| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BX571857 | 1,035,394 | A→T | 8.1% | intergenic (+392/‑249) | SAS0963 → / → SAS0964 | hypothetical protein/putative exported protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BX571857 | 1,035,394 | 0 | A | T | 8.1% | 341.9 / 11.3 | 83 | intergenic (+392/‑249) | SAS0963/SAS0964 | hypothetical protein/putative exported protein |
| Reads supporting (aligned to +/- strand): ref base A (47/29); new base T (2/5); total (49/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAAGTTCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTGCGC > BX571857/1035299‑1035490 | aTGAAGTTCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAAc > 1:2037123/1‑97 (MQ=255) aaGTTCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGaa < 1:1474259/97‑1 (MQ=255) aGTTCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAat < 2:2042101/97‑1 (MQ=255) tCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAatgat < 1:1678866/97‑1 (MQ=255) cATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAatgatg < 1:831046/97‑1 (MQ=255) cATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAatgatg > 1:1843198/1‑97 (MQ=255) atatatTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATgg > 1:1012934/1‑97 (MQ=255) atatatTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATgg > 1:772903/1‑97 (MQ=255) atatTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGtt > 1:2616065/1‑97 (MQ=255) tatTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTa < 1:456959/97‑1 (MQ=255) tatTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTa < 1:456971/97‑1 (MQ=255) ttAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTaaa > 1:342463/1‑97 (MQ=255) ttAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTaaa > 2:1267577/1‑97 (MQ=255) ttAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTaaa > 1:1595136/1‑97 (MQ=255) tAATATAACTTTCATTGATATAGAAGTCGCANTGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAg > 1:1438266/1‑97 (MQ=255) tAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAg > 2:845573/1‑97 (MQ=255) ataACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGaaaa < 2:1825168/97‑1 (MQ=255) taACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAAt < 2:1038596/97‑1 (MQ=255) cgcTTTCATTGGTATAGTAGGCGCAATGATAAATTTAAGCAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAGAtc < 2:716495/95‑1 (MQ=11) aaCTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAAtc > 2:1107651/1‑97 (MQ=255) cTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAAtctc < 2:1566428/97‑1 (MQ=255) tttCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCa > 1:1423959/1‑97 (MQ=255) tttCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCa > 1:1423946/1‑97 (MQ=255) tttCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCa > 1:369474/1‑97 (MQ=255) tCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCAtt < 2:2472732/97‑1 (MQ=255) tCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCAtt < 2:2472829/97‑1 (MQ=255) cATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAATGAATGATGGTTAAAGAAAATCTCAttt < 1:1169326/97‑1 (MQ=255) aTTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTa > 2:1049887/1‑97 (MQ=255) gATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTc > 1:1233293/1‑97 (MQ=255) taGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATATCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCg > 2:2164604/1‑97 (MQ=255) gAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTc > 2:611846/1‑97 (MQ=255) gAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTc > 2:2376965/1‑97 (MQ=255) cGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCttttt > 1:2552713/1‑97 (MQ=255) cGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACAGTCttttt > 2:1660743/1‑97 (MQ=255) aaTGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAAc > 1:2478489/1‑97 (MQ=255) tGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACgg < 1:2357104/97‑1 (MQ=255) tGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACgg > 2:1819290/1‑97 (MQ=255) tGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACgg > 2:2589726/1‑97 (MQ=255) tGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACgg > 2:2589827/1‑97 (MQ=255) gATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACggg > 2:266743/1‑97 (MQ=255) aTTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGt > 1:1233113/1‑97 (MQ=255) ttAATTTAAACAATAAAAAACACCCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGtt < 1:1195770/97‑1 (MQ=255) ttAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGtt > 2:496166/1‑97 (MQ=255) ttAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATa > 1:1388402/1‑97 (MQ=255) ttAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATa > 2:2567390/1‑97 (MQ=255) ttAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATa > 2:258557/1‑97 (MQ=255) tAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAg > 2:291676/1‑97 (MQ=255) tAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAg > 2:1448384/1‑97 (MQ=255) aagcAATAAAAAATAACCATCATTTCAAAACTTTGACATCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAgg > 2:2274795/4‑97 (MQ=1) cAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGa > 2:1418295/1‑97 (MQ=255) aaTAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGAc > 2:407484/1‑97 (MQ=255) aaTAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGAc > 1:1610011/1‑97 (MQ=255) aaTAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGAc > 1:331140/1‑97 (MQ=255) aTAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACa > 1:1001287/1‑97 (MQ=255) aTAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGATTAAAGAAAATCTCATTTAGTCACCGTCTATTTAACGGGTTCAATAGGAGACa > 1:680278/1‑97 (MQ=255) tAAAAAACAACCATCATTTCAAAACTTTGACATAGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACAt < 2:580538/97‑1 (MQ=255) aaaaCAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGtt < 1:1138418/97‑1 (MQ=255) aaaaCAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGtt > 1:61658/1‑97 (MQ=255) aaaaCAACCATCATTTCAAAACTTTGACAACGAATGAGGGTTAAAGAAAAGCTCAGTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGtt < 2:896006/97‑1 (MQ=255) aaaCAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTc < 1:89308/97‑1 (MQ=255) aaaCAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTc > 2:2344201/1‑97 (MQ=255) aacaacCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCAttt < 1:664459/56‑1 (MQ=255) aacaacCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTcc > 2:2479872/1‑97 (MQ=255) caacCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTg > 1:2337822/1‑97 (MQ=255) caacCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTg > 2:1029857/1‑97 (MQ=255) aacCATCATTTCAAAACTTTGACATAGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACAt < 2:1595461/89‑1 (MQ=255) aacCATCATTTCAAAACTTTGACATAGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACAt > 1:1595462/1‑89 (MQ=255) acCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCa < 1:2274796/97‑1 (MQ=255) atTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATgtcgt > 1:259463/1‑97 (MQ=255) atTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATgtcgt > 1:733597/1‑97 (MQ=255) tCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTa < 2:1157495/97‑1 (MQ=255) tCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTa < 2:1157078/97‑1 (MQ=255) aaaaCTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTAtt < 1:114490/97‑1 (MQ=255) aaaaCTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTAtt < 1:1025381/97‑1 (MQ=255) aaaaCTTTGACAACGAATGATGGCTAAAGAAAACCTCATTTAGTCACCGTCTGTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTAtt < 2:1282015/97‑1 (MQ=255) aaCTTTGACATCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTa < 2:2144158/97‑1 (MQ=255) aaCTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTa < 1:45413/97‑1 (MQ=255) tttGACATCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGc < 2:626107/97‑1 (MQ=255) tttGACATAGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGCGACATGTTCCTGCATGTCGTCTATTTATGc < 2:2001516/97‑1 (MQ=255) tttGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGc < 1:1764587/97‑1 (MQ=255) tttGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGc < 1:1189134/97‑1 (MQ=255) gACAACGCTTGATGGTTCAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACt < 2:1621353/97‑1 (MQ=255) cAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTgc > 1:1561768/1‑97 (MQ=255) aaCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTgcg > 1:1749484/1‑97 (MQ=255) aCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTgcgc < 1:327215/97‑1 (MQ=255) aCGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTgcgc < 1:2511352/97‑1 (MQ=255) | ATGAAGTTCATATATTAATATAACTTTCATTGATATAGAAGTCGCAATGATTAATTTAAACAATAAAAAACAACCATCATTTCAAAACTTTGACAACGAATGATGGTTAAAGAAAATCTCATTTAGTCACCGTCTTTTTAACGGGTTCAATAGGGGACATGTTCCTGCATGTCGTCTATTTATGCACTGCGC > BX571857/1035299‑1035490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |