAGCTATATTGCCAGCCACACTAAGCATATCGGCGAGAGCGCATCCGACATTTTACGGCGTATGTTGAAATTTTCCGCCGCA‑T‑CACAGCCTGCTGCTCCGGTGACGAAAGAGGTTCGCGTTGCGTCACCTGCTATCG  >  NC_000913/712617‑713155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑