Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BX571857 605,157 T→C 6.6% D1007D (GAT→GAC SAS0521 → bone sialoprotein‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BX571857605,1570TC 6.6% 609.2 / 9.8 179D1007D (GAT→GACSAS0521bone sialoprotein‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (83/81);  new base C (11/3);  total (94/85)
Fisher's exact test for biased strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

CTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605061‑605253
                                                                                                |                                                                                                
ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                  >  2:908926/1‑97 (MQ=255)
ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                  >  2:2212068/1‑97 (MQ=255)
ctcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAt                                                                                                  >  1:2569536/1‑97 (MQ=255)
 tcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 >  1:2312081/1‑97 (MQ=255)
 gcagactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                 <  2:1391047/96‑1 (MQ=255)
    gactcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAg                                                                                              <  2:2036346/97‑1 (MQ=40)
     actcagaTAGTGATTCAGGTTCCGATAGCGACTCAGCCTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  2:2025706/97‑1 (MQ=14)
     actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  1:865940/97‑1 (MQ=255)
     actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  1:2640850/97‑1 (MQ=255)
     actcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                             <  1:1754481/97‑1 (MQ=255)
      ctcagaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                            >  2:1618814/1‑97 (MQ=255)
         agaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                         <  1:2368304/97‑1 (MQ=255)
         agaTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                         <  2:2386786/97‑1 (MQ=255)
           aTAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                       <  2:95857/97‑1 (MQ=39)
           aTAGTGATTCAGAATCAGACAGCGACGCAGCATCAGACAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                       <  2:511302/97‑1 (MQ=11)
               tGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  1:313592/97‑1 (MQ=40)
               ggattcagactcaGATAGCGACTCAGACTCATATAGCGACTCAGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                   <  2:315341/96‑1 (MQ=14)
                gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  >  1:1681727/1‑97 (MQ=255)
                gattcagattcaGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                  >  2:1756849/1‑97 (MQ=21)
                 attcagattcagATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                 >  1:368045/1‑97 (MQ=255)
                  ttcagattcagaTAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                >  1:596544/1‑97 (MQ=255)
                   tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACt                                                                               <  2:2288485/97‑1 (MQ=255)
                    ccgattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                              <  2:286448/95‑1 (MQ=255)
                    cagagtcagacAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                              <  2:419526/97‑1 (MQ=255)
                     agattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCa                                                                             >  1:2066534/1‑97 (MQ=255)
                      gattcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                            <  1:2289016/97‑1 (MQ=255)
                       actcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                           <  1:1361130/95‑1 (MQ=255)
                       actcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                           <  1:663312/95‑1 (MQ=255)
                        ttcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAt                                                                          >  1:2207019/1‑97 (MQ=17)
                         tcagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATa                                                                         <  1:636461/97‑1 (MQ=255)
                          cagatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                        >  1:1670449/1‑97 (MQ=255)
                           agatAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc                                                                       <  1:448435/97‑1 (MQ=255)
                            gacaGCGACCCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                      <  2:2506272/94‑1 (MQ=255)
                              tAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGAc                                                                    >  2:2524879/1‑97 (MQ=21)
                               aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGAcc                                                                   >  1:1990213/1‑96 (MQ=255)
                               aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACt                                                                   >  2:2412520/1‑97 (MQ=255)
                               aGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACt                                                                   >  2:44752/1‑97 (MQ=25)
                                 cGACTCAGACTCCGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                 <  2:242982/97‑1 (MQ=255)
                                 cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                 <  1:2353719/97‑1 (MQ=255)
                                 cGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCa                                                                 <  1:729706/97‑1 (MQ=255)
                                  gactcagactcaGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                >  1:1559980/1‑97 (MQ=255)
                                  gactcagactcaGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                <  1:1167167/97‑1 (MQ=255)
                                  gactcagactcaGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAg                                                                <  1:618001/97‑1 (MQ=255)
                                   actcagactcagATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGa                                                               <  1:2137828/97‑1 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGact                                                             >  2:1272075/1‑95 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             >  2:1477496/1‑97 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             >  2:2350527/1‑97 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             >  1:218338/1‑97 (MQ=255)
                                     tcagactcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGact                                                             >  2:880047/1‑95 (MQ=255)
                                     ccagactcagaCCGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAtt                                                             <  2:1230025/96‑1 (MQ=14)
                                       agactcagaTAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCa                                                           <  2:649988/97‑1 (MQ=255)
                                           tcagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                       <  1:94410/97‑1 (MQ=255)
                                           ccagaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACa                                                       <  2:1028761/96‑1 (MQ=255)
                                             agaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGt                                                     >  2:58421/1‑97 (MQ=255)
                                              gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  1:837023/97‑1 (MQ=255)
                                              gaTAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTg                                                    <  1:1449503/97‑1 (MQ=255)
                                                tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgac                                                  <  1:1272240/97‑1 (MQ=255)
                                                 aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact                                                 >  2:1259248/1‑97 (MQ=255)
                                                    gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              >  2:2254245/1‑97 (MQ=255)
                                                    gACTCAGAATCGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcag                                              <  2:1133157/97‑1 (MQ=255)
                                                      cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagac                                            >  2:1177707/1‑97 (MQ=255)
                                                       tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagact                                           >  1:1784475/1‑97 (MQ=255)
                                                        cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactc                                          >  2:1587446/1‑97 (MQ=255)
                                                         aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                         <  1:1705137/97‑1 (MQ=255)
                                                         aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTgactcagactca                                         <  1:788014/97‑1 (MQ=255)
                                                          gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGactcagactcag                                        >  1:957823/1‑97 (MQ=255)
                                                           aTTCGGACAGCGATTCAGACTCAGATAGCGACGCAGATTCAGACAGCGACTCAGACTCAGATAGAGACTCAGCTTCAGACAGTGaccctgactcagg                                       >  2:822117/1‑96 (MQ=11)
                                                            ttCGGACGGCGATGCAAACTCAGACAGCGACTCAGAATCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGAt                                      <  2:1113548/97‑1 (MQ=14)
                                                             tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATa                                     >  2:1895655/1‑97 (MQ=255)
                                                                gACAGCGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCg                                  <  2:1826081/97‑1 (MQ=255)
                                                                 aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCga                                 >  1:2099233/1‑97 (MQ=255)
                                                                 aCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCga                                 >  1:2623208/1‑97 (MQ=255)
                                                                  cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgac                                <  2:2471191/97‑1 (MQ=255)
                                                                   aGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCgact                               >  2:574591/1‑97 (MQ=255)
                                                                      gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcag                            >  1:2538036/1‑97 (MQ=255)
                                                                           aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactca                       <  1:1120331/97‑1 (MQ=255)
                                                                           aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCgactcagactca                       <  1:2090177/97‑1 (MQ=255)
                                                                           aGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTAAGACTCAGATAGCgactcagactca                       >  2:806238/1‑97 (MQ=255)
                                                                             aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGActcagactcaga                     <  2:1676264/97‑1 (MQ=255)
                                                                               tCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACa                   <  1:243529/97‑1 (MQ=255)
                                                                                cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAg                  >  1:2158657/1‑97 (MQ=255)
                                                                                 aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:1224080/97‑1 (MQ=255)
                                                                                 aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:531312/97‑1 (MQ=255)
                                                                                 aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGt                 <  1:1019838/97‑1 (MQ=255)
                                                                                  gATAGCGACTCAGATTCGGACAGCGATTCAGCCTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGtt                <  2:1357978/97‑2 (MQ=255)
                                                                                  gATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  1:1804254/1‑97 (MQ=255)
                                                                                    tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGAGACTCAGACCCAGATAGCGACCCCGACTCAGACAGCgac              >  2:1247743/1‑97 (MQ=11)
                                                                                     aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgact             >  2:264487/1‑97 (MQ=255)
                                                                                     aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgacc             >  2:2492945/1‑96 (MQ=255)
                                                                                       cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  2:170264/97‑1 (MQ=255)
                                                                                       cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  2:708425/97‑1 (MQ=255)
                                                                                       cGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactca           <  1:1343346/97‑1 (MQ=255)
                                                                                        gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcag          <  2:2642933/97‑1 (MQ=255)
                                                                                           tCAGATTCAGACGGCGATTCAGCCTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact       <  2:1542796/97‑1 (MQ=255)
                                                                                           tCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagact       >  1:1410507/1‑97 (MQ=255)
                                                                                             aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     >  2:475025/1‑97 (MQ=255)
                                                                                             aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  1:1906517/97‑1 (MQ=255)
                                                                                             aGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgactcagactca     <  1:1023030/97‑1 (MQ=255)
                                                                                               aTTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGActcggactcaga   <  1:251572/97‑1 (MQ=255)
                                                                                                ttCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACtcagactcagac  >  1:2649589/1‑97 (MQ=255)
                                                                                                |                                                                                                
CTCAGACTCAGATAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGAC  >  BX571857/605061‑605253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: